Sp100 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sp100
Entrez Gene ID	363269
Full Name	SP100 nuclear antigen
General protein information	Preferred Names SP100 nuclear antigen Names nuclear autoantigen Sp-100 nuclear antigen Sp100
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 9 Map Location: 9q35

mRNA and Protein(s)

mRNA	Protein	Name
NM_001014220.1	NP_001014242.1	nuclear autoantigen Sp-100

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Sp100	NP_038701.2
Rattus norvegicus (Norway rat)	Sp100	NP_001014242.1

Gene Ontology
Bibliography

Related articles in PubMed

Pooled-matrix protein interaction screens using Barcode Fusion Genetics.
Yachie N, Petsalaki E, Mellor JC, Weile J, Jacob Y, Verby M, Ozturk SB, Li S, Cote AG, Mosca R, Knapp JJ, Ko M, Yu A, Gebbia M, Sahni N, Yi S, Tyagi T, Sheykhkarimli D, Roth JF, Wong C, Musa L, Snider J, Liu YC, Yu H, Braun P, Stagljar I, Hao T, Calderwood MA, Pelletier L, Aloy P, Hill DE, Vidal M, Roth FP
Molecular systems biology12(4)863(2016 Apr)

Purification of proteins associated with specific genomic Loci.
D?jardin J, Kingston RE
Cell136(1)175-86(2009 Jan)

Dynamic regulation of p53 subnuclear localization and senescence by MORC3.
Takahashi K, Yoshida N, Murakami N, Kawata K, Ishizaki H, Tanaka-Okamoto M, Miyoshi J, Zinn AR, Shime H, Inoue N
Molecular biology of the cell18(5)1701-9(2007 May)

FLASH links the CD95 signaling pathway to the cell nucleus and nuclear bodies.
Milovic-Holm K, Krieghoff E, Jensen K, Will H, Hofmann TG
The EMBO journal26(2)391-401(2007 Jan)

Differential role of Sp100 isoforms in interferon-mediated repression of herpes simplex virus type 1 immediate-early protein expression.
Negorev DG, Vladimirova OV, Ivanov A, Rauscher F 3rd, Maul GG
Journal of virology80(16)8019-29(2006 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Sp100 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sp100 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa05426	NM_001014220.1 Latest version!	Rattus norvegicus SP100 nuclear antigen (Sp100), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sp100 ( NM_001014220.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001014242.1 Rattus norvegicus SP100 nuclear antigen (Sp100), mRNA.

Price & Availability↑

CloneID	ORa05426
Clone ID Related Accession (Same CDS sequence)	NM_001014220.1
Accession Version	NM_001014220.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1029bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-30
Organism	Rattus norvegicus(Norway rat)
Product	nuclear autoantigen Sp-100
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC083808.1. On Apr 1, 2005 this sequence version replaced XM_343611.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: BC083808.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGAAGGGA ACAGCTTCAG CTCCAGGATG TCCAGTGAAC ATGAGAATAC AGAGACGTTT 
CCCTTTGAAC TTATATTTCA ACACTTCAAG AGACAGAAAG TGGCAATTTC TAATGCAATA 
AAAAATCCAT TTCCTTTTCT TGAGGGCCTC CGAGATCATG AATTAATCAC TGCTAAAATG 
TATGAAGATC TTCAAGATTC GTATAGAGCC CTGGTCCGTG TACAGGAAGT GATCTACAGA 
GCCCTGGATA AACTGGAGAA GATATTCAGC ATGAAGGTCC TCTATGAAGT ATTCAGTAAT 
GTCAACATGG AGAAATATCC AGATTTGCAA CTCATTTATC AAAGCTTTGA ACGTGTGTTA 
CCAAATGGAT TGTGTTTCCA AAGGAGTGAT GGAGGAGACC CAAACTCCCA GCTGAGTCTT 
GAACAAGGAC CTAATGGCAG CTGTTCTCAA GCAACCCTGA CCTTGTCACC CTTCAATCAC 
TCCTTTTCTG ACGGCTGGGG AAGTAATGAT AGACTAAATT CCAGCCTCAC CCATGGAAAC 
CAAAGTGAGA ATCATCAACT CCCCGGATCA CCAAATCACC TTGGAGCTGT GCAACTCAAC 
CATGACCTCC AAATTAATCC CTGTTCTGTG CGGCTGGTAG ATATAAAGAA GGAAAATATA 
TCATATTCTT TGGATGATAA CCAACAGACC CAAGCAAGGA CTAACGAAAA TCAGGGCTCT 
GAGATAATAG AGCTCAGCAG TGATGAGGAT TCTAATGATG AAATCAACTT GTCAGAAGCC 
TCTACCTTAA TCCTCTCCAA GCCAGAGACT GCACATTCCA GACAACCTCC CACACCAAGA 
ACATACAGAA GAGGAGATAC TTCTGATACC AGCAGCTCAA GCCTCGATAG AAGACGGAAA 
AGGACAGGAC GAATGTTGAG TAGCTCCTCT TCTGAACTGA GTTCTGGAGA GGAATCTGAG 
GAATTTCTAA GAAGTGGATC AGGAAGATTA GGATGCCCAG CCTCGGCCCA CTGGATGGCA 
GGAGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001014242.1
CDS	4..1032
Translation	MEGNSFSSRMSSEHENTETFPFELIFQHFKRQKVAISNAIKNPFPFLEGLRDHELITAKMYEDLQDSYRALVRVQEVIYRALDKLEKIFSMKVLYEVFSNVNMEKYPDLQLIYQSFERVLPNGLCFQRSDGGDPNSQLSLEQGPNGSCSQATLTLSPFNHSFSDGWGSNDRLNSSLTHGNQSENHQLPGSPNHLGAVQLNHDLQINPCSVRLVDIKKENISYSLDDNQQTQARTNENQGSEIIELSSDEDSNDEINLSEASTLILSKPETAHSRQPPTPRTYRRGDTSDTSSSSLDRRRKRTGRMLSSSSSELSSGEESEEFLRSGSGRLGCPASAHWMAGV

Target ORF information:

RefSeq Version	NM_001014220.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus SP100 nuclear antigen (Sp100), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001014220.1

ORF Insert Sequence:

ATGGAAGGGA ACAGCTTCAG CTCCAGGATG TCCAGTGAAC ATGAGAATAC AGAGACGTTT 
CCCTTTGAAC TTATATTTCA ACACTTCAAG AGACAGAAAG TGGCAATTTC TAATGCAATA 
AAAAATCCAT TTCCTTTTCT TGAGGGCCTC CGAGATCATG AATTAATCAC TGCTAAAATG 
TATGAAGATC TTCAAGATTC GTATAGAGCC CTGGTCCGTG TACAGGAAGT GATCTACAGA 
GCCCTGGATA AACTGGAGAA GATATTCAGC ATGAAGGTCC TCTATGAAGT ATTCAGTAAT 
GTCAACATGG AGAAATATCC AGATTTGCAA CTCATTTATC AAAGCTTTGA ACGTGTGTTA 
CCAAATGGAT TGTGTTTCCA AAGGAGTGAT GGAGGAGACC CAAACTCCCA GCTGAGTCTT 
GAACAAGGAC CTAATGGCAG CTGTTCTCAA GCAACCCTGA CCTTGTCACC CTTCAATCAC 
TCCTTTTCTG ACGGCTGGGG AAGTAATGAT AGACTAAATT CCAGCCTCAC CCATGGAAAC 
CAAAGTGAGA ATCATCAACT CCCCGGATCA CCAAATCACC TTGGAGCTGT GCAACTCAAC 
CATGACCTCC AAATTAATCC CTGTTCTGTG CGGCTGGTAG ATATAAAGAA GGAAAATATA 
TCATATTCTT TGGATGATAA CCAACAGACC CAAGCAAGGA CTAACGAAAA TCAGGGCTCT 
GAGATAATAG AGCTCAGCAG TGATGAGGAT TCTAATGATG AAATCAACTT GTCAGAAGCC 
TCTACCTTAA TCCTCTCCAA GCCAGAGACT GCACATTCCA GACAACCTCC CACACCAAGA 
ACATACAGAA GAGGAGATAC TTCTGATACC AGCAGCTCAA GCCTCGATAG AAGACGGAAA 
AGGACAGGAC GAATGTTGAG TAGCTCCTCT TCTGAACTGA GTTCTGGAGA GGAATCTGAG 
GAATTTCTAA GAAGTGGATC AGGAAGATTA GGATGCCCAG CCTCGGCCCA CTGGATGGCA 
GGAGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Pooled-matrix protein interaction screens using Barcode Fusion Genetics.
Molecular systems biology12(4)863(2016 Apr)
Yachie N,Petsalaki E,Mellor JC,Weile J,Jacob Y,Verby M,Ozturk SB,Li S,Cote AG,Mosca R,Knapp JJ,Ko M,Yu A,Gebbia M,Sahni N,Yi S,Tyagi T,Sheykhkarimli D,Roth JF,Wong C,Musa L,Snider J,Liu YC,Yu H,Braun P,Stagljar I,Hao T,Calderwood MA,Pelletier L,Aloy P,Hill DE,Vidal M,Roth FP

Purification of proteins associated with specific genomic Loci.
Cell136(1)175-86(2009 Jan)
D?jardin J,Kingston RE

Dynamic regulation of p53 subnuclear localization and senescence by MORC3.
Molecular biology of the cell18(5)1701-9(2007 May)
Takahashi K,Yoshida N,Murakami N,Kawata K,Ishizaki H,Tanaka-Okamoto M,Miyoshi J,Zinn AR,Shime H,Inoue N

FLASH links the CD95 signaling pathway to the cell nucleus and nuclear bodies.
The EMBO journal26(2)391-401(2007 Jan)
Milovic-Holm K,Krieghoff E,Jensen K,Will H,Hofmann TG

Differential role of Sp100 isoforms in interferon-mediated repression of herpes simplex virus type 1 immediate-early protein expression.
Journal of virology80(16)8019-29(2006 Aug)
Negorev DG,Vladimirova OV,Ivanov A,Rauscher F 3rd,Maul GG