Stt3b cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Stt3b
Entrez Gene ID	363160
Full Name	STT3B, catalytic subunit of the oligosaccharyltransferase complex
Synonyms	RGD1311563
General protein information	Preferred Names STT3B, catalytic subunit of the oligosaccharyltransferase complex Names dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B STT3B, subunit of the oligosaccharyltransferase complex (catalytic) source of immunodominant MHC-associated peptides
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 8 Map Location: 8q32

mRNA and Protein(s)

mRNA	Protein	Name
NM_001170539.1	NP_001164010.1	dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B

Pathways from BioSystems

Homologies

Bos taurus (cattle)	STT3B	NP_001091039.1
Saccharomyces cerevisiae (baker's yeast)	STT3	NP_011493.1
Schizosaccharomyces pombe (fission yeast)	stt3	NP_595148.1
Magnaporthe oryzae (rice blast fungus)	MGG_04773	XP_003713725.1
Neurospora crassa	NCU10497	XP_001728408.1
Pan troglodytes (chimpanzee)	STT3B	XP_001166939.1
Rattus norvegicus (Norway rat)	Stt3b	NP_001164010.1
Gallus gallus (chicken)	STT3B	NP_001026684.1
Caenorhabditis elegans (roundworm)	stt-3	NP_498362.1
Kluyveromyces lactis	KLLA0C11627g	XP_452719.1
Eremothecium gossypii	AGOS_AFR491W	NP_986038.1
Homo sapiens (human)	STT3B	NP_849193.1
	STT3B	XP_001096091.1
Canis lupus familiaris (dog)	STT3B	XP_542747.2
Mus musculus (house mouse)	Stt3b	NP_077184.2
Drosophila melanogaster (fruit fly)	OstStt3	NP_524494.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002396	XP_312559.4
Xenopus tropicalis (tropical clawed frog)	stt3b	XP_002941861.2

Gene Ontology
Bibliography

Related articles in PubMed

ZMPSTE24 defends against influenza and other pathogenic viruses.
Fu B, Wang L, Li S, Dorf ME
The Journal of experimental medicine214(4)919-929(2017 Apr)

STT3B-dependent posttranslational N-glycosylation as a surveillance system for secretory protein.
Sato T, Sako Y, Sho M, Momohara M, Suico MA, Shuto T, Nishitoh H, Okiyoneda T, Kokame K, Kaneko M, Taura M, Miyata M, Chosa K, Koga T, Morino-Koga S, Wada I, Kai H
Molecular cell47(1)99-110(2012 Jul)

Defining the membrane proteome of NK cells.
Ghosh D, Lippert D, Krokhin O, Cortens JP, Wilkins JA
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)

Cotranslational and posttranslational N-glycosylation of polypeptides by distinct mammalian OST isoforms.
Ruiz-Canada C, Kelleher DJ, Gilmore R
Cell136(2)272-83(2009 Jan)

Oligosaccharyltransferase isoforms that contain different catalytic STT3 subunits have distinct enzymatic properties.
Kelleher DJ, Karaoglu D, Mandon EC, Gilmore R
Molecular cell12(1)101-11(2003 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Stt3b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Stt3b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa06298	NM_001170539.1 Latest version!	Rattus norvegicus STT3 oligosaccharyltransferase complex catalytic subunit B (Stt3b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Stt3b ( NM_001170539.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001164010.1 Rattus norvegicus STT3 oligosaccharyltransferase complex catalytic subunit B (Stt3b), mRNA.

Price & Availability↑

CloneID	ORa06298
Clone ID Related Accession (Same CDS sequence)	NM_001170539.1
Accession Version	NM_001170539.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2475bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-18
Organism	Rattus norvegicus(Norway rat)
Product	dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC166740.1. On or before May 22, 2010 this sequence version replaced XM_002727142.1, XM_002729974.2, XR_005437.1, XR_009639.1. ##Evidence-Data-START## Transcript exon combination :: BC166740.1, FQ210494.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGAGC CCTCGGCCCC GGAGAGCAAG CACAAGTCGT CTCTCAACTC GTCCCCGTGG 
AGCGGCCTCA TGGCTCTGGG GAACAGCCGC CACGGGCACC ACGGGCCCGG AGCCCAGAGC 
GCTTCCAGGG CGGCGGCGGC GCCGAAGCCG GGGCCCCCCG CGGGGCTGTC CGGGGGCTTG 
TCGCAGCCGG CCGGGTGGCA GTCTTTGCTC TCCTTCACCA TCCTCTTCCT GGCCTGGCTG 
GCCGGCTTCA GCTCGCGCCT CTTCGCCGTC ATCCGCTTCG AGAGCATCAT CCACGAGTTC 
GACCCGTGGT TTAACTATAG ATCAACTCAT CATCTTGCAT CTCATGGGTT CTATGAGTTC 
TTAAATTGGT TTGATGAAAG AGCATGGTAC CCACTAGGAA GAATCGTGGG TGGCACCGTT 
TACCCAGGAT TGATGATAAC AGCTGGCCTT ATTCATTGGA TTTTAAATAC ATTGAACATA 
ACAGTTCACA TCAGAGATGT ATGTGTGTTC CTTGCACCAA CTTTTAGCGG CCTTACATCT 
ATATCTACGT TCCTGCTAAC TAGAGAACTT TGGAACCAGG GAGCAGGGCT CTTAGCTGCC 
TGCTTCATTG CTATTGTACC CGGGTACATA TCTCGGTCGG TAGCGGGATC CTTTGATAAC 
GAAGGCATTG CGATTTTCGC ACTTCAGTTC ACTTACTACT TATGGGTGAA GTCTGTGAAA 
ACCGGGTCTG TGTTTTGGAC AATGTGCTGC TGCTTGTCGT ATTTCTACAT GGTCTCTGCG 
TGGGGAGGTT ATGTGTTCAT CATCAACCTC ATCCCTCTCC ATGTGTTTGT GCTGCTCCTG 
ATGCAGAGGT ACAGCAAGAG AGTCTACATA GCATATAGCA CTTTCTACAT TGTGGGTTTA 
ATATTATCCA TGCAGATACC TTTTGTGGGA TTCCAGCCAA TCAGGACAAG CGAGCACATG 
GCAGCTGCAG GTGTCTTTGC GCTGCTGCAG GCTTATGCGT TTCTGCAGTA TCTGAGAGAC 
CGGTTGACAA AGCAGGAGTT CCAGACCCTT TTCTTTTTGG GTGTCTCACT AGCTGCAGGC 
GCTGTGTTCC TTAGTGTCAT CTATCTGACA TACACAGGTT ACATTGCACC ATGGAGTGGC 
AGGTTTTATT CATTATGGGA TACTGGGTAT GCAAAAATAC ACATTCCAAT TATTGCATCA 
GTGTCTGAAC ATCAGCCTAC GACATGGGTG TCTTTCTTCT TTGATCTACA TATTCTTGTG 
TGTACCTTCC CAGCAGGCCT ATGGTTCTGC ATCAAAAATA TCAACGATGA AAGAGTATTT 
GTTGCTCTGT ATGCGATCAG TGCTGTCTAC TTTGCTGGAG TAATGGTACG GCTGATGCTG 
ACTCTGACCC CAGTCGTCTG CATGCTGTCT GCAATTGCCT TCTCCAGTGT TTTTGAGCAC 
TATTTGGGGG ATGACATGAA AAGGGAAAAC CCACCTGTGG AGGACAGCAG TGACGAGGAT 
GACAGAAGAA GCCCAGGAAA CTTGTATGAC AAGGCAGGGA AAGTGAGAAA GCATGTGACG 
GAGCAGGAGA AGCCGGAGGA GGGCTTGGGC CCCAACATCA AGAGCATCGT GACCATGCTC 
ATGCTCATGC TCCTCATGAT GTTCGCTGTG CACTGCACCT GGGTCACCAG CAATGCCTAC 
TCCAGCCCCA GTGTGGTCCT CGCCTCCTAC AATCATGATG GAACCAGAAA TATCTTAGAT 
GATTTTAGAG AAGCATACTT TTGGTTGAGA CAAAACACGG ATGAACACGC CAGGGTCATG 
TCCTGGTGGG ACTATGGCTA TCAGATTGCT GGGATGGCCA ACAGGACTAC TCTGGTAGAC 
AATAACACCT GGAACAACAG CCACATTGCA CTGGTAGGAA AAGCTATGTC TTCTAATGAA 
ACAGCCGCCT ATAAAATCAT GAGGTCTCTT GACGTTGATT ATGTGTTGGT CATTTTTGGA 
GGAGTTATTG GCTATTCTGG TGATGATATC AACAAGTTCC TCTGGATGGT CAGGATAGCT 
GAAGGGGAGC ACCCAAAAGA CATTCGGGAA GGCGACTACT TCACCCAGCA GGGAGAGTTC 
CGAGTGGACA GAGCCGGGTC TCCTACTCTG TTAAATTGCC TTATGTATAA AATGTCATAC 
TACAGATTTG GAGAAATGCA GCTAGATTTT CGCACACCCC CCGGTTTTGA CCGAACACGT 
AATGCTGAGA TCGGAAATAA GGACATTAAA TTCAAGCATT TGGAGGAAGC CTTTACATCA 
GAGCACTGGC TTGTCAGGAT ATATAAAGTG AAAGCACCCG ACAACAGGGA GACATTAGGT 
CACAAGCCTC GAGTCACCAA CATCTTCCCC AAACAGAAGT ATTTGTCAAA GAAGACTACT 
AAAAGGAAGC GTGGCTACGT TAAAAATAAG CTAGTTTTTA AGAAAGGCAA GAAAATGTCT 
AAGAAGACTG TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001164010.1
CDS	65..2539
Translation	MAEPSAPESKHKSSLNSSPWSGLMALGNSRHGHHGPGAQSASRAAAAPKPGPPAGLSGGLSQPAGWQSLLSFTILFLAWLAGFSSRLFAVIRFESIIHEFDPWFNYRSTHHLASHGFYEFLNWFDERAWYPLGRIVGGTVYPGLMITAGLIHWILNTLNITVHIRDVCVFLAPTFSGLTSISTFLLTRELWNQGAGLLAACFIAIVPGYISRSVAGSFDNEGIAIFALQFTYYLWVKSVKTGSVFWTMCCCLSYFYMVSAWGGYVFIINLIPLHVFVLLLMQRYSKRVYIAYSTFYIVGLILSMQIPFVGFQPIRTSEHMAAAGVFALLQAYAFLQYLRDRLTKQEFQTLFFLGVSLAAGAVFLSVIYLTYTGYIAPWSGRFYSLWDTGYAKIHIPIIASVSEHQPTTWVSFFFDLHILVCTFPAGLWFCIKNINDERVFVALYAISAVYFAGVMVRLMLTLTPVVCMLSAIAFSSVFEHYLGDDMKRENPPVEDSSDEDDRRSPGNLYDKAGKVRKHVTEQEKPEEGLGPNIKSIVTMLMLMLLMMFAVHCTWVTSNAYSSPSVVLASYNHDGTRNILDDFREAYFWLRQNTDEHARVMSWWDYGYQIAGMANRTTLVDNNTWNNSHIALVGKAMSSNETAAYKIMRSLDVDYVLVIFGGVIGYSGDDINKFLWMVRIAEGEHPKDIREGDYFTQQGEFRVDRAGSPTLLNCLMYKMSYYRFGEMQLDFRTPPGFDRTRNAEIGNKDIKFKHLEEAFTSEHWLVRIYKVKAPDNRETLGHKPRVTNIFPKQKYLSKKTTKRKRGYVKNKLVFKKGKKMSKKTV

Target ORF information:

RefSeq Version	NM_001170539.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus STT3 oligosaccharyltransferase complex catalytic subunit B (Stt3b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001170539.1

ORF Insert Sequence:

ATGGCGGAGC CCTCGGCCCC GGAGAGCAAG CACAAGTCGT CTCTCAACTC GTCCCCGTGG 
AGCGGCCTCA TGGCTCTGGG GAACAGCCGC CACGGGCACC ACGGGCCCGG AGCCCAGAGC 
GCTTCCAGGG CGGCGGCGGC GCCGAAGCCG GGGCCCCCCG CGGGGCTGTC CGGGGGCTTG 
TCGCAGCCGG CCGGGTGGCA GTCTTTGCTC TCCTTCACCA TCCTCTTCCT GGCCTGGCTG 
GCCGGCTTCA GCTCGCGCCT CTTCGCCGTC ATCCGCTTCG AGAGCATCAT CCACGAGTTC 
GACCCGTGGT TTAACTATAG ATCAACTCAT CATCTTGCAT CTCATGGGTT CTATGAGTTC 
TTAAATTGGT TTGATGAAAG AGCATGGTAC CCACTAGGAA GAATCGTGGG TGGCACCGTT 
TACCCAGGAT TGATGATAAC AGCTGGCCTT ATTCATTGGA TTTTAAATAC ATTGAACATA 
ACAGTTCACA TCAGAGATGT ATGTGTGTTC CTTGCACCAA CTTTTAGCGG CCTTACATCT 
ATATCTACGT TCCTGCTAAC TAGAGAACTT TGGAACCAGG GAGCAGGGCT CTTAGCTGCC 
TGCTTCATTG CTATTGTACC CGGGTACATA TCTCGGTCGG TAGCGGGATC CTTTGATAAC 
GAAGGCATTG CGATTTTCGC ACTTCAGTTC ACTTACTACT TATGGGTGAA GTCTGTGAAA 
ACCGGGTCTG TGTTTTGGAC AATGTGCTGC TGCTTGTCGT ATTTCTACAT GGTCTCTGCG 
TGGGGAGGTT ATGTGTTCAT CATCAACCTC ATCCCTCTCC ATGTGTTTGT GCTGCTCCTG 
ATGCAGAGGT ACAGCAAGAG AGTCTACATA GCATATAGCA CTTTCTACAT TGTGGGTTTA 
ATATTATCCA TGCAGATACC TTTTGTGGGA TTCCAGCCAA TCAGGACAAG CGAGCACATG 
GCAGCTGCAG GTGTCTTTGC GCTGCTGCAG GCTTATGCGT TTCTGCAGTA TCTGAGAGAC 
CGGTTGACAA AGCAGGAGTT CCAGACCCTT TTCTTTTTGG GTGTCTCACT AGCTGCAGGC 
GCTGTGTTCC TTAGTGTCAT CTATCTGACA TACACAGGTT ACATTGCACC ATGGAGTGGC 
AGGTTTTATT CATTATGGGA TACTGGGTAT GCAAAAATAC ACATTCCAAT TATTGCATCA 
GTGTCTGAAC ATCAGCCTAC GACATGGGTG TCTTTCTTCT TTGATCTACA TATTCTTGTG 
TGTACCTTCC CAGCAGGCCT ATGGTTCTGC ATCAAAAATA TCAACGATGA AAGAGTATTT 
GTTGCTCTGT ATGCGATCAG TGCTGTCTAC TTTGCTGGAG TAATGGTACG GCTGATGCTG 
ACTCTGACCC CAGTCGTCTG CATGCTGTCT GCAATTGCCT TCTCCAGTGT TTTTGAGCAC 
TATTTGGGGG ATGACATGAA AAGGGAAAAC CCACCTGTGG AGGACAGCAG TGACGAGGAT 
GACAGAAGAA GCCCAGGAAA CTTGTATGAC AAGGCAGGGA AAGTGAGAAA GCATGTGACG 
GAGCAGGAGA AGCCGGAGGA GGGCTTGGGC CCCAACATCA AGAGCATCGT GACCATGCTC 
ATGCTCATGC TCCTCATGAT GTTCGCTGTG CACTGCACCT GGGTCACCAG CAATGCCTAC 
TCCAGCCCCA GTGTGGTCCT CGCCTCCTAC AATCATGATG GAACCAGAAA TATCTTAGAT 
GATTTTAGAG AAGCATACTT TTGGTTGAGA CAAAACACGG ATGAACACGC CAGGGTCATG 
TCCTGGTGGG ACTATGGCTA TCAGATTGCT GGGATGGCCA ACAGGACTAC TCTGGTAGAC 
AATAACACCT GGAACAACAG CCACATTGCA CTGGTAGGAA AAGCTATGTC TTCTAATGAA 
ACAGCCGCCT ATAAAATCAT GAGGTCTCTT GACGTTGATT ATGTGTTGGT CATTTTTGGA 
GGAGTTATTG GCTATTCTGG TGATGATATC AACAAGTTCC TCTGGATGGT CAGGATAGCT 
GAAGGGGAGC ACCCAAAAGA CATTCGGGAA GGCGACTACT TCACCCAGCA GGGAGAGTTC 
CGAGTGGACA GAGCCGGGTC TCCTACTCTG TTAAATTGCC TTATGTATAA AATGTCATAC 
TACAGATTTG GAGAAATGCA GCTAGATTTT CGCACACCCC CCGGTTTTGA CCGAACACGT 
AATGCTGAGA TCGGAAATAA GGACATTAAA TTCAAGCATT TGGAGGAAGC CTTTACATCA 
GAGCACTGGC TTGTCAGGAT ATATAAAGTG AAAGCACCCG ACAACAGGGA GACATTAGGT 
CACAAGCCTC GAGTCACCAA CATCTTCCCC AAACAGAAGT ATTTGTCAAA GAAGACTACT 
AAAAGGAAGC GTGGCTACGT TAAAAATAAG CTAGTTTTTA AGAAAGGCAA GAAAATGTCT 
AAGAAGACTG TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

ZMPSTE24 defends against influenza and other pathogenic viruses.
The Journal of experimental medicine214(4)919-929(2017 Apr)
Fu B,Wang L,Li S,Dorf ME

STT3B-dependent posttranslational N-glycosylation as a surveillance system for secretory protein.
Molecular cell47(1)99-110(2012 Jul)
Sato T,Sako Y,Sho M,Momohara M,Suico MA,Shuto T,Nishitoh H,Okiyoneda T,Kokame K,Kaneko M,Taura M,Miyata M,Chosa K,Koga T,Morino-Koga S,Wada I,Kai H

Defining the membrane proteome of NK cells.
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)
Ghosh D,Lippert D,Krokhin O,Cortens JP,Wilkins JA

Cotranslational and posttranslational N-glycosylation of polypeptides by distinct mammalian OST isoforms.
Cell136(2)272-83(2009 Jan)
Ruiz-Canada C,Kelleher DJ,Gilmore R

Oligosaccharyltransferase isoforms that contain different catalytic STT3 subunits have distinct enzymatic properties.
Molecular cell12(1)101-11(2003 Jul)
Kelleher DJ,Karaoglu D,Mandon EC,Gilmore R