Siva1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Siva1
Entrez Gene ID	362791
Full Name	SIVA1, apoptosis-inducing factor
Synonyms	Siva
General protein information	Preferred Names SIVA1, apoptosis-inducing factor Names apoptosis regulatory protein Siva CD27-binding protein CD27BP
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 6 Map Location: 6q32

mRNA and Protein(s)

mRNA	Protein	Name
XM_006240645.3	XP_006240707.1	apoptosis regulatory protein Siva isoform X1
NM_001100982.1	NP_001094452.1	apoptosis regulatory protein Siva

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Siva1	NP_038957.2
Gallus gallus (chicken)	SIVA1	NP_001264464.1
Bos taurus (cattle)	SIVA1	NP_001180107.1
Rattus norvegicus (Norway rat)	Siva1	NP_001094452.1
Danio rerio (zebrafish)	siva1	XP_700568.6
Canis lupus familiaris (dog)	SIVA1	XP_855513.1
Homo sapiens (human)	SIVA1	NP_006418.2
Pan troglodytes (chimpanzee)	SIVA1	XP_510197.3
	SIVA1	XP_001095069.2
Xenopus tropicalis (tropical clawed frog)	siva1	NP_001039138.1

Gene Ontology
Bibliography

Related articles in PubMed

Expression of apoptosis-inducing factor (AIF) in the aged rat brain.
Yu W, Gubkina O, Mechawar N, Elwell D, Quirion R, Krantic S
Neurobiology of aging32(1)179-80(2011 Jan)

The zinc containing pro-apoptotic protein siva interacts with the peroxisomal membrane protein pmp22.
Nestler M, Martin U, Hortschansky P, Saluz HP, Henke A, Munder T
Molecular and cellular biochemistry287(1-2)147-55(2006 Jul)

Siva-1 negatively regulates NF-kappaB activity: effect on T-cell receptor-mediated activation-induced cell death (AICD).
Gudi R, Barkinge J, Hawkins S, Chu F, Manicassamy S, Sun Z, Duke-Cohan JS, Prasad KV
Oncogene25(24)3458-62(2006 Jun)

Siva-1 and an alternative splice form lacking the death domain, Siva-2, similarly induce apoptosis in T lymphocytes via a caspase-dependent mitochondrial pathway.
Py B, Slomianny C, Auberger P, Petit PX, Benichou S
Journal of immunology (Baltimore, Md. : 1950)172(7)4008-17(2004 Apr)

The Siva-1 putative amphipathic helical region (SAH) is sufficient to bind to BCL-XL and sensitize cells to UV radiation induced apoptosis.
Chu F, Borthakur A, Sun X, Barkinge J, Gudi R, Hawkins S, Prasad KV
Apoptosis : an international journal on programmed cell death9(1)83-95(2004 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This study demonistrated that AIF more abundantly expressed in striatum than in the other two brain regions.
Title: Expression of apoptosis-inducing factor (AIF) in the aged rat brain.

The following Siva1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Siva1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa00461	NM_001100982.1 Latest version!	Rattus norvegicus SIVA1, apoptosis-inducing factor (Siva1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
ORa29675	XM_006240645.3 Latest version!	Rattus norvegicus SIVA1, apoptosis-inducing factor (Siva1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Siva1 ( NM_001100982.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001094452.1 Rattus norvegicus SIVA1, apoptosis-inducing factor (Siva1), mRNA.

Price & Availability↑

CloneID	ORa00461
Clone ID Related Accession (Same CDS sequence)	NM_001100982.1
Accession Version	NM_001100982.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 474bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Rattus norvegicus(Norway rat)
Product	apoptosis regulatory protein Siva
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC088866.1. On or before Aug 13, 2007 this sequence version replaced XM_343117.3, XM_001072396.1, XM_001072975.1. ##Evidence-Data-START## Transcript exon combination :: BC088866.1, CB775586.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGCCCAAGC GGAGCTGCCC GTTCACGGAT GCTGCCCCGC TTCAACTCAA AGTCCACGTG 
AGCCCGAGAG AGCTGAGCCA CGGTGTGTTC GCCGAGCGCT ACTCGCGCGA GGTCTTCGAA 
AGAACCAAGC AGCTCCTTTT CCAAGGGGCC CAGGCCTATA GAGATCACAT CTGGGGTGAA 
GGTTGTTCCA TCAACCACCT GCCGGAGCCA CTCAAGCCTG GTCTGGTGGG AGCCCCTCAA 
GCTGCAAGGG GACAGATGTT GATTGGACCT GATGGCCGAC TGACACGGTG CCAAGCTCAG 
GCCTCGGAAG CTGGCCTTCC CGGGGCAGCA CCCATTGCTT GTTCATCCTG CGTGAGATCT 
GTGGATGGAA AGGCGGTCTG CAGCCAGTGT GAGCGGGCCC TGTGTGGGCA GTGTGTGTAC 
ACCTGCTGGG GCTGTGGTGC CTTGGCCTGT GTGCTGTGTG GCCTGGCAGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001094452.1
CDS	13..486
Translation	MPKRSCPFTDAAPLQLKVHVSPRELSHGVFAERYSREVFERTKQLLFQGAQAYRDHIWGEGCSINHLPEPLKPGLVGAPQAARGQMLIGPDGRLTRCQAQASEAGLPGAAPIACSSCVRSVDGKAVCSQCERALCGQCVYTCWGCGALACVLCGLAE

Target ORF information:

RefSeq Version	NM_001100982.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus SIVA1, apoptosis-inducing factor (Siva1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001100982.1

ORF Insert Sequence:

ATGCCCAAGC GGAGCTGCCC GTTCACGGAT GCTGCCCCGC TTCAACTCAA AGTCCACGTG 
AGCCCGAGAG AGCTGAGCCA CGGTGTGTTC GCCGAGCGCT ACTCGCGCGA GGTCTTCGAA 
AGAACCAAGC AGCTCCTTTT CCAAGGGGCC CAGGCCTATA GAGATCACAT CTGGGGTGAA 
GGTTGTTCCA TCAACCACCT GCCGGAGCCA CTCAAGCCTG GTCTGGTGGG AGCCCCTCAA 
GCTGCAAGGG GACAGATGTT GATTGGACCT GATGGCCGAC TGACACGGTG CCAAGCTCAG 
GCCTCGGAAG CTGGCCTTCC CGGGGCAGCA CCCATTGCTT GTTCATCCTG CGTGAGATCT 
GTGGATGGAA AGGCGGTCTG CAGCCAGTGT GAGCGGGCCC TGTGTGGGCA GTGTGTGTAC 
ACCTGCTGGG GCTGTGGTGC CTTGGCCTGT GTGCTGTGTG GCCTGGCAGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Siva1 ( XM_006240645.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006240707.1 Rattus norvegicus SIVA1, apoptosis-inducing factor (Siva1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa29675
Clone ID Related Accession (Same CDS sequence)	XM_006240645.3
Accession Version	XM_006240645.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 534bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	apoptosis regulatory protein Siva isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005105.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006240645.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCAAGC GGAGCTGCCC GTTCACGGAT GCTGCCCCGC TTCAACTCAA AGTCCACGTG 
AGCCCGAGAG AGCTGAGCCA CGGTGTGTTC GCCGAGCGCT ACTCGCGCGA GGTCTTCGAA 
AGAACCAAGC AGCTCCTTTT CCAAGGGGCC CAGGCCTATA GAGATCACAT CTGGGGTGAA 
GGTTGTTCCA TCAACCACCT GCCGGAGCCA CTCAAGCCTG GTCTGGTGGG AGCCCCTCAA 
GCTGCAAGGG GACAGATGTT GATTGGACCT GATGGCCGAC TGACACGGTG CCAAGCTCAG 
GCCTCGGAAG CTGGCCTTCC CGGGGCAGCA CCCATTGCTT GTTCATCCTG CGTGAGATCT 
GTGGATGGAA AGGCGGTCTG CAGCCAGTGT GAGCGGGCCC TGTGTGGGCA GTGTGTGTAC 
ACCTGCTGGG GCTGTGGTGC CTTGGCCTGT GTGCTGTGTG GCCTGGCAGA CTATGCCGAC 
GATGACGATG GTGAAAAGAC GCTTTGCACC AGCTGCGCCA TGTTTGAAGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006240707.1
CDS	135..668
Translation	MPKRSCPFTDAAPLQLKVHVSPRELSHGVFAERYSREVFERTKQLLFQGAQAYRDHIWGEGCSINHLPEPLKPGLVGAPQAARGQMLIGPDGRLTRCQAQASEAGLPGAAPIACSSCVRSVDGKAVCSQCERALCGQCVYTCWGCGALACVLCGLADYADDDDGEKTLCTSCAMFEA

Target ORF information:

RefSeq Version	XM_006240645.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus SIVA1, apoptosis-inducing factor (Siva1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006240645.3

ORF Insert Sequence:

ATGCCCAAGC GGAGCTGCCC GTTCACGGAT GCTGCCCCGC TTCAACTCAA AGTCCACGTG 
AGCCCGAGAG AGCTGAGCCA CGGTGTGTTC GCCGAGCGCT ACTCGCGCGA GGTCTTCGAA 
AGAACCAAGC AGCTCCTTTT CCAAGGGGCC CAGGCCTATA GAGATCACAT CTGGGGTGAA 
GGTTGTTCCA TCAACCACCT GCCGGAGCCA CTCAAGCCTG GTCTGGTGGG AGCCCCTCAA 
GCTGCAAGGG GACAGATGTT GATTGGACCT GATGGCCGAC TGACACGGTG CCAAGCTCAG 
GCCTCGGAAG CTGGCCTTCC CGGGGCAGCA CCCATTGCTT GTTCATCCTG CGTGAGATCT 
GTGGATGGAA AGGCGGTCTG CAGCCAGTGT GAGCGGGCCC TGTGTGGGCA GTGTGTGTAC 
ACCTGCTGGG GCTGTGGTGC CTTGGCCTGT GTGCTGTGTG GCCTGGCAGA CTATGCCGAC 
GATGACGATG GTGAAAAGAC GCTTTGCACC AGCTGCGCCA TGTTTGAAGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Expression of apoptosis-inducing factor (AIF) in the aged rat brain.
Neurobiology of aging32(1)179-80(2011 Jan)
Yu W,Gubkina O,Mechawar N,Elwell D,Quirion R,Krantic S

The zinc containing pro-apoptotic protein siva interacts with the peroxisomal membrane protein pmp22.
Molecular and cellular biochemistry287(1-2)147-55(2006 Jul)
Nestler M,Martin U,Hortschansky P,Saluz HP,Henke A,Munder T

Siva-1 negatively regulates NF-kappaB activity: effect on T-cell receptor-mediated activation-induced cell death (AICD).
Oncogene25(24)3458-62(2006 Jun)
Gudi R,Barkinge J,Hawkins S,Chu F,Manicassamy S,Sun Z,Duke-Cohan JS,Prasad KV

Siva-1 and an alternative splice form lacking the death domain, Siva-2, similarly induce apoptosis in T lymphocytes via a caspase-dependent mitochondrial pathway.
Journal of immunology (Baltimore, Md. : 1950)172(7)4008-17(2004 Apr)
Py B,Slomianny C,Auberger P,Petit PX,Benichou S

The Siva-1 putative amphipathic helical region (SAH) is sufficient to bind to BCL-XL and sensitize cells to UV radiation induced apoptosis.
Apoptosis : an international journal on programmed cell death9(1)83-95(2004 Jan)
Chu F,Borthakur A,Sun X,Barkinge J,Gudi R,Hawkins S,Prasad KV