Ndufaf4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ndufaf4
Entrez Gene ID	362495
Full Name	NADH:ubiquinone oxidoreductase complex assembly factor 4
Synonyms	Hrpap20
General protein information	Preferred Names NADH:ubiquinone oxidoreductase complex assembly factor 4 Names NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 4 NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, assembly factor 4 NADH dehydrogenase (ubiquinone) complex I, assembly factor 4 hormone-regulated proliferation associated protein 20 hormone-regulated proliferation-associated 20 kDa protein hormone-regulated proliferation-associated protein of 20 kDa homolog
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 5 Map Location: 5q21
Summary	plays a role in positive regulation of cell proliferation and cell survival [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_198783.1	NP_942078.1	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 4

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Ndufaf4	NP_942078.1
Drosophila melanogaster (fruit fly)	CG11722	NP_649970.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007625	XP_308247.4
Canis lupus familiaris (dog)	NDUFAF4	XP_532238.1
Bos taurus (cattle)	NDUFAF4	NP_001076985.1
Xenopus tropicalis (tropical clawed frog)	ndufaf4	NP_001135673.1
Pan troglodytes (chimpanzee)	NDUFAF4	XP_001135738.1
Mus musculus (house mouse)	Ndufaf4	NP_081018.1
Danio rerio (zebrafish)	ndufaf4	XP_003200703.1
Homo sapiens (human)	NDUFAF4	NP_054884.1
	NDUFAF4	XP_001100962.1
Gallus gallus (chicken)	NDUFAF4	XP_003641061.1

Gene Ontology
Bibliography

Related articles in PubMed

The mitochondrial disease associated protein Ndufaf2 is dispensable for Complex-1 assembly but critical for the regulation of oxidative stress.
Schlehe JS, Journel MS, Taylor KP, Amodeo KD, LaVoie MJ
Neurobiology of disease5857-67(2013 Oct)

Mutations in NDUFAF3 (C3ORF60), encoding an NDUFAF4 (C6ORF66)-interacting complex I assembly protein, cause fatal neonatal mitochondrial disease.
Saada A, Vogel RO, Hoefs SJ, van den Brand MA, Wessels HJ, Willems PH, Venselaar H, Shaag A, Barghuti F, Reish O, Shohat M, Huynen MA, Smeitink JA, van den Heuvel LP, Nijtmans LG
American journal of human genetics84(6)718-27(2009 Jun)

C6ORF66 is an assembly factor of mitochondrial complex I.
Saada A, Edvardson S, Rapoport M, Shaag A, Amry K, Miller C, Lorberboum-Galski H, Elpeleg O
American journal of human genetics82(1)32-8(2008 Jan)

Identification of HRPAP20: a novel phosphoprotein that enhances growth and survival in hormone-responsive tumor cells.
Karp CM, Pan H, Zhang M, Buckley DJ, Schuler LA, Buckley AR
Cancer research64(3)1016-25(2004 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Ndufaf4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ndufaf4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa13067	NM_198783.1 Latest version!	Rattus norvegicus NADH:ubiquinone oxidoreductase complex assembly factor 4 (Ndufaf4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ndufaf4 ( NM_198783.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_942078.1 Rattus norvegicus NADH:ubiquinone oxidoreductase complex assembly factor 4 (Ndufaf4), mRNA.

Price & Availability↑

CloneID	ORa13067
Clone ID Related Accession (Same CDS sequence)	NM_198783.1
Accession Version	NM_198783.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 525bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Rattus norvegicus(Norway rat)
Product	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 4
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF283900.1. On Dec 17, 2003 this sequence version replaced XM_342813.1. ##Evidence-Data-START## Transcript exon combination :: AF283900.1, EV776950.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGGGGGCTC GCATGACCCG CGCCTTCAGG AACTTCAACG TGGAGAAGCG AGCGGAGCAG 
GAGATCAGCA AGAGGAAGCC CTCCATGGCA CCCAAGCACC CTTCCACCCG CAGTCTCCTG 
CAGGAGCACC TGAGTCAGTA TCCAGAAATC GAGGAAGAAG TTTCTAGAAA AGATAACAAG 
CTGCTGTCCT TACTAAGAGA TGTATATGTC AATTCCAAAG ATCCGGTGCC TTCCTTGCCG 
GTAAAAGCCA TTGAACCACA GCAACAACCA AAGGAGTTCA GATTGCCAAT AGGAGATCAA 
TTTGACAAGA ATATCAAGGA CATTCCCAAA GGCAAGATCA CTGTTGTAGA AGCACTGACC 
CTTCTCAATA ACCATAAACT CTCTCCAGAG ACATGGACTG CTGAGAAAAT CGCCCAGGAA 
TACCATTTAG AACTGGAGGA TGTAAATTCC CTTCTCAAAT ATTTCGTTAC TTTTGAAGTC 
AAAATCGTTC CTCCTGAAGA CAGGAAAGCA ATACAATCAA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_942078.1
CDS	38..562
Translation	MGARMTRAFRNFNVEKRAEQEISKRKPSMAPKHPSTRSLLQEHLSQYPEIEEEVSRKDNKLLSLLRDVYVNSKDPVPSLPVKAIEPQQQPKEFRLPIGDQFDKNIKDIPKGKITVVEALTLLNNHKLSPETWTAEKIAQEYHLELEDVNSLLKYFVTFEVKIVPPEDRKAIQSK

Target ORF information:

RefSeq Version	NM_198783.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus NADH:ubiquinone oxidoreductase complex assembly factor 4 (Ndufaf4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_198783.1

ORF Insert Sequence:

ATGGGGGCTC GCATGACCCG CGCCTTCAGG AACTTCAACG TGGAGAAGCG AGCGGAGCAG 
GAGATCAGCA AGAGGAAGCC CTCCATGGCA CCCAAGCACC CTTCCACCCG CAGTCTCCTG 
CAGGAGCACC TGAGTCAGTA TCCAGAAATC GAGGAAGAAG TTTCTAGAAA AGATAACAAG 
CTGCTGTCCT TACTAAGAGA TGTATATGTC AATTCCAAAG ATCCGGTGCC TTCCTTGCCG 
GTAAAAGCCA TTGAACCACA GCAACAACCA AAGGAGTTCA GATTGCCAAT AGGAGATCAA 
TTTGACAAGA ATATCAAGGA CATTCCCAAA GGCAAGATCA CTGTTGTAGA AGCACTGACC 
CTTCTCAATA ACCATAAACT CTCTCCAGAG ACATGGACTG CTGAGAAAAT CGCCCAGGAA 
TACCATTTAG AACTGGAGGA TGTAAATTCC CTTCTCAAAT ATTTCGTTAC TTTTGAAGTC 
AAAATCGTTC CTCCTGAAGA CAGGAAAGCA ATACAATCAA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The mitochondrial disease associated protein Ndufaf2 is dispensable for Complex-1 assembly but critical for the regulation of oxidative stress.
Neurobiology of disease5857-67(2013 Oct)
Schlehe JS,Journel MS,Taylor KP,Amodeo KD,LaVoie MJ

Mutations in NDUFAF3 (C3ORF60), encoding an NDUFAF4 (C6ORF66)-interacting complex I assembly protein, cause fatal neonatal mitochondrial disease.
American journal of human genetics84(6)718-27(2009 Jun)
Saada A,Vogel RO,Hoefs SJ,van den Brand MA,Wessels HJ,Willems PH,Venselaar H,Shaag A,Barghuti F,Reish O,Shohat M,Huynen MA,Smeitink JA,van den Heuvel LP,Nijtmans LG

C6ORF66 is an assembly factor of mitochondrial complex I.
American journal of human genetics82(1)32-8(2008 Jan)
Saada A,Edvardson S,Rapoport M,Shaag A,Amry K,Miller C,Lorberboum-Galski H,Elpeleg O

Identification of HRPAP20: a novel phosphoprotein that enhances growth and survival in hormone-responsive tumor cells.
Cancer research64(3)1016-25(2004 Feb)
Karp CM,Pan H,Zhang M,Buckley DJ,Schuler LA,Buckley AR