Ube2m cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ube2m
Entrez Gene ID	361509
Full Name	ubiquitin-conjugating enzyme E2M
General protein information	Preferred Names ubiquitin-conjugating enzyme E2M Names NEDD8-conjugating enzyme Ubc12 ubiquitin-conjugating enzyme E2M (UBC12 homolog, yeast)
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q12

mRNA and Protein(s)

mRNA	Protein	Name
NM_001108471.1	NP_001101941.1	NEDD8-conjugating enzyme Ubc12

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	UBE2M	XP_001146573.2
	UBE2M	XP_001099932.1
Bos taurus (cattle)	UBE2M	NP_001076972.1
Schizosaccharomyces pombe (fission yeast)	ubc12	NP_588256.1
Mus musculus (house mouse)	Ube2m	NP_663553.1
Magnaporthe oryzae (rice blast fungus)	MGG_01807	XP_003714818.1
Neurospora crassa	NCU03610	XP_961337.1
Oryza sativa (rice)	Os09g0321900	NP_001062859.1
Homo sapiens (human)	UBE2M	NP_003960.1
Canis lupus familiaris (dog)	UBE2M	XP_541337.3
Drosophila melanogaster (fruit fly)	Ubc12	NP_001261568.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005916	XP_315947.2
Saccharomyces cerevisiae (baker's yeast)	UBC12	NP_013409.1
Arabidopsis thaliana (thale cress)	RCE1	NP_001154289.1
Arabidopsis thaliana (thale cress)	AT2G18600	NP_565440.1
Rattus norvegicus (Norway rat)	Ube2m	NP_001101941.1
Danio rerio (zebrafish)	wu:fb58e05	XP_001920670.3
Kluyveromyces lactis	KLLA0C17248g	XP_452971.1
Oryza sativa (rice)	Os08g0374100	NP_001061664.1
Eremothecium gossypii	AGOS_ADL035C	NP_984062.1
Xenopus tropicalis (tropical clawed frog)	ube2m	NP_988956.1

Gene Ontology
Bibliography

Related articles in PubMed

E2-RING expansion of the NEDD8 cascade confers specificity to cullin modification.
Huang DT, Ayrault O, Hunt HW, Taherbhoy AM, Duda DM, Scott DC, Borg LA, Neale G, Murray PJ, Roussel MF, Schulman BA
Molecular cell33(4)483-95(2009 Feb)

Structural basis for recruitment of Ubc12 by an E2 binding domain in NEDD8's E1.
Huang DT, Paydar A, Zhuang M, Waddell MB, Holton JM, Schulman BA
Molecular cell17(3)341-50(2005 Feb)

A unique E1-E2 interaction required for optimal conjugation of the ubiquitin-like protein NEDD8.
Huang DT, Miller DW, Mathew R, Cassell R, Holton JM, Roussel MF, Schulman BA
Nature structural & molecular biology11(10)927-35(2004 Oct)

APP-BP1 mediates APP-induced apoptosis and DNA synthesis and is increased in Alzheimer's disease brain.
Chen Y, Liu W, McPhie DL, Hassinger L, Neve RL
The Journal of cell biology163(1)27-33(2003 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Ube2m gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ube2m cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa00361	NM_001108471.1 Latest version!	Rattus norvegicus ubiquitin-conjugating enzyme E2M (Ube2m), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

ORF Online Only Promotion

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5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ube2m ( NM_001108471.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001101941.1 Rattus norvegicus ubiquitin-conjugating enzyme E2M (Ube2m), mRNA.

Price & Availability↑

CloneID	ORa00361
Clone ID Related Accession (Same CDS sequence)	NM_001108471.1
Accession Version	NM_001108471.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 552bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-27
Organism	Rattus norvegicus(Norway rat)
Product	NEDD8-conjugating enzyme Ubc12
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH474090.1. On or before Oct 4, 2007 this sequence version replaced XM_341790.3, XM_001062179.1. ##Evidence-Data-START## Transcript exon combination :: CV118842.1, CO556027.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGATCAAGC TGTTCTCGCT GAAGCAGCAG AAGAAGGAGG AGGAGTCGGC CGGCGGCACC 
AAGGGCAGCA GCAAGAAGGC GTCGGCGGCG CAGCTGCGGA TTCAGAAGGA CATTAACGAG 
CTGAACCTGC CCAAGACGTG TGACATCAGC TTCTCAGACC CAGACGACCT CCTCAACTTC 
AAACTGGTTA TCTGTCCTGA TGAAGGCTTC TACAAGAGCG GCAAGTTTGT GTTCAGCTTT 
AAGGTGGGAC AGGGTTACCC ACATGACCCT CCCAAGGTGA AGTGTGAAAC AATGGTTTAT 
CATCCCAACA TTGACCTCGA GGGCAACGTC TGCCTCAACA TCCTCAGAGA GGACTGGAAG 
CCAGTCCTTA CGATAAACTC CATAATTTAT GGCCTGCAGT ATCTCTTCTT GGAGCCCAAC 
CCCGAGGACC CACTGAACAA GGAGGCTGCC GAGGTCCTGC AGAACAATCG GCGGCTGTTT 
GAACAAAACG TGCAGCGCTC CATGAGAGGT GGTTACATCG GGTCCACCTA CTTCGAGCGC 
TGCCTGAAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001101941.1
CDS	574..1125
Translation	MIKLFSLKQQKKEEESAGGTKGSSKKASAAQLRIQKDINELNLPKTCDISFSDPDDLLNFKLVICPDEGFYKSGKFVFSFKVGQGYPHDPPKVKCETMVYHPNIDLEGNVCLNILREDWKPVLTINSIIYGLQYLFLEPNPEDPLNKEAAEVLQNNRRLFEQNVQRSMRGGYIGSTYFERCLK

Target ORF information:

RefSeq Version	NM_001108471.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ubiquitin-conjugating enzyme E2M (Ube2m), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001108471.1

ORF Insert Sequence:

ATGATCAAGC TGTTCTCGCT GAAGCAGCAG AAGAAGGAGG AGGAGTCGGC CGGCGGCACC 
AAGGGCAGCA GCAAGAAGGC GTCGGCGGCG CAGCTGCGGA TTCAGAAGGA CATTAACGAG 
CTGAACCTGC CCAAGACGTG TGACATCAGC TTCTCAGACC CAGACGACCT CCTCAACTTC 
AAACTGGTTA TCTGTCCTGA TGAAGGCTTC TACAAGAGCG GCAAGTTTGT GTTCAGCTTT 
AAGGTGGGAC AGGGTTACCC ACATGACCCT CCCAAGGTGA AGTGTGAAAC AATGGTTTAT 
CATCCCAACA TTGACCTCGA GGGCAACGTC TGCCTCAACA TCCTCAGAGA GGACTGGAAG 
CCAGTCCTTA CGATAAACTC CATAATTTAT GGCCTGCAGT ATCTCTTCTT GGAGCCCAAC 
CCCGAGGACC CACTGAACAA GGAGGCTGCC GAGGTCCTGC AGAACAATCG GCGGCTGTTT 
GAACAAAACG TGCAGCGCTC CATGAGAGGT GGTTACATCG GGTCCACCTA CTTCGAGCGC 
TGCCTGAAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

E2-RING expansion of the NEDD8 cascade confers specificity to cullin modification.
Molecular cell33(4)483-95(2009 Feb)
Huang DT,Ayrault O,Hunt HW,Taherbhoy AM,Duda DM,Scott DC,Borg LA,Neale G,Murray PJ,Roussel MF,Schulman BA

Structural basis for recruitment of Ubc12 by an E2 binding domain in NEDD8's E1.
Molecular cell17(3)341-50(2005 Feb)
Huang DT,Paydar A,Zhuang M,Waddell MB,Holton JM,Schulman BA

A unique E1-E2 interaction required for optimal conjugation of the ubiquitin-like protein NEDD8.
Nature structural & molecular biology11(10)927-35(2004 Oct)
Huang DT,Miller DW,Mathew R,Cassell R,Holton JM,Roussel MF,Schulman BA

APP-BP1 mediates APP-induced apoptosis and DNA synthesis and is increased in Alzheimer's disease brain.
The Journal of cell biology163(1)27-33(2003 Oct)
Chen Y,Liu W,McPhie DL,Hassinger L,Neve RL