Top2b cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Top2b
Entrez Gene ID	361100
Full Name	DNA topoisomerase II beta
General protein information	Preferred Names DNA topoisomerase II beta Names DNA topoisomerase 2-beta topoisomerase (DNA) II beta topoisomerase II beta
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 15 Map Location: 15p16
Summary	a nuclear enzyme that catalyzes the breaking and rejoining of two strands of duplex DNA [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001100858.1	NP_001094328.1	DNA topoisomerase 2-beta

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	TOP2	NP_014311.3
Schizosaccharomyces pombe (fission yeast)	top2	NP_595805.1
Oryza sativa (rice)	Os02g0699700	NP_001047833.1
Pan troglodytes (chimpanzee)	TOP2B	XP_516332.3
	TOP2B	XP_001092092.2
Gallus gallus (chicken)	TOP2B	NP_990413.1
Neurospora crassa	NCU06338	XP_962372.2
Danio rerio (zebrafish)	top2b	NP_001038656.1
Bos taurus (cattle)	TOP2B	XP_002698796.2
Mus musculus (house mouse)	Top2b	NP_033435.2
Magnaporthe oryzae (rice blast fungus)	MGG_06162	XP_003712042.1
Arabidopsis thaliana (thale cress)	TOPII	NP_001118686.1
Homo sapiens (human)	TOP2B	NP_001059.2
Canis lupus familiaris (dog)	TOP2B	XP_534241.3
Rattus norvegicus (Norway rat)	Top2b	NP_001094328.1
Kluyveromyces lactis	KLLA0C13838g	XP_452818.1
Eremothecium gossypii	AGOS_AER410W	NP_985265.1
Xenopus tropicalis (tropical clawed frog)	top2b	XP_004915443.1

Gene Ontology
Bibliography

Related articles in PubMed

Anthracyclines/trastuzumab: new aspects of cardiotoxicity and molecular mechanisms.
Rochette L, Guenancia C, Gudjoncik A, Hachet O, Zeller M, Cottin Y, Vergely C
Trends in pharmacological sciences36(6)326-48(2015 Jun)

Nuclear dynamics of topoisomerase II? reflects its catalytic activity that is regulated by binding of RNA to the C-terminal domain.
Onoda A, Hosoya O, Sano K, Kiyama K, Kimura H, Kawano S, Furuta R, Miyaji M, Tsutsui K, Tsutsui KM
Nucleic acids research42(14)9005-20(2014 Aug)

Doxorubicin, DNA torsion, and chromatin dynamics.
Yang F, Teves SS, Kemp CJ, Henikoff S
Biochimica et biophysica acta1845(1)84-9(2014 Jan)

Catalytic inhibitors of topoisomerase II differently modulate the toxicity of anthracyclines in cardiac and cancer cells.
Vavrova A, Jansova H, Mackova E, Machacek M, Haskova P, Tichotova L, Sterba M, Simunek T
PloS one8(10)e76676(2013)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data indicate that dexrazoxane may protect cardiomyocytes via its catalytic topoisomerase II (TOP2) inhibitory activity.
Title: Catalytic inhibitors of topoisomerase II differently modulate the toxicity of anthracyclines in cardiac and cancer cells.

Suggest a role for TopoIIbeta in the repair of N-ethyl N-nitroso urea-mediated strand breaks via base excision repair pathway.
Title: Topoisomerase II? regulates base excision repair capacity of neurons.

TopoIIbeta is an essential component in DSBs repair in primary neurons in both Ku70 and PARP-1 dependent pathways.
Title: Topoisomerase II? associates with Ku70 and PARP-1 during double strand break repair of DNA in neurons.

hnRNP U/SAF-A/SP120 regulates the enzyme DNA Topoisomerase IIbeta in dual ways
Title: Regulation of DNA Topoisomerase IIbeta through RNA-dependent association with heterogeneous nuclear ribonucleoprotein U (hnRNP U).

Topoisomerase IIbeta activates a subset of neuronal genes that are repressed in AT-rich genomic environment
Title: Topoisomerase IIbeta activates a subset of neuronal genes that are repressed in AT-rich genomic environment.

The following Top2b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Top2b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa11711	NM_001100858.1 Latest version!	Rattus norvegicus DNA topoisomerase II beta (Top2b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Top2b ( NM_001100858.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001094328.1 Rattus norvegicus DNA topoisomerase II beta (Top2b), mRNA.

Price & Availability↑

CloneID	ORa11711
Clone ID Related Accession (Same CDS sequence)	NM_001100858.1
Accession Version	NM_001100858.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4845bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-27
Organism	Rattus norvegicus(Norway rat)
Product	DNA topoisomerase 2-beta
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB262979.1. On or before May 13, 2010 this sequence version replaced XM_341386.4, XM_001067768.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB262979.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGCCAAGT CCAGCCTCGC GGGCGTTGAC GGGGCGCTGA CCTGGGTGAA CAATGCTGCA 
AAAAAGGAAG AGTTGGAGAC AGCGAACAAA AATGATTCTT CCAAGAAGTT GTCTGTTGAG 
AGAGTGTACC AGAAGAAGAC CCAACTGGAG CATATTCTCC TTCGTCCTGA TACATACATT 
GGATCAGTGG AACCACTGAC ACAGTTAATG TGGGTGTATG ACGAAGATGT AGGCATGAAC 
TGCAGAGAGG TTACCTTTGT TCCGGGCTTA TACAAGATCT TTGATGAAAT TTTGGTCAAT 
GCTGCTGACA ACAAGCAGAG GGATAAGAAC ATGACCTGTA TTAAGGTTTC TATTGATCCT 
GAATCTAATA TTATAAGCAT TTGGAATAAT GGAAAAGGCA TTCCAGTAGT AGAACACAAA 
GTAGAGAAAG TTTATGTTCC TGCTTTAATT TTTGGACAGC TTTTAACATC GAGTAATTAC 
GATGATGATG AGAAAAAAGT TACAGGAGGC CGTAATGGTT ATGGTGCAAA ACTTTGTAAT 
ATTTTTAGTA CAAAGTTTAC AGTAGAAACG GCTTGCAAAG AATACAAACA TAGTTTTAAG 
CAGACATGGA TGAATAATAT GATGAAGACT TCTGAAGCCA AAATCAAACA TTTTGATGGG 
GAAGATTATA CATGCATAAC ATTCCAGCCA GATCTAGCCA AGTTTAAGAT GGAAAAACTT 
GACAAGGACA TTGTGGCCCT CATGACCAGA AGGGCATACG ATTTAGCTGG TTCCTGCAAA 
GGCGTCAAGG TCATGTTCAA TGGCAAGAAG TTGCCTGTAA ATGGATTTCG CAGCTATGTA 
GACCTTTATG TGAAAGACAA ATTGGATGAA ACTGGGGTCG CTCTCAAAGT TATTCATGAA 
CTTGCAAATG AAAGATGGGA TGTTTGTCTA ACCTTGAGTG AAAAAGGTTT CCAGCAGATC 
AGCTTTGTGA ATAGTATTGC AACTACAAAA GGTGGACGGC ACGTGGATTA CGTAGTAGAT 
CAAGTTGTTG GTAAACTGAT TGAAGTAGTT AAGAAAAAGA ACAAAGCTGG TGTATCAGTG 
AAGCCATTTC AAGTTAAAAA CCACATATGG GTTTTTATTA ATTGTCTTAT TGAAAATCCA 
ACCTTTGATT CTCAAACTAA GGAAAACATG ACTCTGCAGC CCAAGAGCTT TGGGTCTAAA 
TGCCAGCTGT CAGAAAAGTT CTTTAAAGCG GCCTCTAATT GTGGCATTGT AGAAAGCATA 
TTGAACTGGG TGAAATTTAA GGCTCAGACT CAGCTGAATA AGAAGTGCTC CTCAGTAAAG 
TACAGCAAAA TCAAAGGCAT TCCCAAACTG GATGATGCCA ATGATGCTGG TGGCAAACAT 
TCCCTGGAGT GTACATTAAT ATTAACAGAG GGGGACTCTG CCAAATCTCT AGCTGTCTCT 
GGATTAGGTG TGATTGGGAG AGACAGATAT GGAGTCTTCC CTCTCAGGGG TAAAATTCTC 
AATGCACGAG AAGCGTCTCA TAAACAGATT ATGGAAAATG CAGAAATAAA CAATATTATT 
AAAATAGTTG GTTTGCAATA CAAGAAAAGT TATGATGATG CAGAATCTCT GAAAACTTTA 
CGTTATGGGA AGATTATGAT TATGACTGAT CAGGATCAAG ATGGTTCTCA CATTAAAGGC 
CTGCTAATCA ATTTTATTCA TCACAATTGG CCATCACTTT TGAAGCATGG CTTTCTTGAA 
GAATTCATTA CTCCCATTGT AAAGGCGAGC AAAAATAAGC AGGAACTTTC CTTTTATAGT 
ATTCCTGAAT TTGATGAGTG GGAAAAACAT ATAGAAAACC AGAAAGCCTG GAAAATAAAG 
TACTACAAAG GATTGGGTAC CAGTACAGCT AAGGAAGCCA AGGAATATTT TGCTGATATG 
GAAAGGCATC GCATTTTGTT TAGATATGCT GGCCCTGAGG ATGATGCTGC CATTACCTTG 
GCATTTAGTA AGAAGAAGAT TGATGACAGA AAAGAATGGT TAACCAACTT TATGGAGGAT 
CGGAGACAGC GGAGGTTACA TGGCCTACCA GAGCAATTTT TATATGGTAC AGCAACTAAG 
CATTTGACAT ACAATGATTT CATCAACAAG GAATTGATTC TCTTCTCAAA CTCGGACAAT 
GAGAGATCTA TACCCTCTCT TGTGGATGGC TTCAAGCCAG GTCAACGAAA AGTTTTGTTT 
ACCTGTTTCA AGAGGAATGA TAAACGTGAA GTTAAAGTCG CACAGCTGGC TGGATCTGTT 
GCTGAAATGT CTGCTTATCA TCATGGAGAA CAAGCGTTGA TGATGACTAT TGTGAATTTG 
GCTCAGAATT TTGTGGGCAG CAACAACATT AACTTGCTTC AGCCCATTGG TCAGTTTGGA 
ACTCGGCTTC ACGGTGGCAA AGATGCTGCA AGCCCCCGTT ATATCTTCAC AATGTTAAGC 
TCTCTGGCAA GACTACTTTT TCCAGCTGTG GATGATAACT TGCTTAAGTT CCTTTATGAT 
GATAATCAGC GTGTAGAGCC GGAGTGGTAT ATCCCCATAA TCCCCATGGT TTTGATTAAT 
GGTGCTGAGG GTATTGGGAC TGGATGGGCT TGTAAATTGC CCAACTATGA TGCCAGAGAA 
ATTGTGAACA ATGTCAGGCG GATGCTAGAA GGTTTGGATC CTCACCCCAT GCTTCCAAAC 
TATAAGAACT TCAAAGGCAC CATCCAGGAG CTTGGTCAGA ACCAGTATGC TGTTAGTGGT 
GAGATATTTG TGGTGGACAG AAACACAGTA GAGATTACAG AACTTCCAGT TAGAACCTGG 
ACACAGGTAT ACAAGGAGCA GGTCCTAGAA CCTATGCTTA ATGGAACAGA TAAAACACCA 
GCACTGATTT CTGATTACAA AGAATATCAC ACTGATACAA CTGTGAAATT TGTGGTGAAG 
ATGACAGAAG AGAAGCTCGC CCAAGCGGAA GCTGCCGGCC TGCATAAAGT TTTTAAACTC 
CAAACCACTC TTACTTGCAA TTCCATGGTA CTCTTTGATC ATATGGGATG TCTGAAGAAG 
TATGAAACTG TGCAAGACAT TTTGAAAGAA TTCTTTGATT TACGGTTAAG TTATTATGGT 
TTACGGAAGG AGTGGCTTGT AGGAATGTTG GGAGCAGAAT CCACAAAGCT TAACAATCAA 
GCCCGTTTCA TTTTAGAGAA GATACAAGGG AAAATCACGA TAGAGAATAG GTCAAAGAAA 
GATTTGATTC AAATGTTAGT CCAGAGAGGT TATGAATCTG ACCCAGTGAA AGCCTGGAAA 
GAAGCACAAG AAAAGGCTGC GGAAGAGGAA GATGCACAAA ACCCACATGA TGACAGTTCC 
TCTGACTCAG GAACTCCTTC AGGCCCTGAT TTTAATTATA TTTTAAATAT GTCTCTATGG 
TCTCTTACTA AAGAAAAAGT TGAAGAACTC ATTAAACAGA GAGATACAAA AGGACGAGAG 
GTTAATGATC TCAAAAGGAA ATCTCCCTCA GATCTTTGGA AAGAAGATTT AGCAGCATTT 
GTTGAAGAAT TGGATAAAGT GGAAGCACAA GAACGAGAAG ACATTCTGGC AGGGATGTCT 
GGGAAAGCAG TGAAAGGCAA AGTCGGCAAA GCTAAAGTAA AGAAGCTCCA GCTGGAGGAG 
ACGATGCCCT CGCCTTACGG GAGAAGAATC GTTCCTGAGA TTACTGCCAT GAAGGCAGAC 
GCCAGCAGAA AACTGCTGAA GAAGAAGAAG GGTGATCCTG ATACTACAGT TGTGAAAGTA 
GAATTTGATG AAGAATTTAG TGGAACACCA GCAGAAGGTA CAGGAGAAGA GACATTGACA 
CCATCTGCTC CTGTAAATAA AGGCCCCAAA CCCAAAAGGG AGAAAAAGGA GCCTGGTACC 
AGGGTTAGAA AAACACCTGC ATCAACTGGC AAACCTAATA CAAAGAAAGT GAAGAAGCGG 
AATCCTTGGT CAGATGATGA GTCCAAATCA GAGAGTGACT TGGAAGAAGC AGAGCCTGTG 
GTTATCCCAA GAGATTCTTT GCTTAGGAGA GCAGCTGCTG AAAGACCTAA GTACACTTTT 
GATTTCTCAG AAGAGGAGGA GGATGATGCT GATGATGATG ACGACAATAA TGATTTAGAA 
GAATTGAAAG TTAAAGCATC TCCCATAACA AATGATGGGG AGGATGAATT TGTTCCTTCA 
GATGGCATAG ATAAAGATGA GTATGCCTTT TCTCCAGGCA AATCCAAAGC TACACCAGAA 
AAATCTTCAC ATGACAAAAA AAGTCAGGAT TTTGGAAATC TTTTTTCATT TCCTTCATAT 
TCTCAGAAGT CAGAAGATGA TTCAGCTAAA TTTGACAGTA ATGAAGAAGA CACCACATCT 
GTTTTTGCAC CATCATTTGG TCTGAAGCAA ACAGATAAAG TTCCAAGTCA AACAGTCGCT 
GCTAAAAAGG GTAAAGCACC TTCAGATGCA GCAGCCCCTA AGGCCAAGAG AGCCCCTAGA 
CAGAGGAAGG TAGTAGAGCC TGCAAACTCC GACTCGGATT CTGAACTCGG CAACATTCCA 
AAGAAGACGG CCGCACCGAA AGGTAAAGGC CGAGGGGCAA AGAAAAGGAA AGCATCAGGC 
TCAGAAAATG AAGGTGATTA TAACCCTGGC AGGAAACCAT CTAAAACAGC CAGCAAGAAA 
CCGAAGAAAA CGTCTTTTGA TCAGGATTCA GATGTAGACA TCTTCCCTTC AGACTTCACT 
TCTGAACCAC CTGCTCTCCC ACGGACTGGT CGGGCTAGGA AAGAAGTAAA ATATTTTGCA 
GAGTCTGATG AAGAAGAAGA CGTTGATTTT GCAATGTTTA ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001094328.1
CDS	1..4845
Translation	MAKSSLAGVDGALTWVNNAAKKEELETANKNDSSKKLSVERVYQKKTQLEHILLRPDTYIGSVEPLTQLMWVYDEDVGMNCREVTFVPGLYKIFDEILVNAADNKQRDKNMTCIKVSIDPESNIISIWNNGKGIPVVEHKVEKVYVPALIFGQLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTKFTVETACKEYKHSFKQTWMNNMMKTSEAKIKHFDGEDYTCITFQPDLAKFKMEKLDKDIVALMTRRAYDLAGSCKGVKVMFNGKKLPVNGFRSYVDLYVKDKLDETGVALKVIHELANERWDVCLTLSEKGFQQISFVNSIATTKGGRHVDYVVDQVVGKLIEVVKKKNKAGVSVKPFQVKNHIWVFINCLIENPTFDSQTKENMTLQPKSFGSKCQLSEKFFKAASNCGIVESILNWVKFKAQTQLNKKCSSVKYSKIKGIPKLDDANDAGGKHSLECTLILTEGDSAKSLAVSGLGVIGRDRYGVFPLRGKILNAREASHKQIMENAEINNIIKIVGLQYKKSYDDAESLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLKHGFLEEFITPIVKASKNKQELSFYSIPEFDEWEKHIENQKAWKIKYYKGLGTSTAKEAKEYFADMERHRILFRYAGPEDDAAITLAFSKKKIDDRKEWLTNFMEDRRQRRLHGLPEQFLYGTATKHLTYNDFINKELILFSNSDNERSIPSLVDGFKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSAYHHGEQALMMTIVNLAQNFVGSNNINLLQPIGQFGTRLHGGKDAASPRYIFTMLSSLARLLFPAVDDNLLKFLYDDNQRVEPEWYIPIIPMVLINGAEGIGTGWACKLPNYDAREIVNNVRRMLEGLDPHPMLPNYKNFKGTIQELGQNQYAVSGEIFVVDRNTVEITELPVRTWTQVYKEQVLEPMLNGTDKTPALISDYKEYHTDTTVKFVVKMTEEKLAQAEAAGLHKVFKLQTTLTCNSMVLFDHMGCLKKYETVQDILKEFFDLRLSYYGLRKEWLVGMLGAESTKLNNQARFILEKIQGKITIENRSKKDLIQMLVQRGYESDPVKAWKEAQEKAAEEEDAQNPHDDSSSDSGTPSGPDFNYILNMSLWSLTKEKVEELIKQRDTKGREVNDLKRKSPSDLWKEDLAAFVEELDKVEAQEREDILAGMSGKAVKGKVGKAKVKKLQLEETMPSPYGRRIVPEITAMKADASRKLLKKKKGDPDTTVVKVEFDEEFSGTPAEGTGEETLTPSAPVNKGPKPKREKKEPGTRVRKTPASTGKPNTKKVKKRNPWSDDESKSESDLEEAEPVVIPRDSLLRRAAAERPKYTFDFSEEEEDDADDDDDNNDLEELKVKASPITNDGEDEFVPSDGIDKDEYAFSPGKSKATPEKSSHDKKSQDFGNLFSFPSYSQKSEDDSAKFDSNEEDTTSVFAPSFGLKQTDKVPSQTVAAKKGKAPSDAAAPKAKRAPRQRKVVEPANSDSDSELGNIPKKTAAPKGKGRGAKKRKASGSENEGDYNPGRKPSKTASKKPKKTSFDQDSDVDIFPSDFTSEPPALPRTGRARKEVKYFAESDEEEDVDFAMFN

Target ORF information:

RefSeq Version	NM_001100858.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus DNA topoisomerase II beta (Top2b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001100858.1

ORF Insert Sequence:

ATGGCCAAGT CCAGCCTCGC GGGCGTTGAC GGGGCGCTGA CCTGGGTGAA CAATGCTGCA 
AAAAAGGAAG AGTTGGAGAC AGCGAACAAA AATGATTCTT CCAAGAAGTT GTCTGTTGAG 
AGAGTGTACC AGAAGAAGAC CCAACTGGAG CATATTCTCC TTCGTCCTGA TACATACATT 
GGATCAGTGG AACCACTGAC ACAGTTAATG TGGGTGTATG ACGAAGATGT AGGCATGAAC 
TGCAGAGAGG TTACCTTTGT TCCGGGCTTA TACAAGATCT TTGATGAAAT TTTGGTCAAT 
GCTGCTGACA ACAAGCAGAG GGATAAGAAC ATGACCTGTA TTAAGGTTTC TATTGATCCT 
GAATCTAATA TTATAAGCAT TTGGAATAAT GGAAAAGGCA TTCCAGTAGT AGAACACAAA 
GTAGAGAAAG TTTATGTTCC TGCTTTAATT TTTGGACAGC TTTTAACATC GAGTAATTAC 
GATGATGATG AGAAAAAAGT TACAGGAGGC CGTAATGGTT ATGGTGCAAA ACTTTGTAAT 
ATTTTTAGTA CAAAGTTTAC AGTAGAAACG GCTTGCAAAG AATACAAACA TAGTTTTAAG 
CAGACATGGA TGAATAATAT GATGAAGACT TCTGAAGCCA AAATCAAACA TTTTGATGGG 
GAAGATTATA CATGCATAAC ATTCCAGCCA GATCTAGCCA AGTTTAAGAT GGAAAAACTT 
GACAAGGACA TTGTGGCCCT CATGACCAGA AGGGCATACG ATTTAGCTGG TTCCTGCAAA 
GGCGTCAAGG TCATGTTCAA TGGCAAGAAG TTGCCTGTAA ATGGATTTCG CAGCTATGTA 
GACCTTTATG TGAAAGACAA ATTGGATGAA ACTGGGGTCG CTCTCAAAGT TATTCATGAA 
CTTGCAAATG AAAGATGGGA TGTTTGTCTA ACCTTGAGTG AAAAAGGTTT CCAGCAGATC 
AGCTTTGTGA ATAGTATTGC AACTACAAAA GGTGGACGGC ACGTGGATTA CGTAGTAGAT 
CAAGTTGTTG GTAAACTGAT TGAAGTAGTT AAGAAAAAGA ACAAAGCTGG TGTATCAGTG 
AAGCCATTTC AAGTTAAAAA CCACATATGG GTTTTTATTA ATTGTCTTAT TGAAAATCCA 
ACCTTTGATT CTCAAACTAA GGAAAACATG ACTCTGCAGC CCAAGAGCTT TGGGTCTAAA 
TGCCAGCTGT CAGAAAAGTT CTTTAAAGCG GCCTCTAATT GTGGCATTGT AGAAAGCATA 
TTGAACTGGG TGAAATTTAA GGCTCAGACT CAGCTGAATA AGAAGTGCTC CTCAGTAAAG 
TACAGCAAAA TCAAAGGCAT TCCCAAACTG GATGATGCCA ATGATGCTGG TGGCAAACAT 
TCCCTGGAGT GTACATTAAT ATTAACAGAG GGGGACTCTG CCAAATCTCT AGCTGTCTCT 
GGATTAGGTG TGATTGGGAG AGACAGATAT GGAGTCTTCC CTCTCAGGGG TAAAATTCTC 
AATGCACGAG AAGCGTCTCA TAAACAGATT ATGGAAAATG CAGAAATAAA CAATATTATT 
AAAATAGTTG GTTTGCAATA CAAGAAAAGT TATGATGATG CAGAATCTCT GAAAACTTTA 
CGTTATGGGA AGATTATGAT TATGACTGAT CAGGATCAAG ATGGTTCTCA CATTAAAGGC 
CTGCTAATCA ATTTTATTCA TCACAATTGG CCATCACTTT TGAAGCATGG CTTTCTTGAA 
GAATTCATTA CTCCCATTGT AAAGGCGAGC AAAAATAAGC AGGAACTTTC CTTTTATAGT 
ATTCCTGAAT TTGATGAGTG GGAAAAACAT ATAGAAAACC AGAAAGCCTG GAAAATAAAG 
TACTACAAAG GATTGGGTAC CAGTACAGCT AAGGAAGCCA AGGAATATTT TGCTGATATG 
GAAAGGCATC GCATTTTGTT TAGATATGCT GGCCCTGAGG ATGATGCTGC CATTACCTTG 
GCATTTAGTA AGAAGAAGAT TGATGACAGA AAAGAATGGT TAACCAACTT TATGGAGGAT 
CGGAGACAGC GGAGGTTACA TGGCCTACCA GAGCAATTTT TATATGGTAC AGCAACTAAG 
CATTTGACAT ACAATGATTT CATCAACAAG GAATTGATTC TCTTCTCAAA CTCGGACAAT 
GAGAGATCTA TACCCTCTCT TGTGGATGGC TTCAAGCCAG GTCAACGAAA AGTTTTGTTT 
ACCTGTTTCA AGAGGAATGA TAAACGTGAA GTTAAAGTCG CACAGCTGGC TGGATCTGTT 
GCTGAAATGT CTGCTTATCA TCATGGAGAA CAAGCGTTGA TGATGACTAT TGTGAATTTG 
GCTCAGAATT TTGTGGGCAG CAACAACATT AACTTGCTTC AGCCCATTGG TCAGTTTGGA 
ACTCGGCTTC ACGGTGGCAA AGATGCTGCA AGCCCCCGTT ATATCTTCAC AATGTTAAGC 
TCTCTGGCAA GACTACTTTT TCCAGCTGTG GATGATAACT TGCTTAAGTT CCTTTATGAT 
GATAATCAGC GTGTAGAGCC GGAGTGGTAT ATCCCCATAA TCCCCATGGT TTTGATTAAT 
GGTGCTGAGG GTATTGGGAC TGGATGGGCT TGTAAATTGC CCAACTATGA TGCCAGAGAA 
ATTGTGAACA ATGTCAGGCG GATGCTAGAA GGTTTGGATC CTCACCCCAT GCTTCCAAAC 
TATAAGAACT TCAAAGGCAC CATCCAGGAG CTTGGTCAGA ACCAGTATGC TGTTAGTGGT 
GAGATATTTG TGGTGGACAG AAACACAGTA GAGATTACAG AACTTCCAGT TAGAACCTGG 
ACACAGGTAT ACAAGGAGCA GGTCCTAGAA CCTATGCTTA ATGGAACAGA TAAAACACCA 
GCACTGATTT CTGATTACAA AGAATATCAC ACTGATACAA CTGTGAAATT TGTGGTGAAG 
ATGACAGAAG AGAAGCTCGC CCAAGCGGAA GCTGCCGGCC TGCATAAAGT TTTTAAACTC 
CAAACCACTC TTACTTGCAA TTCCATGGTA CTCTTTGATC ATATGGGATG TCTGAAGAAG 
TATGAAACTG TGCAAGACAT TTTGAAAGAA TTCTTTGATT TACGGTTAAG TTATTATGGT 
TTACGGAAGG AGTGGCTTGT AGGAATGTTG GGAGCAGAAT CCACAAAGCT TAACAATCAA 
GCCCGTTTCA TTTTAGAGAA GATACAAGGG AAAATCACGA TAGAGAATAG GTCAAAGAAA 
GATTTGATTC AAATGTTAGT CCAGAGAGGT TATGAATCTG ACCCAGTGAA AGCCTGGAAA 
GAAGCACAAG AAAAGGCTGC GGAAGAGGAA GATGCACAAA ACCCACATGA TGACAGTTCC 
TCTGACTCAG GAACTCCTTC AGGCCCTGAT TTTAATTATA TTTTAAATAT GTCTCTATGG 
TCTCTTACTA AAGAAAAAGT TGAAGAACTC ATTAAACAGA GAGATACAAA AGGACGAGAG 
GTTAATGATC TCAAAAGGAA ATCTCCCTCA GATCTTTGGA AAGAAGATTT AGCAGCATTT 
GTTGAAGAAT TGGATAAAGT GGAAGCACAA GAACGAGAAG ACATTCTGGC AGGGATGTCT 
GGGAAAGCAG TGAAAGGCAA AGTCGGCAAA GCTAAAGTAA AGAAGCTCCA GCTGGAGGAG 
ACGATGCCCT CGCCTTACGG GAGAAGAATC GTTCCTGAGA TTACTGCCAT GAAGGCAGAC 
GCCAGCAGAA AACTGCTGAA GAAGAAGAAG GGTGATCCTG ATACTACAGT TGTGAAAGTA 
GAATTTGATG AAGAATTTAG TGGAACACCA GCAGAAGGTA CAGGAGAAGA GACATTGACA 
CCATCTGCTC CTGTAAATAA AGGCCCCAAA CCCAAAAGGG AGAAAAAGGA GCCTGGTACC 
AGGGTTAGAA AAACACCTGC ATCAACTGGC AAACCTAATA CAAAGAAAGT GAAGAAGCGG 
AATCCTTGGT CAGATGATGA GTCCAAATCA GAGAGTGACT TGGAAGAAGC AGAGCCTGTG 
GTTATCCCAA GAGATTCTTT GCTTAGGAGA GCAGCTGCTG AAAGACCTAA GTACACTTTT 
GATTTCTCAG AAGAGGAGGA GGATGATGCT GATGATGATG ACGACAATAA TGATTTAGAA 
GAATTGAAAG TTAAAGCATC TCCCATAACA AATGATGGGG AGGATGAATT TGTTCCTTCA 
GATGGCATAG ATAAAGATGA GTATGCCTTT TCTCCAGGCA AATCCAAAGC TACACCAGAA 
AAATCTTCAC ATGACAAAAA AAGTCAGGAT TTTGGAAATC TTTTTTCATT TCCTTCATAT 
TCTCAGAAGT CAGAAGATGA TTCAGCTAAA TTTGACAGTA ATGAAGAAGA CACCACATCT 
GTTTTTGCAC CATCATTTGG TCTGAAGCAA ACAGATAAAG TTCCAAGTCA AACAGTCGCT 
GCTAAAAAGG GTAAAGCACC TTCAGATGCA GCAGCCCCTA AGGCCAAGAG AGCCCCTAGA 
CAGAGGAAGG TAGTAGAGCC TGCAAACTCC GACTCGGATT CTGAACTCGG CAACATTCCA 
AAGAAGACGG CCGCACCGAA AGGTAAAGGC CGAGGGGCAA AGAAAAGGAA AGCATCAGGC 
TCAGAAAATG AAGGTGATTA TAACCCTGGC AGGAAACCAT CTAAAACAGC CAGCAAGAAA 
CCGAAGAAAA CGTCTTTTGA TCAGGATTCA GATGTAGACA TCTTCCCTTC AGACTTCACT 
TCTGAACCAC CTGCTCTCCC ACGGACTGGT CGGGCTAGGA AAGAAGTAAA ATATTTTGCA 
GAGTCTGATG AAGAAGAAGA CGTTGATTTT GCAATGTTTA ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Anthracyclines/trastuzumab: new aspects of cardiotoxicity and molecular mechanisms.
Trends in pharmacological sciences36(6)326-48(2015 Jun)
Rochette L,Guenancia C,Gudjoncik A,Hachet O,Zeller M,Cottin Y,Vergely C

Nuclear dynamics of topoisomerase II? reflects its catalytic activity that is regulated by binding of RNA to the C-terminal domain.
Nucleic acids research42(14)9005-20(2014 Aug)
Onoda A,Hosoya O,Sano K,Kiyama K,Kimura H,Kawano S,Furuta R,Miyaji M,Tsutsui K,Tsutsui KM

Doxorubicin, DNA torsion, and chromatin dynamics.
Biochimica et biophysica acta1845(1)84-9(2014 Jan)
Yang F,Teves SS,Kemp CJ,Henikoff S

Catalytic inhibitors of topoisomerase II differently modulate the toxicity of anthracyclines in cardiac and cancer cells.
PloS one8(10)e76676(2013)
Vavrova A,Jansova H,Mackova E,Machacek M,Haskova P,Tichotova L,Sterba M,Simunek T