Ube2e2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ube2e2
Entrez Gene ID	361013
Full Name	ubiquitin-conjugating enzyme E2E 2
General protein information	Preferred Names ubiquitin-conjugating enzyme E2E 2 Names ubiquitin-conjugating enzyme E2 E2 ubiquitin-conjugating enzyme E2E 2 (UBC4/5 homolog, yeast)
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 15 Map Location: 15p16

mRNA and Protein(s)

mRNA	Protein	Name
XM_006251699.3	XP_006251761.3	ubiquitin-conjugating enzyme E2 E2 isoform X1
NM_001108371.1	NP_001101841.1	ubiquitin-conjugating enzyme E2 E2

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Ube2e2	NP_659088.1
Rattus norvegicus (Norway rat)	Ube2e2	NP_001101841.1
Drosophila melanogaster (fruit fly)	UbcD2	NP_001260362.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007952	XP_317521.4
Homo sapiens (human)	UBE2E2	NP_689866.1
	UBE2E2	XP_001089978.1
Bos taurus (cattle)	UBE2E2	NP_001178119.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012754	XP_306962.4
Xenopus tropicalis (tropical clawed frog)	ube2e2	NP_001119983.1
Pan troglodytes (chimpanzee)	UBE2E2	XP_003309701.1
Canis lupus familiaris (dog)	UBE2E2	XP_005634421.1
Gallus gallus (chicken)	UBE2E2	XP_004939459.1
Danio rerio (zebrafish)	ube2e2	NP_001003494.1

Gene Ontology
Bibliography

Related articles in PubMed

Identification of epigenetically downregulated Tmem70 and Ube2e2 in rat liver after 28-day treatment with hepatocarcinogenic thioacetamide showing gene product downregulation in hepatocellular preneoplastic and neoplastic lesions produced by tumor promotion.
Mizukami S, Yafune A, Watanabe Y, Nakajima K, Jin M, Yoshida T, Shibutani M
Toxicology letters26613-22(2017 Jan)

Selective ubiquitylation of p21 and Cdt1 by UBCH8 and UBE2G ubiquitin-conjugating enzymes via the CRL4Cdt2 ubiquitin ligase complex.
Shibata E, Abbas T, Huang X, Wohlschlegel JA, Dutta A
Molecular and cellular biology31(15)3136-45(2011 Aug)

The E2 ubiquitin-conjugating enzymes direct polyubiquitination to preferred lysines.
David Y, Ziv T, Admon A, Navon A
The Journal of biological chemistry285(12)8595-604(2010 Mar)

Link between the ubiquitin conjugation system and the ISG15 conjugation system: ISG15 conjugation to the UbcH6 ubiquitin E2 enzyme.
Takeuchi T, Iwahara S, Saeki Y, Sasajima H, Yokosawa H
Journal of biochemistry138(6)711-9(2005 Dec)

cDNA cloning, characterization, and chromosome mapping of UBE2E2 encoding a human ubiquitin-conjugating E2 enzyme.
Kimura M, Hattori T, Matsuda Y, Yoshioka T, Sumi N, Umeda Y, Nakashima S, Okano Y
Cytogenetics and cell genetics78(2)107-11(1997)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The effect of thioacetamide on neoplastic liver changes and hypermethylation and downregulation of proteins, especially Tmem70 and Ube2e2, are reported.
Title: Identification of epigenetically downregulated Tmem70 and Ube2e2 in rat liver after 28-day treatment with hepatocarcinogenic thioacetamide showing gene product downregulation in hepatocellular preneoplastic and neoplastic lesions produced by tumor promotion.

The following Ube2e2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ube2e2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa11708	NM_001108371.1 Latest version!	Rattus norvegicus ubiquitin-conjugating enzyme E2E 2 (Ube2e2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
ORa58611	XM_006251699.3 Latest version!	Rattus norvegicus ubiquitin-conjugating enzyme E2E 2 (Ube2e2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ube2e2 ( NM_001108371.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001101841.1 Rattus norvegicus ubiquitin-conjugating enzyme E2E 2 (Ube2e2), mRNA.

Price & Availability↑

CloneID	ORa11708
Clone ID Related Accession (Same CDS sequence)	NM_001108371.1
Accession Version	NM_001108371.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 555bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-27
Organism	Rattus norvegicus(Norway rat)
Product	ubiquitin-conjugating enzyme E2 E2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH474010.1. On or before Oct 4, 2007 this sequence version replaced XM_341288.3, XM_001067024.1. ##Evidence-Data-START## Transcript exon combination :: CB608770.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMEA2689598 [ECO:0000348] ##Evidence-Data-END##

ATGTCCACCG AGGCACAGAG AGTTGATGAC AGTCCAAGCA CTAGCGGAGG AAGTTCTGAT 
GGCGATCAAC GGGAGAGTGT TCAGCAGGAA CCTGAGCGGG AGCAAGTTCA GCCCAAGAAG 
AAGGAGGGGA AGATATCCAG CAAGACGGCT GCGAAGCTGT CGACCAGTGC CAAAAGTGCT 
GGACCCAAAG GAGACAACAT TTATGAATGG AGGTCAACTA TATTGGGACC CCCAGGATCT 
GTCTATGAAG GAGGGGTGTT CTTTCTTGAC ATTACCTTTT CACCAGACTA TCCGTTTAAA 
CCCCCTAAGG TCACCTTCCG AACAAGAATC TATCACTGTA ATATCAACAG CCAGGGCGTG 
ATCTGCCTGG ACATCCTCAA GGACAACTGG AGTCCGGCCC TGACCATCTC TAAAGTCCTC 
CTGTCCATCT GCTCACTACT TACAGACTGC AACCCTGCCG ACCCTCTGGT AGGCAGTATT 
GCCACACAGT ACATGACCAA CAGAGCCGAG CACGACCGGA TGGCCAGACA GTGGACCAAG 
CGGTACGCCA CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001101841.1
CDS	14..568
Translation	MSTEAQRVDDSPSTSGGSSDGDQRESVQQEPEREQVQPKKKEGKISSKTAAKLSTSAKSAGPKGDNIYEWRSTILGPPGSVYEGGVFFLDITFSPDYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVLLSICSLLTDCNPADPLVGSIATQYMTNRAEHDRMARQWTKRYAT

Target ORF information:

RefSeq Version	NM_001108371.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ubiquitin-conjugating enzyme E2E 2 (Ube2e2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001108371.1

ORF Insert Sequence:

ATGTCCACCG AGGCACAGAG AGTTGATGAC AGTCCAAGCA CTAGCGGAGG AAGTTCTGAT 
GGCGATCAAC GGGAGAGTGT TCAGCAGGAA CCTGAGCGGG AGCAAGTTCA GCCCAAGAAG 
AAGGAGGGGA AGATATCCAG CAAGACGGCT GCGAAGCTGT CGACCAGTGC CAAAAGTGCT 
GGACCCAAAG GAGACAACAT TTATGAATGG AGGTCAACTA TATTGGGACC CCCAGGATCT 
GTCTATGAAG GAGGGGTGTT CTTTCTTGAC ATTACCTTTT CACCAGACTA TCCGTTTAAA 
CCCCCTAAGG TCACCTTCCG AACAAGAATC TATCACTGTA ATATCAACAG CCAGGGCGTG 
ATCTGCCTGG ACATCCTCAA GGACAACTGG AGTCCGGCCC TGACCATCTC TAAAGTCCTC 
CTGTCCATCT GCTCACTACT TACAGACTGC AACCCTGCCG ACCCTCTGGT AGGCAGTATT 
GCCACACAGT ACATGACCAA CAGAGCCGAG CACGACCGGA TGGCCAGACA GTGGACCAAG 
CGGTACGCCA CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ube2e2 ( XM_006251699.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006251761.3 Rattus norvegicus ubiquitin-conjugating enzyme E2E 2 (Ube2e2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa58611
Clone ID Related Accession (Same CDS sequence)	XM_006251699.3
Accession Version	XM_006251699.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 633bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	ubiquitin-conjugating enzyme E2 E2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005114.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006251699.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATTCT TTAACCCAGG ATCTAAGATG TCCACCGAGG CACAGAGAGT TGATGACAGT 
CCAAGCACTA GCGGAGGAAG TTCTGATGGC GATCAACGGG AGAGTGTTCA GCAGGAACCT 
GAGCGGGAGC AAGTTCAGCC CAAGAAGAAG GAGGGGAAGA TATCCAGCAA GACGGCTGCG 
AAGCTGTCGA CCAGTGCCAA AAGAATTCAG AAGGAGCTTG CAGAAATCAC GCTGGACCCC 
CCTCCCAACT GTAGTGCTGG ACCCAAAGGA GACAACATTT ATGAATGGAG GTCAACTATA 
TTGGGACCCC CAGGATCTGT CTATGAAGGA GGGGTGTTCT TTCTTGACAT TACCTTTTCA 
CCAGACTATC CGTTTAAACC CCCTAAGGTC ACCTTCCGAA CAAGAATCTA TCACTGTAAT 
ATCAACAGCC AGGGCGTGAT CTGCCTGGAC ATCCTCAAGG ACAACTGGAG TCCGGCCCTG 
ACCATCTCTA AAGTCCTCCT GTCCATCTGC TCACTACTTA CAGACTGCAA CCCTGCCGAC 
CCTCTGGTAG GCAGTATTGC CACACAGTAC ATGACCAACA GAGCCGAGCA CGACCGGATG 
GCCAGACAGT GGACCAAGCG GTACGCCACC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006251761.3
CDS	24..656
Translation	MSFFNPGSKMSTEAQRVDDSPSTSGGSSDGDQRESVQQEPEREQVQPKKKEGKISSKTAAKLSTSAKRIQKELAEITLDPPPNCSAGPKGDNIYEWRSTILGPPGSVYEGGVFFLDITFSPDYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVLLSICSLLTDCNPADPLVGSIATQYMTNRAEHDRMARQWTKRYAT

Target ORF information:

RefSeq Version	XM_006251699.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ubiquitin-conjugating enzyme E2E 2 (Ube2e2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006251699.3

ORF Insert Sequence:

ATGTCATTCT TTAACCCAGG ATCTAAGATG TCCACCGAGG CACAGAGAGT TGATGACAGT 
CCAAGCACTA GCGGAGGAAG TTCTGATGGC GATCAACGGG AGAGTGTTCA GCAGGAACCT 
GAGCGGGAGC AAGTTCAGCC CAAGAAGAAG GAGGGGAAGA TATCCAGCAA GACGGCTGCG 
AAGCTGTCGA CCAGTGCCAA AAGAATTCAG AAGGAGCTTG CAGAAATCAC GCTGGACCCC 
CCTCCCAACT GTAGTGCTGG ACCCAAAGGA GACAACATTT ATGAATGGAG GTCAACTATA 
TTGGGACCCC CAGGATCTGT CTATGAAGGA GGGGTGTTCT TTCTTGACAT TACCTTTTCA 
CCAGACTATC CGTTTAAACC CCCTAAGGTC ACCTTCCGAA CAAGAATCTA TCACTGTAAT 
ATCAACAGCC AGGGCGTGAT CTGCCTGGAC ATCCTCAAGG ACAACTGGAG TCCGGCCCTG 
ACCATCTCTA AAGTCCTCCT GTCCATCTGC TCACTACTTA CAGACTGCAA CCCTGCCGAC 
CCTCTGGTAG GCAGTATTGC CACACAGTAC ATGACCAACA GAGCCGAGCA CGACCGGATG 
GCCAGACAGT GGACCAAGCG GTACGCCACC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Identification of epigenetically downregulated Tmem70 and Ube2e2 in rat liver after 28-day treatment with hepatocarcinogenic thioacetamide showing gene product downregulation in hepatocellular preneoplastic and neoplastic lesions produced by tumor promotion.
Toxicology letters26613-22(2017 Jan)
Mizukami S,Yafune A,Watanabe Y,Nakajima K,Jin M,Yoshida T,Shibutani M

Selective ubiquitylation of p21 and Cdt1 by UBCH8 and UBE2G ubiquitin-conjugating enzymes via the CRL4Cdt2 ubiquitin ligase complex.
Molecular and cellular biology31(15)3136-45(2011 Aug)
Shibata E,Abbas T,Huang X,Wohlschlegel JA,Dutta A

The E2 ubiquitin-conjugating enzymes direct polyubiquitination to preferred lysines.
The Journal of biological chemistry285(12)8595-604(2010 Mar)
David Y,Ziv T,Admon A,Navon A

Link between the ubiquitin conjugation system and the ISG15 conjugation system: ISG15 conjugation to the UbcH6 ubiquitin E2 enzyme.
Journal of biochemistry138(6)711-9(2005 Dec)
Takeuchi T,Iwahara S,Saeki Y,Sasajima H,Yokosawa H

cDNA cloning, characterization, and chromosome mapping of UBE2E2 encoding a human ubiquitin-conjugating E2 enzyme.
Cytogenetics and cell genetics78(2)107-11(1997)
Kimura M,Hattori T,Matsuda Y,Yoshioka T,Sumi N,Umeda Y,Nakashima S,Okano Y