Spg7 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Spg7
Entrez Gene ID	353231
Full Name	SPG7, paraplegin matrix AAA peptidase subunit
General protein information	Preferred Names SPG7, paraplegin matrix AAA peptidase subunit Names paraplegin spastic paraplegia 7 homolog
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 19 Map Location: 19q12

mRNA and Protein(s)

mRNA	Protein	Name
XM_017601307.1	XP_017456796.1	paraplegin isoform X2
XM_008772621.2	XP_008770843.1	paraplegin isoform X3
XM_008772619.2	XP_008770841.1	paraplegin isoform X1
NM_181388.2	NP_852053.1	paraplegin

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	SPG7	XP_001140249.3
	SPG7	XP_002802648.1
Rattus norvegicus (Norway rat)	Spg7	NP_852053.1
Danio rerio (zebrafish)	spg7	XP_001923118.1
Gallus gallus (chicken)	SPG7	NP_001012545.1
Drosophila melanogaster (fruit fly)	CG2658	NP_570017.1
Caenorhabditis elegans (roundworm)	ppgn-1	NP_500191.3
Mus musculus (house mouse)	Spg7	NP_694816.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000560	XP_310523.5
Homo sapiens (human)	SPG7	NP_003110.1
Canis lupus familiaris (dog)	SPG7	XP_546777.3
Bos taurus (cattle)	SPG7	NP_001095492.1
Xenopus tropicalis (tropical clawed frog)	spg7	XP_002933705.1

Gene Ontology
Bibliography

Related articles in PubMed

SPG7 Is an Essential and Conserved Component of the Mitochondrial Permeability Transition Pore.
Shanmughapriya S, Rajan S, Hoffman NE, Higgins AM, Tomar D, Nemani N, Hines KJ, Smith DJ, Eguchi A, Vallem S, Shaikh F, Cheung M, Leonard NJ, Stolakis RS, Wolfers MP, Ibetti J, Chuprun JK, Jog NR, Houser SR, Koch WJ, Elrod JW, Madesh M
Molecular cell60(1)47-62(2015 Oct)

Autocatalytic processing of m-AAA protease subunits in mitochondria.
Koppen M, Bonn F, Ehses S, Langer T
Molecular biology of the cell20(19)4216-24(2009 Oct)

Axonal degeneration in paraplegin-deficient mice is associated with abnormal mitochondria and impairment of axonal transport.
Ferreirinha F, Quattrini A, Pirozzi M, Valsecchi V, Dina G, Broccoli V, Auricchio A, Piemonte F, Tozzi G, Gaeta L, Casari G, Ballabio A, Rugarli EI
The Journal of clinical investigation113(2)231-42(2004 Jan)

Integrated analysis of protein composition, tissue diversity, and gene regulation in mouse mitochondria.
Mootha VK, Bunkenborg J, Olsen JV, Hjerrild M, Wisniewski JR, Stahl E, Bolouri MS, Ray HN, Sihag S, Kamal M, Patterson N, Lander ES, Mann M
Cell115(5)629-40(2003 Nov)

Molecular and functional analyses of the human and mouse genes encoding AFG3L1, a mitochondrial metalloprotease homologous to the human spastic paraplegia protein.
Kremmidiotis G, Gardner AE, Settasatian C, Savoia A, Sutherland GR, Callen DF
Genomics76(1-3)58-65(2001 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Spg7 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Spg7 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa47647	XM_008772621.2 Latest version!	Rattus norvegicus SPG7, paraplegin matrix AAA peptidase subunit (Spg7), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa12810	NM_181388.2 Latest version!	Rattus norvegicus SPG7 matrix AAA peptidase subunit, paraplegin (Spg7), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$440.30 $629.00
ORa47645	XM_008772619.2 Latest version!	Rattus norvegicus SPG7, paraplegin matrix AAA peptidase subunit (Spg7), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa59450	XM_017601307.1 Latest version!	Rattus norvegicus SPG7, paraplegin matrix AAA peptidase subunit (Spg7), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Spg7 ( XM_008772621.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008770843.1 Rattus norvegicus SPG7, paraplegin matrix AAA peptidase subunit (Spg7), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa47647
Clone ID Related Accession (Same CDS sequence)	XM_008772621.2
Accession Version	XM_008772621.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1938bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	paraplegin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005118.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008772621.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACCGGG AGAGGCTGCG CACCTTGTTC ATCATCGCCA TCGTGATGAG CCTGCTCAAC 
TCCCTCAGCA CAAGTGGCGG CAGCATCTCC TGGGCTGACT TCGTCAACGA GATGCTGGCT 
AAAGGCGAGG TGCAGCGTGT GCAGGTGGTG CCCGAGAGTG ATGTGGTGGA AGTCTACCTG 
CATCCAGGAG CTGTGGTGTT TGGGCGGCCT CGGCTGGCCT TGATGTACCG GATGCAGGTT 
GCAAACATCG ACAAATTTGA AGAGAAGCTT CGAGCTGCTG AAGATGAACT GAACATCGAG 
AGCAAGGACA GGATTCCCGT GTCCTACAAG CGGACAGGAT TCTTTGGAAA TGCCCTCTAT 
GCCCTGGGGA TGACTGCAGT GGGCTTGGCC ATCCTGTGGT ATGTTTTCAG ACTGGCTGGG 
ATGACCGGAA GGGAAGGCGG ATTCAGTGCC TTTAATCAGC TTAAAATGGC GCGCTTCACC 
ATCGTGGACG GGAAGACGGG GAAAGGAGTC AGCTTCCAGG ATGTGGCGGG AATGCACGAA 
GCCAAGCTGG AAGTCCGAGA GTTTGTGGAT TATCTGAAGA GCCCGGAGCG TTTCCTTCAG 
CTGGGTGCCA AGGTTCCAAA GGGTGCCCTG CTGCTGGGAC CCCCTGGTTG TGGGAAGACG 
CTGTTGGCCA AGGCAGTGGC CACGGAGGCT CAGGTGCCCT TTTTAGCAAT GGCTGGCCCA 
GAGTTTGTGG AGGTCATTGG AGGCCTGGGA GCCGCCCGAG TGCGCAGCCT CTTCAAGGAA 
GCCCGGGCCA GGGCCCCTTG CATTGTGTAC ATTGATGAGA TCGATGCTGT GGGGAAGAAG 
CGCTCCACCT CCATGTCCGG GTTCTCCAAC ACAGAAGAGG AGCAGACCCT CAACCAGCTC 
CTCGTAGAGA TGGACGGAAT GGGCACCGCA GACCACGTCA TCGTCTTGGC ATCCACCAAC 
CGAGCCGATG TCCTGGACAA CGCTCTGATG AGGCCCGGTC GGCTCGACAG GCACGTCTTC 
ATCGATCTTC CCACTCTCCA GGAGAGGCGG GAGATTTTCG AGCAGCACCT GAAAGGCCTC 
AAGTTGACCC AGCCCAGCAG TTTTTACTCC CAGCGGCTGG CAGAGCTGAC TCCTGGATTC 
AGTGGCGCCG ACATCGCCAA CATCTGCAAC GAGGCTGCAC TGCACGCTGC ACGCGAGGGG 
CACACATCCG TCCACACGTT CAACTTCGAG TATGCTGTGG AGCGCGTCAT TGCTGGAACT 
GCTAAAAAGA GTAAGATCCT TTCCAAGGAG GAGCAGAGAG TGGTTGCTTT CCATGAGTCT 
GGCCATGCCC TGGTCGGTTG GCTGCTGGAA CACACGGAGG CTGTGATGAA GGTCTCCATA 
GCACCTCGGA CAAACGCTGC TCTGGGCTTC TCTCAGATGC TCCCTCGGGA CCAGTACCTC 
TTCACCAAGG AGCAGCTGTT TGAGCGGATG TGCATGGCCC TAGGCGGCCG TGCAGCTGAA 
GCCATCTCAT TCAGCAGGGT CACCTCTGGG GCCCAGGATG ATTTGAGGAA GGTTACCCGT 
ATCGCCTACT CCATGGTGAA ACAGTTTGGG ATGGCTCCTA GCATTGGGCC CGTATCCTTC 
CCTGAGGCAC AAGAGGGCCT CGTGGGCATT GGACGCCGTC CCTTCAGCCA GGGTCTCCAG 
CAGATGATGG ACCATGAAGC AAGACTGCTG GTGGCCAGAG CCTACAGACA CACTGAGAAG 
GTGCTGCTGG ACAACCTGGA CAAGCTGCAG GCGCTGGCGA ATGCCCTTCT GGAAAAGGAA 
GTGATAAACT ATGAGGACAT TGAGGCGCTC ATTGGCCCGC CGCCCCATGG GCCAAAGAAA 
ATGATTGCAC CACAGAAATG GATTGATGCT GAAAAGGAGA AACAAGCCTC AGGGGAGGAG 
GAGGCTCCGG CTCCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008770843.1
CDS	361..2298
Translation	MYRERLRTLFIIAIVMSLLNSLSTSGGSISWADFVNEMLAKGEVQRVQVVPESDVVEVYLHPGAVVFGRPRLALMYRMQVANIDKFEEKLRAAEDELNIESKDRIPVSYKRTGFFGNALYALGMTAVGLAILWYVFRLAGMTGREGGFSAFNQLKMARFTIVDGKTGKGVSFQDVAGMHEAKLEVREFVDYLKSPERFLQLGAKVPKGALLLGPPGCGKTLLAKAVATEAQVPFLAMAGPEFVEVIGGLGAARVRSLFKEARARAPCIVYIDEIDAVGKKRSTSMSGFSNTEEEQTLNQLLVEMDGMGTADHVIVLASTNRADVLDNALMRPGRLDRHVFIDLPTLQERREIFEQHLKGLKLTQPSSFYSQRLAELTPGFSGADIANICNEAALHAAREGHTSVHTFNFEYAVERVIAGTAKKSKILSKEEQRVVAFHESGHALVGWLLEHTEAVMKVSIAPRTNAALGFSQMLPRDQYLFTKEQLFERMCMALGGRAAEAISFSRVTSGAQDDLRKVTRIAYSMVKQFGMAPSIGPVSFPEAQEGLVGIGRRPFSQGLQQMMDHEARLLVARAYRHTEKVLLDNLDKLQALANALLEKEVINYEDIEALIGPPPHGPKKMIAPQKWIDAEKEKQASGEEEAPAP

Target ORF information:

RefSeq Version	XM_008772621.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus SPG7, paraplegin matrix AAA peptidase subunit (Spg7), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008772621.2

ORF Insert Sequence:

ATGTACCGGG AGAGGCTGCG CACCTTGTTC ATCATCGCCA TCGTGATGAG CCTGCTCAAC 
TCCCTCAGCA CAAGTGGCGG CAGCATCTCC TGGGCTGACT TCGTCAACGA GATGCTGGCT 
AAAGGCGAGG TGCAGCGTGT GCAGGTGGTG CCCGAGAGTG ATGTGGTGGA AGTCTACCTG 
CATCCAGGAG CTGTGGTGTT TGGGCGGCCT CGGCTGGCCT TGATGTACCG GATGCAGGTT 
GCAAACATCG ACAAATTTGA AGAGAAGCTT CGAGCTGCTG AAGATGAACT GAACATCGAG 
AGCAAGGACA GGATTCCCGT GTCCTACAAG CGGACAGGAT TCTTTGGAAA TGCCCTCTAT 
GCCCTGGGGA TGACTGCAGT GGGCTTGGCC ATCCTGTGGT ATGTTTTCAG ACTGGCTGGG 
ATGACCGGAA GGGAAGGCGG ATTCAGTGCC TTTAATCAGC TTAAAATGGC GCGCTTCACC 
ATCGTGGACG GGAAGACGGG GAAAGGAGTC AGCTTCCAGG ATGTGGCGGG AATGCACGAA 
GCCAAGCTGG AAGTCCGAGA GTTTGTGGAT TATCTGAAGA GCCCGGAGCG TTTCCTTCAG 
CTGGGTGCCA AGGTTCCAAA GGGTGCCCTG CTGCTGGGAC CCCCTGGTTG TGGGAAGACG 
CTGTTGGCCA AGGCAGTGGC CACGGAGGCT CAGGTGCCCT TTTTAGCAAT GGCTGGCCCA 
GAGTTTGTGG AGGTCATTGG AGGCCTGGGA GCCGCCCGAG TGCGCAGCCT CTTCAAGGAA 
GCCCGGGCCA GGGCCCCTTG CATTGTGTAC ATTGATGAGA TCGATGCTGT GGGGAAGAAG 
CGCTCCACCT CCATGTCCGG GTTCTCCAAC ACAGAAGAGG AGCAGACCCT CAACCAGCTC 
CTCGTAGAGA TGGACGGAAT GGGCACCGCA GACCACGTCA TCGTCTTGGC ATCCACCAAC 
CGAGCCGATG TCCTGGACAA CGCTCTGATG AGGCCCGGTC GGCTCGACAG GCACGTCTTC 
ATCGATCTTC CCACTCTCCA GGAGAGGCGG GAGATTTTCG AGCAGCACCT GAAAGGCCTC 
AAGTTGACCC AGCCCAGCAG TTTTTACTCC CAGCGGCTGG CAGAGCTGAC TCCTGGATTC 
AGTGGCGCCG ACATCGCCAA CATCTGCAAC GAGGCTGCAC TGCACGCTGC ACGCGAGGGG 
CACACATCCG TCCACACGTT CAACTTCGAG TATGCTGTGG AGCGCGTCAT TGCTGGAACT 
GCTAAAAAGA GTAAGATCCT TTCCAAGGAG GAGCAGAGAG TGGTTGCTTT CCATGAGTCT 
GGCCATGCCC TGGTCGGTTG GCTGCTGGAA CACACGGAGG CTGTGATGAA GGTCTCCATA 
GCACCTCGGA CAAACGCTGC TCTGGGCTTC TCTCAGATGC TCCCTCGGGA CCAGTACCTC 
TTCACCAAGG AGCAGCTGTT TGAGCGGATG TGCATGGCCC TAGGCGGCCG TGCAGCTGAA 
GCCATCTCAT TCAGCAGGGT CACCTCTGGG GCCCAGGATG ATTTGAGGAA GGTTACCCGT 
ATCGCCTACT CCATGGTGAA ACAGTTTGGG ATGGCTCCTA GCATTGGGCC CGTATCCTTC 
CCTGAGGCAC AAGAGGGCCT CGTGGGCATT GGACGCCGTC CCTTCAGCCA GGGTCTCCAG 
CAGATGATGG ACCATGAAGC AAGACTGCTG GTGGCCAGAG CCTACAGACA CACTGAGAAG 
GTGCTGCTGG ACAACCTGGA CAAGCTGCAG GCGCTGGCGA ATGCCCTTCT GGAAAAGGAA 
GTGATAAACT ATGAGGACAT TGAGGCGCTC ATTGGCCCGC CGCCCCATGG GCCAAAGAAA 
ATGATTGCAC CACAGAAATG GATTGATGCT GAAAAGGAGA AACAAGCCTC AGGGGAGGAG 
GAGGCTCCGG CTCCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spg7 ( NM_181388.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_852053.1 Rattus norvegicus SPG7 matrix AAA peptidase subunit, paraplegin (Spg7), mRNA.

Price & Availability↑

CloneID	ORa12810
Clone ID Related Accession (Same CDS sequence)	NM_181388.2
Accession Version	NM_181388.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2235bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Rattus norvegicus(Norway rat)
Product	paraplegin
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY278739.1. On Jun 3, 2003 this sequence version replaced NM_181388.1. ##Evidence-Data-START## Transcript exon combination :: AY278739.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGGCGGCCG CGTTGCTGCT GCTTCGCGCG CTCCGCCAGA GCCCGGAGCC CGGGCCCTGG 
CGGCTGTGGG CCCAGCTCTC AGGCAGGAGC CCCGGCCTCT TCTCCGGGGC CGGCGGTAGG 
CGGCCGTACG TAGTCCGTGG GACTCCGATT GGCCTGGCTG CGGCTGGAGG CCACACGCCT 
CAGAGCTTGC TATTGCGAAT ACTGACCCCT AGCTTTGAAG GTGTTAGCGG ATTGTTACTG 
AAACGGCATA TAGTTCCAAG TGCAATCAGA CTGTGGCAGC TTTCAGGTAG TACTTTGTAT 
TTTAACACCT CAGGGTTGAA GCAGAAGAAC AAAGATGATG ATAAACCCAA AGGCAAAGCC 
CCGGAAGATG ACGAAGAAGA GAGGAGGCGG AAGGAGCGGG AAGACCAGAT GTACCGGGAG 
AGGCTGCGCA CCTTGTTCAT CATCGCCATC GTGATGAGCC TGCTCAACTC CCTCAGCACA 
AGTGGCGGCA GCATCTCCTG GGCTGACTTC GTCAACGAGA TGCTGGCTAA AGGCGAGGTG 
CAGCGTGTGC AGGTGGTGCC CGAGAGTGAT GTGGTGGAAG TCTACCTGCA TCCAGGAGCT 
GTGGTGTTTG GGCGGCCTCG GCTGGCCTTG ATGTACCGGA TGCAGGTTGC AAACATCGAC 
AAATTTGAAG AGAAGCTTCG AGCTGCTGAA GATGAACTGA ACATCGAGAG CAAGGACAGG 
ATTCCCGTGT CCTACAAGCG GACAGGATTC TTTGGAAATG CCCTCTATGC CCTGGGGATG 
ACTGCAGTGG GCTTGGCCAT CCTGTGGTAT GTTTTCAGAC TGGCTGGGAT GACCGGAAGG 
GAAGGCGGAT TCAGTGCCTT TAATCAGCTT AAAATGGCGC GCTTCACCAT CGTGGACGGG 
AAGACGGGGA AAGGAGTCAG CTTCCAGGAT GTGGCGGGAA TGCACGAAGC CAAGCTGGAA 
GTCCGAGAGT TTGTGGATTA TCTGAAGAGC CCGGAGCGTT TCCTTCAGCT GGGTGCCAAG 
GTTCCAAAGG GTGCCCTGCT GCTGGGACCC CCTGGTTGTG GGAAGACGCT GTTGGCCAAG 
GCAGTGGCCA CGGAGGCTCA GGTGCCCTTT TTAGCAATGG CTGGCCCAGA GTTTGTGGAG 
GTCATTGGAG GCCTGGGAGC CGCCCGAGTG CGCAGCCTCT TCAAGGAAGC CCGGGCCAGG 
GCCCCTTGCA TTGTGTACAT TGATGAGATC GATGCTGTGG GGAAGAAGCG CTCCACCTCC 
ATGTCCGGGT TCTCCAACAC AGAAGAGGAG CAGACCCTCA ACCAGCTCCT CGTAGAGATG 
GACGGAATGG GCACCGCAGA CCACGTCATC GTCTTGGCAT CCACCAACCG AGCCGATGTC 
CTGGACAACG CTCTGATGAG GCCCGGTCGG CTCGACAGGC ACGTCTTCAT CGATCTTCCC 
ACTCTCCAGG AGAGGCGGGA GATTTTCGAG CAGCACCTGA AAGGCCTCAA GTTGACCCAG 
CCCAGCAGTT TTTACTCCCA GCGGCTGGCA GAGCTGACTC CTGGATTCAG TGGAACTGCT 
AAAAAGAGTA AGATCCTTTC CAAGGAGGAG CAGAGAGTGG TTGCTTTCCA TGAGTCTGGC 
CATGCCCTGG TCGGTTGGCT GCTGGAACAC ACGGAGGCTG TGATGAAGGT CTCCATAGCA 
CCTCGGACAA ACGCTGCTCT GGGCTTCTCT CAGATGCTCC CTCGGGACCA GTACCTCTTC 
ACCAAGGAGC AGCTGTTTGA GCGGATGTGC ATGGCCCTAG GCGGCCGTGC AGCTGAAGCC 
ATCTCATTCA GCAGGGTCAC CTCTGGGGCC CAGGATGATT TGAGGAAGGT TACCCGTATC 
GCCTACTCCA TGGTGAAACA GTTTGGGATG GCTCCTAGCA TTGGGCCCGT ATCCTTCCCT 
GAGGCACAAG AGGGCCTCGT GGGCATTGGA CGCCGTCCCT TCAGCCAGGG TCTCCAGCAG 
ATGATGGACC ATGAAGCAAG ACTGCTGGTG GCCAGAGCCT ACAGACACAC TGAGAAGGTG 
CTGCTGGACA ACCTGGACAA GCTGCAGGCG CTGGCGAATG CCCTTCTGGA AAAGGAAGTG 
ATAAACTATG AGGACATTGA GGCGCTCATT GGCCCGCCGC CCCATGGGCC AAAGAAAATG 
ATTGCACCAC AGAAATGGAT TGATGCTGAA AAGGAGAAAC AAGCCTCAGG GGAGGAGGAG 
GCTCCGGCTC CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_852053.1
CDS	52..2286
Translation	MAAALLLLRALRQSPEPGPWRLWAQLSGRSPGLFSGAGGRRPYVVRGTPIGLAAAGGHTPQSLLLRILTPSFEGVSGLLLKRHIVPSAIRLWQLSGSTLYFNTSGLKQKNKDDDKPKGKAPEDDEEERRRKEREDQMYRERLRTLFIIAIVMSLLNSLSTSGGSISWADFVNEMLAKGEVQRVQVVPESDVVEVYLHPGAVVFGRPRLALMYRMQVANIDKFEEKLRAAEDELNIESKDRIPVSYKRTGFFGNALYALGMTAVGLAILWYVFRLAGMTGREGGFSAFNQLKMARFTIVDGKTGKGVSFQDVAGMHEAKLEVREFVDYLKSPERFLQLGAKVPKGALLLGPPGCGKTLLAKAVATEAQVPFLAMAGPEFVEVIGGLGAARVRSLFKEARARAPCIVYIDEIDAVGKKRSTSMSGFSNTEEEQTLNQLLVEMDGMGTADHVIVLASTNRADVLDNALMRPGRLDRHVFIDLPTLQERREIFEQHLKGLKLTQPSSFYSQRLAELTPGFSGTAKKSKILSKEEQRVVAFHESGHALVGWLLEHTEAVMKVSIAPRTNAALGFSQMLPRDQYLFTKEQLFERMCMALGGRAAEAISFSRVTSGAQDDLRKVTRIAYSMVKQFGMAPSIGPVSFPEAQEGLVGIGRRPFSQGLQQMMDHEARLLVARAYRHTEKVLLDNLDKLQALANALLEKEVINYEDIEALIGPPPHGPKKMIAPQKWIDAEKEKQASGEEEAPAP

Target ORF information:

RefSeq Version	NM_181388.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus SPG7 matrix AAA peptidase subunit, paraplegin (Spg7), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_181388.2

ORF Insert Sequence:

ATGGCGGCCG CGTTGCTGCT GCTTCGCGCG CTCCGCCAGA GCCCGGAGCC CGGGCCCTGG 
CGGCTGTGGG CCCAGCTCTC AGGCAGGAGC CCCGGCCTCT TCTCCGGGGC CGGCGGTAGG 
CGGCCGTACG TAGTCCGTGG GACTCCGATT GGCCTGGCTG CGGCTGGAGG CCACACGCCT 
CAGAGCTTGC TATTGCGAAT ACTGACCCCT AGCTTTGAAG GTGTTAGCGG ATTGTTACTG 
AAACGGCATA TAGTTCCAAG TGCAATCAGA CTGTGGCAGC TTTCAGGTAG TACTTTGTAT 
TTTAACACCT CAGGGTTGAA GCAGAAGAAC AAAGATGATG ATAAACCCAA AGGCAAAGCC 
CCGGAAGATG ACGAAGAAGA GAGGAGGCGG AAGGAGCGGG AAGACCAGAT GTACCGGGAG 
AGGCTGCGCA CCTTGTTCAT CATCGCCATC GTGATGAGCC TGCTCAACTC CCTCAGCACA 
AGTGGCGGCA GCATCTCCTG GGCTGACTTC GTCAACGAGA TGCTGGCTAA AGGCGAGGTG 
CAGCGTGTGC AGGTGGTGCC CGAGAGTGAT GTGGTGGAAG TCTACCTGCA TCCAGGAGCT 
GTGGTGTTTG GGCGGCCTCG GCTGGCCTTG ATGTACCGGA TGCAGGTTGC AAACATCGAC 
AAATTTGAAG AGAAGCTTCG AGCTGCTGAA GATGAACTGA ACATCGAGAG CAAGGACAGG 
ATTCCCGTGT CCTACAAGCG GACAGGATTC TTTGGAAATG CCCTCTATGC CCTGGGGATG 
ACTGCAGTGG GCTTGGCCAT CCTGTGGTAT GTTTTCAGAC TGGCTGGGAT GACCGGAAGG 
GAAGGCGGAT TCAGTGCCTT TAATCAGCTT AAAATGGCGC GCTTCACCAT CGTGGACGGG 
AAGACGGGGA AAGGAGTCAG CTTCCAGGAT GTGGCGGGAA TGCACGAAGC CAAGCTGGAA 
GTCCGAGAGT TTGTGGATTA TCTGAAGAGC CCGGAGCGTT TCCTTCAGCT GGGTGCCAAG 
GTTCCAAAGG GTGCCCTGCT GCTGGGACCC CCTGGTTGTG GGAAGACGCT GTTGGCCAAG 
GCAGTGGCCA CGGAGGCTCA GGTGCCCTTT TTAGCAATGG CTGGCCCAGA GTTTGTGGAG 
GTCATTGGAG GCCTGGGAGC CGCCCGAGTG CGCAGCCTCT TCAAGGAAGC CCGGGCCAGG 
GCCCCTTGCA TTGTGTACAT TGATGAGATC GATGCTGTGG GGAAGAAGCG CTCCACCTCC 
ATGTCCGGGT TCTCCAACAC AGAAGAGGAG CAGACCCTCA ACCAGCTCCT CGTAGAGATG 
GACGGAATGG GCACCGCAGA CCACGTCATC GTCTTGGCAT CCACCAACCG AGCCGATGTC 
CTGGACAACG CTCTGATGAG GCCCGGTCGG CTCGACAGGC ACGTCTTCAT CGATCTTCCC 
ACTCTCCAGG AGAGGCGGGA GATTTTCGAG CAGCACCTGA AAGGCCTCAA GTTGACCCAG 
CCCAGCAGTT TTTACTCCCA GCGGCTGGCA GAGCTGACTC CTGGATTCAG TGGAACTGCT 
AAAAAGAGTA AGATCCTTTC CAAGGAGGAG CAGAGAGTGG TTGCTTTCCA TGAGTCTGGC 
CATGCCCTGG TCGGTTGGCT GCTGGAACAC ACGGAGGCTG TGATGAAGGT CTCCATAGCA 
CCTCGGACAA ACGCTGCTCT GGGCTTCTCT CAGATGCTCC CTCGGGACCA GTACCTCTTC 
ACCAAGGAGC AGCTGTTTGA GCGGATGTGC ATGGCCCTAG GCGGCCGTGC AGCTGAAGCC 
ATCTCATTCA GCAGGGTCAC CTCTGGGGCC CAGGATGATT TGAGGAAGGT TACCCGTATC 
GCCTACTCCA TGGTGAAACA GTTTGGGATG GCTCCTAGCA TTGGGCCCGT ATCCTTCCCT 
GAGGCACAAG AGGGCCTCGT GGGCATTGGA CGCCGTCCCT TCAGCCAGGG TCTCCAGCAG 
ATGATGGACC ATGAAGCAAG ACTGCTGGTG GCCAGAGCCT ACAGACACAC TGAGAAGGTG 
CTGCTGGACA ACCTGGACAA GCTGCAGGCG CTGGCGAATG CCCTTCTGGA AAAGGAAGTG 
ATAAACTATG AGGACATTGA GGCGCTCATT GGCCCGCCGC CCCATGGGCC AAAGAAAATG 
ATTGCACCAC AGAAATGGAT TGATGCTGAA AAGGAGAAAC AAGCCTCAGG GGAGGAGGAG 
GCTCCGGCTC CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spg7 ( XM_008772619.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008770841.1 Rattus norvegicus SPG7, paraplegin matrix AAA peptidase subunit (Spg7), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa47645
Clone ID Related Accession (Same CDS sequence)	XM_008772619.2
Accession Version	XM_008772619.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2346bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	paraplegin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005118.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008772619.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCCG CGTTGCTGCT GCTTCGCGCG CTCCGCCAGA GCCCGGAGCC CGGGCCCTGG 
CGGCTGTGGG CCCAGCTCTC AGGCAGGAGC CCCGGCCTCT TCTCCGGGGC CGGCGGTAGG 
CGGCCGTACG TAGTCCGTGG GACTCCGATT GGCCTGGCTG CGGCTGGAGG CCACACGCCT 
CAGAGCTTGC TATTGCGAAT ACTGACCCCT AGCTTTGAAG GTGTTAGCGG ATTGTTACTG 
AAACGGCATA TAGTTCCAAG TGCAATCAGA CTGTGGCAGC TTTCAGGTAG TACTTTGTAT 
TTTAACACCT CAGGGTTGAA GCAGAAGAAC AAAGATGATG ATAAACCCAA AGGCAAAGCC 
CCGGAAGATG ACGAAGAAGA GAGGAGGCGG AAGGAGCGGG AAGACCAGAT GTACCGGGAG 
AGGCTGCGCA CCTTGTTCAT CATCGCCATC GTGATGAGCC TGCTCAACTC CCTCAGCACA 
AGTGGCGGCA GCATCTCCTG GGCTGACTTC GTCAACGAGA TGCTGGCTAA AGGCGAGGTG 
CAGCGTGTGC AGGTGGTGCC CGAGAGTGAT GTGGTGGAAG TCTACCTGCA TCCAGGAGCT 
GTGGTGTTTG GGCGGCCTCG GCTGGCCTTG ATGTACCGGA TGCAGGTTGC AAACATCGAC 
AAATTTGAAG AGAAGCTTCG AGCTGCTGAA GATGAACTGA ACATCGAGAG CAAGGACAGG 
ATTCCCGTGT CCTACAAGCG GACAGGATTC TTTGGAAATG CCCTCTATGC CCTGGGGATG 
ACTGCAGTGG GCTTGGCCAT CCTGTGGTAT GTTTTCAGAC TGGCTGGGAT GACCGGAAGG 
GAAGGCGGAT TCAGTGCCTT TAATCAGCTT AAAATGGCGC GCTTCACCAT CGTGGACGGG 
AAGACGGGGA AAGGAGTCAG CTTCCAGGAT GTGGCGGGAA TGCACGAAGC CAAGCTGGAA 
GTCCGAGAGT TTGTGGATTA TCTGAAGAGC CCGGAGCGTT TCCTTCAGCT GGGTGCCAAG 
GTTCCAAAGG GTGCCCTGCT GCTGGGACCC CCTGGTTGTG GGAAGACGCT GTTGGCCAAG 
GCAGTGGCCA CGGAGGCTCA GGTGCCCTTT TTAGCAATGG CTGGCCCAGA GTTTGTGGAG 
GTCATTGGAG GCCTGGGAGC CGCCCGAGTG CGCAGCCTCT TCAAGGAAGC CCGGGCCAGG 
GCCCCTTGCA TTGTGTACAT TGATGAGATC GATGCTGTGG GGAAGAAGCG CTCCACCTCC 
ATGTCCGGGT TCTCCAACAC AGAAGAGGAG CAGACCCTCA ACCAGCTCCT CGTAGAGATG 
GACGGAATGG GCACCGCAGA CCACGTCATC GTCTTGGCAT CCACCAACCG AGCCGATGTC 
CTGGACAACG CTCTGATGAG GCCCGGTCGG CTCGACAGGC ACGTCTTCAT CGATCTTCCC 
ACTCTCCAGG AGAGGCGGGA GATTTTCGAG CAGCACCTGA AAGGCCTCAA GTTGACCCAG 
CCCAGCAGTT TTTACTCCCA GCGGCTGGCA GAGCTGACTC CTGGATTCAG TGGCGCCGAC 
ATCGCCAACA TCTGCAACGA GGCTGCACTG CACGCTGCAC GCGAGGGGCA CACATCCGTC 
CACACGTTCA ACTTCGAGTA TGCTGTGGAG CGCGTCATTG CTGGAACTGC TAAAAAGAGT 
AAGATCCTTT CCAAGGAGGA GCAGAGAGTG GTTGCTTTCC ATGAGTCTGG CCATGCCCTG 
GTCGGTTGGC TGCTGGAACA CACGGAGGCT GTGATGAAGG TCTCCATAGC ACCTCGGACA 
AACGCTGCTC TGGGCTTCTC TCAGATGCTC CCTCGGGACC AGTACCTCTT CACCAAGGAG 
CAGCTGTTTG AGCGGATGTG CATGGCCCTA GGCGGCCGTG CAGCTGAAGC CATCTCATTC 
AGCAGGGTCA CCTCTGGGGC CCAGGATGAT TTGAGGAAGG TTACCCGTAT CGCCTACTCC 
ATGGTGAAAC AGTTTGGGAT GGCTCCTAGC ATTGGGCCCG TATCCTTCCC TGAGGCACAA 
GAGGGCCTCG TGGGCATTGG ACGCCGTCCC TTCAGCCAGG GTCTCCAGCA GATGATGGAC 
CATGAAGCAA GACTGCTGGT GGCCAGAGCC TACAGACACA CTGAGAAGGT GCTGCTGGAC 
AACCTGGACA AGCTGCAGGC GCTGGCGAAT GCCCTTCTGG AAAAGGAAGT GATAAACTAT 
GAGGACATTG AGGCGCTCAT TGGCCCGCCG CCCCATGGGC CAAAGAAAAT GATTGCACCA 
CAGAAATGGA TTGATGCTGA AAAGGAGAAA CAAGCCTCAG GGGAGGAGGA GGCTCCGGCT 
CCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008770841.1
CDS	64..2409
Translation	MAAALLLLRALRQSPEPGPWRLWAQLSGRSPGLFSGAGGRRPYVVRGTPIGLAAAGGHTPQSLLLRILTPSFEGVSGLLLKRHIVPSAIRLWQLSGSTLYFNTSGLKQKNKDDDKPKGKAPEDDEEERRRKEREDQMYRERLRTLFIIAIVMSLLNSLSTSGGSISWADFVNEMLAKGEVQRVQVVPESDVVEVYLHPGAVVFGRPRLALMYRMQVANIDKFEEKLRAAEDELNIESKDRIPVSYKRTGFFGNALYALGMTAVGLAILWYVFRLAGMTGREGGFSAFNQLKMARFTIVDGKTGKGVSFQDVAGMHEAKLEVREFVDYLKSPERFLQLGAKVPKGALLLGPPGCGKTLLAKAVATEAQVPFLAMAGPEFVEVIGGLGAARVRSLFKEARARAPCIVYIDEIDAVGKKRSTSMSGFSNTEEEQTLNQLLVEMDGMGTADHVIVLASTNRADVLDNALMRPGRLDRHVFIDLPTLQERREIFEQHLKGLKLTQPSSFYSQRLAELTPGFSGADIANICNEAALHAAREGHTSVHTFNFEYAVERVIAGTAKKSKILSKEEQRVVAFHESGHALVGWLLEHTEAVMKVSIAPRTNAALGFSQMLPRDQYLFTKEQLFERMCMALGGRAAEAISFSRVTSGAQDDLRKVTRIAYSMVKQFGMAPSIGPVSFPEAQEGLVGIGRRPFSQGLQQMMDHEARLLVARAYRHTEKVLLDNLDKLQALANALLEKEVINYEDIEALIGPPPHGPKKMIAPQKWIDAEKEKQASGEEEAPAP

Target ORF information:

RefSeq Version	XM_008772619.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus SPG7, paraplegin matrix AAA peptidase subunit (Spg7), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008772619.2

ORF Insert Sequence:

ATGGCGGCCG CGTTGCTGCT GCTTCGCGCG CTCCGCCAGA GCCCGGAGCC CGGGCCCTGG 
CGGCTGTGGG CCCAGCTCTC AGGCAGGAGC CCCGGCCTCT TCTCCGGGGC CGGCGGTAGG 
CGGCCGTACG TAGTCCGTGG GACTCCGATT GGCCTGGCTG CGGCTGGAGG CCACACGCCT 
CAGAGCTTGC TATTGCGAAT ACTGACCCCT AGCTTTGAAG GTGTTAGCGG ATTGTTACTG 
AAACGGCATA TAGTTCCAAG TGCAATCAGA CTGTGGCAGC TTTCAGGTAG TACTTTGTAT 
TTTAACACCT CAGGGTTGAA GCAGAAGAAC AAAGATGATG ATAAACCCAA AGGCAAAGCC 
CCGGAAGATG ACGAAGAAGA GAGGAGGCGG AAGGAGCGGG AAGACCAGAT GTACCGGGAG 
AGGCTGCGCA CCTTGTTCAT CATCGCCATC GTGATGAGCC TGCTCAACTC CCTCAGCACA 
AGTGGCGGCA GCATCTCCTG GGCTGACTTC GTCAACGAGA TGCTGGCTAA AGGCGAGGTG 
CAGCGTGTGC AGGTGGTGCC CGAGAGTGAT GTGGTGGAAG TCTACCTGCA TCCAGGAGCT 
GTGGTGTTTG GGCGGCCTCG GCTGGCCTTG ATGTACCGGA TGCAGGTTGC AAACATCGAC 
AAATTTGAAG AGAAGCTTCG AGCTGCTGAA GATGAACTGA ACATCGAGAG CAAGGACAGG 
ATTCCCGTGT CCTACAAGCG GACAGGATTC TTTGGAAATG CCCTCTATGC CCTGGGGATG 
ACTGCAGTGG GCTTGGCCAT CCTGTGGTAT GTTTTCAGAC TGGCTGGGAT GACCGGAAGG 
GAAGGCGGAT TCAGTGCCTT TAATCAGCTT AAAATGGCGC GCTTCACCAT CGTGGACGGG 
AAGACGGGGA AAGGAGTCAG CTTCCAGGAT GTGGCGGGAA TGCACGAAGC CAAGCTGGAA 
GTCCGAGAGT TTGTGGATTA TCTGAAGAGC CCGGAGCGTT TCCTTCAGCT GGGTGCCAAG 
GTTCCAAAGG GTGCCCTGCT GCTGGGACCC CCTGGTTGTG GGAAGACGCT GTTGGCCAAG 
GCAGTGGCCA CGGAGGCTCA GGTGCCCTTT TTAGCAATGG CTGGCCCAGA GTTTGTGGAG 
GTCATTGGAG GCCTGGGAGC CGCCCGAGTG CGCAGCCTCT TCAAGGAAGC CCGGGCCAGG 
GCCCCTTGCA TTGTGTACAT TGATGAGATC GATGCTGTGG GGAAGAAGCG CTCCACCTCC 
ATGTCCGGGT TCTCCAACAC AGAAGAGGAG CAGACCCTCA ACCAGCTCCT CGTAGAGATG 
GACGGAATGG GCACCGCAGA CCACGTCATC GTCTTGGCAT CCACCAACCG AGCCGATGTC 
CTGGACAACG CTCTGATGAG GCCCGGTCGG CTCGACAGGC ACGTCTTCAT CGATCTTCCC 
ACTCTCCAGG AGAGGCGGGA GATTTTCGAG CAGCACCTGA AAGGCCTCAA GTTGACCCAG 
CCCAGCAGTT TTTACTCCCA GCGGCTGGCA GAGCTGACTC CTGGATTCAG TGGCGCCGAC 
ATCGCCAACA TCTGCAACGA GGCTGCACTG CACGCTGCAC GCGAGGGGCA CACATCCGTC 
CACACGTTCA ACTTCGAGTA TGCTGTGGAG CGCGTCATTG CTGGAACTGC TAAAAAGAGT 
AAGATCCTTT CCAAGGAGGA GCAGAGAGTG GTTGCTTTCC ATGAGTCTGG CCATGCCCTG 
GTCGGTTGGC TGCTGGAACA CACGGAGGCT GTGATGAAGG TCTCCATAGC ACCTCGGACA 
AACGCTGCTC TGGGCTTCTC TCAGATGCTC CCTCGGGACC AGTACCTCTT CACCAAGGAG 
CAGCTGTTTG AGCGGATGTG CATGGCCCTA GGCGGCCGTG CAGCTGAAGC CATCTCATTC 
AGCAGGGTCA CCTCTGGGGC CCAGGATGAT TTGAGGAAGG TTACCCGTAT CGCCTACTCC 
ATGGTGAAAC AGTTTGGGAT GGCTCCTAGC ATTGGGCCCG TATCCTTCCC TGAGGCACAA 
GAGGGCCTCG TGGGCATTGG ACGCCGTCCC TTCAGCCAGG GTCTCCAGCA GATGATGGAC 
CATGAAGCAA GACTGCTGGT GGCCAGAGCC TACAGACACA CTGAGAAGGT GCTGCTGGAC 
AACCTGGACA AGCTGCAGGC GCTGGCGAAT GCCCTTCTGG AAAAGGAAGT GATAAACTAT 
GAGGACATTG AGGCGCTCAT TGGCCCGCCG CCCCATGGGC CAAAGAAAAT GATTGCACCA 
CAGAAATGGA TTGATGCTGA AAAGGAGAAA CAAGCCTCAG GGGAGGAGGA GGCTCCGGCT 
CCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spg7 ( XM_017601307.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017456796.1 Rattus norvegicus SPG7, paraplegin matrix AAA peptidase subunit (Spg7), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa59450
Clone ID Related Accession (Same CDS sequence)	XM_017601307.1
Accession Version	XM_017601307.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1977bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	paraplegin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005118.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCCG CGTTGCTGCT GCTTCGCGCG CTCCGCCAGA GCCCGGAGCC CGGGCCCTGG 
CGGCTGTGGG CCCAGCTCTC AGGCAGGAGC CCCGGCCTCT TCTCCGGGGC CGGCGGTAGG 
CGGCCGTACG TAGTCCGTGG GACTCCGATT GGCCTGGCTG CGGCTGGAGG CCACACGCCT 
CAGAGCTTGC TATTGCGAAT ACTGACCCCT AGCTTTGAAG GTGTTAGCGG ATTGTTACTG 
AAACGGCATA TAGTTCCAAG TGCAATCAGA CTGTGGCAGC TTTCAGGTAG TACTTTGTAT 
TTTAACACCT CAGGGTTGAA GCAGAAGAAC AAAGATGATG ATAAACCCAA AGGCAAAGCC 
CCGGAAGATG ACGAAGAAGA GAGGAGGCGG AAGGAGCGGG AAGACCAGAT GTACCGGGAG 
AGGCTGCGCA CCTTGTTCAT CATCGCCATC GTGATGAGCC TGCTCAACTC CCTCAGCACA 
AGTGGCGGCA GCATCTCCTG GGCTGACTTC GTCAACGAGA TGCTGGCTAA AGGCGAGGTG 
CAGCGTGTGC AGGTGGTGCC CGAGAGTGAT GTGGTGGAAG TCTACCTGCA TCCAGGAGCT 
GTGGTGTTTG GGCGGCCTCG GCTGGCCTTG ATGTACCGGA TGCAGGTTGC AAACATCGAC 
AAATTTGAAG AGAAGCTTCG AGCTGCTGAA GATGAACTGA ACATCGAGAG CAAGGACAGG 
ATTCCCGTGT CCTACAAGCG GACAGGATTC TTTGGAAATG CCCTCTATGC CCTGGGGATG 
ACTGCAGTGG GCTTGGCCAT CCTGTGGTAT GTTTTCAGAC TGGCTGGGAT GACCGGAAGG 
GAAGGCGGAT TCAGTGCCTT TAATCAGCTT AAAATGGCGC GCTTCACCAT CGTGGACGGG 
AAGACGGGGA AAGGAGTCAG CTTCCAGGAT GTGGCGGGAA TGCACGAAGC CAAGCTGGAA 
GTCCGAGAGT TTGTGGATTA TCTGAAGAGC CCGGAGCGTT TCCTTCAGCT GGGTGCCAAG 
GTTCCAAAGG GTGCCCTGCT GCTGGGACCC CCTGGTTGTG GGAAGACGCT GTTGGCCAAG 
GCAGTGGCCA CGGAGGCTCA GGTGCCCTTT TTAGCAATGG CTGGCCCAGA GTTTGTGGAG 
GTCATTGGAG GCCTGGGAGC CGCCCGAGTG CGCAGCCTCT TCAAGGAAGC CCGGGCCAGG 
GCCCCTTGCA TTGTGTACAT TGATGAGATC GATGCTGTGG GGAAGAAGCG CTCCACCTCC 
ATGTCCGGGT TCTCCAACAC AGAAGAGGAG CAGACCCTCA ACCAGCTCCT CGTAGAGATG 
GACGGAATGG GCACCGCAGA CCACGTCATC GTCTTGGCAT CCACCAACCG AGCCGATGTC 
CTGGACAACG CTCTGATGAG GCCCGGTCGG CTCGACAGGC ACGTCTTCAT CGATCTTCCC 
ACTCTCCAGG AGAGGCGGGA GATTTTCGAG CAGCACCTGA AAGGCCTCAA GTTGACCCAG 
CCCAGCAGTT TTTACTCCCA GCGGCTGGCA GAGCTGACTC CTGGATTCAG TGGCGCCGAC 
ATCGCCAACA TCTGCAACGA GGCTGCACTG CACGCTGCAC GCGAGGGGCA CACATCCGTC 
CACACGTTCA ACTTCGAGTA TGCTGTGGAG CGCGTCATTG CTGGAACTGC TAAAAAGAGT 
AAGATCCTTT CCAAGGAGGA GCAGAGAGTG GTTGCTTTCC ATGAGTCTGG CCATGCCCTG 
GTCGGTTGGC TGCTGGAACA CACGGAGGCT GTGATGAAGG TCTCCATAGC ACCTCGGACA 
AACGCTGCTC TGGGCTTCTC TCAGATGCTC CCTCGGGACC AGTACCTCTT CACCAAGGAG 
CAGCTGTTTG AGCGGATGTG CATGGCCCTA GGCGGCCGTG CAGCTGAAGC CATCTCATTC 
AGCAGGGTCA CCTCTGGAAG CAAGACTGCT GGTGGCCAGA GCCTACAGAC ACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017456796.1
CDS	43..2019
Translation	MAAALLLLRALRQSPEPGPWRLWAQLSGRSPGLFSGAGGRRPYVVRGTPIGLAAAGGHTPQSLLLRILTPSFEGVSGLLLKRHIVPSAIRLWQLSGSTLYFNTSGLKQKNKDDDKPKGKAPEDDEEERRRKEREDQMYRERLRTLFIIAIVMSLLNSLSTSGGSISWADFVNEMLAKGEVQRVQVVPESDVVEVYLHPGAVVFGRPRLALMYRMQVANIDKFEEKLRAAEDELNIESKDRIPVSYKRTGFFGNALYALGMTAVGLAILWYVFRLAGMTGREGGFSAFNQLKMARFTIVDGKTGKGVSFQDVAGMHEAKLEVREFVDYLKSPERFLQLGAKVPKGALLLGPPGCGKTLLAKAVATEAQVPFLAMAGPEFVEVIGGLGAARVRSLFKEARARAPCIVYIDEIDAVGKKRSTSMSGFSNTEEEQTLNQLLVEMDGMGTADHVIVLASTNRADVLDNALMRPGRLDRHVFIDLPTLQERREIFEQHLKGLKLTQPSSFYSQRLAELTPGFSGADIANICNEAALHAAREGHTSVHTFNFEYAVERVIAGTAKKSKILSKEEQRVVAFHESGHALVGWLLEHTEAVMKVSIAPRTNAALGFSQMLPRDQYLFTKEQLFERMCMALGGRAAEAISFSRVTSGSKTAGGQSLQTH

Target ORF information:

RefSeq Version	XM_017601307.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus SPG7, paraplegin matrix AAA peptidase subunit (Spg7), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017601307.1

ORF Insert Sequence:

ATGGCGGCCG CGTTGCTGCT GCTTCGCGCG CTCCGCCAGA GCCCGGAGCC CGGGCCCTGG 
CGGCTGTGGG CCCAGCTCTC AGGCAGGAGC CCCGGCCTCT TCTCCGGGGC CGGCGGTAGG 
CGGCCGTACG TAGTCCGTGG GACTCCGATT GGCCTGGCTG CGGCTGGAGG CCACACGCCT 
CAGAGCTTGC TATTGCGAAT ACTGACCCCT AGCTTTGAAG GTGTTAGCGG ATTGTTACTG 
AAACGGCATA TAGTTCCAAG TGCAATCAGA CTGTGGCAGC TTTCAGGTAG TACTTTGTAT 
TTTAACACCT CAGGGTTGAA GCAGAAGAAC AAAGATGATG ATAAACCCAA AGGCAAAGCC 
CCGGAAGATG ACGAAGAAGA GAGGAGGCGG AAGGAGCGGG AAGACCAGAT GTACCGGGAG 
AGGCTGCGCA CCTTGTTCAT CATCGCCATC GTGATGAGCC TGCTCAACTC CCTCAGCACA 
AGTGGCGGCA GCATCTCCTG GGCTGACTTC GTCAACGAGA TGCTGGCTAA AGGCGAGGTG 
CAGCGTGTGC AGGTGGTGCC CGAGAGTGAT GTGGTGGAAG TCTACCTGCA TCCAGGAGCT 
GTGGTGTTTG GGCGGCCTCG GCTGGCCTTG ATGTACCGGA TGCAGGTTGC AAACATCGAC 
AAATTTGAAG AGAAGCTTCG AGCTGCTGAA GATGAACTGA ACATCGAGAG CAAGGACAGG 
ATTCCCGTGT CCTACAAGCG GACAGGATTC TTTGGAAATG CCCTCTATGC CCTGGGGATG 
ACTGCAGTGG GCTTGGCCAT CCTGTGGTAT GTTTTCAGAC TGGCTGGGAT GACCGGAAGG 
GAAGGCGGAT TCAGTGCCTT TAATCAGCTT AAAATGGCGC GCTTCACCAT CGTGGACGGG 
AAGACGGGGA AAGGAGTCAG CTTCCAGGAT GTGGCGGGAA TGCACGAAGC CAAGCTGGAA 
GTCCGAGAGT TTGTGGATTA TCTGAAGAGC CCGGAGCGTT TCCTTCAGCT GGGTGCCAAG 
GTTCCAAAGG GTGCCCTGCT GCTGGGACCC CCTGGTTGTG GGAAGACGCT GTTGGCCAAG 
GCAGTGGCCA CGGAGGCTCA GGTGCCCTTT TTAGCAATGG CTGGCCCAGA GTTTGTGGAG 
GTCATTGGAG GCCTGGGAGC CGCCCGAGTG CGCAGCCTCT TCAAGGAAGC CCGGGCCAGG 
GCCCCTTGCA TTGTGTACAT TGATGAGATC GATGCTGTGG GGAAGAAGCG CTCCACCTCC 
ATGTCCGGGT TCTCCAACAC AGAAGAGGAG CAGACCCTCA ACCAGCTCCT CGTAGAGATG 
GACGGAATGG GCACCGCAGA CCACGTCATC GTCTTGGCAT CCACCAACCG AGCCGATGTC 
CTGGACAACG CTCTGATGAG GCCCGGTCGG CTCGACAGGC ACGTCTTCAT CGATCTTCCC 
ACTCTCCAGG AGAGGCGGGA GATTTTCGAG CAGCACCTGA AAGGCCTCAA GTTGACCCAG 
CCCAGCAGTT TTTACTCCCA GCGGCTGGCA GAGCTGACTC CTGGATTCAG TGGCGCCGAC 
ATCGCCAACA TCTGCAACGA GGCTGCACTG CACGCTGCAC GCGAGGGGCA CACATCCGTC 
CACACGTTCA ACTTCGAGTA TGCTGTGGAG CGCGTCATTG CTGGAACTGC TAAAAAGAGT 
AAGATCCTTT CCAAGGAGGA GCAGAGAGTG GTTGCTTTCC ATGAGTCTGG CCATGCCCTG 
GTCGGTTGGC TGCTGGAACA CACGGAGGCT GTGATGAAGG TCTCCATAGC ACCTCGGACA 
AACGCTGCTC TGGGCTTCTC TCAGATGCTC CCTCGGGACC AGTACCTCTT CACCAAGGAG 
CAGCTGTTTG AGCGGATGTG CATGGCCCTA GGCGGCCGTG CAGCTGAAGC CATCTCATTC 
AGCAGGGTCA CCTCTGGAAG CAAGACTGCT GGTGGCCAGA GCCTACAGAC ACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

SPG7 Is an Essential and Conserved Component of the Mitochondrial Permeability Transition Pore.
Molecular cell60(1)47-62(2015 Oct)
Shanmughapriya S,Rajan S,Hoffman NE,Higgins AM,Tomar D,Nemani N,Hines KJ,Smith DJ,Eguchi A,Vallem S,Shaikh F,Cheung M,Leonard NJ,Stolakis RS,Wolfers MP,Ibetti J,Chuprun JK,Jog NR,Houser SR,Koch WJ,Elrod JW,Madesh M

Autocatalytic processing of m-AAA protease subunits in mitochondria.
Molecular biology of the cell20(19)4216-24(2009 Oct)
Koppen M,Bonn F,Ehses S,Langer T

Axonal degeneration in paraplegin-deficient mice is associated with abnormal mitochondria and impairment of axonal transport.
The Journal of clinical investigation113(2)231-42(2004 Jan)
Ferreirinha F,Quattrini A,Pirozzi M,Valsecchi V,Dina G,Broccoli V,Auricchio A,Piemonte F,Tozzi G,Gaeta L,Casari G,Ballabio A,Rugarli EI

Integrated analysis of protein composition, tissue diversity, and gene regulation in mouse mitochondria.
Cell115(5)629-40(2003 Nov)
Mootha VK,Bunkenborg J,Olsen JV,Hjerrild M,Wisniewski JR,Stahl E,Bolouri MS,Ray HN,Sihag S,Kamal M,Patterson N,Lander ES,Mann M

Molecular and functional analyses of the human and mouse genes encoding AFG3L1, a mitochondrial metalloprotease homologous to the human spastic paraplegia protein.
Genomics76(1-3)58-65(2001 Aug)
Kremmidiotis G,Gardner AE,Settasatian C,Savoia A,Sutherland GR,Callen DF

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Spg7 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Services & Products

見積依頼

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電話

FAX

オンラインフォーム