Opn5 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Opn5
Entrez Gene ID	316259
Full Name	opsin 5
Synonyms	Gpr136
General protein information	Preferred Names opsin 5 Names opsin-5 G protein-coupled receptor 136
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 9 Map Location: 9q13
Summary	G protein-coupled receptor [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_181772.1	NP_861437.1	opsin-5

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Opn5	NP_861437.1
Homo sapiens (human)	OPN5	NP_859528.1
	OPN5	XP_001104044.1
Bos taurus (cattle)	OPN5	NP_001193009.1
Canis lupus familiaris (dog)	OPN5	XP_003639476.1
Mus musculus (house mouse)	Opn5	NP_861418.2
Gallus gallus (chicken)	OPN5	NP_001124215.1
Danio rerio (zebrafish)	opn5	NP_001186975.1
Xenopus tropicalis (tropical clawed frog)	opn5	XP_002936036.1
Pan troglodytes (chimpanzee)	OPN5	XP_001146167.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of mammalian Opn5 as a specialized UV-absorbing pigment by a single amino acid mutation.
Yamashita T, Ono K, Ohuchi H, Yumoto A, Gotoh H, Tomonari S, Sakai K, Fujita H, Imamoto Y, Noji S, Nakamura K, Shichida Y
The Journal of biological chemistry289(7)3991-4000(2014 Feb)

Expression of novel opsins and intrinsic light responses in the mammalian retinal ganglion cell line RGC-5. Presence of OPN5 in the rat retina.
Nieto PS, Valdez DJ, Acosta-Rodr?guez VA, Guido ME
PloS one6(10)e26417(2011)

Seven evolutionarily conserved human rhodopsin G protein-coupled receptors lacking close relatives.
Fredriksson R, H?glund PJ, Gloriam DE, Lagerstr?m MC, Schi?th HB
FEBS letters554(3)381-8(2003 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The presence of Opn5 in the ganglion cell layer of the rat retina suggests the existence of a novel type of photoreceptor cell.
Title: Expression of novel opsins and intrinsic light responses in the mammalian retinal ganglion cell line RGC-5. Presence of OPN5 in the rat retina.

The following Opn5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Opn5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa13410	NM_181772.1 Latest version!	Rattus norvegicus opsin 5 (Opn5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Opn5 ( NM_181772.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_861437.1 Rattus norvegicus opsin 5 (Opn5), mRNA.

Price & Availability↑

CloneID	ORa13410
Clone ID Related Accession (Same CDS sequence)	NM_181772.1
Accession Version	NM_181772.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1605bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-26
Organism	Rattus norvegicus(Norway rat)
Product	opsin-5
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AY288431.1. ##Evidence-Data-START## Transcript exon combination :: AY288431.1 [ECO:0000332] ##Evidence-Data-END##

ATGGCCTTGA ACCACACTGC CCTGCCTCAG GATGAGCGCC TGCCCCACTA TCTTCGAGAC 
GAGGACCCCT TTGCTTCCAA ACTTTCCTGG GAAGCGGATT TGGTGGCTGG CTTTTACCTA 
ACAATAATCG GGATTCTCTC CACATTTGGA AATGGGTATG TCCTTTATAT GTCTTCTAGA 
CGCAAGAAGA AGCTGAGACC TGCGGAAATA ATGACTATTA ATTTAGCAGT CTGTGATCTG 
GGGATTTCAG TTGTAGGCAA GCCATTCACC ATCATCTCTT GCTTCTGCCA CCGCTGGGTG 
TTTGGCTGGT TTGGCTGCCG CTGGTATGGA TGGGCTGGAT TTTTCTTTGG CTGTGGAAGC 
CTGATTACCA TGACTGCTGT CAGCCTGGAC CGCTATCTGA AGATCTGTTA TCTGTCTTAT 
GGGGTCTGGC TGAAGAGAAA GCATGCCTAC ATCTGCCTGG CAGTCATCTG GGCTTATGCT 
TCCTTCTGGA CCACCATGCC CTTGGTGGGC CTGGGGGACT ATGCACCTGA GCCCTTCGGA 
ACCTCGTGCA CCCTGGACTG GTGGCTGGCC CAGGCTTCGG GTGGGGGTCA GGTATTCATC 
CTGAGCATCC TCTTCTTCTG CCTCCTGCTG CCAACGGCTG TGATTGTTTT CTCATACGCG 
AAGATCATTG CCAAGGTGAA ATCTTCTTCT AAAGAGGTAG CACATTTCGA CAGTCGGATT 
CATAGCAGCC ATGTGCTCGA GGTGAAGCTG ACCAAGGGTC TAGTGAGAGC AGATTCAAGC 
CCAGAGTCCT TACCCAGGTC GGTGTTTTCT ACAGCAGAAC CAATCAACTG CCATGTTTTG 
AGTATCTCCC ATTCCAGAGT CTCAACTGTG GCGATGCTGA TTTGTGCTGG TTTCCTCATT 
GCCTGGATTC CTTACGCGGT AGTCTCCGTG TGGTCAGCTT TTGGAAGGCC GAACTCCATC 
CCTATACAGC TCTCCGTGGT GCCCACGCTC CTCGCAAAAT CTGCAGCGAT GTACAACCCA 
ATCATCTACC AGGTCATTGA TTACAGATTT GCCTGTTGCC AGACTGGTGG TTTGAGAGCA 
ACAAAGAAGA AGTCTTTGGA AGACTTTAGT GACTTGAACC AGATTCTGAA GAGGAATAGT 
TCCACTAAGT CAGGGCAAAT AGGAAACAAA GACACAGCTG AGGAGCATGA CCGAGGAACC 
ATGCAGGTGA TAGGGGAGGG AGAGGAAGGA ACAATGGAGT CCACATCCAA CAGTTTAGTG 
CCTTCGTTTC ATTCAGCATT GAAGGGATTT CATTTAACAC AGCTCTTAGC TAACACTTTA 
TCAATCAATA CAAGTAATTT TCGAGCGGTA GAAAAACCAT TCTTAGCACC GAGGCTGCTG 
TCGCTGCTGC TGTACTGGGA TGCTGGTGAA GACGGCCTGA CATTAGCAGA CATGCACCTC 
AAAAATGTTG TTGTTCTCTC AAACAGAGCA GTTCCAGATT TACTAGTGCC CATGTTATGG 
ATGGAGAGAG TCACCGTAAT GACGGTGACT GTGGCAAGAA ATAAGCATCT GTGTTCTGAG 
GCCGAGAAAC CCCGCAGCTG TCCATCAGGG CTTCAGAAAT ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_861437.1
CDS	1..1605
Translation	MALNHTALPQDERLPHYLRDEDPFASKLSWEADLVAGFYLTIIGILSTFGNGYVLYMSSRRKKKLRPAEIMTINLAVCDLGISVVGKPFTIISCFCHRWVFGWFGCRWYGWAGFFFGCGSLITMTAVSLDRYLKICYLSYGVWLKRKHAYICLAVIWAYASFWTTMPLVGLGDYAPEPFGTSCTLDWWLAQASGGGQVFILSILFFCLLLPTAVIVFSYAKIIAKVKSSSKEVAHFDSRIHSSHVLEVKLTKGLVRADSSPESLPRSVFSTAEPINCHVLSISHSRVSTVAMLICAGFLIAWIPYAVVSVWSAFGRPNSIPIQLSVVPTLLAKSAAMYNPIIYQVIDYRFACCQTGGLRATKKKSLEDFSDLNQILKRNSSTKSGQIGNKDTAEEHDRGTMQVIGEGEEGTMESTSNSLVPSFHSALKGFHLTQLLANTLSINTSNFRAVEKPFLAPRLLSLLLYWDAGEDGLTLADMHLKNVVVLSNRAVPDLLVPMLWMERVTVMTVTVARNKHLCSEAEKPRSCPSGLQKY

Target ORF information:

RefSeq Version	NM_181772.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus opsin 5 (Opn5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_181772.1

ORF Insert Sequence:

ATGGCCTTGA ACCACACTGC CCTGCCTCAG GATGAGCGCC TGCCCCACTA TCTTCGAGAC 
GAGGACCCCT TTGCTTCCAA ACTTTCCTGG GAAGCGGATT TGGTGGCTGG CTTTTACCTA 
ACAATAATCG GGATTCTCTC CACATTTGGA AATGGGTATG TCCTTTATAT GTCTTCTAGA 
CGCAAGAAGA AGCTGAGACC TGCGGAAATA ATGACTATTA ATTTAGCAGT CTGTGATCTG 
GGGATTTCAG TTGTAGGCAA GCCATTCACC ATCATCTCTT GCTTCTGCCA CCGCTGGGTG 
TTTGGCTGGT TTGGCTGCCG CTGGTATGGA TGGGCTGGAT TTTTCTTTGG CTGTGGAAGC 
CTGATTACCA TGACTGCTGT CAGCCTGGAC CGCTATCTGA AGATCTGTTA TCTGTCTTAT 
GGGGTCTGGC TGAAGAGAAA GCATGCCTAC ATCTGCCTGG CAGTCATCTG GGCTTATGCT 
TCCTTCTGGA CCACCATGCC CTTGGTGGGC CTGGGGGACT ATGCACCTGA GCCCTTCGGA 
ACCTCGTGCA CCCTGGACTG GTGGCTGGCC CAGGCTTCGG GTGGGGGTCA GGTATTCATC 
CTGAGCATCC TCTTCTTCTG CCTCCTGCTG CCAACGGCTG TGATTGTTTT CTCATACGCG 
AAGATCATTG CCAAGGTGAA ATCTTCTTCT AAAGAGGTAG CACATTTCGA CAGTCGGATT 
CATAGCAGCC ATGTGCTCGA GGTGAAGCTG ACCAAGGGTC TAGTGAGAGC AGATTCAAGC 
CCAGAGTCCT TACCCAGGTC GGTGTTTTCT ACAGCAGAAC CAATCAACTG CCATGTTTTG 
AGTATCTCCC ATTCCAGAGT CTCAACTGTG GCGATGCTGA TTTGTGCTGG TTTCCTCATT 
GCCTGGATTC CTTACGCGGT AGTCTCCGTG TGGTCAGCTT TTGGAAGGCC GAACTCCATC 
CCTATACAGC TCTCCGTGGT GCCCACGCTC CTCGCAAAAT CTGCAGCGAT GTACAACCCA 
ATCATCTACC AGGTCATTGA TTACAGATTT GCCTGTTGCC AGACTGGTGG TTTGAGAGCA 
ACAAAGAAGA AGTCTTTGGA AGACTTTAGT GACTTGAACC AGATTCTGAA GAGGAATAGT 
TCCACTAAGT CAGGGCAAAT AGGAAACAAA GACACAGCTG AGGAGCATGA CCGAGGAACC 
ATGCAGGTGA TAGGGGAGGG AGAGGAAGGA ACAATGGAGT CCACATCCAA CAGTTTAGTG 
CCTTCGTTTC ATTCAGCATT GAAGGGATTT CATTTAACAC AGCTCTTAGC TAACACTTTA 
TCAATCAATA CAAGTAATTT TCGAGCGGTA GAAAAACCAT TCTTAGCACC GAGGCTGCTG 
TCGCTGCTGC TGTACTGGGA TGCTGGTGAA GACGGCCTGA CATTAGCAGA CATGCACCTC 
AAAAATGTTG TTGTTCTCTC AAACAGAGCA GTTCCAGATT TACTAGTGCC CATGTTATGG 
ATGGAGAGAG TCACCGTAAT GACGGTGACT GTGGCAAGAA ATAAGCATCT GTGTTCTGAG 
GCCGAGAAAC CCCGCAGCTG TCCATCAGGG CTTCAGAAAT ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of mammalian Opn5 as a specialized UV-absorbing pigment by a single amino acid mutation.
The Journal of biological chemistry289(7)3991-4000(2014 Feb)
Yamashita T,Ono K,Ohuchi H,Yumoto A,Gotoh H,Tomonari S,Sakai K,Fujita H,Imamoto Y,Noji S,Nakamura K,Shichida Y

Expression of novel opsins and intrinsic light responses in the mammalian retinal ganglion cell line RGC-5. Presence of OPN5 in the rat retina.
PloS one6(10)e26417(2011)
Nieto PS,Valdez DJ,Acosta-Rodr?guez VA,Guido ME

Seven evolutionarily conserved human rhodopsin G protein-coupled receptors lacking close relatives.
FEBS letters554(3)381-8(2003 Nov)
Fredriksson R,H?glund PJ,Gloriam DE,Lagerstr?m MC,Schi?th HB