Manf cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Manf
Entrez Gene ID	315989
Full Name	mesencephalic astrocyte-derived neurotrophic factor
Synonyms	Armet
General protein information	Preferred Names mesencephalic astrocyte-derived neurotrophic factor Names mesencephalic astrocyte-derived neurotrophic factor arginine-rich protein arginine-rich, mutated in early stage tumors
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 8 Map Location: 8q32

mRNA and Protein(s)

mRNA	Protein	Name
XM_006243775.2	XP_006243837.1	mesencephalic astrocyte-derived neurotrophic factor isoform X1
NM_001108183.1	NP_001101653.1	mesencephalic astrocyte-derived neurotrophic factor precursor

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Spatiotemporal expression of MANF in the developing rat brain.
Wang H, Ke Z, Alimov A, Xu M, Frank JA, Fang S, Luo J
PloS one9(2)e90433(2014)

Mesencephalic astrocyte-derived neurotrophic factor (MANF) secretion and cell surface binding are modulated by KDEL receptors.
Henderson MJ, Richie CT, Airavaara M, Wang Y, Harvey BK
The Journal of biological chemistry288(6)4209-25(2013 Feb)

Upregulation of mesencephalic astrocyte-derived neurotrophic factor in glial cells is associated with ischemia-induced glial activation.
Shen Y, Sun A, Wang Y, Cha D, Wang H, Wang F, Feng L, Fang S, Shen Y
Journal of neuroinflammation9254(2012 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

MANF is developmentally regulated and may play a role in the maturation of the central nervous system.
Title: Spatiotemporal expression of MANF in the developing rat brain.

MANF binding to the plasma membrane also required the RTDL sequence and was inhibited with a peptide known to interact with KDELRs, suggesting MANF binds KDELRs at the surface.
Title: Mesencephalic astrocyte-derived neurotrophic factor (MANF) secretion and cell surface binding are modulated by KDEL receptors.

The results of this study indicated that MANF expression was upregulated in the activated glial cells, which may contribute to the mechanism of ischemia-induced neural injury.
Title: Upregulation of mesencephalic astrocyte-derived neurotrophic factor in glial cells is associated with ischemia-induced glial activation.

sarcoplasmic/endoplasmic reticulum calcium depletion triggered MANF secretion by decreasing its retention
Title: Mesencephalic astrocyte-derived neurotrophic factor protects the heart from ischemic damage and is selectively secreted upon sarco/endoplasmic reticulum calcium depletion.

MANF and C-MANF protect neurons intracellularly as efficiently as Ku70.
Title: Mesencephalic astrocyte-derived neurotrophic factor (MANF) has a unique mechanism to rescue apoptotic neurons.

The following Manf gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Manf cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa12057	NM_001108183.1 Latest version!	Rattus norvegicus mesencephalic astrocyte-derived neurotrophic factor (Manf), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
ORa31580	XM_006243775.2 Latest version!	Rattus norvegicus mesencephalic astrocyte-derived neurotrophic factor (Manf), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Manf ( NM_001108183.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001101653.1 Rattus norvegicus mesencephalic astrocyte-derived neurotrophic factor (Manf), mRNA.

Price & Availability↑

CloneID	ORa12057
Clone ID Related Accession (Same CDS sequence)	NM_001108183.1
Accession Version	NM_001108183.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 369bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Rattus norvegicus(Norway rat)
Product	mesencephalic astrocyte-derived neurotrophic factor precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473954.2. On or before Oct 4, 2007 this sequence version replaced XM_236614.4, XM_001072112.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CB809069.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01906347, SAMN01906352 [ECO:0000348] ##Evidence-Data-END##

ATGTGGGCTA CGCGCGGGCT GGCGGTAGCG CTGGCCCTGA GCGTTCTGCC TGACAGTCGG 
GCGCTACGGC CAGGCGACTG CGAAGTTTGT ATTTCTTATC TGGGGAGATT TTACCAGGAC 
CTCAAAGACA GGGATGTCAC ATTTTCACCA GCCACTATTG AAGAAGAACT TATAAAGTTC 
TGCCGGGAAG CAAGAGGCAA AGAAAATCGG TTGTGCTACT ACATTGGAGC CACAGATGAT 
GCCGCCACCA AGATCATCAA TGAGGTATCG AAGCCTCTGG CCCACCATAT CCCTGTGGAG 
AAGATCTGTG AGAAGCTGAA GAAGAAAGAC AGCCAGATCT GCGAGCTAAA ATACGGTGAG 
TGTGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001101653.1
CDS	66..434
Translation	MWATRGLAVALALSVLPDSRALRPGDCEVCISYLGRFYQDLKDRDVTFSPATIEEELIKFCREARGKENRLCYYIGATDDAATKIINEVSKPLAHHIPVEKICEKLKKKDSQICELKYGECD

Target ORF information:

RefSeq Version	NM_001108183.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus mesencephalic astrocyte-derived neurotrophic factor (Manf), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001108183.1

ORF Insert Sequence:

ATGTGGGCTA CGCGCGGGCT GGCGGTAGCG CTGGCCCTGA GCGTTCTGCC TGACAGTCGG 
GCGCTACGGC CAGGCGACTG CGAAGTTTGT ATTTCTTATC TGGGGAGATT TTACCAGGAC 
CTCAAAGACA GGGATGTCAC ATTTTCACCA GCCACTATTG AAGAAGAACT TATAAAGTTC 
TGCCGGGAAG CAAGAGGCAA AGAAAATCGG TTGTGCTACT ACATTGGAGC CACAGATGAT 
GCCGCCACCA AGATCATCAA TGAGGTATCG AAGCCTCTGG CCCACCATAT CCCTGTGGAG 
AAGATCTGTG AGAAGCTGAA GAAGAAAGAC AGCCAGATCT GCGAGCTAAA ATACGGTGAG 
TGTGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Manf ( XM_006243775.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006243837.1 Rattus norvegicus mesencephalic astrocyte-derived neurotrophic factor (Manf), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa31580
Clone ID Related Accession (Same CDS sequence)	XM_006243775.2
Accession Version	XM_006243775.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 540bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	mesencephalic astrocyte-derived neurotrophic factor isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005107.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006243775.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGCTA CGCGCGGGCT GGCGGTAGCG CTGGCCCTGA GCGTTCTGCC TGACAGTCGG 
GCGCTACGGC CAGGCGACTG CGAAGTTTGT ATTTCTTATC TGGGGAGATT TTACCAGGAC 
CTCAAAGACA GGGATGTCAC ATTTTCACCA GCCACTATTG AAGAAGAACT TATAAAGTTC 
TGCCGGGAAG CAAGAGGCAA AGAAAATCGG TTGTGCTACT ACATTGGAGC CACAGATGAT 
GCCGCCACCA AGATCATCAA TGAGGTATCG AAGCCTCTGG CCCACCATAT CCCTGTGGAG 
AAGATCTGTG AGAAGCTGAA GAAGAAAGAC AGCCAGATCT GCGAGCTAAA ATACGACAAG 
CAGATTGACC TGAGCACAGT GGACCTGAAG AAGCTCCGGG TGAAAGAGCT GAAGAAAATC 
CTGGACGATT GGGGGGAGAT GTGCAAAGGC TGTGCAGAGA AGTCTGACTA TATCCGGAAG 
ATAAATGAAC TCATGCCTAA ATATGCCCCC AAGGCGGCCA GCGCACGGAC TGATCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006243837.1
CDS	61..600
Translation	MWATRGLAVALALSVLPDSRALRPGDCEVCISYLGRFYQDLKDRDVTFSPATIEEELIKFCREARGKENRLCYYIGATDDAATKIINEVSKPLAHHIPVEKICEKLKKKDSQICELKYDKQIDLSTVDLKKLRVKELKKILDDWGEMCKGCAEKSDYIRKINELMPKYAPKAASARTDL

Target ORF information:

RefSeq Version	XM_006243775.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus mesencephalic astrocyte-derived neurotrophic factor (Manf), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006243775.2

ORF Insert Sequence:

ATGTGGGCTA CGCGCGGGCT GGCGGTAGCG CTGGCCCTGA GCGTTCTGCC TGACAGTCGG 
GCGCTACGGC CAGGCGACTG CGAAGTTTGT ATTTCTTATC TGGGGAGATT TTACCAGGAC 
CTCAAAGACA GGGATGTCAC ATTTTCACCA GCCACTATTG AAGAAGAACT TATAAAGTTC 
TGCCGGGAAG CAAGAGGCAA AGAAAATCGG TTGTGCTACT ACATTGGAGC CACAGATGAT 
GCCGCCACCA AGATCATCAA TGAGGTATCG AAGCCTCTGG CCCACCATAT CCCTGTGGAG 
AAGATCTGTG AGAAGCTGAA GAAGAAAGAC AGCCAGATCT GCGAGCTAAA ATACGACAAG 
CAGATTGACC TGAGCACAGT GGACCTGAAG AAGCTCCGGG TGAAAGAGCT GAAGAAAATC 
CTGGACGATT GGGGGGAGAT GTGCAAAGGC TGTGCAGAGA AGTCTGACTA TATCCGGAAG 
ATAAATGAAC TCATGCCTAA ATATGCCCCC AAGGCGGCCA GCGCACGGAC TGATCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Spatiotemporal expression of MANF in the developing rat brain.
PloS one9(2)e90433(2014)
Wang H,Ke Z,Alimov A,Xu M,Frank JA,Fang S,Luo J

Mesencephalic astrocyte-derived neurotrophic factor (MANF) secretion and cell surface binding are modulated by KDEL receptors.
The Journal of biological chemistry288(6)4209-25(2013 Feb)
Henderson MJ,Richie CT,Airavaara M,Wang Y,Harvey BK

Upregulation of mesencephalic astrocyte-derived neurotrophic factor in glial cells is associated with ischemia-induced glial activation.
Journal of neuroinflammation9254(2012 Nov)
Shen Y,Sun A,Wang Y,Cha D,Wang H,Wang F,Feng L,Fang S,Shen Y