Ppp2r5a cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ppp2r5a
Entrez Gene ID	312754
Full Name	protein phosphatase 2, regulatory subunit B', alpha
General protein information	Preferred Names protein phosphatase 2, regulatory subunit B', alpha Names serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit alpha isoform protein phosphatase 2, regulatory subunit B (B56), alpha isoform protein phosphatase 2, regulatory subunit B', alpha isoform
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 13 Map Location: 13q27

mRNA and Protein(s)

mRNA	Protein	Name
NM_001107891.1	NP_001101361.1	serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit alpha isoform

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Ppp2r5a	NP_659129.2
Gallus gallus (chicken)	PPP2R5A	XP_419432.2
Oryza sativa (rice)	Os04g0485100	NP_001053130.1
Pan troglodytes (chimpanzee)	PPP2R5A	XP_001151388.3
Danio rerio (zebrafish)	ppp2r5a	XP_690932.3
Arabidopsis thaliana (thale cress)	ATB' GAMMA	NP_567464.1
Oryza sativa (rice)	Os03g0844500	NP_001051871.1
Oryza sativa (rice)	Os08g0122000	NP_001060884.1
	PPP2R5A	XP_001106979.2
Canis lupus familiaris (dog)	PPP2R5A	XP_005622406.1
Oryza sativa (rice)	Os07g0274800	NP_001059361.1
Xenopus tropicalis (tropical clawed frog)	ppp2r5a	NP_001072157.1
Homo sapiens (human)	PPP2R5A	NP_006234.1
Bos taurus (cattle)	PPP2R5A	NP_001075197.1
Rattus norvegicus (Norway rat)	Ppp2r5a	NP_001101361.1
Arabidopsis thaliana (thale cress)	AT3G21650	NP_188802.1

Gene Ontology
Bibliography

Related articles in PubMed

Bod1 regulates protein phosphatase 2A at mitotic kinetochores.
Porter IM, Schleicher K, Porter M, Swedlow JR
Nature communications42677(2013)

A critical role for the protein phosphatase 2A B'? regulatory subunit in dephosphorylation of sphingosine kinase 1.
Pitman MR, Barr RK, Gliddon BL, Magarey AM, Moretti PA, Pitson SM
The international journal of biochemistry & cell biology43(3)342-7(2011 Mar)

miR-1 overexpression enhances Ca(2+) release and promotes cardiac arrhythmogenesis by targeting PP2A regulatory subunit B56alpha and causing CaMKII-dependent hyperphosphorylation of RyR2.
Terentyev D, Belevych AE, Terentyeva R, Martin MM, Malana GE, Kuhn DE, Abdellatif M, Feldman DS, Elton TS, Gy?rke S
Circulation research104(4)514-21(2009 Feb)

Obscurin targets ankyrin-B and protein phosphatase 2A to the cardiac M-line.
Cunha SR, Mohler PJ
The Journal of biological chemistry283(46)31968-80(2008 Nov)

Mitochondrial protein phosphatase 2A regulates cell death induced by simulated ischemia in kidney NRK-52E cells.
Tsao CC, Nica AF, Kurinna SM, Jiffar T, Mumby M, Ruvolo PP
Cell cycle (Georgetown, Tex.)6(19)2377-85(2007 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These findings suggest that activation of a PP2A isoform that contains the B56 alpha regulatory subunit is required for ischemia-induced cell death in kidney epithelial proximal tubule cells.
Title: Mitochondrial protein phosphatase 2A regulates cell death induced by simulated ischemia in kidney NRK-52E cells.

The following Ppp2r5a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ppp2r5a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa06229	NM_001107891.1 Latest version!	Rattus norvegicus protein phosphatase 2, regulatory subunit B', alpha (Ppp2r5a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ppp2r5a ( NM_001107891.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001101361.1 Rattus norvegicus protein phosphatase 2, regulatory subunit B', alpha (Ppp2r5a), mRNA.

Price & Availability↑

CloneID	ORa06229
Clone ID Related Accession (Same CDS sequence)	NM_001107891.1
Accession Version	NM_001107891.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1461bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-27
Organism	Rattus norvegicus(Norway rat)
Product	serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit alpha isoform
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473985.1. On or before Oct 4, 2007 this sequence version replaced XM_232413.4, XM_001066948.1. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGTCGTCGC CGTCGCCGCC CGCACCAGTT GCCTGCGCCG CCATCTCGGC CTCGGAGAAA 
GTGGACGGCT TCACCCGGAA ATCGGTGCGC AAGGCGCAGA GGCAGAAGCG CTCTCAGGGC 
TCGTCGCAGT TCCGCAGCCA GGGCAGCCAG GCGGAGCTGC ACCCCCTGCC CCAGCTCAAA 
GATGCTACAT CAAATGAACA GCAAGAGCTT TTCTGCCAGA AGCTACAACA GTGCTGTGTA 
CTGTTTGATT TCATGGACTC TGTTTCAGAC TTGAAGAGCA AAGAAATTAA AAGAGCAACG 
CTCAATGAGC TGGTTGAGTA TGTTTCGACT AACCGTGGTG TAATTGTTGA ATCAGCGTAT 
TCTGATATTG TAAAAATGAT CAGTGCTAAC ATCTTCCGGA CACTTCCTCC AAGTGATAAC 
CCAGACTTTG ACCCGGAAGA GGATGAGCCC ACACTTGAGG CCTCTTGGCC TCACATACAG 
CTGGTGTATG AATTCTTCTT GAGATTCTTG GAGAGTCCTG ATTTCCAGCC CAGCATTGCA 
AAGCGATACA TTGACCAGAA GTTTGTACAA CAGCTCCTGG AGCTTTTCGA CAGTGAAGAC 
CCACGAGAGC GTGACTTCCT GAAGACCGTC CTGCACCGGA TTTACGGGAA GTTCCTGGGA 
ATGAGGGCGT TCATCAGAAA GCAAACCAAC AACATCTTCC TCAGGTTTAT CTATGAAACG 
GAGCATTTCA ATGGAGTGGC TGAGCTCCTT GAAATACTGG GAAGTATCAT CAATGGCTTT 
GCGTTGCCAC TGAAAGCAGA ACATAAGCAG TTTCTAATGA AGGTTCTTAT TCCTATGCAT 
ACTGCAAAAG GACTGGCTCT GTTTCATGCT CAGCTGGCCT ACTGTGTTGT GCAGTTCCTG 
GAGAAAGACA CGACACTGAC AGAGCCGGTG ATCAGAGGAC TCCTGAAATT TTGGCCAAAA 
ACATGCAGTC AGAAAGAGGT GATGTTTTTA GGAGAAATTG AAGAGATCTT AGACGTCATT 
GAGCCAACAC AATTCAAAAA GATTGAAGAG CCGCTTTTTA AACAGATATC CAAGTGTGTC 
TCCAGTTCTC ACTTCCAGGT TGCAGAGAGA GCACTGTACT TCTGGAATAA CGAATACATT 
CTCAGTTTGA TTGAGGAGAA CATCGATAAG ATCCTGCCCA TCATGTTTGC CAGTTTGTAT 
AAAATTTCCA AAGAACACTG GAATCAGACT ATTGTAGCTC TGGTATACAA CGTGCTGAAA 
ACCCTAATGG AGATGAACGG GAAGCTTTTT GATGACCTTA CTAGTTCCTA CAAGGCTGAA 
AGACAGAGAG AGAAGAAGAA AGAACTGGAA CGCGAAGAAT TATGGAAAAA GTTAGAAGAG 
TTGAAGCTGA AGAAGGCTCT AGAGAAACAG AACAATGCTT ACAACATGCA CAGTATTCTC 
AGCAATACCA GTGCCCAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001101361.1
CDS	178..1638
Translation	MSSPSPPAPVACAAISASEKVDGFTRKSVRKAQRQKRSQGSSQFRSQGSQAELHPLPQLKDATSNEQQELFCQKLQQCCVLFDFMDSVSDLKSKEIKRATLNELVEYVSTNRGVIVESAYSDIVKMISANIFRTLPPSDNPDFDPEEDEPTLEASWPHIQLVYEFFLRFLESPDFQPSIAKRYIDQKFVQQLLELFDSEDPRERDFLKTVLHRIYGKFLGMRAFIRKQTNNIFLRFIYETEHFNGVAELLEILGSIINGFALPLKAEHKQFLMKVLIPMHTAKGLALFHAQLAYCVVQFLEKDTTLTEPVIRGLLKFWPKTCSQKEVMFLGEIEEILDVIEPTQFKKIEEPLFKQISKCVSSSHFQVAERALYFWNNEYILSLIEENIDKILPIMFASLYKISKEHWNQTIVALVYNVLKTLMEMNGKLFDDLTSSYKAERQREKKKELEREELWKKLEELKLKKALEKQNNAYNMHSILSNTSAQ

Target ORF information:

RefSeq Version	NM_001107891.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus protein phosphatase 2, regulatory subunit B', alpha (Ppp2r5a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001107891.1

ORF Insert Sequence:

ATGTCGTCGC CGTCGCCGCC CGCACCAGTT GCCTGCGCCG CCATCTCGGC CTCGGAGAAA 
GTGGACGGCT TCACCCGGAA ATCGGTGCGC AAGGCGCAGA GGCAGAAGCG CTCTCAGGGC 
TCGTCGCAGT TCCGCAGCCA GGGCAGCCAG GCGGAGCTGC ACCCCCTGCC CCAGCTCAAA 
GATGCTACAT CAAATGAACA GCAAGAGCTT TTCTGCCAGA AGCTACAACA GTGCTGTGTA 
CTGTTTGATT TCATGGACTC TGTTTCAGAC TTGAAGAGCA AAGAAATTAA AAGAGCAACG 
CTCAATGAGC TGGTTGAGTA TGTTTCGACT AACCGTGGTG TAATTGTTGA ATCAGCGTAT 
TCTGATATTG TAAAAATGAT CAGTGCTAAC ATCTTCCGGA CACTTCCTCC AAGTGATAAC 
CCAGACTTTG ACCCGGAAGA GGATGAGCCC ACACTTGAGG CCTCTTGGCC TCACATACAG 
CTGGTGTATG AATTCTTCTT GAGATTCTTG GAGAGTCCTG ATTTCCAGCC CAGCATTGCA 
AAGCGATACA TTGACCAGAA GTTTGTACAA CAGCTCCTGG AGCTTTTCGA CAGTGAAGAC 
CCACGAGAGC GTGACTTCCT GAAGACCGTC CTGCACCGGA TTTACGGGAA GTTCCTGGGA 
ATGAGGGCGT TCATCAGAAA GCAAACCAAC AACATCTTCC TCAGGTTTAT CTATGAAACG 
GAGCATTTCA ATGGAGTGGC TGAGCTCCTT GAAATACTGG GAAGTATCAT CAATGGCTTT 
GCGTTGCCAC TGAAAGCAGA ACATAAGCAG TTTCTAATGA AGGTTCTTAT TCCTATGCAT 
ACTGCAAAAG GACTGGCTCT GTTTCATGCT CAGCTGGCCT ACTGTGTTGT GCAGTTCCTG 
GAGAAAGACA CGACACTGAC AGAGCCGGTG ATCAGAGGAC TCCTGAAATT TTGGCCAAAA 
ACATGCAGTC AGAAAGAGGT GATGTTTTTA GGAGAAATTG AAGAGATCTT AGACGTCATT 
GAGCCAACAC AATTCAAAAA GATTGAAGAG CCGCTTTTTA AACAGATATC CAAGTGTGTC 
TCCAGTTCTC ACTTCCAGGT TGCAGAGAGA GCACTGTACT TCTGGAATAA CGAATACATT 
CTCAGTTTGA TTGAGGAGAA CATCGATAAG ATCCTGCCCA TCATGTTTGC CAGTTTGTAT 
AAAATTTCCA AAGAACACTG GAATCAGACT ATTGTAGCTC TGGTATACAA CGTGCTGAAA 
ACCCTAATGG AGATGAACGG GAAGCTTTTT GATGACCTTA CTAGTTCCTA CAAGGCTGAA 
AGACAGAGAG AGAAGAAGAA AGAACTGGAA CGCGAAGAAT TATGGAAAAA GTTAGAAGAG 
TTGAAGCTGA AGAAGGCTCT AGAGAAACAG AACAATGCTT ACAACATGCA CAGTATTCTC 
AGCAATACCA GTGCCCAGTA A