Nelfb cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nelfb
Entrez Gene ID	311796
Full Name	negative elongation factor complex member B
Synonyms	Cobra1,RGD1307832
General protein information	Preferred Names negative elongation factor complex member B Names negative elongation factor B cofactor of BRCA1 negative elongation factor protein B
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 3 Map Location: 3p13

mRNA and Protein(s)

mRNA	Protein	Name
NM_001107817.3	NP_001101287.3	negative elongation factor B

Pathways from BioSystems

Homologies

	COBRA1	XP_001087434.1
Rattus norvegicus (Norway rat)	Nelfb	NP_001101287.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012109	XP_320419.3
Homo sapiens (human)	NELFB	NP_056271.2
Bos taurus (cattle)	NELFB	XP_002691742.3
Xenopus tropicalis (tropical clawed frog)	nelfb	NP_001011448.1
Mus musculus (house mouse)	Nelfb	NP_067368.2
Gallus gallus (chicken)	COBRA1	NP_001006202.1
Pan troglodytes (chimpanzee)	COBRA1	XP_001136264.3
Canis lupus familiaris (dog)	NELFB	XP_548351.2
Danio rerio (zebrafish)	nelfb	NP_956462.1
Drosophila melanogaster (fruit fly)	NELF-B	NP_572402.1

Gene Ontology
Bibliography

Related articles in PubMed

Pausing of RNA polymerase II regulates mammalian developmental potential through control of signaling networks.
Williams LH, Fromm G, Gokey NG, Henriques T, Muse GW, Burkholder A, Fargo DC, Hu G, Adelman K
Molecular cell58(2)311-322(2015 Apr)

Translational Initiation at a Non-AUG Start Codon for Human and Mouse Negative Elongation Factor-B.
Pan H, Zhao X, Zhang X, Abouelsoud M, Sun J, April C, Amleh A, Fan JB, Hu Y, Li R
PloS one10(5)e0127422(2015)

Negative elongation factor controls energy homeostasis in cardiomyocytes.
Pan H, Qin K, Guo Z, Ma Y, April C, Gao X, Andrews TG, Bokov A, Zhang J, Chen Y, Weintraub ST, Fan JB, Wang D, Hu Y, Aune GJ, Lindsey ML, Li R
Cell reports7(1)79-85(2014 Apr)

A conserved protein motif is required for full modulatory activity of negative elongation factor subunits NELF-A and NELF-B in modifying glucocorticoid receptor-regulated gene induction properties.
Luo M, Lu X, Zhu R, Zhang Z, Chow CC, Li R, Simons SS Jr
The Journal of biological chemistry288(47)34055-72(2013 Nov)

Control of transcriptional elongation.
Kwak H, Lis JT
Annual review of genetics47483-508(2013)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These studies allow us to position the actions of two new modulators of GR-regulated transactivation, NELF-A and NELF-B, relative to other factors in the overall gene induction sequence.
Title: A conserved protein motif is required for full modulatory activity of negative elongation factor subunits NELF-A and NELF-B in modifying glucocorticoid receptor-regulated gene induction properties.

The following Nelfb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nelfb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa47068	NM_001107817.3 Latest version!	Rattus norvegicus negative elongation factor complex member B (Nelfb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nelfb ( NM_001107817.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001101287.3 Rattus norvegicus negative elongation factor complex member B (Nelfb), mRNA.

Price & Availability↑

CloneID	ORa47068
Clone ID Related Accession (Same CDS sequence)	NM_001107817.3
Accession Version	NM_001107817.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1896bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-18
Organism	Rattus norvegicus(Norway rat)
Product	negative elongation factor B
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07051240.1 and BC160853.1. On Jul 3, 2015 this sequence version replaced NM_001107817.2. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## non-AUG initiation codon :: inferred from conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

CTGGCCACAC TGGAGGCGGC GGGCGAACGG GGCTTGGGTG GTCCTCGTGG CACCGTGGAA 
AGGACGTCTG GGGCTCCGTC AGCGTCCGCT ACGGCTCCAG CAGAGCGCGG CGGGGATGGG 
GTACACAGCC GAGCGGCCGT CGGAGCCTCG GCCATGTTTG CGGGGCTCCA GGACCTGGGC 
GTGGCCAATG GCGAGGACCT GAAAGAGACC CTAACCAACT GCACCGAGCC TCTCAAGGCC 
ATTGAGCAAT TTCAGACAGA GAATGGCGTG TTGCTGCCTT CCCTGCAGTC GGCCCTGCCC 
TTCTTGGATC TACACGGGAC ACCGCGGCTG GAGTTCCATC AGTCAGTGTT TGATGAGCTA 
CGCGACAAAC TGCTGGAACG AGTGTCAGCT ATTGCCTCTG AGGGAAAGGC AGAGGAGAGG 
TACAAGAAGC TGGAAGACCT TCTGGAAAAG AGCTTTTCTC TGGTCAAGAT GCCATCCCTA 
CAGCCAGTGG TGATGTGTGT TATGAAACAC TTGCCCAAGG TTCCTGAGAA GAAGCTGAAG 
CTGGTGATGG CTGACAAGGA GTTGTACCGG GCATGTGCTG TGGAAGTGAA GAGGCAGATC 
TGGCAAGATA ACCAGGCTCT CTTTGGTGAT GAAGTCTCAC CACTGTTGAA GCAGTATATC 
CTAGAGAAGG AGAGTGCACT GTTCAGCACG GAGCTCTCTG TCCTGCACAA CTTCTTCAGC 
CCTTCCCCCA AGACCAGGCG TCAGGGAGAG GTGGTGCAGA AGCTGACACA GATGGTAGGG 
AAGAACGTGA AGCTGTATGA CATGGTGCTG CAGTTCTTGC GCACACTGTT CCTTCGGACC 
AGGAATGTGC ACTACTGCAC GCTACGAGCC GAGCTGCTGA TGTCTTTGCA TGACCTGGAT 
GTCAGTGATA TCTGCACCGT GGATCCCTGC CATAAGTTCA CCTGGTGCCT GGATGCCTGC 
ATCCGTGAGC GGTTTGTGGA CAGCAAACGA GCCCGAGAGC TGCAGGGATT CCTTGATGGT 
GTGAAGAAGG GCCAGGAACA AGTTTTGGGG GACTTGTCTA TGATCCTGTG TGACCCTTTT 
GCCATCAACA CTCTGTCCCT GAGCACCATC AGGCACTTGC AGGAGCTGGT TAGCCAGGAG 
ACCCTGCCCA GGGACAGTCC CGACCTCCTC CTCCTCCTTA GGCTGCTGGC ACTGGGCCAA 
GGTGCATGGG ACTTAATAGA CAGCCAGGTC TTCAAGGAGC CCAAGATGGA GGCAGAGCTC 
ATCACCAAGT TCCTGCCGAT GCTCATGTCC TTCGTGGTGG ATGACTACAC TTTCAACGTA 
GATCAGAAGC TTCCAGCTGA GGAGAAAGCC CCAGTCACAT ATCCCAACAC GCTTCCTGAA 
AGCTTCACCA AGTTTCTGCA GGAGCAGCGC ATGGCTTGTG AGGTGGGACT GTACTATGTT 
CTACACATCA CCAAGCAGAG AAACAAGAAT GCTCTCCTGC GCCTGCTGCC TGGTCTTGTG 
GAAACCTTTG GTGACCTGGC CTTCAGTGAC ATCTTCCTCC ACCTACTCAC TGGGAGTCTG 
GCGCTGTTGG CTGATGAATT TGCCCTGGAG GACTTTTGCA GCAGCCTCTT TGATGGCTTC 
TTCCTCACTG CCTCTCCCAG GAAGGACAAT GTACACCGCC ATGTGCTGAG GCTTCTCCTT 
CACTTGCATG CGAGAGTGGC CCCTTCCAAG CTGGAGGCGT TGCAGAAGGC GCTGGAGCCC 
ACGGGCCAGA GCGGGGAGGC TGTGAAGGAG CTCTACTCCC AGCTTGGTGA GAAGTTGGAG 
CAGTTGGACC ATCGGAAGCC CAGTCCTCCC CAAGCTGCAG AGACCCCCGC ACTGGAGCTG 
CCTCTTCCCA GTGTGCCTGC TCCAGCCGCA CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001101287.3
CDS	67..1962
Translation	LATLEAAGERGLGGPRGTVERTSGAPSASATAPAERGGDGVHSRAAVGASAMFAGLQDLGVANGEDLKETLTNCTEPLKAIEQFQTENGVLLPSLQSALPFLDLHGTPRLEFHQSVFDELRDKLLERVSAIASEGKAEERYKKLEDLLEKSFSLVKMPSLQPVVMCVMKHLPKVPEKKLKLVMADKELYRACAVEVKRQIWQDNQALFGDEVSPLLKQYILEKESALFSTELSVLHNFFSPSPKTRRQGEVVQKLTQMVGKNVKLYDMVLQFLRTLFLRTRNVHYCTLRAELLMSLHDLDVSDICTVDPCHKFTWCLDACIRERFVDSKRARELQGFLDGVKKGQEQVLGDLSMILCDPFAINTLSLSTIRHLQELVSQETLPRDSPDLLLLLRLLALGQGAWDLIDSQVFKEPKMEAELITKFLPMLMSFVVDDYTFNVDQKLPAEEKAPVTYPNTLPESFTKFLQEQRMACEVGLYYVLHITKQRNKNALLRLLPGLVETFGDLAFSDIFLHLLTGSLALLADEFALEDFCSSLFDGFFLTASPRKDNVHRHVLRLLLHLHARVAPSKLEALQKALEPTGQSGEAVKELYSQLGEKLEQLDHRKPSPPQAAETPALELPLPSVPAPAAL

Target ORF information:

RefSeq Version	NM_001107817.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus negative elongation factor complex member B (Nelfb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001107817.3

ORF Insert Sequence:

CTGGCCACAC TGGAGGCGGC GGGCGAACGG GGCTTGGGTG GTCCTCGTGG CACCGTGGAA 
AGGACGTCTG GGGCTCCGTC AGCGTCCGCT ACGGCTCCAG CAGAGCGCGG CGGGGATGGG 
GTACACAGCC GAGCGGCCGT CGGAGCCTCG GCCATGTTTG CGGGGCTCCA GGACCTGGGC 
GTGGCCAATG GCGAGGACCT GAAAGAGACC CTAACCAACT GCACCGAGCC TCTCAAGGCC 
ATTGAGCAAT TTCAGACAGA GAATGGCGTG TTGCTGCCTT CCCTGCAGTC GGCCCTGCCC 
TTCTTGGATC TACACGGGAC ACCGCGGCTG GAGTTCCATC AGTCAGTGTT TGATGAGCTA 
CGCGACAAAC TGCTGGAACG AGTGTCAGCT ATTGCCTCTG AGGGAAAGGC AGAGGAGAGG 
TACAAGAAGC TGGAAGACCT TCTGGAAAAG AGCTTTTCTC TGGTCAAGAT GCCATCCCTA 
CAGCCAGTGG TGATGTGTGT TATGAAACAC TTGCCCAAGG TTCCTGAGAA GAAGCTGAAG 
CTGGTGATGG CTGACAAGGA GTTGTACCGG GCATGTGCTG TGGAAGTGAA GAGGCAGATC 
TGGCAAGATA ACCAGGCTCT CTTTGGTGAT GAAGTCTCAC CACTGTTGAA GCAGTATATC 
CTAGAGAAGG AGAGTGCACT GTTCAGCACG GAGCTCTCTG TCCTGCACAA CTTCTTCAGC 
CCTTCCCCCA AGACCAGGCG TCAGGGAGAG GTGGTGCAGA AGCTGACACA GATGGTAGGG 
AAGAACGTGA AGCTGTATGA CATGGTGCTG CAGTTCTTGC GCACACTGTT CCTTCGGACC 
AGGAATGTGC ACTACTGCAC GCTACGAGCC GAGCTGCTGA TGTCTTTGCA TGACCTGGAT 
GTCAGTGATA TCTGCACCGT GGATCCCTGC CATAAGTTCA CCTGGTGCCT GGATGCCTGC 
ATCCGTGAGC GGTTTGTGGA CAGCAAACGA GCCCGAGAGC TGCAGGGATT CCTTGATGGT 
GTGAAGAAGG GCCAGGAACA AGTTTTGGGG GACTTGTCTA TGATCCTGTG TGACCCTTTT 
GCCATCAACA CTCTGTCCCT GAGCACCATC AGGCACTTGC AGGAGCTGGT TAGCCAGGAG 
ACCCTGCCCA GGGACAGTCC CGACCTCCTC CTCCTCCTTA GGCTGCTGGC ACTGGGCCAA 
GGTGCATGGG ACTTAATAGA CAGCCAGGTC TTCAAGGAGC CCAAGATGGA GGCAGAGCTC 
ATCACCAAGT TCCTGCCGAT GCTCATGTCC TTCGTGGTGG ATGACTACAC TTTCAACGTA 
GATCAGAAGC TTCCAGCTGA GGAGAAAGCC CCAGTCACAT ATCCCAACAC GCTTCCTGAA 
AGCTTCACCA AGTTTCTGCA GGAGCAGCGC ATGGCTTGTG AGGTGGGACT GTACTATGTT 
CTACACATCA CCAAGCAGAG AAACAAGAAT GCTCTCCTGC GCCTGCTGCC TGGTCTTGTG 
GAAACCTTTG GTGACCTGGC CTTCAGTGAC ATCTTCCTCC ACCTACTCAC TGGGAGTCTG 
GCGCTGTTGG CTGATGAATT TGCCCTGGAG GACTTTTGCA GCAGCCTCTT TGATGGCTTC 
TTCCTCACTG CCTCTCCCAG GAAGGACAAT GTACACCGCC ATGTGCTGAG GCTTCTCCTT 
CACTTGCATG CGAGAGTGGC CCCTTCCAAG CTGGAGGCGT TGCAGAAGGC GCTGGAGCCC 
ACGGGCCAGA GCGGGGAGGC TGTGAAGGAG CTCTACTCCC AGCTTGGTGA GAAGTTGGAG 
CAGTTGGACC ATCGGAAGCC CAGTCCTCCC CAAGCTGCAG AGACCCCCGC ACTGGAGCTG 
CCTCTTCCCA GTGTGCCTGC TCCAGCCGCA CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Pausing of RNA polymerase II regulates mammalian developmental potential through control of signaling networks.
Molecular cell58(2)311-322(2015 Apr)
Williams LH,Fromm G,Gokey NG,Henriques T,Muse GW,Burkholder A,Fargo DC,Hu G,Adelman K

Translational Initiation at a Non-AUG Start Codon for Human and Mouse Negative Elongation Factor-B.
PloS one10(5)e0127422(2015)
Pan H,Zhao X,Zhang X,Abouelsoud M,Sun J,April C,Amleh A,Fan JB,Hu Y,Li R

Negative elongation factor controls energy homeostasis in cardiomyocytes.
Cell reports7(1)79-85(2014 Apr)
Pan H,Qin K,Guo Z,Ma Y,April C,Gao X,Andrews TG,Bokov A,Zhang J,Chen Y,Weintraub ST,Fan JB,Wang D,Hu Y,Aune GJ,Lindsey ML,Li R

A conserved protein motif is required for full modulatory activity of negative elongation factor subunits NELF-A and NELF-B in modifying glucocorticoid receptor-regulated gene induction properties.
The Journal of biological chemistry288(47)34055-72(2013 Nov)
Luo M,Lu X,Zhu R,Zhang Z,Chow CC,Li R,Simons SS Jr

Control of transcriptional elongation.
Annual review of genetics47483-508(2013)
Kwak H,Lis JT