Manba cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Manba
Entrez Gene ID	310864
Full Name	mannosidase beta
General protein information	Preferred Names mannosidase beta Names beta-mannosidase lysosomal beta A mannosidase mannanase mannase mannosidase beta A mannosidase, beta A, lysosomal
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 2 Map Location: 2q43

mRNA and Protein(s)

mRNA	Protein	Name
NM_001031655.1	NP_001026825.1	beta-mannosidase precursor

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	manba	NP_956453.1
Magnaporthe oryzae (rice blast fungus)	MGG_01178	XP_003714062.1
Neurospora crassa	NCU00890	XP_964806.1
Pan troglodytes (chimpanzee)	MANBA	XP_001169005.1
	MANBA	XP_001101549.2
Drosophila melanogaster (fruit fly)	CG12582	NP_649436.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000143	XP_311001.5
Xenopus tropicalis (tropical clawed frog)	manba	XP_002934804.2
Homo sapiens (human)	MANBA	NP_005899.3
Bos taurus (cattle)	MANBA	NP_776812.1
Gallus gallus (chicken)	MANBA	XP_420666.3
Danio rerio (zebrafish)	LOC100331766	XP_002664183.1
Caenorhabditis elegans (roundworm)	C33G3.4	NP_510342.1
Canis lupus familiaris (dog)	MANBA	XP_005639293.1
Mus musculus (house mouse)	Manba	NP_081564.3
Rattus norvegicus (Norway rat)	Manba	NP_001026825.1

Gene Ontology
Bibliography

Related articles in PubMed

Mice lacking mannose 6-phosphate uncovering enzyme activity have a milder phenotype than mice deficient for N-acetylglucosamine-1-phosphotransferase activity.
Boonen M, Vogel P, Platt KA, Dahms N, Kornfeld S
Molecular biology of the cell20(20)4381-9(2009 Oct)

Beta-mannosidosis mice: a model for the human lysosomal storage disease.
Zhu M, Lovell KL, Patterson JS, Saunders TL, Hughes ED, Friderici KH
Human molecular genetics15(3)493-500(2006 Feb)

Rat brain contains high levels of mannose-6-phosphorylated glycoproteins including lysosomal enzymes and palmitoyl-protein thioesterase, an enzyme implicated in infantile neuronal lipofuscinosis.
Sleat DE, Sohar I, Lackland H, Majercak J, Lobel P
The Journal of biological chemistry271(32)19191-8(1996 Aug)

A gene (Bmn) controlling beta-mannosidase activity in the mouse is located in the distal part of chromosome 3.
Lundin LG
Biochemical genetics25(7-8)603-10(1987 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Manba gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Manba cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa42820	NM_001031655.1 Latest version!	Rattus norvegicus mannosidase beta (Manba), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Manba ( NM_001031655.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001026825.1 Rattus norvegicus mannosidase beta (Manba), mRNA.

Price & Availability↑

CloneID	ORa42820
Clone ID Related Accession (Same CDS sequence)	NM_001031655.1
Accession Version	NM_001031655.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2646bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Rattus norvegicus(Norway rat)
Product	beta-mannosidase precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC099094.1. On Aug 12, 2005 this sequence version replaced XM_227701.3. ##Evidence-Data-START## Transcript exon combination :: BC099094.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGCATCTCC ACCTGCTCTT TCTGTTGGCG CTGTGCGGGG CAGGCTGTAT GGCCGCAGGT 
CCCAGCTACA GCCTGAGAGG GAGCTGGCGG GTGAGCAACG GGAATAGTTC CCTGGAGCTG 
CCGGCTACTG TCCCTGGCTA TGTGCACAGC GCCCTGCAGC AGCACGGGCT GATCCAGGAT 
CCTTACTACA GATTTAATGA CCTGAACTAC AGATGGATTT CCTTAGATAA CTGGACCTAC 
AGCACAGAAT TTAAAATCCC CTTTAATCGC AGTGAATGGC AGAAGGTAAA GTTGATTTTT 
GACGGAGTCG ATACAGTTGC AGAAATCCTG TTCAATAATG TCACTATTGG GAAGACAGAC 
AACATGTTCA CCAGATATAG CTTTGACGTC ACCAATGTGG TCAAAGACGT GAACTCCCTA 
AAGCTGCGGT TCCAGTCAGC AGTGCAGTAT GCTGAGTGTC AGAGCAAAGC TCACACTCAA 
TACCGGGTGC CCCCAGAGTG CCCCCCAGTG GAACAGAAGG GTGAATGCCA CGTCAACTTC 
ATCCGAAAGG AGCAGTGCTC CTTCAGCTGG GACTGGGGGC CTTCCTTTCC CTCTCAAGGA 
ATCTGGAAAG ACGTTAGAAT CGAAGCCTAT AACATCGCTC ATCTGGATCA CCTGACATTT 
TTGCCATTGT ATGATAACAC TTCTCAGGCG TGGACTATTG AAATAGAGGC TTCTTTTGAT 
GTTGTCAGCA CAAAGCCAGT TGGTGGTCAG GTGACTATAG CCATCCCCGA ACTGAAAACA 
CAGCAGGCAA ACCACATTGA ACTTCAACAT GGGCAAAGGA TTGTTAAACT CCTTGTCAAA 
ATTAGGAAGG ACGTCACCGT GGAGACCTGG TGGCCTCATG GACATGGGAA CCAGACTGGG 
TACAACACGA CAATTCTCTT TGCGCTGGAT GGAGGCTTAA AAATTGAAAA AGCAGCCAAG 
GTTTATTTCA GAACAGTGCA ACTTATAGAA GAACCTATAA CAGGGTCTCC TGGTCTGAGT 
TTCTACTTCA AAATTAATGG GCTTCCTATA TTTCTGAAAG GCTCAAACTG GATCCCAGCT 
GACTCATTCC AAGACAAAGT CACTTCTGAA CTGCTGCAGC TCCTTCTCCA GTCCGCCGTG 
GATGCTAACA TGAACACTCT GCGCGTGTGG GGGGGTGGCA TCTACGAGCA GGATGAATTC 
TATGCACTGT GTGACGAACT TGGAATAATG GTGTGGCAGG ACTTCATGTT TGCCAGTGCC 
CTCTATCCAA CTGAGCCAGG CTTCCTAGAG TCAGTGAGAA AAGAAGTCAC TTACCAGGTC 
AGGAGACTGA AGTCTCACCC CTCCGTCATC ATCTGGAGTG GTAATAATGA GAATGAAGTG 
GCTCTCAGGG TGAACTGGTT CCATGTAAAC CCCAGGGACC TGGGGACTTA CATCAACGAC 
TATGTGACCC TCTACGTGAA AACCATCAGG GAGATTGTCT TATCGGAAGA TAGGAGTCGT 
CCGTTCATTG CATCCAGCCC AACCAATGGG GTCAAAACCA TGACGGAAGG CTGGATCTCT 
AAGGACCCTT ATAGCACCCA GTATGGCGAC ATGCATTTTT ACGACTATTT CAGTGACTGC 
TGGGACTGGA AGGTCTTTCC AAAAGCTCGA TTAGTGTCTG AATATGGCTA CCAGTCCTGG 
CCTTCCTTCA GCACACTACA AAAGGTCTCT CGTCAAGAGG ACTGGTCTTA CAGCAGCCGC 
TTCTCCCTTC ATCGGCAGCA TCATGGAAAT GGCAACAATG AAATGCTTCA CCAGGTACAG 
CTTCATTTCC AACTCCCCCA AAGGAGAGAC CCAGTGCGTG CATTTAAAGA TACCATCTAC 
CTCACTCAGG TGATGCAGGC CCAGTGTATC AAAACAGAAA CCGAGTTCTA CCTGCGCAGC 
CGCAGCGAGA TAGTGAATGG AGAAGGCCAC ACCATGGGGG CACTGTACTG GCAGCTGAAT 
GATATCTGGC AGGCTCCTTC CTGGGCCTCT CTTGAGTACG GAGGGAAATG GAAAATGCTT 
CATTACTTCG CTCAGCGTTT CTTCGCTCCG CTGCTGCCAG TCGGTTTTGA AGATGAAGGC 
GTTTTTTATG TCTACGGTGT GTCAGATCTT CACAAGGACT ACCCCACAAA GCTCACCGTG 
AGGCTCCACC GCTGGAGCTC CCAGAAGCCT TTGTGCACCT TCGTGAGTTT GAGTGCTGTG 
ATTAAGGCAG GGGAGGCGAT GGTTCTGTTC CAGATGCCAG TGTCTAAGCT GCTGAAGAGG 
TGTAAAGAAT GCACAAGGGA CACCTGTGTG GTTTCCTTTT ACCTCTCAAC TGACAATGAA 
CTCTTCAGTC CAACCAACTA CCATTTCCTG TCCTCCCTGA AGGATGCCAA AGGGATGGTC 
AAGGCCAACA TCACTGTCAG CATCTCTCAG AAGGGTGATC TATTTGTTTT TGATCTGAAA 
TCGTCAACAT CAGCCATCAC TCCCTTTGTT TGGTTGGATG TAGGAAGCAT CCCGGGGAGA 
TTTAGTGACA ACGGCTTCCT CATGATTAAG AAGGAGCTCT CGGTACTGTT TTACCCATGG 
AAGCCCACCA GCAAGAGTGA GCTGCAGCAG GCCTTCAGTG TCACCTCCCT GACAGATCTG 
TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001026825.1
CDS	16..2661
Translation	MHLHLLFLLALCGAGCMAAGPSYSLRGSWRVSNGNSSLELPATVPGYVHSALQQHGLIQDPYYRFNDLNYRWISLDNWTYSTEFKIPFNRSEWQKVKLIFDGVDTVAEILFNNVTIGKTDNMFTRYSFDVTNVVKDVNSLKLRFQSAVQYAECQSKAHTQYRVPPECPPVEQKGECHVNFIRKEQCSFSWDWGPSFPSQGIWKDVRIEAYNIAHLDHLTFLPLYDNTSQAWTIEIEASFDVVSTKPVGGQVTIAIPELKTQQANHIELQHGQRIVKLLVKIRKDVTVETWWPHGHGNQTGYNTTILFALDGGLKIEKAAKVYFRTVQLIEEPITGSPGLSFYFKINGLPIFLKGSNWIPADSFQDKVTSELLQLLLQSAVDANMNTLRVWGGGIYEQDEFYALCDELGIMVWQDFMFASALYPTEPGFLESVRKEVTYQVRRLKSHPSVIIWSGNNENEVALRVNWFHVNPRDLGTYINDYVTLYVKTIREIVLSEDRSRPFIASSPTNGVKTMTEGWISKDPYSTQYGDMHFYDYFSDCWDWKVFPKARLVSEYGYQSWPSFSTLQKVSRQEDWSYSSRFSLHRQHHGNGNNEMLHQVQLHFQLPQRRDPVRAFKDTIYLTQVMQAQCIKTETEFYLRSRSEIVNGEGHTMGALYWQLNDIWQAPSWASLEYGGKWKMLHYFAQRFFAPLLPVGFEDEGVFYVYGVSDLHKDYPTKLTVRLHRWSSQKPLCTFVSLSAVIKAGEAMVLFQMPVSKLLKRCKECTRDTCVVSFYLSTDNELFSPTNYHFLSSLKDAKGMVKANITVSISQKGDLFVFDLKSSTSAITPFVWLDVGSIPGRFSDNGFLMIKKELSVLFYPWKPTSKSELQQAFSVTSLTDLY

Target ORF information:

RefSeq Version	NM_001031655.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus mannosidase beta (Manba), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001031655.1

ORF Insert Sequence:

ATGCATCTCC ACCTGCTCTT TCTGTTGGCG CTGTGCGGGG CAGGCTGTAT GGCCGCAGGT 
CCCAGCTACA GCCTGAGAGG GAGCTGGCGG GTGAGCAACG GGAATAGTTC CCTGGAGCTG 
CCGGCTACTG TCCCTGGCTA TGTGCACAGC GCCCTGCAGC AGCACGGGCT GATCCAGGAT 
CCTTACTACA GATTTAATGA CCTGAACTAC AGATGGATTT CCTTAGATAA CTGGACCTAC 
AGCACAGAAT TTAAAATCCC CTTTAATCGC AGTGAATGGC AGAAGGTAAA GTTGATTTTT 
GACGGAGTCG ATACAGTTGC AGAAATCCTG TTCAATAATG TCACTATTGG GAAGACAGAC 
AACATGTTCA CCAGATATAG CTTTGACGTC ACCAATGTGG TCAAAGACGT GAACTCCCTA 
AAGCTGCGGT TCCAGTCAGC AGTGCAGTAT GCTGAGTGTC AGAGCAAAGC TCACACTCAA 
TACCGGGTGC CCCCAGAGTG CCCCCCAGTG GAACAGAAGG GTGAATGCCA CGTCAACTTC 
ATCCGAAAGG AGCAGTGCTC CTTCAGCTGG GACTGGGGGC CTTCCTTTCC CTCTCAAGGA 
ATCTGGAAAG ACGTTAGAAT CGAAGCCTAT AACATCGCTC ATCTGGATCA CCTGACATTT 
TTGCCATTGT ATGATAACAC TTCTCAGGCG TGGACTATTG AAATAGAGGC TTCTTTTGAT 
GTTGTCAGCA CAAAGCCAGT TGGTGGTCAG GTGACTATAG CCATCCCCGA ACTGAAAACA 
CAGCAGGCAA ACCACATTGA ACTTCAACAT GGGCAAAGGA TTGTTAAACT CCTTGTCAAA 
ATTAGGAAGG ACGTCACCGT GGAGACCTGG TGGCCTCATG GACATGGGAA CCAGACTGGG 
TACAACACGA CAATTCTCTT TGCGCTGGAT GGAGGCTTAA AAATTGAAAA AGCAGCCAAG 
GTTTATTTCA GAACAGTGCA ACTTATAGAA GAACCTATAA CAGGGTCTCC TGGTCTGAGT 
TTCTACTTCA AAATTAATGG GCTTCCTATA TTTCTGAAAG GCTCAAACTG GATCCCAGCT 
GACTCATTCC AAGACAAAGT CACTTCTGAA CTGCTGCAGC TCCTTCTCCA GTCCGCCGTG 
GATGCTAACA TGAACACTCT GCGCGTGTGG GGGGGTGGCA TCTACGAGCA GGATGAATTC 
TATGCACTGT GTGACGAACT TGGAATAATG GTGTGGCAGG ACTTCATGTT TGCCAGTGCC 
CTCTATCCAA CTGAGCCAGG CTTCCTAGAG TCAGTGAGAA AAGAAGTCAC TTACCAGGTC 
AGGAGACTGA AGTCTCACCC CTCCGTCATC ATCTGGAGTG GTAATAATGA GAATGAAGTG 
GCTCTCAGGG TGAACTGGTT CCATGTAAAC CCCAGGGACC TGGGGACTTA CATCAACGAC 
TATGTGACCC TCTACGTGAA AACCATCAGG GAGATTGTCT TATCGGAAGA TAGGAGTCGT 
CCGTTCATTG CATCCAGCCC AACCAATGGG GTCAAAACCA TGACGGAAGG CTGGATCTCT 
AAGGACCCTT ATAGCACCCA GTATGGCGAC ATGCATTTTT ACGACTATTT CAGTGACTGC 
TGGGACTGGA AGGTCTTTCC AAAAGCTCGA TTAGTGTCTG AATATGGCTA CCAGTCCTGG 
CCTTCCTTCA GCACACTACA AAAGGTCTCT CGTCAAGAGG ACTGGTCTTA CAGCAGCCGC 
TTCTCCCTTC ATCGGCAGCA TCATGGAAAT GGCAACAATG AAATGCTTCA CCAGGTACAG 
CTTCATTTCC AACTCCCCCA AAGGAGAGAC CCAGTGCGTG CATTTAAAGA TACCATCTAC 
CTCACTCAGG TGATGCAGGC CCAGTGTATC AAAACAGAAA CCGAGTTCTA CCTGCGCAGC 
CGCAGCGAGA TAGTGAATGG AGAAGGCCAC ACCATGGGGG CACTGTACTG GCAGCTGAAT 
GATATCTGGC AGGCTCCTTC CTGGGCCTCT CTTGAGTACG GAGGGAAATG GAAAATGCTT 
CATTACTTCG CTCAGCGTTT CTTCGCTCCG CTGCTGCCAG TCGGTTTTGA AGATGAAGGC 
GTTTTTTATG TCTACGGTGT GTCAGATCTT CACAAGGACT ACCCCACAAA GCTCACCGTG 
AGGCTCCACC GCTGGAGCTC CCAGAAGCCT TTGTGCACCT TCGTGAGTTT GAGTGCTGTG 
ATTAAGGCAG GGGAGGCGAT GGTTCTGTTC CAGATGCCAG TGTCTAAGCT GCTGAAGAGG 
TGTAAAGAAT GCACAAGGGA CACCTGTGTG GTTTCCTTTT ACCTCTCAAC TGACAATGAA 
CTCTTCAGTC CAACCAACTA CCATTTCCTG TCCTCCCTGA AGGATGCCAA AGGGATGGTC 
AAGGCCAACA TCACTGTCAG CATCTCTCAG AAGGGTGATC TATTTGTTTT TGATCTGAAA 
TCGTCAACAT CAGCCATCAC TCCCTTTGTT TGGTTGGATG TAGGAAGCAT CCCGGGGAGA 
TTTAGTGACA ACGGCTTCCT CATGATTAAG AAGGAGCTCT CGGTACTGTT TTACCCATGG 
AAGCCCACCA GCAAGAGTGA GCTGCAGCAG GCCTTCAGTG TCACCTCCCT GACAGATCTG 
TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mice lacking mannose 6-phosphate uncovering enzyme activity have a milder phenotype than mice deficient for N-acetylglucosamine-1-phosphotransferase activity.
Molecular biology of the cell20(20)4381-9(2009 Oct)
Boonen M,Vogel P,Platt KA,Dahms N,Kornfeld S

Beta-mannosidosis mice: a model for the human lysosomal storage disease.
Human molecular genetics15(3)493-500(2006 Feb)
Zhu M,Lovell KL,Patterson JS,Saunders TL,Hughes ED,Friderici KH

Rat brain contains high levels of mannose-6-phosphorylated glycoproteins including lysosomal enzymes and palmitoyl-protein thioesterase, an enzyme implicated in infantile neuronal lipofuscinosis.
The Journal of biological chemistry271(32)19191-8(1996 Aug)
Sleat DE,Sohar I,Lackland H,Majercak J,Lobel P

A gene (Bmn) controlling beta-mannosidase activity in the mouse is located in the distal part of chromosome 3.
Biochemical genetics25(7-8)603-10(1987 Aug)
Lundin LG