Ch25h cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ch25h
Entrez Gene ID	309527
Full Name	cholesterol 25-hydroxylase
General protein information	Preferred Names cholesterol 25-hydroxylase Names cholesterol 25-hydroxylase cholesterol 25-monooxygenase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q53

mRNA and Protein(s)

mRNA	Protein	Name
XM_006231291.3	XP_006231353.1	cholesterol 25-hydroxylase isoform X1
NM_001025415.1	NP_001020586.1	cholesterol 25-hydroxylase

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	CH25H	XP_003639977.1
Rattus norvegicus (Norway rat)	Ch25h	NP_001020586.1
Danio rerio (zebrafish)	ch25h	NP_001008652.1
Xenopus tropicalis (tropical clawed frog)	LOC100494906	XP_002935478.1
	CH25H	XP_001083208.1
Mus musculus (house mouse)	Ch25h	NP_034020.1
Homo sapiens (human)	CH25H	NP_003947.1
Pan troglodytes (chimpanzee)	CH25H	XP_507901.2
Bos taurus (cattle)	CH25H	NP_001068711.1
Gallus gallus (chicken)	CH25H	NP_001264283.1

Gene Ontology
Bibliography

Related articles in PubMed

Oxysterol gradient generation by lymphoid stromal cells guides activated B cell movement during humoral responses.
Yi T, Wang X, Kelly LM, An J, Xu Y, Sailer AW, Gustafsson JA, Russell DW, Cyster JG
Immunity37(3)535-48(2012 Sep)

Testosterone regulates 25-hydroxycholesterol production in testicular macrophages.
Lukyanenko Y, Chen JJ, Hutson JC
Biology of reproduction67(5)1435-8(2002 Nov)

25-hydroxycholesterol is produced by testicular macrophages during the early postnatal period and influences differentiation of Leydig cells in vitro.
Chen JJ, Lukyanenko Y, Hutson JC
Biology of reproduction66(5)1336-41(2002 May)

Production of 25-hydroxycholesterol by testicular macrophages and its effects on Leydig cells.
Lukyanenko YO, Chen JJ, Hutson JC
Biology of reproduction64(3)790-6(2001 Mar)

cDNA cloning of mouse and human cholesterol 25-hydroxylases, polytopic membrane proteins that synthesize a potent oxysterol regulator of lipid metabolism.
Lund EG, Kerr TA, Sakai J, Li WP, Russell DW
The Journal of biological chemistry273(51)34316-27(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Ch25h gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ch25h cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa24041	NM_001025415.1 Latest version!	Rattus norvegicus cholesterol 25-hydroxylase (Ch25h), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
ORa24041	XM_006231291.3 Latest version!	Rattus norvegicus cholesterol 25-hydroxylase (Ch25h), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ch25h ( NM_001025415.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001020586.1 Rattus norvegicus cholesterol 25-hydroxylase (Ch25h), mRNA.

Price & Availability↑

CloneID	ORa24041
Clone ID Related Accession (Same CDS sequence)	XM_006231291.3 , NM_001025415.1
Accession Version	NM_001025415.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 897bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-05
Organism	Rattus norvegicus(Norway rat)
Product	cholesterol 25-hydroxylase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC097964.1. On Jul 15, 2005 this sequence version replaced XM_220063.3. ##Evidence-Data-START## Transcript exon combination :: BC097964.1 [ECO:0000332] ##Evidence-Data-END##

ATGGCCTGCC ACAACGTTTC GGAGCTCCAA GACCTTGGTT GTTCCAACCA GCTGCTCCTG 
CAGCCTCTCT GGGACAGCAT AAGGACGGGA GAGGCGTCCG CGCGCTCACC CTTCTTCCCA 
GTCATCTTTT CTATCTTCAC TTACCTGGGC TTCTGCCTAC CGTTCGTGGT GCTAGATGTC 
CTCTGTCCCT GGGTGCCCAT CTTGAGACGC TACAAGATCC ACCCGGACTT CTCGCCGTCC 
GTAAGGCAGC TTCTGCCCTG CCTGGGGCTG ACACTCTACC AGCACCTGGT GTTCGTGTTC 
CCAGTGACGC TGATGCACTG GGCGCGCAGC CCCGCGCTGC TGCCGCGGGA GGCCCCCGAG 
CTCTCCCAGC TCCTGAGTCA CGTCCTGATC TGCCTGCTGC TCTTCGACAC CGAGATCTTC 
GCGTGGCATC TGCTGCACCA TAAGGTGCCC TGGCTGTACC GCACCTTCCA CAAGGTGCAT 
CACCAGAACT CGTCCTCCTT CGCGTTGGCT ACCCAATACA TGAGCGTCTG GGAGCTGCTT 
TCTCTGACCT TCTTCGATGT GCTGAACGTC GCGATGCTTC AGTGTCACCC ACTCACCATC 
CTCGTCTTTC ACGTGGTTAA CATCTGGCTG TCGGTGGAGG ACCACTCAGG TTACGACTTT 
CCGTGGTCCA CTCACAGACT TGTGCCGTTT GGCTGGTACG GGGGCGTGGC TCACCACGAC 
CTGCATCACT CTCAGTTTAA CTGCAACTTT GCTCCGTACT TCACACACTG GGACAAAATG 
CTGGGGACCC TGCGTTGCGC ACCCCACTCG AAGCGCCTGT GTGCCGGCAG TGAGAGCTGC 
TTGGACTCCG GAGAGCAATG TACTGTACAC TTGAACCAGA AGAAGAAACA GACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001020586.1
CDS	6..902
Translation	MACHNVSELQDLGCSNQLLLQPLWDSIRTGEASARSPFFPVIFSIFTYLGFCLPFVVLDVLCPWVPILRRYKIHPDFSPSVRQLLPCLGLTLYQHLVFVFPVTLMHWARSPALLPREAPELSQLLSHVLICLLLFDTEIFAWHLLHHKVPWLYRTFHKVHHQNSSSFALATQYMSVWELLSLTFFDVLNVAMLQCHPLTILVFHVVNIWLSVEDHSGYDFPWSTHRLVPFGWYGGVAHHDLHHSQFNCNFAPYFTHWDKMLGTLRCAPHSKRLCAGSESCLDSGEQCTVHLNQKKKQT

Target ORF information:

RefSeq Version	NM_001025415.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus cholesterol 25-hydroxylase (Ch25h), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001025415.1

ORF Insert Sequence:

ATGGCCTGCC ACAACGTTTC GGAGCTCCAA GACCTTGGTT GTTCCAACCA GCTGCTCCTG 
CAGCCTCTCT GGGACAGCAT AAGGACGGGA GAGGCGTCCG CGCGCTCACC CTTCTTCCCA 
GTCATCTTTT CTATCTTCAC TTACCTGGGC TTCTGCCTAC CGTTCGTGGT GCTAGATGTC 
CTCTGTCCCT GGGTGCCCAT CTTGAGACGC TACAAGATCC ACCCGGACTT CTCGCCGTCC 
GTAAGGCAGC TTCTGCCCTG CCTGGGGCTG ACACTCTACC AGCACCTGGT GTTCGTGTTC 
CCAGTGACGC TGATGCACTG GGCGCGCAGC CCCGCGCTGC TGCCGCGGGA GGCCCCCGAG 
CTCTCCCAGC TCCTGAGTCA CGTCCTGATC TGCCTGCTGC TCTTCGACAC CGAGATCTTC 
GCGTGGCATC TGCTGCACCA TAAGGTGCCC TGGCTGTACC GCACCTTCCA CAAGGTGCAT 
CACCAGAACT CGTCCTCCTT CGCGTTGGCT ACCCAATACA TGAGCGTCTG GGAGCTGCTT 
TCTCTGACCT TCTTCGATGT GCTGAACGTC GCGATGCTTC AGTGTCACCC ACTCACCATC 
CTCGTCTTTC ACGTGGTTAA CATCTGGCTG TCGGTGGAGG ACCACTCAGG TTACGACTTT 
CCGTGGTCCA CTCACAGACT TGTGCCGTTT GGCTGGTACG GGGGCGTGGC TCACCACGAC 
CTGCATCACT CTCAGTTTAA CTGCAACTTT GCTCCGTACT TCACACACTG GGACAAAATG 
CTGGGGACCC TGCGTTGCGC ACCCCACTCG AAGCGCCTGT GTGCCGGCAG TGAGAGCTGC 
TTGGACTCCG GAGAGCAATG TACTGTACAC TTGAACCAGA AGAAGAAACA GACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ch25h ( XM_006231291.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006231353.1 Rattus norvegicus cholesterol 25-hydroxylase (Ch25h), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa24041
Clone ID Related Accession (Same CDS sequence)	XM_006231291.3 , NM_001025415.1
Accession Version	XM_006231291.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 897bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	cholesterol 25-hydroxylase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006231291.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTGCC ACAACGTTTC GGAGCTCCAA GACCTTGGTT GTTCCAACCA GCTGCTCCTG 
CAGCCTCTCT GGGACAGCAT AAGGACGGGA GAGGCGTCCG CGCGCTCACC CTTCTTCCCA 
GTCATCTTTT CTATCTTCAC TTACCTGGGC TTCTGCCTAC CGTTCGTGGT GCTAGATGTC 
CTCTGTCCCT GGGTGCCCAT CTTGAGACGC TACAAGATCC ACCCGGACTT CTCGCCGTCC 
GTAAGGCAGC TTCTGCCCTG CCTGGGGCTG ACACTCTACC AGCACCTGGT GTTCGTGTTC 
CCAGTGACGC TGATGCACTG GGCGCGCAGC CCCGCGCTGC TGCCGCGGGA GGCCCCCGAG 
CTCTCCCAGC TCCTGAGTCA CGTCCTGATC TGCCTGCTGC TCTTCGACAC CGAGATCTTC 
GCGTGGCATC TGCTGCACCA TAAGGTGCCC TGGCTGTACC GCACCTTCCA CAAGGTGCAT 
CACCAGAACT CGTCCTCCTT CGCGTTGGCT ACCCAATACA TGAGCGTCTG GGAGCTGCTT 
TCTCTGACCT TCTTCGATGT GCTGAACGTC GCGATGCTTC AGTGTCACCC ACTCACCATC 
CTCGTCTTTC ACGTGGTTAA CATCTGGCTG TCGGTGGAGG ACCACTCAGG TTACGACTTT 
CCGTGGTCCA CTCACAGACT TGTGCCGTTT GGCTGGTACG GGGGCGTGGC TCACCACGAC 
CTGCATCACT CTCAGTTTAA CTGCAACTTT GCTCCGTACT TCACACACTG GGACAAAATG 
CTGGGGACCC TGCGTTGCGC ACCCCACTCG AAGCGCCTGT GTGCCGGCAG TGAGAGCTGC 
TTGGACTCCG GAGAGCAATG TACTGTACAC TTGAACCAGA AGAAGAAACA GACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006231353.1
CDS	2602..3498
Translation	MACHNVSELQDLGCSNQLLLQPLWDSIRTGEASARSPFFPVIFSIFTYLGFCLPFVVLDVLCPWVPILRRYKIHPDFSPSVRQLLPCLGLTLYQHLVFVFPVTLMHWARSPALLPREAPELSQLLSHVLICLLLFDTEIFAWHLLHHKVPWLYRTFHKVHHQNSSSFALATQYMSVWELLSLTFFDVLNVAMLQCHPLTILVFHVVNIWLSVEDHSGYDFPWSTHRLVPFGWYGGVAHHDLHHSQFNCNFAPYFTHWDKMLGTLRCAPHSKRLCAGSESCLDSGEQCTVHLNQKKKQT

Target ORF information:

RefSeq Version	XM_006231291.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus cholesterol 25-hydroxylase (Ch25h), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006231291.3

ORF Insert Sequence:

ATGGCCTGCC ACAACGTTTC GGAGCTCCAA GACCTTGGTT GTTCCAACCA GCTGCTCCTG 
CAGCCTCTCT GGGACAGCAT AAGGACGGGA GAGGCGTCCG CGCGCTCACC CTTCTTCCCA 
GTCATCTTTT CTATCTTCAC TTACCTGGGC TTCTGCCTAC CGTTCGTGGT GCTAGATGTC 
CTCTGTCCCT GGGTGCCCAT CTTGAGACGC TACAAGATCC ACCCGGACTT CTCGCCGTCC 
GTAAGGCAGC TTCTGCCCTG CCTGGGGCTG ACACTCTACC AGCACCTGGT GTTCGTGTTC 
CCAGTGACGC TGATGCACTG GGCGCGCAGC CCCGCGCTGC TGCCGCGGGA GGCCCCCGAG 
CTCTCCCAGC TCCTGAGTCA CGTCCTGATC TGCCTGCTGC TCTTCGACAC CGAGATCTTC 
GCGTGGCATC TGCTGCACCA TAAGGTGCCC TGGCTGTACC GCACCTTCCA CAAGGTGCAT 
CACCAGAACT CGTCCTCCTT CGCGTTGGCT ACCCAATACA TGAGCGTCTG GGAGCTGCTT 
TCTCTGACCT TCTTCGATGT GCTGAACGTC GCGATGCTTC AGTGTCACCC ACTCACCATC 
CTCGTCTTTC ACGTGGTTAA CATCTGGCTG TCGGTGGAGG ACCACTCAGG TTACGACTTT 
CCGTGGTCCA CTCACAGACT TGTGCCGTTT GGCTGGTACG GGGGCGTGGC TCACCACGAC 
CTGCATCACT CTCAGTTTAA CTGCAACTTT GCTCCGTACT TCACACACTG GGACAAAATG 
CTGGGGACCC TGCGTTGCGC ACCCCACTCG AAGCGCCTGT GTGCCGGCAG TGAGAGCTGC 
TTGGACTCCG GAGAGCAATG TACTGTACAC TTGAACCAGA AGAAGAAACA GACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Oxysterol gradient generation by lymphoid stromal cells guides activated B cell movement during humoral responses.
Immunity37(3)535-48(2012 Sep)
Yi T,Wang X,Kelly LM,An J,Xu Y,Sailer AW,Gustafsson JA,Russell DW,Cyster JG

Testosterone regulates 25-hydroxycholesterol production in testicular macrophages.
Biology of reproduction67(5)1435-8(2002 Nov)
Lukyanenko Y,Chen JJ,Hutson JC

25-hydroxycholesterol is produced by testicular macrophages during the early postnatal period and influences differentiation of Leydig cells in vitro.
Biology of reproduction66(5)1336-41(2002 May)
Chen JJ,Lukyanenko Y,Hutson JC

Production of 25-hydroxycholesterol by testicular macrophages and its effects on Leydig cells.
Biology of reproduction64(3)790-6(2001 Mar)
Lukyanenko YO,Chen JJ,Hutson JC

cDNA cloning of mouse and human cholesterol 25-hydroxylases, polytopic membrane proteins that synthesize a potent oxysterol regulator of lipid metabolism.
The Journal of biological chemistry273(51)34316-27(1998 Dec)
Lund EG,Kerr TA,Sakai J,Li WP,Russell DW