Zfyve27 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Zfyve27
Entrez Gene ID	309376
Full Name	zinc finger FYVE-type containing 27
General protein information	Preferred Names zinc finger FYVE-type containing 27 Names protrudin zinc finger FYVE domain-containing protein 27 zinc finger, FYVE domain containing 27
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q54

mRNA and Protein(s)

mRNA	Protein	Name
XM_006231392.3	XP_006231454.1	protrudin isoform X1
NM_199104.2	NP_954535.2	protrudin

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	ZFYVE27	XP_001165164.2
	ZFYVE27	XP_001105442.1
Rattus norvegicus (Norway rat)	Zfyve27	NP_954535.2
Danio rerio (zebrafish)	zfyve27	NP_001070759.2
Canis lupus familiaris (dog)	ZFYVE27	XP_543954.2
Mus musculus (house mouse)	Zfyve27	NP_796293.2
Homo sapiens (human)	ZFYVE27	NP_001002261.1
Bos taurus (cattle)	ZFYVE27	NP_001017939.1
Gallus gallus (chicken)	ZFYVE27	NP_001034393.1
Xenopus tropicalis (tropical clawed frog)	zfyve27	NP_001116926.1

Gene Ontology
Bibliography

Related articles in PubMed

Protrudin regulates endoplasmic reticulum morphology and function associated with the pathogenesis of hereditary spastic paraplegia.
Hashimoto Y, Shirane M, Matsuzaki F, Saita S, Ohnishi T, Nakayama KI
The Journal of biological chemistry289(19)12946-61(2014 May)

Identification and characterization of a neuron-specific isoform of protrudin.
Ohnishi T, Shirane M, Hashimoto Y, Saita S, Nakayama KI
Genes to cells : devoted to molecular & cellular mechanisms19(2)97-111(2014 Feb)

Protrudin binds atlastins and endoplasmic reticulum-shaping proteins and regulates network formation.
Chang J, Lee S, Blackstone C
Proceedings of the National Academy of Sciences of the United States of America110(37)14954-9(2013 Sep)

Protrudin serves as an adaptor molecule that connects KIF5 and its cargoes in vesicular transport during process formation.
Matsuzaki F, Shirane M, Matsumoto M, Nakayama KI
Molecular biology of the cell22(23)4602-20(2011 Dec)

Promotion of neurite extension by protrudin requires its interaction with vesicle-associated membrane protein-associated protein.
Saita S, Shirane M, Natume T, Iemura S, Nakayama KI
The Journal of biological chemistry284(20)13766-77(2009 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

VAP-A is an important regulator both of the subcellular localization of protrudin and of its ability to stimulate neurite outgrowth.
Title: Promotion of neurite extension by protrudin requires its interaction with vesicle-associated membrane protein-associated protein.

protrudin regulates Rab11-dependent membrane recycling to promote the directional membrane trafficking required for neurite formation [protrudin]
Title: Protrudin induces neurite formation by directional membrane trafficking.

The following Zfyve27 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Zfyve27 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa05035	NM_199104.2 Latest version!	Rattus norvegicus zinc finger FYVE-type containing 27 (Zfyve27), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa24088	XM_006231392.3 Latest version!	Rattus norvegicus zinc finger FYVE-type containing 27 (Zfyve27), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Zfyve27 ( NM_199104.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_954535.2 Rattus norvegicus zinc finger FYVE-type containing 27 (Zfyve27), mRNA.

Price & Availability↑

CloneID	ORa05035
Clone ID Related Accession (Same CDS sequence)	NM_199104.2
Accession Version	NM_199104.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1215bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-09-21
Organism	Rattus norvegicus(Norway rat)
Product	protrudin
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07006870.1. On Aug 31, 2012 this sequence version replaced NM_199104.1. Sequence Note: This RefSeq record was created from genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BC061741.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGCAGTCTT CGGATCGGGA CCTGAGTGGA CCAGAGGCGA GCCCCAGCGT GATGCCTGAG 
GTTCTCTCTG AGTGTTCACC TGCCCCTACC AAGTCAACAG CGTTTGATCT TTTCAACCTG 
GTTCTGTCCT ACAAGAGGCT GGAGATCTAC CTGGAACCCC TGAAGGATGC AGGTGACGGT 
GTTCGATACT TGCTAAGGTG GCAGATGCCT TTGTGTTCCT TGCTGACTTG CCTGGGCCTC 
AACATCTTGT TCCTCACTCT GAATGAGGGT GCATGGTACT CCGTGGGTGC CTTGATAATT 
TCGGTGCCTG CCCTACTGGG CTACCTTCAG GAGGTGTGCC GGGCACAGCT ACCAGAGTCT 
GAGCTGATGA GGCGGAAGTA CCACAGCGTG CGGCAGGAAG ACCTGCAGAG AGTTCGCCTT 
TCCCGCCCTG AGGCTGTAGC TGAGGTGAAA AGCTTCTTGA TCCGACTGGA AGCCTTCTTG 
GCCCGCCTGT GCTATACCTG CGAGTCAGCT TACCGTGTAC TTCACTGGGA GAACCCTGTG 
GTGTCCTCAC AGTTCTATGG TGCTCTTCTG GGCATGGTTT GCATGCTCTA CCTGCTGCCG 
CTCTGCTGGG TCCTCGCCCT TTTAAACAGC ACACTCTTTC TGGGAAATGG GGAATTCTTC 
CGAGTGGTGT CTGAGTACAG GGCTTGTCTG CAGCGGCGGA TGAGCCCCAA GCAGGAAGAG 
TGCGTCTGTG AGGGCTCGGC ACTGCAGGAT GCCGGGGGGA GGGCTGTTGT ACTGGACAGC 
ACCCCTGCCC CTACACCCAC AGAGGACCTC ACGCCAGGCA GTGTGGAGGA AGCTGAGGAG 
GCTGAGCCAG ATGAGGAGTT CAAAGATGCA ATAGAGGAGG ACGATGAGGG AACCCCATGC 
CCAGCAGAGG ATGAGCTGAC CATGCAGGAC AATGGCTTCC TCAGCAAGAA TGAGGTACTG 
CGCAGCAAGG TGTCGAAGCT TACAGAGCGG CTCCGCAAGC GCTACCCGAC CAACAACTTC 
GGGAACTGTG CAGGCTGTGC TGCCACCTTC TCCGTGCTGA AGAAGAGGCG AAGCTGCAGC 
AACTGTGGGA ACAGCTTCTG CTCTCGGTGC TGCTCCTTCA AGGTGCCCAA GTCCTCCATG 
GGGGCCACAG CTCCTGAAGC CCAGAGAGAG ACTGTGTTTG TGTGTGCCTC CTGTAATCAG 
ACCTTGAGCA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_954535.2
CDS	187..1401
Translation	MQSSDRDLSGPEASPSVMPEVLSECSPAPTKSTAFDLFNLVLSYKRLEIYLEPLKDAGDGVRYLLRWQMPLCSLLTCLGLNILFLTLNEGAWYSVGALIISVPALLGYLQEVCRAQLPESELMRRKYHSVRQEDLQRVRLSRPEAVAEVKSFLIRLEAFLARLCYTCESAYRVLHWENPVVSSQFYGALLGMVCMLYLLPLCWVLALLNSTLFLGNGEFFRVVSEYRACLQRRMSPKQEECVCEGSALQDAGGRAVVLDSTPAPTPTEDLTPGSVEEAEEAEPDEEFKDAIEEDDEGTPCPAEDELTMQDNGFLSKNEVLRSKVSKLTERLRKRYPTNNFGNCAGCAATFSVLKKRRSCSNCGNSFCSRCCSFKVPKSSMGATAPEAQRETVFVCASCNQTLSK

Target ORF information:

RefSeq Version	NM_199104.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus zinc finger FYVE-type containing 27 (Zfyve27), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_199104.2

ORF Insert Sequence:

ATGCAGTCTT CGGATCGGGA CCTGAGTGGA CCAGAGGCGA GCCCCAGCGT GATGCCTGAG 
GTTCTCTCTG AGTGTTCACC TGCCCCTACC AAGTCAACAG CGTTTGATCT TTTCAACCTG 
GTTCTGTCCT ACAAGAGGCT GGAGATCTAC CTGGAACCCC TGAAGGATGC AGGTGACGGT 
GTTCGATACT TGCTAAGGTG GCAGATGCCT TTGTGTTCCT TGCTGACTTG CCTGGGCCTC 
AACATCTTGT TCCTCACTCT GAATGAGGGT GCATGGTACT CCGTGGGTGC CTTGATAATT 
TCGGTGCCTG CCCTACTGGG CTACCTTCAG GAGGTGTGCC GGGCACAGCT ACCAGAGTCT 
GAGCTGATGA GGCGGAAGTA CCACAGCGTG CGGCAGGAAG ACCTGCAGAG AGTTCGCCTT 
TCCCGCCCTG AGGCTGTAGC TGAGGTGAAA AGCTTCTTGA TCCGACTGGA AGCCTTCTTG 
GCCCGCCTGT GCTATACCTG CGAGTCAGCT TACCGTGTAC TTCACTGGGA GAACCCTGTG 
GTGTCCTCAC AGTTCTATGG TGCTCTTCTG GGCATGGTTT GCATGCTCTA CCTGCTGCCG 
CTCTGCTGGG TCCTCGCCCT TTTAAACAGC ACACTCTTTC TGGGAAATGG GGAATTCTTC 
CGAGTGGTGT CTGAGTACAG GGCTTGTCTG CAGCGGCGGA TGAGCCCCAA GCAGGAAGAG 
TGCGTCTGTG AGGGCTCGGC ACTGCAGGAT GCCGGGGGGA GGGCTGTTGT ACTGGACAGC 
ACCCCTGCCC CTACACCCAC AGAGGACCTC ACGCCAGGCA GTGTGGAGGA AGCTGAGGAG 
GCTGAGCCAG ATGAGGAGTT CAAAGATGCA ATAGAGGAGG ACGATGAGGG AACCCCATGC 
CCAGCAGAGG ATGAGCTGAC CATGCAGGAC AATGGCTTCC TCAGCAAGAA TGAGGTACTG 
CGCAGCAAGG TGTCGAAGCT TACAGAGCGG CTCCGCAAGC GCTACCCGAC CAACAACTTC 
GGGAACTGTG CAGGCTGTGC TGCCACCTTC TCCGTGCTGA AGAAGAGGCG AAGCTGCAGC 
AACTGTGGGA ACAGCTTCTG CTCTCGGTGC TGCTCCTTCA AGGTGCCCAA GTCCTCCATG 
GGGGCCACAG CTCCTGAAGC CCAGAGAGAG ACTGTGTTTG TGTGTGCCTC CTGTAATCAG 
ACCTTGAGCA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Zfyve27 ( XM_006231392.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006231454.1 Rattus norvegicus zinc finger FYVE-type containing 27 (Zfyve27), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa24088
Clone ID Related Accession (Same CDS sequence)	XM_006231392.3
Accession Version	XM_006231392.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1236bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	protrudin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006231392.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGTCTT CGGATCGGGA CCTGAGTGGA CCAGAGGCGA GCCCCAGCGT GATGCCTGAG 
GTTCTCTCTG AGTGTTCACC TGCCCCTACC AAGTCAACAG CGTTTGATCT TTTCAACCTG 
GTTCTGTCCT ACAAGAGGCT GGAGATCTAC CTGGAACCCC TGAAGGATGC AGGTGACGGT 
GTTCGATACT TGCTAAGGTG GCAGATGCCT TTGTGTTCCT TGCTGACTTG CCTGGGCCTC 
AACATCTTGT TCCTCACTCT GAATGAGGGT GCATGGTACT CCGTGGGTGC CTTGATAATT 
TCGGTGCCTG CCCTACTGGG CTACCTTCAG GAGGTGTGCC GGGCACAGCT ACCAGAGTCT 
GAGCTGATGA GGCGGAAGTA CCACAGCGTG CGGCAGGAAG ACCTGCAGAG AGTTCGCCTT 
TCCCGCCCTG AGGCTGTAGC TGAGGTGAAA AGCTTCTTGA TCCGACTGGA AGCCTTCTTG 
GCCCGCCTGT GCTATACCTG CGAGTCAGCT TACCGTGTAC TTCACTGGGA GAACCCTGTG 
GTGTCCTCAC AGTTCTATGG TGCTCTTCTG GGCATGGTTT GCATGCTCTA CCTGCTGCCG 
CTCTGCTGGG TCCTCGCCCT TTTAAACAGC ACACTCTTTC TGGGAAATGG GGAATTCTTC 
CGAGTGGTGT CTGAGTACAG GGCTTGTCTG CAGCGGCGGA TGAGCCCCAA GCAGGAAGAG 
TGCGTCTGTG AGGGCTCGGC ACTGCAGGAT GCCGGGGGGA GGGCTGTTGT ACTGGACAGC 
ACCCCTGCCC CTACACCCAC AGAGGACCTC ACGCCAGGCA GTGTGGAGGA AGCTGAGGAG 
GCTGAGCCAG ATGAGGAGTT CAAAGATGCA ATAGAGGAGA CCCACCTGGT GGTGCTGGAG 
GACGATGAGG GAACCCCATG CCCAGCAGAG GATGAGCTGA CCATGCAGGA CAATGGCTTC 
CTCAGCAAGA ATGAGGTACT GCGCAGCAAG GTGTCGAAGC TTACAGAGCG GCTCCGCAAG 
CGCTACCCGA CCAACAACTT CGGGAACTGT GCAGGCTGTG CTGCCACCTT CTCCGTGCTG 
AAGAAGAGGC GAAGCTGCAG CAACTGTGGG AACAGCTTCT GCTCTCGGTG CTGCTCCTTC 
AAGGTGCCCA AGTCCTCCAT GGGGGCCACA GCTCCTGAAG CCCAGAGAGA GACTGTGTTT 
GTGTGTGCCT CCTGTAATCA GACCTTGAGC AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006231454.1
CDS	206..1441
Translation	MQSSDRDLSGPEASPSVMPEVLSECSPAPTKSTAFDLFNLVLSYKRLEIYLEPLKDAGDGVRYLLRWQMPLCSLLTCLGLNILFLTLNEGAWYSVGALIISVPALLGYLQEVCRAQLPESELMRRKYHSVRQEDLQRVRLSRPEAVAEVKSFLIRLEAFLARLCYTCESAYRVLHWENPVVSSQFYGALLGMVCMLYLLPLCWVLALLNSTLFLGNGEFFRVVSEYRACLQRRMSPKQEECVCEGSALQDAGGRAVVLDSTPAPTPTEDLTPGSVEEAEEAEPDEEFKDAIEETHLVVLEDDEGTPCPAEDELTMQDNGFLSKNEVLRSKVSKLTERLRKRYPTNNFGNCAGCAATFSVLKKRRSCSNCGNSFCSRCCSFKVPKSSMGATAPEAQRETVFVCASCNQTLSK

Target ORF information:

RefSeq Version	XM_006231392.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus zinc finger FYVE-type containing 27 (Zfyve27), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006231392.3

ORF Insert Sequence:

ATGCAGTCTT CGGATCGGGA CCTGAGTGGA CCAGAGGCGA GCCCCAGCGT GATGCCTGAG 
GTTCTCTCTG AGTGTTCACC TGCCCCTACC AAGTCAACAG CGTTTGATCT TTTCAACCTG 
GTTCTGTCCT ACAAGAGGCT GGAGATCTAC CTGGAACCCC TGAAGGATGC AGGTGACGGT 
GTTCGATACT TGCTAAGGTG GCAGATGCCT TTGTGTTCCT TGCTGACTTG CCTGGGCCTC 
AACATCTTGT TCCTCACTCT GAATGAGGGT GCATGGTACT CCGTGGGTGC CTTGATAATT 
TCGGTGCCTG CCCTACTGGG CTACCTTCAG GAGGTGTGCC GGGCACAGCT ACCAGAGTCT 
GAGCTGATGA GGCGGAAGTA CCACAGCGTG CGGCAGGAAG ACCTGCAGAG AGTTCGCCTT 
TCCCGCCCTG AGGCTGTAGC TGAGGTGAAA AGCTTCTTGA TCCGACTGGA AGCCTTCTTG 
GCCCGCCTGT GCTATACCTG CGAGTCAGCT TACCGTGTAC TTCACTGGGA GAACCCTGTG 
GTGTCCTCAC AGTTCTATGG TGCTCTTCTG GGCATGGTTT GCATGCTCTA CCTGCTGCCG 
CTCTGCTGGG TCCTCGCCCT TTTAAACAGC ACACTCTTTC TGGGAAATGG GGAATTCTTC 
CGAGTGGTGT CTGAGTACAG GGCTTGTCTG CAGCGGCGGA TGAGCCCCAA GCAGGAAGAG 
TGCGTCTGTG AGGGCTCGGC ACTGCAGGAT GCCGGGGGGA GGGCTGTTGT ACTGGACAGC 
ACCCCTGCCC CTACACCCAC AGAGGACCTC ACGCCAGGCA GTGTGGAGGA AGCTGAGGAG 
GCTGAGCCAG ATGAGGAGTT CAAAGATGCA ATAGAGGAGA CCCACCTGGT GGTGCTGGAG 
GACGATGAGG GAACCCCATG CCCAGCAGAG GATGAGCTGA CCATGCAGGA CAATGGCTTC 
CTCAGCAAGA ATGAGGTACT GCGCAGCAAG GTGTCGAAGC TTACAGAGCG GCTCCGCAAG 
CGCTACCCGA CCAACAACTT CGGGAACTGT GCAGGCTGTG CTGCCACCTT CTCCGTGCTG 
AAGAAGAGGC GAAGCTGCAG CAACTGTGGG AACAGCTTCT GCTCTCGGTG CTGCTCCTTC 
AAGGTGCCCA AGTCCTCCAT GGGGGCCACA GCTCCTGAAG CCCAGAGAGA GACTGTGTTT 
GTGTGTGCCT CCTGTAATCA GACCTTGAGC AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Protrudin regulates endoplasmic reticulum morphology and function associated with the pathogenesis of hereditary spastic paraplegia.
The Journal of biological chemistry289(19)12946-61(2014 May)
Hashimoto Y,Shirane M,Matsuzaki F,Saita S,Ohnishi T,Nakayama KI

Identification and characterization of a neuron-specific isoform of protrudin.
Genes to cells : devoted to molecular & cellular mechanisms19(2)97-111(2014 Feb)
Ohnishi T,Shirane M,Hashimoto Y,Saita S,Nakayama KI

Protrudin binds atlastins and endoplasmic reticulum-shaping proteins and regulates network formation.
Proceedings of the National Academy of Sciences of the United States of America110(37)14954-9(2013 Sep)
Chang J,Lee S,Blackstone C

Protrudin serves as an adaptor molecule that connects KIF5 and its cargoes in vesicular transport during process formation.
Molecular biology of the cell22(23)4602-20(2011 Dec)
Matsuzaki F,Shirane M,Matsumoto M,Nakayama KI

Promotion of neurite extension by protrudin requires its interaction with vesicle-associated membrane protein-associated protein.
The Journal of biological chemistry284(20)13766-77(2009 May)
Saita S,Shirane M,Natume T,Iemura S,Nakayama KI