Sh3rf2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sh3rf2
Entrez Gene ID	307472
Full Name	SH3 domain containing ring finger 2
Synonyms	Posh2,Ppp1r39
General protein information	Preferred Names SH3 domain containing ring finger 2 Names putative E3 ubiquitin-protein ligase SH3RF2 RING finger protein 158 RING-type E3 ubiquitin transferase SH3RF2 SH3 domain-containing RING finger protein 2 protein phosphatase 1 regulatory subunit 39
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 18 Map Location: 18p11

mRNA and Protein(s)

mRNA	Protein	Name
NM_001034187.1	NP_001029359.1	E3 ubiquitin-protein ligase SH3RF2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	SH3RF2	XP_003310936.1
	SH3RF2	XP_001099248.1
Mus musculus (house mouse)	Sh3rf2	NP_001139771.1
Xenopus tropicalis (tropical clawed frog)	sh3rf2	XP_002935311.1
Canis lupus familiaris (dog)	SH3RF2	XP_858579.1
Gallus gallus (chicken)	SH3RF2	XP_414662.3
Homo sapiens (human)	SH3RF2	NP_689763.3
Rattus norvegicus (Norway rat)	Sh3rf2	NP_001029359.1
Bos taurus (cattle)	PPP1R39	NP_001077222.1

Gene Ontology
Bibliography

Related articles in PubMed

SH3RF2 functions as an oncogene by mediating PAK4 protein stability.
Kim TW, Kang YK, Park ZY, Kim YH, Hong SW, Oh SJ, Sohn HA, Yang SJ, Jang YJ, Lee DC, Kim SY, Yoo HS, Kim E, Yeom YI, Park KC
Carcinogenesis35(3)624-34(2014 Mar)

Sh3rf2/POSHER protein promotes cell survival by ring-mediated proteasomal degradation of the c-Jun N-terminal kinase scaffold POSH (Plenty of SH3s) protein.
Wilhelm M, Kukekov NV, Schmit TL, Biagas KV, Sproul AA, Gire S, Maes ME, Xu Z, Greene LA
The Journal of biological chemistry287(3)2247-56(2012 Jan)

FLJ23654 encodes a heart protein phosphatase 1-binding protein (Hepp1).
Chen CY, Lai NS, Yang JJ, Huang HL, Hung WC, Li C, Lin TH, Huang HB
Biochemical and biophysical research communications391(1)698-702(2010 Jan)

Docking motif-guided mapping of the interactome of protein phosphatase-1.
Hendrickx A, Beullens M, Ceulemans H, Den Abt T, Van Eynde A, Nicolaescu E, Lesage B, Bollen M
Chemistry & biology16(4)365-71(2009 Apr)

Identification of POSH2, a novel homologue of the c-Jun N-terminal kinase scaffold protein POSH.
Wilhelm M, Kukekov NV, Xu Z, Greene LA
Developmental neuroscience29(4-5)355-62(2007)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

a model in which Sh3rf2 promotes proteasomal degradation of pro-apoptotic POSH in healthy cells and in which apoptotic stimuli lead to rapid loss of Sh3rf2 expression, and consequently to stabilization of POSH and JNK activation and cell death
Title: Sh3rf2/POSHER protein promotes cell survival by ring-mediated proteasomal degradation of the c-Jun N-terminal kinase scaffold POSH (Plenty of SH3s) protein.

These results indicate that POSH2 may regulate JNK activation and consequent apoptosis under conditions of increased expression.
Title: Identification of POSH2, a novel homologue of the c-Jun N-terminal kinase scaffold protein POSH.

The following Sh3rf2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sh3rf2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa08384	NM_001034187.1 Latest version!	Rattus norvegicus SH3 domain containing ring finger 2 (Sh3rf2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sh3rf2 ( NM_001034187.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001029359.1 Rattus norvegicus SH3 domain containing ring finger 2 (Sh3rf2), mRNA.

Price & Availability↑

CloneID	ORa08384
Clone ID Related Accession (Same CDS sequence)	NM_001034187.1
Accession Version	NM_001034187.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2208bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-18
Organism	Rattus norvegicus(Norway rat)
Product	E3 ubiquitin-protein ligase SH3RF2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC100155.1. On Oct 17, 2005 this sequence version replaced XM_225981.3. ##Evidence-Data-START## Transcript exon combination :: BC100155.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689599, SAMEA2689602 [ECO:0000348] ##Evidence-Data-END##

ATGGATGATT TGACGTTACT TGATCTCCTG GAGTGCCCCG TGTGCTTTGA AAAGCTCGAT 
GTTACAGCCA AAGTTCTCCC CTGCCAGCAC ACCTTCTGCA AACCATGTCT ACAGCGGATT 
TTCAAGGCCC ACAAGGAGCT GAGGTGCCCG GAATGCAGGA CCCTGGTGTT CTGCAGCATC 
GAGGCTCTGC CAGCCAACCT GTTGCTGGTG CGTCTGCTGG ATGGAGTGCG CTCCGGGCAC 
AACTCGTGGA GAGGAGGCTC CTTTCGAAGG CCTCGAATAC TAACCTTGCA GGACAACAGG 
AAAGCCAAGA GCAGCCCCAG AAGTCTGCAG GCCAGCCCTT TCCGGCTGGG GCCCACTGTC 
AGGATCCACA TGGATGGGGT GCCTCGAGCC AAGGCCTTGT GCAACTACAG AGGGAAGAAT 
CCTGGCGACC TGAAGTTTAA TAAAGGAGAT GTCATCCTCC TTCAGAGACA GCTTGATGAG 
AACTGGTACC AGGGGGAGAT CAATGGGGTC AGTGGGTTCT TCCCAGCCAG CTCTGTGGAA 
GTCATCAAGC AGCTGCCCCA GCCACCCCCT CTCTGCAGAG CCCTCTACAA CTTTGACCTT 
CGAGATAAGG ATAAGAGTGA GAACCAGGAT TGCCTGACCT TCCTCAAGGA TGATGTCATC 
ACAGTGATCA GCCGAGTAGA CGAGAACTGG GCAGAAGGAA AACTCGGAGA TAAAGTAGGC 
ATCTTCCCCA TCTTGTTTGT AGAGCCAAAC CTCTCTGCAA GACACCTCCT AGAGAAGAGC 
AAAGGGCATC AGTTATCACG CACAAAACAT CTGTCCCTGA TGTCCTCACC ATCTAGAGGC 
AAAGCAACCA ACACATCCAC TCTCCGCAAG AGCCCAGGGT CCAGGAGGAA AGGATCCGGG 
CAGTTCGCCA TGACTACAGC TTTGAATACA CTCAACCGCA TGGTCCATTC TCCTGAAGGT 
CACCAGATGG TGGAAATCAG CACCCCAGTG CTCATCAGCT CTACCAGTCC CTCTATGCTC 
ACCCAGCATG GGGACAGGGC CGATTTCCCT GCCAGCTCTG CAGGACAGGT CAGCACTTCT 
CACCCTGCTC CTGCCTCCCC AGGACATTCC ACAGCCATGG TCAGTGTGCC CAGCTCCCAG 
CAACACCTCT CAACGAACAT GTTTGTAGCC CTGCACACTT ACTCAGCCCA GGGACCTGAA 
GAGCTGGACC TGAAGAAGGG AGAAGGTATC AGGGTTCTGG GCAAGAATCA GGATGGCTGG 
CTGAGGGGCG TCTCCTTAGT CACTGGACGA ACTGGCATCT TTCCAAGCGA CTATGTCATC 
CCCGTTTTCA GCTCAACAGC TAGAAAGACA TCTAGTTTTC CTGATTCCCG GCACCCCACT 
GTCTGCACCA CATGGGCGTT ATCCACCTCC TCTGTGTCCT CCCAGGGTAG CTTTTCAGAA 
GGCGATCCCC GGCAAAGTGG ACCCTTCAGA TCTGTCTTTG TGCCCACTGC CGTTAACCCT 
CCAAGGAGCA CATCTGGCCC TGGGACTTCT GGACAAGGGT CTCTTAGGAA AGTGCGGAGC 
AGCATGAGAA AGAATGGATC CCTGCAGAGA CCAGTTCAAT CAGGGATCCC CACTTTCATG 
GTGGGCTCCC TCAGGTGCAG TCCTGCCATG GTGATCCGGC CTCAGAAGTT CCAGTTTTAT 
CAGCCACAGG GGATGACCCC TTCCCCAACA CCCATAATGG TGGAGATAGG GTCTAAATCT 
ATTTCCACTG GGGAGCCTGC CCTGACCTGC ATCAACAGAG GTGGCAAGAC CAGGACCCAC 
TCCGCAGGCA ATTCCATCAT CATGGAAGGC AAAGAAACAC CCATCAAGAG TGAGCCTCCA 
CCCAAGCCGC CTGCGTCTGC ACCACCCTCC ATCCTCGTGA AACCAGAAAA TTCAAAAAAT 
GGTATTGAAA AGCAAGTCAA AACCGTGAGA TTTCAGAATT ACAGCCCTCC GCCCACCAAA 
CACTCTGCCT CCGGTCCCAC CTCTGGAAAG CATGAACAGC CAGCCACCTT GAAGGGGTCC 
CAACCTGAAG CAGTCTCCTC AGAAGGAGAG ATGACCATTC TATTTGCCCA CCGAAGTGGC 
TGCCACTCCG GGCAACAGAC AGACCTTCGG AGAAAGTCAG CCTTTAGCAA GACCACACCC 
CCAGTGTCCA CTGCTTCAGT CTCACAGACC TTATTTCCCA GCAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001029359.1
CDS	146..2353
Translation	MDDLTLLDLLECPVCFEKLDVTAKVLPCQHTFCKPCLQRIFKAHKELRCPECRTLVFCSIEALPANLLLVRLLDGVRSGHNSWRGGSFRRPRILTLQDNRKAKSSPRSLQASPFRLGPTVRIHMDGVPRAKALCNYRGKNPGDLKFNKGDVILLQRQLDENWYQGEINGVSGFFPASSVEVIKQLPQPPPLCRALYNFDLRDKDKSENQDCLTFLKDDVITVISRVDENWAEGKLGDKVGIFPILFVEPNLSARHLLEKSKGHQLSRTKHLSLMSSPSRGKATNTSTLRKSPGSRRKGSGQFAMTTALNTLNRMVHSPEGHQMVEISTPVLISSTSPSMLTQHGDRADFPASSAGQVSTSHPAPASPGHSTAMVSVPSSQQHLSTNMFVALHTYSAQGPEELDLKKGEGIRVLGKNQDGWLRGVSLVTGRTGIFPSDYVIPVFSSTARKTSSFPDSRHPTVCTTWALSTSSVSSQGSFSEGDPRQSGPFRSVFVPTAVNPPRSTSGPGTSGQGSLRKVRSSMRKNGSLQRPVQSGIPTFMVGSLRCSPAMVIRPQKFQFYQPQGMTPSPTPIMVEIGSKSISTGEPALTCINRGGKTRTHSAGNSIIMEGKETPIKSEPPPKPPASAPPSILVKPENSKNGIEKQVKTVRFQNYSPPPTKHSASGPTSGKHEQPATLKGSQPEAVSSEGEMTILFAHRSGCHSGQQTDLRRKSAFSKTTPPVSTASVSQTLFPSK

Target ORF information:

RefSeq Version	NM_001034187.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus SH3 domain containing ring finger 2 (Sh3rf2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001034187.1

ORF Insert Sequence:

ATGGATGATT TGACGTTACT TGATCTCCTG GAGTGCCCCG TGTGCTTTGA AAAGCTCGAT 
GTTACAGCCA AAGTTCTCCC CTGCCAGCAC ACCTTCTGCA AACCATGTCT ACAGCGGATT 
TTCAAGGCCC ACAAGGAGCT GAGGTGCCCG GAATGCAGGA CCCTGGTGTT CTGCAGCATC 
GAGGCTCTGC CAGCCAACCT GTTGCTGGTG CGTCTGCTGG ATGGAGTGCG CTCCGGGCAC 
AACTCGTGGA GAGGAGGCTC CTTTCGAAGG CCTCGAATAC TAACCTTGCA GGACAACAGG 
AAAGCCAAGA GCAGCCCCAG AAGTCTGCAG GCCAGCCCTT TCCGGCTGGG GCCCACTGTC 
AGGATCCACA TGGATGGGGT GCCTCGAGCC AAGGCCTTGT GCAACTACAG AGGGAAGAAT 
CCTGGCGACC TGAAGTTTAA TAAAGGAGAT GTCATCCTCC TTCAGAGACA GCTTGATGAG 
AACTGGTACC AGGGGGAGAT CAATGGGGTC AGTGGGTTCT TCCCAGCCAG CTCTGTGGAA 
GTCATCAAGC AGCTGCCCCA GCCACCCCCT CTCTGCAGAG CCCTCTACAA CTTTGACCTT 
CGAGATAAGG ATAAGAGTGA GAACCAGGAT TGCCTGACCT TCCTCAAGGA TGATGTCATC 
ACAGTGATCA GCCGAGTAGA CGAGAACTGG GCAGAAGGAA AACTCGGAGA TAAAGTAGGC 
ATCTTCCCCA TCTTGTTTGT AGAGCCAAAC CTCTCTGCAA GACACCTCCT AGAGAAGAGC 
AAAGGGCATC AGTTATCACG CACAAAACAT CTGTCCCTGA TGTCCTCACC ATCTAGAGGC 
AAAGCAACCA ACACATCCAC TCTCCGCAAG AGCCCAGGGT CCAGGAGGAA AGGATCCGGG 
CAGTTCGCCA TGACTACAGC TTTGAATACA CTCAACCGCA TGGTCCATTC TCCTGAAGGT 
CACCAGATGG TGGAAATCAG CACCCCAGTG CTCATCAGCT CTACCAGTCC CTCTATGCTC 
ACCCAGCATG GGGACAGGGC CGATTTCCCT GCCAGCTCTG CAGGACAGGT CAGCACTTCT 
CACCCTGCTC CTGCCTCCCC AGGACATTCC ACAGCCATGG TCAGTGTGCC CAGCTCCCAG 
CAACACCTCT CAACGAACAT GTTTGTAGCC CTGCACACTT ACTCAGCCCA GGGACCTGAA 
GAGCTGGACC TGAAGAAGGG AGAAGGTATC AGGGTTCTGG GCAAGAATCA GGATGGCTGG 
CTGAGGGGCG TCTCCTTAGT CACTGGACGA ACTGGCATCT TTCCAAGCGA CTATGTCATC 
CCCGTTTTCA GCTCAACAGC TAGAAAGACA TCTAGTTTTC CTGATTCCCG GCACCCCACT 
GTCTGCACCA CATGGGCGTT ATCCACCTCC TCTGTGTCCT CCCAGGGTAG CTTTTCAGAA 
GGCGATCCCC GGCAAAGTGG ACCCTTCAGA TCTGTCTTTG TGCCCACTGC CGTTAACCCT 
CCAAGGAGCA CATCTGGCCC TGGGACTTCT GGACAAGGGT CTCTTAGGAA AGTGCGGAGC 
AGCATGAGAA AGAATGGATC CCTGCAGAGA CCAGTTCAAT CAGGGATCCC CACTTTCATG 
GTGGGCTCCC TCAGGTGCAG TCCTGCCATG GTGATCCGGC CTCAGAAGTT CCAGTTTTAT 
CAGCCACAGG GGATGACCCC TTCCCCAACA CCCATAATGG TGGAGATAGG GTCTAAATCT 
ATTTCCACTG GGGAGCCTGC CCTGACCTGC ATCAACAGAG GTGGCAAGAC CAGGACCCAC 
TCCGCAGGCA ATTCCATCAT CATGGAAGGC AAAGAAACAC CCATCAAGAG TGAGCCTCCA 
CCCAAGCCGC CTGCGTCTGC ACCACCCTCC ATCCTCGTGA AACCAGAAAA TTCAAAAAAT 
GGTATTGAAA AGCAAGTCAA AACCGTGAGA TTTCAGAATT ACAGCCCTCC GCCCACCAAA 
CACTCTGCCT CCGGTCCCAC CTCTGGAAAG CATGAACAGC CAGCCACCTT GAAGGGGTCC 
CAACCTGAAG CAGTCTCCTC AGAAGGAGAG ATGACCATTC TATTTGCCCA CCGAAGTGGC 
TGCCACTCCG GGCAACAGAC AGACCTTCGG AGAAAGTCAG CCTTTAGCAA GACCACACCC 
CCAGTGTCCA CTGCTTCAGT CTCACAGACC TTATTTCCCA GCAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

SH3RF2 functions as an oncogene by mediating PAK4 protein stability.
Carcinogenesis35(3)624-34(2014 Mar)
Kim TW,Kang YK,Park ZY,Kim YH,Hong SW,Oh SJ,Sohn HA,Yang SJ,Jang YJ,Lee DC,Kim SY,Yoo HS,Kim E,Yeom YI,Park KC

Sh3rf2/POSHER protein promotes cell survival by ring-mediated proteasomal degradation of the c-Jun N-terminal kinase scaffold POSH (Plenty of SH3s) protein.
The Journal of biological chemistry287(3)2247-56(2012 Jan)
Wilhelm M,Kukekov NV,Schmit TL,Biagas KV,Sproul AA,Gire S,Maes ME,Xu Z,Greene LA

FLJ23654 encodes a heart protein phosphatase 1-binding protein (Hepp1).
Biochemical and biophysical research communications391(1)698-702(2010 Jan)
Chen CY,Lai NS,Yang JJ,Huang HL,Hung WC,Li C,Lin TH,Huang HB

Docking motif-guided mapping of the interactome of protein phosphatase-1.
Chemistry & biology16(4)365-71(2009 Apr)
Hendrickx A,Beullens M,Ceulemans H,Den Abt T,Van Eynde A,Nicolaescu E,Lesage B,Bollen M

Identification of POSH2, a novel homologue of the c-Jun N-terminal kinase scaffold protein POSH.
Developmental neuroscience29(4-5)355-62(2007)
Wilhelm M,Kukekov NV,Xu Z,Greene LA