Ccar2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ccar2
Entrez Gene ID	306007
Full Name	cell cycle and apoptosis regulator 2
Synonyms	RGD1309922
General protein information	Preferred Names cell cycle and apoptosis regulator 2 Names cell cycle and apoptosis regulator protein 2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 15 Map Location: 15p11

mRNA and Protein(s)

mRNA	Protein	Name
XM_006252268.3	XP_006252330.1	cell cycle and apoptosis regulator protein 2 isoform X1
XM_017599702.1	XP_017455191.1	cell cycle and apoptosis regulator protein 2 isoform X2
NM_001170472.1	NP_001163943.1	cell cycle and apoptosis regulator protein 2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	KIAA1967	XP_003311685.1
Canis lupus familiaris (dog)	KIAA1967	XP_005635766.1
Rattus norvegicus (Norway rat)	Ccar2	NP_001163943.1
Homo sapiens (human)	CCAR2	NP_066997.3
Bos taurus (cattle)	KIAA1967	NP_001068766.2
Mus musculus (house mouse)	Ccar2	NP_666167.3
	KIAA1967	XP_001106923.2

Gene Ontology
Bibliography

Related articles in PubMed

CCAR2 negatively regulates nuclear receptor LXR? by competing with SIRT1 deacetylase.
Sakurabashi A, Wada-Hiraike O, Hirano M, Fu H, Isono W, Fukuda T, Morita Y, Tanikawa M, Miyamoto Y, Oda K, Kawana K, Osuga Y, Fujii T
The Journal of steroid biochemistry and molecular biology14980-8(2015 May)

MCC inhibits beta-catenin transcriptional activity by sequestering DBC1 in the cytoplasm.
Pangon L, Mladenova D, Watkins L, Van Kralingen C, Currey N, Al-Sohaily S, Lecine P, Borg JP, Kohonen-Corish MR
International journal of cancer136(1)55-64(2015 Jan)

Deleted in breast cancer 1 (DBC1) deficiency results in apoptosis of breast cancer cells through impaired responses to UV-induced DNA damage.
Kim W, Kim JE
Cancer letters333(2)180-6(2013 Jun)

DBC1 (Deleted in Breast Cancer 1) modulates the stability and function of the nuclear receptor Rev-erb?.
Chini CC, Escande C, Nin V, Chini EN
The Biochemical journal451(3)453-61(2013 May)

A high-confidence interaction map identifies SIRT1 as a mediator of acetylation of USP22 and the SAGA coactivator complex.
Armour SM, Bennett EJ, Braun CR, Zhang XY, McMahon SB, Gygi SP, Harper JW, Sinclair DA
Molecular and cellular biology33(8)1487-502(2013 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Ccar2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ccar2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa06256	NM_001170472.1 Latest version!	Rattus norvegicus cell cycle and apoptosis regulator 2 (Ccar2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa06256	XM_006252268.3 Latest version!	Rattus norvegicus cell cycle and apoptosis regulator 2 (Ccar2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa58590	XM_017599702.1 Latest version!	Rattus norvegicus cell cycle and apoptosis regulator 2 (Ccar2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ccar2 ( NM_001170472.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001163943.1 Rattus norvegicus cell cycle and apoptosis regulator 2 (Ccar2), mRNA.

Price & Availability↑

CloneID	ORa06256
Clone ID Related Accession (Same CDS sequence)	XM_006252268.3 , NM_001170472.1
Accession Version	NM_001170472.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2769bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Rattus norvegicus(Norway rat)
Product	cell cycle and apoptosis regulator protein 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from FM034041.1, CK596250.1, CA510912.1, CK359879.1, AC111804.5, EV777431.1, CO557150.1 and AW434320.1. On or before Dec 20, 2009 this sequence version replaced XM_224329.4, XM_001069514.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data from several strains because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and orthologous data. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: FQ211644.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCCAGT TTAAGCGCCA GAGGATCAAC CCACTTCCAG GGGGACGGAA CTTCTCAGGC 
GCAGCTTCAA CATCTCTTTT GGGTCCTCCT CCTGGTCTGC TTACTCCTCC TGTGGCCACA 
GACTTGTCCC AAAATGCCAG GCACCTTCAG AGTGGGGAGA AGCAGCGAGT CTTCACTGGC 
ATTGTTACCA GCTTGCATGA CTACTTTGGG GTGGTAGATG AAGAAGTCTT TTTTCAGCTA 
AGTGTGGTGA AGGGCCGACT ACCCCAGCTG GGTGAGAAGG TGCTGGTGAA GGCTGCGTAT 
AACCCAGGCC AGGCAGTGCC CTGGAATGCT GTCAAGGTGC AGACGCTCTC CAACCAGCCC 
TTACTGAAGT CTCCAGCACC TCCCCTTCTA CATGTGGCAG CTCTGGGCCA GAAGCAAGGG 
ATCCTCGGAG CTCAGCCCCA GTTGATCTTT CAGCCTCACC GAATTCCCCC ACTTTTCCCT 
CAGAAGCCTC TGAGTCTCTT CCAGACATCC CATACACTTC ACCTGAGCCA CCTGAACAGA 
TTTCCTGCTC GGGGTCCTCA TGGAAGGCTG GACCAGGGCC GAAGTGACGA CTATGACTCG 
AAGAAACGCA AACAGCGGGC TGGTGGAGAG CCTTGGGGTG CTAAGAAACC TAGGCATGAC 
CTGCCTCCTT ACCGGGTGCA TCTCACTCCC TATGCTGTGG ACAGTCCCAC CTGTGACTTC 
CTAGAACTGC AGCGACGTTA CCGCAGCCTG TTGGTCCCCT CAGATTTTCT TTCCGTTCAC 
TTGAGCTGGC TGTCAGCCTT CCCACTGGGC CAGCCCTTTT CCCTCCACCA CCCGAGTCGG 
ATCCAGGTCT CTTCAGAGAA GGAGGCAGCT CCAGACACTG GTGCTGAGCC CAGCCCTGAG 
GACAGTGACC CCACTTACAG TTCCAAGGTG CTGCTGCTCT CCTCCCCAGG CCTAGAGGAG 
TTCTATCGTT GCTGCATGCT GTTTGTGGAT GACATGGCTG AGCCAAGGGA GACACCAGAG 
CATCCTCTGA AGCAGCTAAA GTTTTTGCTG GGCCGAAAAG AAGAAGAGGC AGTGCTGGTT 
GGGGGTGAGT GGTCTCCTTC CCTGGATGGC CTCGACCCCC AGGCAGACCC GCAGGTGCTG 
GTGCGCACAG CCATCCGCTG TGCACAAGCC CAGACTGGCA TTGACTTGAG CACCTGTACC 
AAATGGTGGC GTTTTGCTGA GTTTCAGTAT TTGCAGCCGG GGCCGCCCCG GCAGTTACAC 
ACAGTAGTGG TGTACCTGCC AGATGTGTGG ACCATCATGC CCACTTTGGA GGAGTGGGAG 
GCACTGTGCC AACAGAAAGC CACAGAAGCA GCTCCCCAAC CGCAGGAGGC ATCAGGGGAG 
GCAGAGGCTA CTGAACAGGC TCCTGACGTG TCAGAGCAAG CAGACACTTC TAAACAGGAC 
ACAGAGACAG CAGAGGCCAC CACACAGCAA GATGTGGACA CTGAGCTCCC AGAGGCCCCT 
CCGCCTCCTC TAGAACCTGC GGTGATGGCA CGTCCTGGCT GTGTCAACCT GTCTCTCTAC 
GGAATTGTGG AGGATCGGAG GCCAAAAGAA AGGATCTCTT TTGAGGTGGT GGTGCTGGCC 
GAGCTGTTTG TGGAGATGCT GCAGAGGGAC TTTGGCTATA GAATTTATAA GGTGCTGCTG 
AGCCTTCCAG AAAAAGTTGT GTCTCCTCCT GAACCTGAGA AGGAGGAGGC AGCCAAGGAG 
GATGTGGTCA AAGAGGAGGA GGCTGTCAAA GAGGAGGCAG TGAAGGTGTC CAAGGATGAG 
GTACAGAATG AGGGCACAGC TGCCGAGGCA GACAGCCCAC TGAAGGAGGA TGGGCTTCTG 
CCCAAACAGC CCTCTGCAGG GGGAGAAGAG GAGGAGAAGG CCCGGGGTGA GGCAGCTGAG 
GACCTCTGTG AAATGGCCTT GGACCCAGAC CTGCTGCTTC TGAGGGATGA TGGAGAGGAA 
GAGTTTGCAG GAGCAAAGCT AGAGGAAACG GAGGTTCGCT CTGTTGCCTC CAACCAGTCC 
GAGATGGAAT ATTCTTCTCT TCAGGACATG CCTAAGGAGC TGGATCCCTC AGCTGTGCTC 
CCCTTGGACT GCCTTCTGGC TTTTGTGTTT TTTGATGCCA ACTGGTGTGG CTACTTGCAC 
AGGCGAGACT TGGAGAGGAT CCTCCTCACC TTGGGGATCC GGCTCAGTGC AGAGCAGGCC 
AAACAGCTGG TTAGTAGAGT GGTGGCACAG AACATCTGCC AGTATCGGAG CCTTCAGTAT 
AGCCGTGCTG AGGCGCTGGA TGACGGGCTT CCAGAAGACG TGCTCTTTGG AAACCTGGAT 
CTACTGCCTC CTTCAGGAAA GAGCACAAAG CCAGGTGCTG CCCCCACAGA GCACAAAGGC 
CTGGTGCCCC ACAATGGTAG CCTCATCAAT GTGGGAAGCC TGTTACAGCG TGCAGAGCAG 
CAAGACAGTG GCCGCCTGTA CCTGGAGAAC AAGATTCACA CACTGGAATT GAAGCTTGAG 
GAGAGCCATA ACCGATTTTC AGCCACTGAA GTGACAAATA AGACACTGGC AGCAGAGATG 
CAGGAGCTGC GGGCTCGGCT GGCCGAGGCG GAGGAGACAG CCCGGACAGT GGAACGACAG 
AAGAACCAGC TCCAGCGTCA GATGCAGGAC TTCCGCAGAC GCCTGACCCC ACTGCACCTT 
GAGATGCAGC GGATCGTTGA AAAGGCTGAC AGCTGGGTAG AGAAAGAGGA GCCAACGCCT 
AGCAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001163943.1
CDS	110..2878
Translation	MSQFKRQRINPLPGGRNFSGAASTSLLGPPPGLLTPPVATDLSQNARHLQSGEKQRVFTGIVTSLHDYFGVVDEEVFFQLSVVKGRLPQLGEKVLVKAAYNPGQAVPWNAVKVQTLSNQPLLKSPAPPLLHVAALGQKQGILGAQPQLIFQPHRIPPLFPQKPLSLFQTSHTLHLSHLNRFPARGPHGRLDQGRSDDYDSKKRKQRAGGEPWGAKKPRHDLPPYRVHLTPYAVDSPTCDFLELQRRYRSLLVPSDFLSVHLSWLSAFPLGQPFSLHHPSRIQVSSEKEAAPDTGAEPSPEDSDPTYSSKVLLLSSPGLEEFYRCCMLFVDDMAEPRETPEHPLKQLKFLLGRKEEEAVLVGGEWSPSLDGLDPQADPQVLVRTAIRCAQAQTGIDLSTCTKWWRFAEFQYLQPGPPRQLHTVVVYLPDVWTIMPTLEEWEALCQQKATEAAPQPQEASGEAEATEQAPDVSEQADTSKQDTETAEATTQQDVDTELPEAPPPPLEPAVMARPGCVNLSLYGIVEDRRPKERISFEVVVLAELFVEMLQRDFGYRIYKVLLSLPEKVVSPPEPEKEEAAKEDVVKEEEAVKEEAVKVSKDEVQNEGTAAEADSPLKEDGLLPKQPSAGGEEEEKARGEAAEDLCEMALDPDLLLLRDDGEEEFAGAKLEETEVRSVASNQSEMEYSSLQDMPKELDPSAVLPLDCLLAFVFFDANWCGYLHRRDLERILLTLGIRLSAEQAKQLVSRVVAQNICQYRSLQYSRAEALDDGLPEDVLFGNLDLLPPSGKSTKPGAAPTEHKGLVPHNGSLINVGSLLQRAEQQDSGRLYLENKIHTLELKLEESHNRFSATEVTNKTLAAEMQELRARLAEAEETARTVERQKNQLQRQMQDFRRRLTPLHLEMQRIVEKADSWVEKEEPTPSN

Target ORF information:

RefSeq Version	NM_001170472.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus cell cycle and apoptosis regulator 2 (Ccar2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001170472.1

ORF Insert Sequence:

ATGTCCCAGT TTAAGCGCCA GAGGATCAAC CCACTTCCAG GGGGACGGAA CTTCTCAGGC 
GCAGCTTCAA CATCTCTTTT GGGTCCTCCT CCTGGTCTGC TTACTCCTCC TGTGGCCACA 
GACTTGTCCC AAAATGCCAG GCACCTTCAG AGTGGGGAGA AGCAGCGAGT CTTCACTGGC 
ATTGTTACCA GCTTGCATGA CTACTTTGGG GTGGTAGATG AAGAAGTCTT TTTTCAGCTA 
AGTGTGGTGA AGGGCCGACT ACCCCAGCTG GGTGAGAAGG TGCTGGTGAA GGCTGCGTAT 
AACCCAGGCC AGGCAGTGCC CTGGAATGCT GTCAAGGTGC AGACGCTCTC CAACCAGCCC 
TTACTGAAGT CTCCAGCACC TCCCCTTCTA CATGTGGCAG CTCTGGGCCA GAAGCAAGGG 
ATCCTCGGAG CTCAGCCCCA GTTGATCTTT CAGCCTCACC GAATTCCCCC ACTTTTCCCT 
CAGAAGCCTC TGAGTCTCTT CCAGACATCC CATACACTTC ACCTGAGCCA CCTGAACAGA 
TTTCCTGCTC GGGGTCCTCA TGGAAGGCTG GACCAGGGCC GAAGTGACGA CTATGACTCG 
AAGAAACGCA AACAGCGGGC TGGTGGAGAG CCTTGGGGTG CTAAGAAACC TAGGCATGAC 
CTGCCTCCTT ACCGGGTGCA TCTCACTCCC TATGCTGTGG ACAGTCCCAC CTGTGACTTC 
CTAGAACTGC AGCGACGTTA CCGCAGCCTG TTGGTCCCCT CAGATTTTCT TTCCGTTCAC 
TTGAGCTGGC TGTCAGCCTT CCCACTGGGC CAGCCCTTTT CCCTCCACCA CCCGAGTCGG 
ATCCAGGTCT CTTCAGAGAA GGAGGCAGCT CCAGACACTG GTGCTGAGCC CAGCCCTGAG 
GACAGTGACC CCACTTACAG TTCCAAGGTG CTGCTGCTCT CCTCCCCAGG CCTAGAGGAG 
TTCTATCGTT GCTGCATGCT GTTTGTGGAT GACATGGCTG AGCCAAGGGA GACACCAGAG 
CATCCTCTGA AGCAGCTAAA GTTTTTGCTG GGCCGAAAAG AAGAAGAGGC AGTGCTGGTT 
GGGGGTGAGT GGTCTCCTTC CCTGGATGGC CTCGACCCCC AGGCAGACCC GCAGGTGCTG 
GTGCGCACAG CCATCCGCTG TGCACAAGCC CAGACTGGCA TTGACTTGAG CACCTGTACC 
AAATGGTGGC GTTTTGCTGA GTTTCAGTAT TTGCAGCCGG GGCCGCCCCG GCAGTTACAC 
ACAGTAGTGG TGTACCTGCC AGATGTGTGG ACCATCATGC CCACTTTGGA GGAGTGGGAG 
GCACTGTGCC AACAGAAAGC CACAGAAGCA GCTCCCCAAC CGCAGGAGGC ATCAGGGGAG 
GCAGAGGCTA CTGAACAGGC TCCTGACGTG TCAGAGCAAG CAGACACTTC TAAACAGGAC 
ACAGAGACAG CAGAGGCCAC CACACAGCAA GATGTGGACA CTGAGCTCCC AGAGGCCCCT 
CCGCCTCCTC TAGAACCTGC GGTGATGGCA CGTCCTGGCT GTGTCAACCT GTCTCTCTAC 
GGAATTGTGG AGGATCGGAG GCCAAAAGAA AGGATCTCTT TTGAGGTGGT GGTGCTGGCC 
GAGCTGTTTG TGGAGATGCT GCAGAGGGAC TTTGGCTATA GAATTTATAA GGTGCTGCTG 
AGCCTTCCAG AAAAAGTTGT GTCTCCTCCT GAACCTGAGA AGGAGGAGGC AGCCAAGGAG 
GATGTGGTCA AAGAGGAGGA GGCTGTCAAA GAGGAGGCAG TGAAGGTGTC CAAGGATGAG 
GTACAGAATG AGGGCACAGC TGCCGAGGCA GACAGCCCAC TGAAGGAGGA TGGGCTTCTG 
CCCAAACAGC CCTCTGCAGG GGGAGAAGAG GAGGAGAAGG CCCGGGGTGA GGCAGCTGAG 
GACCTCTGTG AAATGGCCTT GGACCCAGAC CTGCTGCTTC TGAGGGATGA TGGAGAGGAA 
GAGTTTGCAG GAGCAAAGCT AGAGGAAACG GAGGTTCGCT CTGTTGCCTC CAACCAGTCC 
GAGATGGAAT ATTCTTCTCT TCAGGACATG CCTAAGGAGC TGGATCCCTC AGCTGTGCTC 
CCCTTGGACT GCCTTCTGGC TTTTGTGTTT TTTGATGCCA ACTGGTGTGG CTACTTGCAC 
AGGCGAGACT TGGAGAGGAT CCTCCTCACC TTGGGGATCC GGCTCAGTGC AGAGCAGGCC 
AAACAGCTGG TTAGTAGAGT GGTGGCACAG AACATCTGCC AGTATCGGAG CCTTCAGTAT 
AGCCGTGCTG AGGCGCTGGA TGACGGGCTT CCAGAAGACG TGCTCTTTGG AAACCTGGAT 
CTACTGCCTC CTTCAGGAAA GAGCACAAAG CCAGGTGCTG CCCCCACAGA GCACAAAGGC 
CTGGTGCCCC ACAATGGTAG CCTCATCAAT GTGGGAAGCC TGTTACAGCG TGCAGAGCAG 
CAAGACAGTG GCCGCCTGTA CCTGGAGAAC AAGATTCACA CACTGGAATT GAAGCTTGAG 
GAGAGCCATA ACCGATTTTC AGCCACTGAA GTGACAAATA AGACACTGGC AGCAGAGATG 
CAGGAGCTGC GGGCTCGGCT GGCCGAGGCG GAGGAGACAG CCCGGACAGT GGAACGACAG 
AAGAACCAGC TCCAGCGTCA GATGCAGGAC TTCCGCAGAC GCCTGACCCC ACTGCACCTT 
GAGATGCAGC GGATCGTTGA AAAGGCTGAC AGCTGGGTAG AGAAAGAGGA GCCAACGCCT 
AGCAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ccar2 ( XM_006252268.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006252330.1 Rattus norvegicus cell cycle and apoptosis regulator 2 (Ccar2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa06256
Clone ID Related Accession (Same CDS sequence)	XM_006252268.3 , NM_001170472.1
Accession Version	XM_006252268.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2769bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	cell cycle and apoptosis regulator protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005114.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006252268.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCCAGT TTAAGCGCCA GAGGATCAAC CCACTTCCAG GGGGACGGAA CTTCTCAGGC 
GCAGCTTCAA CATCTCTTTT GGGTCCTCCT CCTGGTCTGC TTACTCCTCC TGTGGCCACA 
GACTTGTCCC AAAATGCCAG GCACCTTCAG AGTGGGGAGA AGCAGCGAGT CTTCACTGGC 
ATTGTTACCA GCTTGCATGA CTACTTTGGG GTGGTAGATG AAGAAGTCTT TTTTCAGCTA 
AGTGTGGTGA AGGGCCGACT ACCCCAGCTG GGTGAGAAGG TGCTGGTGAA GGCTGCGTAT 
AACCCAGGCC AGGCAGTGCC CTGGAATGCT GTCAAGGTGC AGACGCTCTC CAACCAGCCC 
TTACTGAAGT CTCCAGCACC TCCCCTTCTA CATGTGGCAG CTCTGGGCCA GAAGCAAGGG 
ATCCTCGGAG CTCAGCCCCA GTTGATCTTT CAGCCTCACC GAATTCCCCC ACTTTTCCCT 
CAGAAGCCTC TGAGTCTCTT CCAGACATCC CATACACTTC ACCTGAGCCA CCTGAACAGA 
TTTCCTGCTC GGGGTCCTCA TGGAAGGCTG GACCAGGGCC GAAGTGACGA CTATGACTCG 
AAGAAACGCA AACAGCGGGC TGGTGGAGAG CCTTGGGGTG CTAAGAAACC TAGGCATGAC 
CTGCCTCCTT ACCGGGTGCA TCTCACTCCC TATGCTGTGG ACAGTCCCAC CTGTGACTTC 
CTAGAACTGC AGCGACGTTA CCGCAGCCTG TTGGTCCCCT CAGATTTTCT TTCCGTTCAC 
TTGAGCTGGC TGTCAGCCTT CCCACTGGGC CAGCCCTTTT CCCTCCACCA CCCGAGTCGG 
ATCCAGGTCT CTTCAGAGAA GGAGGCAGCT CCAGACACTG GTGCTGAGCC CAGCCCTGAG 
GACAGTGACC CCACTTACAG TTCCAAGGTG CTGCTGCTCT CCTCCCCAGG CCTAGAGGAG 
TTCTATCGTT GCTGCATGCT GTTTGTGGAT GACATGGCTG AGCCAAGGGA GACACCAGAG 
CATCCTCTGA AGCAGCTAAA GTTTTTGCTG GGCCGAAAAG AAGAAGAGGC AGTGCTGGTT 
GGGGGTGAGT GGTCTCCTTC CCTGGATGGC CTCGACCCCC AGGCAGACCC GCAGGTGCTG 
GTGCGCACAG CCATCCGCTG TGCACAAGCC CAGACTGGCA TTGACTTGAG CACCTGTACC 
AAATGGTGGC GTTTTGCTGA GTTTCAGTAT TTGCAGCCGG GGCCGCCCCG GCAGTTACAC 
ACAGTAGTGG TGTACCTGCC AGATGTGTGG ACCATCATGC CCACTTTGGA GGAGTGGGAG 
GCACTGTGCC AACAGAAAGC CACAGAAGCA GCTCCCCAAC CGCAGGAGGC ATCAGGGGAG 
GCAGAGGCTA CTGAACAGGC TCCTGACGTG TCAGAGCAAG CAGACACTTC TAAACAGGAC 
ACAGAGACAG CAGAGGCCAC CACACAGCAA GATGTGGACA CTGAGCTCCC AGAGGCCCCT 
CCGCCTCCTC TAGAACCTGC GGTGATGGCA CGTCCTGGCT GTGTCAACCT GTCTCTCTAC 
GGAATTGTGG AGGATCGGAG GCCAAAAGAA AGGATCTCTT TTGAGGTGGT GGTGCTGGCC 
GAGCTGTTTG TGGAGATGCT GCAGAGGGAC TTTGGCTATA GAATTTATAA GGTGCTGCTG 
AGCCTTCCAG AAAAAGTTGT GTCTCCTCCT GAACCTGAGA AGGAGGAGGC AGCCAAGGAG 
GATGTGGTCA AAGAGGAGGA GGCTGTCAAA GAGGAGGCAG TGAAGGTGTC CAAGGATGAG 
GTACAGAATG AGGGCACAGC TGCCGAGGCA GACAGCCCAC TGAAGGAGGA TGGGCTTCTG 
CCCAAACAGC CCTCTGCAGG GGGAGAAGAG GAGGAGAAGG CCCGGGGTGA GGCAGCTGAG 
GACCTCTGTG AAATGGCCTT GGACCCAGAC CTGCTGCTTC TGAGGGATGA TGGAGAGGAA 
GAGTTTGCAG GAGCAAAGCT AGAGGAAACG GAGGTTCGCT CTGTTGCCTC CAACCAGTCC 
GAGATGGAAT ATTCTTCTCT TCAGGACATG CCTAAGGAGC TGGATCCCTC AGCTGTGCTC 
CCCTTGGACT GCCTTCTGGC TTTTGTGTTT TTTGATGCCA ACTGGTGTGG CTACTTGCAC 
AGGCGAGACT TGGAGAGGAT CCTCCTCACC TTGGGGATCC GGCTCAGTGC AGAGCAGGCC 
AAACAGCTGG TTAGTAGAGT GGTGGCACAG AACATCTGCC AGTATCGGAG CCTTCAGTAT 
AGCCGTGCTG AGGCGCTGGA TGACGGGCTT CCAGAAGACG TGCTCTTTGG AAACCTGGAT 
CTACTGCCTC CTTCAGGAAA GAGCACAAAG CCAGGTGCTG CCCCCACAGA GCACAAAGGC 
CTGGTGCCCC ACAATGGTAG CCTCATCAAT GTGGGAAGCC TGTTACAGCG TGCAGAGCAG 
CAAGACAGTG GCCGCCTGTA CCTGGAGAAC AAGATTCACA CACTGGAATT GAAGCTTGAG 
GAGAGCCATA ACCGATTTTC AGCCACTGAA GTGACAAATA AGACACTGGC AGCAGAGATG 
CAGGAGCTGC GGGCTCGGCT GGCCGAGGCG GAGGAGACAG CCCGGACAGT GGAACGACAG 
AAGAACCAGC TCCAGCGTCA GATGCAGGAC TTCCGCAGAC GCCTGACCCC ACTGCACCTT 
GAGATGCAGC GGATCGTTGA AAAGGCTGAC AGCTGGGTAG AGAAAGAGGA GCCAACGCCT 
AGCAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006252330.1
CDS	129..2897
Translation	MSQFKRQRINPLPGGRNFSGAASTSLLGPPPGLLTPPVATDLSQNARHLQSGEKQRVFTGIVTSLHDYFGVVDEEVFFQLSVVKGRLPQLGEKVLVKAAYNPGQAVPWNAVKVQTLSNQPLLKSPAPPLLHVAALGQKQGILGAQPQLIFQPHRIPPLFPQKPLSLFQTSHTLHLSHLNRFPARGPHGRLDQGRSDDYDSKKRKQRAGGEPWGAKKPRHDLPPYRVHLTPYAVDSPTCDFLELQRRYRSLLVPSDFLSVHLSWLSAFPLGQPFSLHHPSRIQVSSEKEAAPDTGAEPSPEDSDPTYSSKVLLLSSPGLEEFYRCCMLFVDDMAEPRETPEHPLKQLKFLLGRKEEEAVLVGGEWSPSLDGLDPQADPQVLVRTAIRCAQAQTGIDLSTCTKWWRFAEFQYLQPGPPRQLHTVVVYLPDVWTIMPTLEEWEALCQQKATEAAPQPQEASGEAEATEQAPDVSEQADTSKQDTETAEATTQQDVDTELPEAPPPPLEPAVMARPGCVNLSLYGIVEDRRPKERISFEVVVLAELFVEMLQRDFGYRIYKVLLSLPEKVVSPPEPEKEEAAKEDVVKEEEAVKEEAVKVSKDEVQNEGTAAEADSPLKEDGLLPKQPSAGGEEEEKARGEAAEDLCEMALDPDLLLLRDDGEEEFAGAKLEETEVRSVASNQSEMEYSSLQDMPKELDPSAVLPLDCLLAFVFFDANWCGYLHRRDLERILLTLGIRLSAEQAKQLVSRVVAQNICQYRSLQYSRAEALDDGLPEDVLFGNLDLLPPSGKSTKPGAAPTEHKGLVPHNGSLINVGSLLQRAEQQDSGRLYLENKIHTLELKLEESHNRFSATEVTNKTLAAEMQELRARLAEAEETARTVERQKNQLQRQMQDFRRRLTPLHLEMQRIVEKADSWVEKEEPTPSN

Target ORF information:

RefSeq Version	XM_006252268.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus cell cycle and apoptosis regulator 2 (Ccar2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006252268.3

ORF Insert Sequence:

ATGTCCCAGT TTAAGCGCCA GAGGATCAAC CCACTTCCAG GGGGACGGAA CTTCTCAGGC 
GCAGCTTCAA CATCTCTTTT GGGTCCTCCT CCTGGTCTGC TTACTCCTCC TGTGGCCACA 
GACTTGTCCC AAAATGCCAG GCACCTTCAG AGTGGGGAGA AGCAGCGAGT CTTCACTGGC 
ATTGTTACCA GCTTGCATGA CTACTTTGGG GTGGTAGATG AAGAAGTCTT TTTTCAGCTA 
AGTGTGGTGA AGGGCCGACT ACCCCAGCTG GGTGAGAAGG TGCTGGTGAA GGCTGCGTAT 
AACCCAGGCC AGGCAGTGCC CTGGAATGCT GTCAAGGTGC AGACGCTCTC CAACCAGCCC 
TTACTGAAGT CTCCAGCACC TCCCCTTCTA CATGTGGCAG CTCTGGGCCA GAAGCAAGGG 
ATCCTCGGAG CTCAGCCCCA GTTGATCTTT CAGCCTCACC GAATTCCCCC ACTTTTCCCT 
CAGAAGCCTC TGAGTCTCTT CCAGACATCC CATACACTTC ACCTGAGCCA CCTGAACAGA 
TTTCCTGCTC GGGGTCCTCA TGGAAGGCTG GACCAGGGCC GAAGTGACGA CTATGACTCG 
AAGAAACGCA AACAGCGGGC TGGTGGAGAG CCTTGGGGTG CTAAGAAACC TAGGCATGAC 
CTGCCTCCTT ACCGGGTGCA TCTCACTCCC TATGCTGTGG ACAGTCCCAC CTGTGACTTC 
CTAGAACTGC AGCGACGTTA CCGCAGCCTG TTGGTCCCCT CAGATTTTCT TTCCGTTCAC 
TTGAGCTGGC TGTCAGCCTT CCCACTGGGC CAGCCCTTTT CCCTCCACCA CCCGAGTCGG 
ATCCAGGTCT CTTCAGAGAA GGAGGCAGCT CCAGACACTG GTGCTGAGCC CAGCCCTGAG 
GACAGTGACC CCACTTACAG TTCCAAGGTG CTGCTGCTCT CCTCCCCAGG CCTAGAGGAG 
TTCTATCGTT GCTGCATGCT GTTTGTGGAT GACATGGCTG AGCCAAGGGA GACACCAGAG 
CATCCTCTGA AGCAGCTAAA GTTTTTGCTG GGCCGAAAAG AAGAAGAGGC AGTGCTGGTT 
GGGGGTGAGT GGTCTCCTTC CCTGGATGGC CTCGACCCCC AGGCAGACCC GCAGGTGCTG 
GTGCGCACAG CCATCCGCTG TGCACAAGCC CAGACTGGCA TTGACTTGAG CACCTGTACC 
AAATGGTGGC GTTTTGCTGA GTTTCAGTAT TTGCAGCCGG GGCCGCCCCG GCAGTTACAC 
ACAGTAGTGG TGTACCTGCC AGATGTGTGG ACCATCATGC CCACTTTGGA GGAGTGGGAG 
GCACTGTGCC AACAGAAAGC CACAGAAGCA GCTCCCCAAC CGCAGGAGGC ATCAGGGGAG 
GCAGAGGCTA CTGAACAGGC TCCTGACGTG TCAGAGCAAG CAGACACTTC TAAACAGGAC 
ACAGAGACAG CAGAGGCCAC CACACAGCAA GATGTGGACA CTGAGCTCCC AGAGGCCCCT 
CCGCCTCCTC TAGAACCTGC GGTGATGGCA CGTCCTGGCT GTGTCAACCT GTCTCTCTAC 
GGAATTGTGG AGGATCGGAG GCCAAAAGAA AGGATCTCTT TTGAGGTGGT GGTGCTGGCC 
GAGCTGTTTG TGGAGATGCT GCAGAGGGAC TTTGGCTATA GAATTTATAA GGTGCTGCTG 
AGCCTTCCAG AAAAAGTTGT GTCTCCTCCT GAACCTGAGA AGGAGGAGGC AGCCAAGGAG 
GATGTGGTCA AAGAGGAGGA GGCTGTCAAA GAGGAGGCAG TGAAGGTGTC CAAGGATGAG 
GTACAGAATG AGGGCACAGC TGCCGAGGCA GACAGCCCAC TGAAGGAGGA TGGGCTTCTG 
CCCAAACAGC CCTCTGCAGG GGGAGAAGAG GAGGAGAAGG CCCGGGGTGA GGCAGCTGAG 
GACCTCTGTG AAATGGCCTT GGACCCAGAC CTGCTGCTTC TGAGGGATGA TGGAGAGGAA 
GAGTTTGCAG GAGCAAAGCT AGAGGAAACG GAGGTTCGCT CTGTTGCCTC CAACCAGTCC 
GAGATGGAAT ATTCTTCTCT TCAGGACATG CCTAAGGAGC TGGATCCCTC AGCTGTGCTC 
CCCTTGGACT GCCTTCTGGC TTTTGTGTTT TTTGATGCCA ACTGGTGTGG CTACTTGCAC 
AGGCGAGACT TGGAGAGGAT CCTCCTCACC TTGGGGATCC GGCTCAGTGC AGAGCAGGCC 
AAACAGCTGG TTAGTAGAGT GGTGGCACAG AACATCTGCC AGTATCGGAG CCTTCAGTAT 
AGCCGTGCTG AGGCGCTGGA TGACGGGCTT CCAGAAGACG TGCTCTTTGG AAACCTGGAT 
CTACTGCCTC CTTCAGGAAA GAGCACAAAG CCAGGTGCTG CCCCCACAGA GCACAAAGGC 
CTGGTGCCCC ACAATGGTAG CCTCATCAAT GTGGGAAGCC TGTTACAGCG TGCAGAGCAG 
CAAGACAGTG GCCGCCTGTA CCTGGAGAAC AAGATTCACA CACTGGAATT GAAGCTTGAG 
GAGAGCCATA ACCGATTTTC AGCCACTGAA GTGACAAATA AGACACTGGC AGCAGAGATG 
CAGGAGCTGC GGGCTCGGCT GGCCGAGGCG GAGGAGACAG CCCGGACAGT GGAACGACAG 
AAGAACCAGC TCCAGCGTCA GATGCAGGAC TTCCGCAGAC GCCTGACCCC ACTGCACCTT 
GAGATGCAGC GGATCGTTGA AAAGGCTGAC AGCTGGGTAG AGAAAGAGGA GCCAACGCCT 
AGCAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ccar2 ( XM_017599702.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017455191.1 Rattus norvegicus cell cycle and apoptosis regulator 2 (Ccar2), transcript variant X2, mRNA.

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CloneID	ORa58590
Clone ID Related Accession (Same CDS sequence)	XM_017599702.1
Accession Version	XM_017599702.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2766bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	cell cycle and apoptosis regulator protein 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005114.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCCAGT TTAAGCGCCA GAGGATCAAC CCACTTCCAG GGGGACGGAA CTTCTCAGGC 
GCAGCTTCAA CATCTCTTTT GGGTCCTCCT CCTGGTCTGC TTACTCCTCC TGTGGCCACA 
GACTTGTCCC AAAATGCCAG GCACCTTCAG AGTGGGGAGA AGCAGCGAGT CTTCACTGGC 
ATTGTTACCA GCTTGCATGA CTACTTTGGG GTGGTAGATG AAGAAGTCTT TTTTCAGCTA 
AGTGTGGTGA AGGGCCGACT ACCCCAGCTG GGTGAGAAGG TGCTGGTGAA GGCTGCGTAT 
AACCCAGGCC AGGCAGTGCC CTGGAATGCT GTCAAGGTGC AGACGCTCTC CAACCAGCCC 
TTACTGAAGT CTCCAGCACC TCCCCTTCTA CATGTGGCAG CTCTGGGCCA GAAGCAAGGG 
ATCCTCGGAG CTCAGCCCCA GTTGATCTTT CAGCCTCACC GAATTCCCCC ACTTTTCCCT 
CAGAAGCCTC TGAGTCTCTT CCAGACATCC CATACACTTC ACCTGAGCCA CCTGAACAGA 
TTTCCTGCTC GGGGTCCTCA TGGAAGGCTG GACCAGGGCC GAAGTGACGA CTATGACTCG 
AAGAAACGCA AACAGCGGGC TGGTGGAGAG CCTTGGGGTG CTAAGAAACC TAGGCATGAC 
CTGCCTCCTT ACCGGGTGCA TCTCACTCCC TATGCTGTGG ACAGTCCCAC CTGTGACTTC 
CTAGAACTGC AGCGACGTTA CCGCAGCCTG TTGGTCCCCT CAGATTTTCT TTCCGTTCAC 
TTGAGCTGGC TGTCAGCCTT CCCACTGGGC CAGCCCTTTT CCCTCCACCA CCCGAGTCGG 
ATCCAGGTCT CTTCAGAGAA GGAGGCAGCT CCAGACACTG GTGCTGAGCC CAGCCCTGAG 
GACAGTGACC CCACTTACAG TTCCAAGGTG CTGCTGCTCT CCTCCCCAGG CCTAGAGGAG 
TTCTATCGTT GCTGCATGCT GTTTGTGGAT GACATGGCTG AGCCAAGGGA GACACCAGAG 
CATCCTCTGA AGCAGCTAAA GTTTTTGCTG GGCCGAAAAG AAGAAGAGGC AGTGCTGGTT 
GGGGGTGAGT GGTCTCCTTC CCTGGATGGC CTCGACCCCC AGGCAGACCC GCAGGTGCTG 
GTGCGCACAG CCATCCGCTG TGCACAAGCC CAGACTGGCA TTGACTTGAG CACCTGTACC 
AAATGGTGGC GTTTTGCTGA GTTTCAGTAT TTGCAGCCGG GGCCGCCCCG GCAGTTACAC 
ACAGTAGTGG TGTACCTGCC AGATGTGTGG ACCATCATGC CCACTTTGGA GGAGTGGGAG 
GCACTGTGCC AACAGAAAGC CACAGAAGCA GCTCCCCAAC CGCAGGAGGC ATCAGGGGAG 
GCAGAGGCTA CTGAACAGGC TCCTGACGTG TCAGAGCAAG CAGACACTTC TAAACAGGAC 
ACAGAGACAG CAGAGGCCAC CACACAGCAA GATGTGGACA CTGAGCTCCC AGAGGCCCCT 
CCGCCTCCTC TAGAACCTGC GGTGATGGCA CGTCCTGGCT GTGTCAACCT GTCTCTCTAC 
GGAATTGTGG AGGATCGGAG GCCAAAAGAA AGGATCTCTT TTGAGGTGGT GGTGCTGGCC 
GAGCTGTTTG TGGAGATGCT GCAGAGGGAC TTTGGCTATA GAATTTATAA GGTGCTGCTG 
AGCCTTCCAG AAAAAGTTGT GTCTCCTCCT GAACCTGAGA AGGAGGAGGC AGCCAAGGAG 
GATGTGGTCA AAGAGGAGGA GGCTGTCAAA GAGGAGGCAG TGAAGGTGTC CAAGGATGAG 
GTACAGAATG AGGGCACAGC TGCCGAGGCA GACAGCCCAC TGAAGGAGGA TGGGCTTCTG 
CCCAAACAGC CCTCTGCAGG GGGAGAAGAG GAGGAGAAGG CCCGGGGTGA GGCAGCTGAG 
GACCTCTGTG AAATGGCCTT GGACCCAGAC CTGCTGCTTC TGAGGGATGA TGGAGAGGAA 
GAGTTTGGAG CAAAGCTAGA GGAAACGGAG GTTCGCTCTG TTGCCTCCAA CCAGTCCGAG 
ATGGAATATT CTTCTCTTCA GGACATGCCT AAGGAGCTGG ATCCCTCAGC TGTGCTCCCC 
TTGGACTGCC TTCTGGCTTT TGTGTTTTTT GATGCCAACT GGTGTGGCTA CTTGCACAGG 
CGAGACTTGG AGAGGATCCT CCTCACCTTG GGGATCCGGC TCAGTGCAGA GCAGGCCAAA 
CAGCTGGTTA GTAGAGTGGT GGCACAGAAC ATCTGCCAGT ATCGGAGCCT TCAGTATAGC 
CGTGCTGAGG CGCTGGATGA CGGGCTTCCA GAAGACGTGC TCTTTGGAAA CCTGGATCTA 
CTGCCTCCTT CAGGAAAGAG CACAAAGCCA GGTGCTGCCC CCACAGAGCA CAAAGGCCTG 
GTGCCCCACA ATGGTAGCCT CATCAATGTG GGAAGCCTGT TACAGCGTGC AGAGCAGCAA 
GACAGTGGCC GCCTGTACCT GGAGAACAAG ATTCACACAC TGGAATTGAA GCTTGAGGAG 
AGCCATAACC GATTTTCAGC CACTGAAGTG ACAAATAAGA CACTGGCAGC AGAGATGCAG 
GAGCTGCGGG CTCGGCTGGC CGAGGCGGAG GAGACAGCCC GGACAGTGGA ACGACAGAAG 
AACCAGCTCC AGCGTCAGAT GCAGGACTTC CGCAGACGCC TGACCCCACT GCACCTTGAG 
ATGCAGCGGA TCGTTGAAAA GGCTGACAGC TGGGTAGAGA AAGAGGAGCC AACGCCTAGC 
AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017455191.1
CDS	523..3288
Translation	MSQFKRQRINPLPGGRNFSGAASTSLLGPPPGLLTPPVATDLSQNARHLQSGEKQRVFTGIVTSLHDYFGVVDEEVFFQLSVVKGRLPQLGEKVLVKAAYNPGQAVPWNAVKVQTLSNQPLLKSPAPPLLHVAALGQKQGILGAQPQLIFQPHRIPPLFPQKPLSLFQTSHTLHLSHLNRFPARGPHGRLDQGRSDDYDSKKRKQRAGGEPWGAKKPRHDLPPYRVHLTPYAVDSPTCDFLELQRRYRSLLVPSDFLSVHLSWLSAFPLGQPFSLHHPSRIQVSSEKEAAPDTGAEPSPEDSDPTYSSKVLLLSSPGLEEFYRCCMLFVDDMAEPRETPEHPLKQLKFLLGRKEEEAVLVGGEWSPSLDGLDPQADPQVLVRTAIRCAQAQTGIDLSTCTKWWRFAEFQYLQPGPPRQLHTVVVYLPDVWTIMPTLEEWEALCQQKATEAAPQPQEASGEAEATEQAPDVSEQADTSKQDTETAEATTQQDVDTELPEAPPPPLEPAVMARPGCVNLSLYGIVEDRRPKERISFEVVVLAELFVEMLQRDFGYRIYKVLLSLPEKVVSPPEPEKEEAAKEDVVKEEEAVKEEAVKVSKDEVQNEGTAAEADSPLKEDGLLPKQPSAGGEEEEKARGEAAEDLCEMALDPDLLLLRDDGEEEFGAKLEETEVRSVASNQSEMEYSSLQDMPKELDPSAVLPLDCLLAFVFFDANWCGYLHRRDLERILLTLGIRLSAEQAKQLVSRVVAQNICQYRSLQYSRAEALDDGLPEDVLFGNLDLLPPSGKSTKPGAAPTEHKGLVPHNGSLINVGSLLQRAEQQDSGRLYLENKIHTLELKLEESHNRFSATEVTNKTLAAEMQELRARLAEAEETARTVERQKNQLQRQMQDFRRRLTPLHLEMQRIVEKADSWVEKEEPTPSN

Target ORF information:

RefSeq Version	XM_017599702.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus cell cycle and apoptosis regulator 2 (Ccar2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017599702.1

ORF Insert Sequence:

ATGTCCCAGT TTAAGCGCCA GAGGATCAAC CCACTTCCAG GGGGACGGAA CTTCTCAGGC 
GCAGCTTCAA CATCTCTTTT GGGTCCTCCT CCTGGTCTGC TTACTCCTCC TGTGGCCACA 
GACTTGTCCC AAAATGCCAG GCACCTTCAG AGTGGGGAGA AGCAGCGAGT CTTCACTGGC 
ATTGTTACCA GCTTGCATGA CTACTTTGGG GTGGTAGATG AAGAAGTCTT TTTTCAGCTA 
AGTGTGGTGA AGGGCCGACT ACCCCAGCTG GGTGAGAAGG TGCTGGTGAA GGCTGCGTAT 
AACCCAGGCC AGGCAGTGCC CTGGAATGCT GTCAAGGTGC AGACGCTCTC CAACCAGCCC 
TTACTGAAGT CTCCAGCACC TCCCCTTCTA CATGTGGCAG CTCTGGGCCA GAAGCAAGGG 
ATCCTCGGAG CTCAGCCCCA GTTGATCTTT CAGCCTCACC GAATTCCCCC ACTTTTCCCT 
CAGAAGCCTC TGAGTCTCTT CCAGACATCC CATACACTTC ACCTGAGCCA CCTGAACAGA 
TTTCCTGCTC GGGGTCCTCA TGGAAGGCTG GACCAGGGCC GAAGTGACGA CTATGACTCG 
AAGAAACGCA AACAGCGGGC TGGTGGAGAG CCTTGGGGTG CTAAGAAACC TAGGCATGAC 
CTGCCTCCTT ACCGGGTGCA TCTCACTCCC TATGCTGTGG ACAGTCCCAC CTGTGACTTC 
CTAGAACTGC AGCGACGTTA CCGCAGCCTG TTGGTCCCCT CAGATTTTCT TTCCGTTCAC 
TTGAGCTGGC TGTCAGCCTT CCCACTGGGC CAGCCCTTTT CCCTCCACCA CCCGAGTCGG 
ATCCAGGTCT CTTCAGAGAA GGAGGCAGCT CCAGACACTG GTGCTGAGCC CAGCCCTGAG 
GACAGTGACC CCACTTACAG TTCCAAGGTG CTGCTGCTCT CCTCCCCAGG CCTAGAGGAG 
TTCTATCGTT GCTGCATGCT GTTTGTGGAT GACATGGCTG AGCCAAGGGA GACACCAGAG 
CATCCTCTGA AGCAGCTAAA GTTTTTGCTG GGCCGAAAAG AAGAAGAGGC AGTGCTGGTT 
GGGGGTGAGT GGTCTCCTTC CCTGGATGGC CTCGACCCCC AGGCAGACCC GCAGGTGCTG 
GTGCGCACAG CCATCCGCTG TGCACAAGCC CAGACTGGCA TTGACTTGAG CACCTGTACC 
AAATGGTGGC GTTTTGCTGA GTTTCAGTAT TTGCAGCCGG GGCCGCCCCG GCAGTTACAC 
ACAGTAGTGG TGTACCTGCC AGATGTGTGG ACCATCATGC CCACTTTGGA GGAGTGGGAG 
GCACTGTGCC AACAGAAAGC CACAGAAGCA GCTCCCCAAC CGCAGGAGGC ATCAGGGGAG 
GCAGAGGCTA CTGAACAGGC TCCTGACGTG TCAGAGCAAG CAGACACTTC TAAACAGGAC 
ACAGAGACAG CAGAGGCCAC CACACAGCAA GATGTGGACA CTGAGCTCCC AGAGGCCCCT 
CCGCCTCCTC TAGAACCTGC GGTGATGGCA CGTCCTGGCT GTGTCAACCT GTCTCTCTAC 
GGAATTGTGG AGGATCGGAG GCCAAAAGAA AGGATCTCTT TTGAGGTGGT GGTGCTGGCC 
GAGCTGTTTG TGGAGATGCT GCAGAGGGAC TTTGGCTATA GAATTTATAA GGTGCTGCTG 
AGCCTTCCAG AAAAAGTTGT GTCTCCTCCT GAACCTGAGA AGGAGGAGGC AGCCAAGGAG 
GATGTGGTCA AAGAGGAGGA GGCTGTCAAA GAGGAGGCAG TGAAGGTGTC CAAGGATGAG 
GTACAGAATG AGGGCACAGC TGCCGAGGCA GACAGCCCAC TGAAGGAGGA TGGGCTTCTG 
CCCAAACAGC CCTCTGCAGG GGGAGAAGAG GAGGAGAAGG CCCGGGGTGA GGCAGCTGAG 
GACCTCTGTG AAATGGCCTT GGACCCAGAC CTGCTGCTTC TGAGGGATGA TGGAGAGGAA 
GAGTTTGGAG CAAAGCTAGA GGAAACGGAG GTTCGCTCTG TTGCCTCCAA CCAGTCCGAG 
ATGGAATATT CTTCTCTTCA GGACATGCCT AAGGAGCTGG ATCCCTCAGC TGTGCTCCCC 
TTGGACTGCC TTCTGGCTTT TGTGTTTTTT GATGCCAACT GGTGTGGCTA CTTGCACAGG 
CGAGACTTGG AGAGGATCCT CCTCACCTTG GGGATCCGGC TCAGTGCAGA GCAGGCCAAA 
CAGCTGGTTA GTAGAGTGGT GGCACAGAAC ATCTGCCAGT ATCGGAGCCT TCAGTATAGC 
CGTGCTGAGG CGCTGGATGA CGGGCTTCCA GAAGACGTGC TCTTTGGAAA CCTGGATCTA 
CTGCCTCCTT CAGGAAAGAG CACAAAGCCA GGTGCTGCCC CCACAGAGCA CAAAGGCCTG 
GTGCCCCACA ATGGTAGCCT CATCAATGTG GGAAGCCTGT TACAGCGTGC AGAGCAGCAA 
GACAGTGGCC GCCTGTACCT GGAGAACAAG ATTCACACAC TGGAATTGAA GCTTGAGGAG 
AGCCATAACC GATTTTCAGC CACTGAAGTG ACAAATAAGA CACTGGCAGC AGAGATGCAG 
GAGCTGCGGG CTCGGCTGGC CGAGGCGGAG GAGACAGCCC GGACAGTGGA ACGACAGAAG 
AACCAGCTCC AGCGTCAGAT GCAGGACTTC CGCAGACGCC TGACCCCACT GCACCTTGAG 
ATGCAGCGGA TCGTTGAAAA GGCTGACAGC TGGGTAGAGA AAGAGGAGCC AACGCCTAGC 
AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

CCAR2 negatively regulates nuclear receptor LXR? by competing with SIRT1 deacetylase.
The Journal of steroid biochemistry and molecular biology14980-8(2015 May)
Sakurabashi A,Wada-Hiraike O,Hirano M,Fu H,Isono W,Fukuda T,Morita Y,Tanikawa M,Miyamoto Y,Oda K,Kawana K,Osuga Y,Fujii T

MCC inhibits beta-catenin transcriptional activity by sequestering DBC1 in the cytoplasm.
International journal of cancer136(1)55-64(2015 Jan)
Pangon L,Mladenova D,Watkins L,Van Kralingen C,Currey N,Al-Sohaily S,Lecine P,Borg JP,Kohonen-Corish MR

Deleted in breast cancer 1 (DBC1) deficiency results in apoptosis of breast cancer cells through impaired responses to UV-induced DNA damage.
Cancer letters333(2)180-6(2013 Jun)
Kim W,Kim JE

DBC1 (Deleted in Breast Cancer 1) modulates the stability and function of the nuclear receptor Rev-erb?.
The Biochemical journal451(3)453-61(2013 May)
Chini CC,Escande C,Nin V,Chini EN

A high-confidence interaction map identifies SIRT1 as a mediator of acetylation of USP22 and the SAGA coactivator complex.
Molecular and cellular biology33(8)1487-502(2013 Apr)
Armour SM,Bennett EJ,Braun CR,Zhang XY,McMahon SB,Gygi SP,Harper JW,Sinclair DA