Med28 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Med28
Entrez Gene ID	305391
Full Name	mediator complex subunit 28
Synonyms	Eg1,FKSG20,RGD1305875
General protein information	Preferred Names mediator complex subunit 28 Names mediator of RNA polymerase II transcription subunit 28 endothelial-derived mediator of RNA polymerase II transcription, subunit 28 homolog
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 14 Map Location: 14q11

mRNA and Protein(s)

mRNA	Protein	Name
NM_001107217.1	NP_001100687.1	mediator of RNA polymerase II transcription subunit 28

Pathways from BioSystems

Homologies

Bos taurus (cattle)	MED28	NP_001033627.1
Drosophila melanogaster (fruit fly)	MED28	NP_651397.1
Xenopus tropicalis (tropical clawed frog)	med28	NP_001096476.1
	LOC722863	XP_001118952.2
Mus musculus (house mouse)	Med28	NP_080171.1
Homo sapiens (human)	MED28	NP_079481.2
Canis lupus familiaris (dog)	MED28	XP_850777.1
Rattus norvegicus (Norway rat)	Med28	NP_001100687.1
Gallus gallus (chicken)	MED28	XP_003641296.2
Danio rerio (zebrafish)	med28	NP_001007771.1
Pan troglodytes (chimpanzee)	MED28	XP_003950342.1

Gene Ontology
Bibliography

Related articles in PubMed

The Mediator complex and transcription regulation.
Poss ZC, Ebmeier CC, Taatjes DJ
Critical reviews in biochemistry and molecular biology48(6)575-608(2013 Nov-Dec)

Mediator and cohesin connect gene expression and chromatin architecture.
Kagey MH, Newman JJ, Bilodeau S, Zhan Y, Orlando DA, van Berkum NL, Ebmeier CC, Goossens J, Rahl PB, Levine SS, Taatjes DJ, Dekker J, Young RA
Nature467(7314)430-5(2010 Sep)

ATAC and Mediator coactivators form a stable complex and regulate a set of non-coding RNA genes.
Krebs AR, Demmers J, Karmodiya K, Chang NC, Chang AC, Tora L
EMBO reports11(7)541-7(2010 Jul)

Mediator subunit MED28 (Magicin) is a repressor of smooth muscle cell differentiation.
Beyer KS, Beauchamp RL, Lee MF, Gusella JF, N??r AM, Ramesh V
The Journal of biological chemistry282(44)32152-7(2007 Nov)

Magicin, a novel cytoskeletal protein associates with the NF2 tumor suppressor merlin and Grb2.
Wiederhold T, Lee MF, James M, Neujahr R, Smith N, Murthy A, Hartwig J, Gusella JF, Ramesh V
Oncogene23(54)8815-25(2004 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Med28 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Med28 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa05244	NM_001107217.1 Latest version!	Rattus norvegicus mediator complex subunit 28 (Med28), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Med28 ( NM_001107217.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001100687.1 Rattus norvegicus mediator complex subunit 28 (Med28), mRNA.

Price & Availability↑

CloneID	ORa05244
Clone ID Related Accession (Same CDS sequence)	NM_001107217.1
Accession Version	NM_001107217.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-09-15
Organism	Rattus norvegicus(Norway rat)
Product	mediator of RNA polymerase II transcription subunit 28
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473963.1. On or before Oct 4, 2007 this sequence version replaced XM_001059017.1, XM_223463.4. ##Evidence-Data-START## Transcript exon combination :: FQ218099.1, FQ211206.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGCGT CCCTGGGAGG GATGTTCGCC GGCCAGCCTC CAGGACCTCC TCCGCCACCG 
CCCGGGCTCC CAGGCCAGGC GTCGCTTCTA CAAGCAGCCC CTGGGGCTCC GAGACCATCT 
AACAGCACTT TGGTGGACGA GTTGGAATCA TCATTCGAGG CTTGCTTTGC TTCCCTTGTG 
AGTCAGGATT ATGTCAATGG GACTGATCAG GAAGAAATTC GAACTGGTGT TGATCAGTGT 
ATCCAGAAGT TTTTGGACAT TGCAAGACAG ACAGAATGCT TTTTCTTACA AAAAAGGTTG 
CAATTGTCTG TCCAGAAACC AGATCAAGTT ATCAAAGAGG ATGTCTCAGA ACTAAGGAGT 
GAATTGCAGC GGAAAGATGC TCTGGTCCAG AAGCACTTGA CAAAACTGAG ACATTGGCAG 
CAGGTGCTGG AAGACATCAA TGTGCAGCAC AAGAAGCCAG CCGACATGCC TCAGGGCTCC 
TTGGCCTACC TTGAGCAGGC ATCTGCCAAC ATACCTGCAC CTCTGAAGCA GACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001100687.1
CDS	43..579
Translation	MAASLGGMFAGQPPGPPPPPPGLPGQASLLQAAPGAPRPSNSTLVDELESSFEACFASLVSQDYVNGTDQEEIRTGVDQCIQKFLDIARQTECFFLQKRLQLSVQKPDQVIKEDVSELRSELQRKDALVQKHLTKLRHWQQVLEDINVQHKKPADMPQGSLAYLEQASANIPAPLKQT

Target ORF information:

RefSeq Version	NM_001107217.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus mediator complex subunit 28 (Med28), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001107217.1

ORF Insert Sequence:

ATGGCGGCGT CCCTGGGAGG GATGTTCGCC GGCCAGCCTC CAGGACCTCC TCCGCCACCG 
CCCGGGCTCC CAGGCCAGGC GTCGCTTCTA CAAGCAGCCC CTGGGGCTCC GAGACCATCT 
AACAGCACTT TGGTGGACGA GTTGGAATCA TCATTCGAGG CTTGCTTTGC TTCCCTTGTG 
AGTCAGGATT ATGTCAATGG GACTGATCAG GAAGAAATTC GAACTGGTGT TGATCAGTGT 
ATCCAGAAGT TTTTGGACAT TGCAAGACAG ACAGAATGCT TTTTCTTACA AAAAAGGTTG 
CAATTGTCTG TCCAGAAACC AGATCAAGTT ATCAAAGAGG ATGTCTCAGA ACTAAGGAGT 
GAATTGCAGC GGAAAGATGC TCTGGTCCAG AAGCACTTGA CAAAACTGAG ACATTGGCAG 
CAGGTGCTGG AAGACATCAA TGTGCAGCAC AAGAAGCCAG CCGACATGCC TCAGGGCTCC 
TTGGCCTACC TTGAGCAGGC ATCTGCCAAC ATACCTGCAC CTCTGAAGCA GACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The Mediator complex and transcription regulation.
Critical reviews in biochemistry and molecular biology48(6)575-608(2013 Nov-Dec)
Poss ZC,Ebmeier CC,Taatjes DJ

Mediator and cohesin connect gene expression and chromatin architecture.
Nature467(7314)430-5(2010 Sep)
Kagey MH,Newman JJ,Bilodeau S,Zhan Y,Orlando DA,van Berkum NL,Ebmeier CC,Goossens J,Rahl PB,Levine SS,Taatjes DJ,Dekker J,Young RA

ATAC and Mediator coactivators form a stable complex and regulate a set of non-coding RNA genes.
EMBO reports11(7)541-7(2010 Jul)
Krebs AR,Demmers J,Karmodiya K,Chang NC,Chang AC,Tora L

Mediator subunit MED28 (Magicin) is a repressor of smooth muscle cell differentiation.
The Journal of biological chemistry282(44)32152-7(2007 Nov)
Beyer KS,Beauchamp RL,Lee MF,Gusella JF,N??r AM,Ramesh V

Magicin, a novel cytoskeletal protein associates with the NF2 tumor suppressor merlin and Grb2.
Oncogene23(54)8815-25(2004 Nov)
Wiederhold T,Lee MF,James M,Neujahr R,Smith N,Murthy A,Hartwig J,Gusella JF,Ramesh V