Papolb cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Papolb
Entrez Gene ID	304300
Full Name	poly(A) polymerase beta
General protein information	Preferred Names poly(A) polymerase beta Names poly(A) polymerase beta poly (A) polymerase beta (testis specific)
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 12 Map Location: 12p11

mRNA and Protein(s)

mRNA	Protein	Name
NM_001012020.2	NP_001012020.1	poly(A) polymerase beta

Pathways from BioSystems

Homologies

Homo sapiens (human)	PAPOLB	NP_064529.4
	PAPOLB	XP_002803238.1
Mus musculus (house mouse)	Papolb	NP_064327.1
Rattus norvegicus (Norway rat)	Papolb	NP_001012020.1
Pan troglodytes (chimpanzee)	PAPOLB	XP_003318331.1
Bos taurus (cattle)	PAPOLB	NP_001193351.1

Gene Ontology
Bibliography

Related articles in PubMed

Means to an end: mechanisms of alternative polyadenylation of messenger RNA precursors.
Gruber AR, Martin G, Keller W, Zavolan M
Wiley interdisciplinary reviews. RNA5(2)183-96(2014 Mar-Apr)

Germ cell-specific gene 1 targets testis-specific poly(A) polymerase to the endoplasmic reticulum through protein-protein interactions.
Choi HS, Lee SH, Kim H, Lee Y
FEBS letters582(8)1203-9(2008 Apr)

An intronless gene encoding a poly(A) polymerase is specifically expressed in testis.
Lee YJ, Lee Y, Chung JH
FEBS letters487(2)287-92(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Papolb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Papolb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa41837	NM_001012020.2 Latest version!	Rattus norvegicus poly(A) polymerase beta (Papolb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$377.30 $539.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Papolb ( NM_001012020.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001012020.1 Rattus norvegicus poly(A) polymerase beta (Papolb), mRNA.

Price & Availability↑

CloneID	ORa41837
Clone ID Related Accession (Same CDS sequence)	NM_001012020.2
Accession Version	NM_001012020.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1929bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Rattus norvegicus(Norway rat)
Product	poly(A) polymerase beta
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC079046.1 and CR458765.1. On Oct 19, 2005 this sequence version replaced NM_001012020.1. ##Evidence-Data-START## Transcript is intronless :: BC079046.1 [ECO:0000345] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATGCCAT TTGCGGTGAC CACCCAAGGA GCACAACAAC CGGCTCCCGC CCCGAAGCAG 
TACGGCATAT CGTCCCCCAT CAGCCTGGCC GCCCCCAAGG ACACAGACCG CGAACTCACC 
CAGAAGCTGA TCGAGACCCT CCAGCCCTTC GGGGTGTTTG AAGAGGAAGA GGAACTGCAG 
CGCAGGATTT TAATTTTGCA GAAATTAAAT AATCTGGTGA AGGAATGGAT CCGAGAAATC 
AGTGAAAGCA GGAATCTTCC ACAAGCTGTA ATTGAGAATG TTGGGGGGAA AATTTTTACG 
TTCGGCTCTT ACCGACTAGG AGTACACACG AAAGGTGCAG ATATCGATGC GTTGTGCGTT 
GCACCAAGAC ACGTGGATCG AAATGACTTT TTCACCTCCT TCTATGACAA ATTGAAACTA 
CAGGAGGAAG TAAAAGATTT AAGAGCGGTT GAGGAGGCCT TTGTGCCGGT TATCAAACTG 
TGTTTTGATG GAATAGAGAT TGATATTTTG TTTGCAAGAT TAGCATTGCA GACTATTCCG 
GAAGATTTGG ACCTACGAGA TGATAGTCTG CTTAAAAATT TAGATATTAG GTGCATAAGA 
AGCCTTAATG GTTGCCGGGT AACTGATGAA ATTTTACACC TAGTACCAAA CATTGACAGC 
TTCAGGTTAA CACTGAGAGC CATCAAATTA TGGGCCAAAT GCCACAACAT CTATTCCAAT 
ATATTGGGTT TCCTTGGAGG TGTTTCCTGG GCTATGCTGG TAGCAAGAAC TTGCCAGCTT 
TATCCAAATG CAATAGCATC CACTCTTGTA CGAAAATTTT TCTTGGTATT TTCTGAGTGG 
GAATGGCCAA ATCCAGTCTT GTTAAAAGAA CCAGAAGAAC GAAATCTTAA CTTGCCTGTA 
TGGGACCCAA GAGTGAATCC TAGTGACAGG TACCATCTTA TGCCTATAAT TACACCAGCA 
TACCCACAGC AGAACTCCAC ATACAATGTG TCTGTGTCAA CCAGGATGGT CATGATTGAG 
GAGTTTAAGC AAGGGCTTGC TATCACACAT GAAATTCTGC TGAATAAGGC AGAATGGTCC 
AAACTTTTTG AAGCTCCAAG CTTTTTTCAG AAATATAAGC ATTATATTGT ACTACTGGCA 
AGTGCACCAA CAGAGAAACA ACATTTAGAG TGGGTGGGCT TGGTGGAATC AAAGATCCGA 
ATCCTGGTTG GGAGCCTGGA AAAGAATGAA TTCATTACTC TGGCACATGT GAATCCCCAG 
TCATTTCCAG CACCTAAAGA AAATGTTGAC AAAGAAGAAT TTCGTACTAT GTGGGTGATT 
GGGTTAGTGT TGAAAAAGCC AGAAAATTCT GAAATTCTCA GTATTGATCT CACTTATGAT 
ATTCAGTCTT TCACAGATAC AGTGTACAGA CAAGCAATAA ATAGTAAGAT GTTTGAGATG 
GATATGAAGA TTGCTGCAAT GCATCTAAGA AGAAAGGAAC TTCATCAGCT ACTTCCAAAT 
CATGTTCTTC AGAAAAAGGA AACACATTTA ACAGAAGGTG TAAGATTGAC AGCTGTGAAT 
GATAGCAGCC TTCTCTTGTC TATAGACAGT GAAAACAGCA TGACTGCACC TTCACCTACT 
GGCACTATAA AGACAGGCCC ACTGACTGCA AACCCTCAGG GCAGAAACAG TCCTGCTCTG 
GCTGTTATGG CAGCATCCGT GACCAACATA CAGTTTCCTG ACGTTTCCTT GCAGCACGTG 
AACCCCATAG AAAGTTCAGG GATTGCACTG AGTGAAAGCA TTGCTCAGAT TCCCTCACAG 
CCGACCATCT CACCACCACC TAAGCCTACC ATGACCAGAG TTGTTTCTTC GACACATCTG 
GTAAACCATC CATCTAGACC TTCAGGAAAT ACAGCAACAA ACATACCCAA TCCTATACTA 
GGAGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001012020.1
CDS	203..2131
Translation	MMPFAVTTQGAQQPAPAPKQYGISSPISLAAPKDTDRELTQKLIETLQPFGVFEEEEELQRRILILQKLNNLVKEWIREISESRNLPQAVIENVGGKIFTFGSYRLGVHTKGADIDALCVAPRHVDRNDFFTSFYDKLKLQEEVKDLRAVEEAFVPVIKLCFDGIEIDILFARLALQTIPEDLDLRDDSLLKNLDIRCIRSLNGCRVTDEILHLVPNIDSFRLTLRAIKLWAKCHNIYSNILGFLGGVSWAMLVARTCQLYPNAIASTLVRKFFLVFSEWEWPNPVLLKEPEERNLNLPVWDPRVNPSDRYHLMPIITPAYPQQNSTYNVSVSTRMVMIEEFKQGLAITHEILLNKAEWSKLFEAPSFFQKYKHYIVLLASAPTEKQHLEWVGLVESKIRILVGSLEKNEFITLAHVNPQSFPAPKENVDKEEFRTMWVIGLVLKKPENSEILSIDLTYDIQSFTDTVYRQAINSKMFEMDMKIAAMHLRRKELHQLLPNHVLQKKETHLTEGVRLTAVNDSSLLLSIDSENSMTAPSPTGTIKTGPLTANPQGRNSPALAVMAASVTNIQFPDVSLQHVNPIESSGIALSESIAQIPSQPTISPPPKPTMTRVVSSTHLVNHPSRPSGNTATNIPNPILGV

Target ORF information:

RefSeq Version	NM_001012020.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus poly(A) polymerase beta (Papolb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001012020.2

ORF Insert Sequence:

ATGATGCCAT TTGCGGTGAC CACCCAAGGA GCACAACAAC CGGCTCCCGC CCCGAAGCAG 
TACGGCATAT CGTCCCCCAT CAGCCTGGCC GCCCCCAAGG ACACAGACCG CGAACTCACC 
CAGAAGCTGA TCGAGACCCT CCAGCCCTTC GGGGTGTTTG AAGAGGAAGA GGAACTGCAG 
CGCAGGATTT TAATTTTGCA GAAATTAAAT AATCTGGTGA AGGAATGGAT CCGAGAAATC 
AGTGAAAGCA GGAATCTTCC ACAAGCTGTA ATTGAGAATG TTGGGGGGAA AATTTTTACG 
TTCGGCTCTT ACCGACTAGG AGTACACACG AAAGGTGCAG ATATCGATGC GTTGTGCGTT 
GCACCAAGAC ACGTGGATCG AAATGACTTT TTCACCTCCT TCTATGACAA ATTGAAACTA 
CAGGAGGAAG TAAAAGATTT AAGAGCGGTT GAGGAGGCCT TTGTGCCGGT TATCAAACTG 
TGTTTTGATG GAATAGAGAT TGATATTTTG TTTGCAAGAT TAGCATTGCA GACTATTCCG 
GAAGATTTGG ACCTACGAGA TGATAGTCTG CTTAAAAATT TAGATATTAG GTGCATAAGA 
AGCCTTAATG GTTGCCGGGT AACTGATGAA ATTTTACACC TAGTACCAAA CATTGACAGC 
TTCAGGTTAA CACTGAGAGC CATCAAATTA TGGGCCAAAT GCCACAACAT CTATTCCAAT 
ATATTGGGTT TCCTTGGAGG TGTTTCCTGG GCTATGCTGG TAGCAAGAAC TTGCCAGCTT 
TATCCAAATG CAATAGCATC CACTCTTGTA CGAAAATTTT TCTTGGTATT TTCTGAGTGG 
GAATGGCCAA ATCCAGTCTT GTTAAAAGAA CCAGAAGAAC GAAATCTTAA CTTGCCTGTA 
TGGGACCCAA GAGTGAATCC TAGTGACAGG TACCATCTTA TGCCTATAAT TACACCAGCA 
TACCCACAGC AGAACTCCAC ATACAATGTG TCTGTGTCAA CCAGGATGGT CATGATTGAG 
GAGTTTAAGC AAGGGCTTGC TATCACACAT GAAATTCTGC TGAATAAGGC AGAATGGTCC 
AAACTTTTTG AAGCTCCAAG CTTTTTTCAG AAATATAAGC ATTATATTGT ACTACTGGCA 
AGTGCACCAA CAGAGAAACA ACATTTAGAG TGGGTGGGCT TGGTGGAATC AAAGATCCGA 
ATCCTGGTTG GGAGCCTGGA AAAGAATGAA TTCATTACTC TGGCACATGT GAATCCCCAG 
TCATTTCCAG CACCTAAAGA AAATGTTGAC AAAGAAGAAT TTCGTACTAT GTGGGTGATT 
GGGTTAGTGT TGAAAAAGCC AGAAAATTCT GAAATTCTCA GTATTGATCT CACTTATGAT 
ATTCAGTCTT TCACAGATAC AGTGTACAGA CAAGCAATAA ATAGTAAGAT GTTTGAGATG 
GATATGAAGA TTGCTGCAAT GCATCTAAGA AGAAAGGAAC TTCATCAGCT ACTTCCAAAT 
CATGTTCTTC AGAAAAAGGA AACACATTTA ACAGAAGGTG TAAGATTGAC AGCTGTGAAT 
GATAGCAGCC TTCTCTTGTC TATAGACAGT GAAAACAGCA TGACTGCACC TTCACCTACT 
GGCACTATAA AGACAGGCCC ACTGACTGCA AACCCTCAGG GCAGAAACAG TCCTGCTCTG 
GCTGTTATGG CAGCATCCGT GACCAACATA CAGTTTCCTG ACGTTTCCTT GCAGCACGTG 
AACCCCATAG AAAGTTCAGG GATTGCACTG AGTGAAAGCA TTGCTCAGAT TCCCTCACAG 
CCGACCATCT CACCACCACC TAAGCCTACC ATGACCAGAG TTGTTTCTTC GACACATCTG 
GTAAACCATC CATCTAGACC TTCAGGAAAT ACAGCAACAA ACATACCCAA TCCTATACTA 
GGAGTCTAG