Krt25 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Krt25
Entrez Gene ID	303519
Full Name	keratin 25
Synonyms	Ka38,Krt25A
General protein information	Preferred Names keratin 25 Names keratin, type I cytoskeletal 25 CK-25 K25 K25A cytokeratin-25 keratin 25, type I keratin-25A type I inner root sheath-specific keratin-K25irs1 type I keratin KA38
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q31

mRNA and Protein(s)

mRNA	Protein	Name
XM_017597319.1	XP_017452808.1	keratin, type I cytoskeletal 25 isoform X1
NM_001008822.1	NP_001008822.1	keratin, type I cytoskeletal 25
XM_017597318.1	XP_017452807.1	keratin, type I cytoskeletal 25 isoform X1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Krt25	NP_001008822.1
Pan troglodytes (chimpanzee)	KRT25	NP_001092033.1
	KRT25	XP_001100293.1
Homo sapiens (human)	KRT25	NP_853512.1
Canis lupus familiaris (dog)	KRT25	XP_003435295.1
Bos taurus (cattle)	KRT25	NP_001068991.1
Mus musculus (house mouse)	Krt25	NP_598491.1

Gene Ontology
Bibliography

Related articles in PubMed

A Missense Mutation within the Helix Termination Motif of KRT25 Causes Autosomal Dominant Woolly Hair/Hypotrichosis.
Yu X, Chen F, Ni C, Zhang G, Zheng L, Zhang J, Li C, Sandilands A, Yao Z, Li M
The Journal of investigative dermatology138(1)230-233(2018 Jan)

Autosomal Recessive Hypotrichosis with Woolly Hair Caused by a Mutation in the Keratin 25 Gene Expressed in Hair Follicles.
Zernov NV, Skoblov MY, Marakhonov AV, Shimomura Y, Vasilyeva TA, Konovalov FA, Abrukova AV, Zinchenko RA
The Journal of investigative dermatology136(6)1097-105(2016 Jun)

Hair greying is associated with active hair growth.
Choi HI, Choi GI, Kim EK, Choi YJ, Sohn KC, Lee Y, Kim CD, Yoon TJ, Sohn HJ, Han SH, Kim S, Lee JH, Lee YH
The British journal of dermatology165(6)1183-9(2011 Dec)

Mutations in the helix termination motif of mouse type I IRS keratin genes impair the assembly of keratin intermediate filament.
Tanaka S, Miura I, Yoshiki A, Kato Y, Yokoyama H, Shinogi A, Masuya H, Wakana S, Tamura M, Shiroishi T
Genomics90(6)703-11(2007 Dec)

The human type I keratin gene family: characterization of new hair follicle specific members and evaluation of the chromosome 17q21.2 gene domain.
Rogers MA, Winter H, Langbein L, Bleiler R, Schweizer J
Differentiation; research in biological diversity72(9-10)527-40(2004 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Krt25 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Krt25 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa05863	NM_001008822.1 Latest version!	Rattus norvegicus keratin 25 (Krt25), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa05863	XM_017597318.1 Latest version!	Rattus norvegicus keratin 25 (Krt25), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa05863	XM_017597319.1 Latest version!	Rattus norvegicus keratin 25 (Krt25), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Krt25 ( NM_001008822.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001008822.1 Rattus norvegicus keratin 25 (Krt25), mRNA.

Price & Availability↑

CloneID	ORa05863
Clone ID Related Accession (Same CDS sequence)	XM_017597319.1 , NM_001008822.1 , XM_017597318.1
Accession Version	NM_001008822.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1341bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Rattus norvegicus(Norway rat)
Product	keratin, type I cytoskeletal 25
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BK004028.1. On Jan 5, 2005 this sequence version replaced XM_220938.2. ##Evidence-Data-START## Transcript exon combination :: BK004028.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN02642623, SAMN02642629 [ECO:0000348] ##Evidence-Data-END##

ATGTCTCTTC GCCTCTCCAG TGGATCCAAG AGGTCCTATG CTCGTCCCAG CACAGGATCG 
CTCAGGGGCG CCAGCTTTGG TGCTGCAAAT GCTTGTGGCG TAGCAGGCAT TGGAAGTGGC 
TTCTCCTGCG CCTTTGGGAG CAGCTCCACA GGAGGAAACA CGGGTGTGGC CAACTCCTGT 
GCTGGCTTCA CTGTGAATGA GGGGGGCCTC CTCTCCGGCA ATGAGAAGGT GACCATGCAG 
AACCTCAATG ACCGCCTGGC CTCCTACCTG GATAATGTAC AAGCACTTCA GGAGGCCAAC 
GCTGACCTGG AGCAGAAGAT CAAGGGCTGG TATGAAAAAT TTGGACCTGG GTCATGCCGC 
GGTCTCGATC ATGACTACAG TAGATACTTC CCCATCATTG ATGATCTTAA AAACCAGATC 
ATCACTTCCA CCACCAGCAA TGCCAATGCC GTTCTGCAGA TTGACAATGC CAGGCTCACG 
GCTGATGACT TCAGACTCAA GTACGAAAAC GAGCTGGCCC TCCACCAGAG CGTAGAGGCT 
GACGTGAACG GGCTGCGCAG GGTTCTGGAT GAAATCACCC TGTGCCGCAC TGACCTGGAG 
ATTCAGTACG AAACCCTGAG TGAGGAACTG ACTTACCTCA AGAAGAACCA CAAAGAGGAA 
ATGCAGGCTC TGCAGTGCGC AGCCGGAGGT AATGTGAATG TGGAGATGAA CGCAGCCCCC 
GGCGTGGACC TCACCGTCCT GCTGAACAAC ATGCGAGCCG AGTACGAGGC CCTGGCTGAG 
CAGAACCGCA GGGACGCCGA GGCCTGGTTC CAAGAGAAGA GCGCTTCGCT GCAGCAGCAG 
ATCACCGAGG ACGTGGGCGC CACCACCTCA GCCAGGAATG AGCTGACGGA AATGAAACGT 
ACGCTCCAAA CCTTGGAGAT TGAACTACAG TCTCTCCTGG CCACGAAACA CTCTCTGGAG 
TGCTCCCTGA CGGAGACCGA GGGCAACTAC TGCACGCAGC TGGCTCAGAT CCAGGCTCAG 
ATCAGCGCCC TGGAGGAGCA GCTGCACCAG GTGAGGACGG AGACCGAGGG CCAGAAGCTG 
GAATATGAGC AGCTGCTGAA CGTCAAGGCC CACCTGGAGA AGGAGATCGA GACCTACTGC 
CTCCTCATAG GAGGAGACGA GGGGGCCTGT AAGTCTTCGA GTTACAAGTC TAAAGATTAC 
ACCTCTGGAA ACGCGGGAAA CCAAAGCAAA GATTCCCCCA AAGCCATAGT GGTTAAGAAA 
GTCCTAGAGG AGGTGGACCA ACGCAGCAAG ATTCTAACCA CTAGGCTCCA CTCCCTGGAA 
GAGAAATCTC AAAGCAATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001008822.1
CDS	1..1341
Translation	MSLRLSSGSKRSYARPSTGSLRGASFGAANACGVAGIGSGFSCAFGSSSTGGNTGVANSCAGFTVNEGGLLSGNEKVTMQNLNDRLASYLDNVQALQEANADLEQKIKGWYEKFGPGSCRGLDHDYSRYFPIIDDLKNQIITSTTSNANAVLQIDNARLTADDFRLKYENELALHQSVEADVNGLRRVLDEITLCRTDLEIQYETLSEELTYLKKNHKEEMQALQCAAGGNVNVEMNAAPGVDLTVLLNNMRAEYEALAEQNRRDAEAWFQEKSASLQQQITEDVGATTSARNELTEMKRTLQTLEIELQSLLATKHSLECSLTETEGNYCTQLAQIQAQISALEEQLHQVRTETEGQKLEYEQLLNVKAHLEKEIETYCLLIGGDEGACKSSSYKSKDYTSGNAGNQSKDSPKAIVVKKVLEEVDQRSKILTTRLHSLEEKSQSN

Target ORF information:

RefSeq Version	NM_001008822.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus keratin 25 (Krt25), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001008822.1

ORF Insert Sequence:

ATGTCTCTTC GCCTCTCCAG TGGATCCAAG AGGTCCTATG CTCGTCCCAG CACAGGATCG 
CTCAGGGGCG CCAGCTTTGG TGCTGCAAAT GCTTGTGGCG TAGCAGGCAT TGGAAGTGGC 
TTCTCCTGCG CCTTTGGGAG CAGCTCCACA GGAGGAAACA CGGGTGTGGC CAACTCCTGT 
GCTGGCTTCA CTGTGAATGA GGGGGGCCTC CTCTCCGGCA ATGAGAAGGT GACCATGCAG 
AACCTCAATG ACCGCCTGGC CTCCTACCTG GATAATGTAC AAGCACTTCA GGAGGCCAAC 
GCTGACCTGG AGCAGAAGAT CAAGGGCTGG TATGAAAAAT TTGGACCTGG GTCATGCCGC 
GGTCTCGATC ATGACTACAG TAGATACTTC CCCATCATTG ATGATCTTAA AAACCAGATC 
ATCACTTCCA CCACCAGCAA TGCCAATGCC GTTCTGCAGA TTGACAATGC CAGGCTCACG 
GCTGATGACT TCAGACTCAA GTACGAAAAC GAGCTGGCCC TCCACCAGAG CGTAGAGGCT 
GACGTGAACG GGCTGCGCAG GGTTCTGGAT GAAATCACCC TGTGCCGCAC TGACCTGGAG 
ATTCAGTACG AAACCCTGAG TGAGGAACTG ACTTACCTCA AGAAGAACCA CAAAGAGGAA 
ATGCAGGCTC TGCAGTGCGC AGCCGGAGGT AATGTGAATG TGGAGATGAA CGCAGCCCCC 
GGCGTGGACC TCACCGTCCT GCTGAACAAC ATGCGAGCCG AGTACGAGGC CCTGGCTGAG 
CAGAACCGCA GGGACGCCGA GGCCTGGTTC CAAGAGAAGA GCGCTTCGCT GCAGCAGCAG 
ATCACCGAGG ACGTGGGCGC CACCACCTCA GCCAGGAATG AGCTGACGGA AATGAAACGT 
ACGCTCCAAA CCTTGGAGAT TGAACTACAG TCTCTCCTGG CCACGAAACA CTCTCTGGAG 
TGCTCCCTGA CGGAGACCGA GGGCAACTAC TGCACGCAGC TGGCTCAGAT CCAGGCTCAG 
ATCAGCGCCC TGGAGGAGCA GCTGCACCAG GTGAGGACGG AGACCGAGGG CCAGAAGCTG 
GAATATGAGC AGCTGCTGAA CGTCAAGGCC CACCTGGAGA AGGAGATCGA GACCTACTGC 
CTCCTCATAG GAGGAGACGA GGGGGCCTGT AAGTCTTCGA GTTACAAGTC TAAAGATTAC 
ACCTCTGGAA ACGCGGGAAA CCAAAGCAAA GATTCCCCCA AAGCCATAGT GGTTAAGAAA 
GTCCTAGAGG AGGTGGACCA ACGCAGCAAG ATTCTAACCA CTAGGCTCCA CTCCCTGGAA 
GAGAAATCTC AAAGCAATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Krt25 ( XM_017597319.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017452808.1 Rattus norvegicus keratin 25 (Krt25), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa05863
Clone ID Related Accession (Same CDS sequence)	XM_017597319.1 , NM_001008822.1 , XM_017597318.1
Accession Version	XM_017597319.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1341bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	keratin, type I cytoskeletal 25 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCTTC GCCTCTCCAG TGGATCCAAG AGGTCCTATG CTCGTCCCAG CACAGGATCG 
CTCAGGGGCG CCAGCTTTGG TGCTGCAAAT GCTTGTGGCG TAGCAGGCAT TGGAAGTGGC 
TTCTCCTGCG CCTTTGGGAG CAGCTCCACA GGAGGAAACA CGGGTGTGGC CAACTCCTGT 
GCTGGCTTCA CTGTGAATGA GGGGGGCCTC CTCTCCGGCA ATGAGAAGGT GACCATGCAG 
AACCTCAATG ACCGCCTGGC CTCCTACCTG GATAATGTAC AAGCACTTCA GGAGGCCAAC 
GCTGACCTGG AGCAGAAGAT CAAGGGCTGG TATGAAAAAT TTGGACCTGG GTCATGCCGC 
GGTCTCGATC ATGACTACAG TAGATACTTC CCCATCATTG ATGATCTTAA AAACCAGATC 
ATCACTTCCA CCACCAGCAA TGCCAATGCC GTTCTGCAGA TTGACAATGC CAGGCTCACG 
GCTGATGACT TCAGACTCAA GTACGAAAAC GAGCTGGCCC TCCACCAGAG CGTAGAGGCT 
GACGTGAACG GGCTGCGCAG GGTTCTGGAT GAAATCACCC TGTGCCGCAC TGACCTGGAG 
ATTCAGTACG AAACCCTGAG TGAGGAACTG ACTTACCTCA AGAAGAACCA CAAAGAGGAA 
ATGCAGGCTC TGCAGTGCGC AGCCGGAGGT AATGTGAATG TGGAGATGAA CGCAGCCCCC 
GGCGTGGACC TCACCGTCCT GCTGAACAAC ATGCGAGCCG AGTACGAGGC CCTGGCTGAG 
CAGAACCGCA GGGACGCCGA GGCCTGGTTC CAAGAGAAGA GCGCTTCGCT GCAGCAGCAG 
ATCACCGAGG ACGTGGGCGC CACCACCTCA GCCAGGAATG AGCTGACGGA AATGAAACGT 
ACGCTCCAAA CCTTGGAGAT TGAACTACAG TCTCTCCTGG CCACGAAACA CTCTCTGGAG 
TGCTCCCTGA CGGAGACCGA GGGCAACTAC TGCACGCAGC TGGCTCAGAT CCAGGCTCAG 
ATCAGCGCCC TGGAGGAGCA GCTGCACCAG GTGAGGACGG AGACCGAGGG CCAGAAGCTG 
GAATATGAGC AGCTGCTGAA CGTCAAGGCC CACCTGGAGA AGGAGATCGA GACCTACTGC 
CTCCTCATAG GAGGAGACGA GGGGGCCTGT AAGTCTTCGA GTTACAAGTC TAAAGATTAC 
ACCTCTGGAA ACGCGGGAAA CCAAAGCAAA GATTCCCCCA AAGCCATAGT GGTTAAGAAA 
GTCCTAGAGG AGGTGGACCA ACGCAGCAAG ATTCTAACCA CTAGGCTCCA CTCCCTGGAA 
GAGAAATCTC AAAGCAATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017452808.1
CDS	1625..2965
Translation	MSLRLSSGSKRSYARPSTGSLRGASFGAANACGVAGIGSGFSCAFGSSSTGGNTGVANSCAGFTVNEGGLLSGNEKVTMQNLNDRLASYLDNVQALQEANADLEQKIKGWYEKFGPGSCRGLDHDYSRYFPIIDDLKNQIITSTTSNANAVLQIDNARLTADDFRLKYENELALHQSVEADVNGLRRVLDEITLCRTDLEIQYETLSEELTYLKKNHKEEMQALQCAAGGNVNVEMNAAPGVDLTVLLNNMRAEYEALAEQNRRDAEAWFQEKSASLQQQITEDVGATTSARNELTEMKRTLQTLEIELQSLLATKHSLECSLTETEGNYCTQLAQIQAQISALEEQLHQVRTETEGQKLEYEQLLNVKAHLEKEIETYCLLIGGDEGACKSSSYKSKDYTSGNAGNQSKDSPKAIVVKKVLEEVDQRSKILTTRLHSLEEKSQSN

Target ORF information:

RefSeq Version	XM_017597319.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus keratin 25 (Krt25), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017597319.1

ORF Insert Sequence:

ATGTCTCTTC GCCTCTCCAG TGGATCCAAG AGGTCCTATG CTCGTCCCAG CACAGGATCG 
CTCAGGGGCG CCAGCTTTGG TGCTGCAAAT GCTTGTGGCG TAGCAGGCAT TGGAAGTGGC 
TTCTCCTGCG CCTTTGGGAG CAGCTCCACA GGAGGAAACA CGGGTGTGGC CAACTCCTGT 
GCTGGCTTCA CTGTGAATGA GGGGGGCCTC CTCTCCGGCA ATGAGAAGGT GACCATGCAG 
AACCTCAATG ACCGCCTGGC CTCCTACCTG GATAATGTAC AAGCACTTCA GGAGGCCAAC 
GCTGACCTGG AGCAGAAGAT CAAGGGCTGG TATGAAAAAT TTGGACCTGG GTCATGCCGC 
GGTCTCGATC ATGACTACAG TAGATACTTC CCCATCATTG ATGATCTTAA AAACCAGATC 
ATCACTTCCA CCACCAGCAA TGCCAATGCC GTTCTGCAGA TTGACAATGC CAGGCTCACG 
GCTGATGACT TCAGACTCAA GTACGAAAAC GAGCTGGCCC TCCACCAGAG CGTAGAGGCT 
GACGTGAACG GGCTGCGCAG GGTTCTGGAT GAAATCACCC TGTGCCGCAC TGACCTGGAG 
ATTCAGTACG AAACCCTGAG TGAGGAACTG ACTTACCTCA AGAAGAACCA CAAAGAGGAA 
ATGCAGGCTC TGCAGTGCGC AGCCGGAGGT AATGTGAATG TGGAGATGAA CGCAGCCCCC 
GGCGTGGACC TCACCGTCCT GCTGAACAAC ATGCGAGCCG AGTACGAGGC CCTGGCTGAG 
CAGAACCGCA GGGACGCCGA GGCCTGGTTC CAAGAGAAGA GCGCTTCGCT GCAGCAGCAG 
ATCACCGAGG ACGTGGGCGC CACCACCTCA GCCAGGAATG AGCTGACGGA AATGAAACGT 
ACGCTCCAAA CCTTGGAGAT TGAACTACAG TCTCTCCTGG CCACGAAACA CTCTCTGGAG 
TGCTCCCTGA CGGAGACCGA GGGCAACTAC TGCACGCAGC TGGCTCAGAT CCAGGCTCAG 
ATCAGCGCCC TGGAGGAGCA GCTGCACCAG GTGAGGACGG AGACCGAGGG CCAGAAGCTG 
GAATATGAGC AGCTGCTGAA CGTCAAGGCC CACCTGGAGA AGGAGATCGA GACCTACTGC 
CTCCTCATAG GAGGAGACGA GGGGGCCTGT AAGTCTTCGA GTTACAAGTC TAAAGATTAC 
ACCTCTGGAA ACGCGGGAAA CCAAAGCAAA GATTCCCCCA AAGCCATAGT GGTTAAGAAA 
GTCCTAGAGG AGGTGGACCA ACGCAGCAAG ATTCTAACCA CTAGGCTCCA CTCCCTGGAA 
GAGAAATCTC AAAGCAATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Krt25 ( XM_017597318.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017452807.1 Rattus norvegicus keratin 25 (Krt25), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa05863
Clone ID Related Accession (Same CDS sequence)	XM_017597319.1 , NM_001008822.1 , XM_017597318.1
Accession Version	XM_017597318.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1341bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	keratin, type I cytoskeletal 25 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCTTC GCCTCTCCAG TGGATCCAAG AGGTCCTATG CTCGTCCCAG CACAGGATCG 
CTCAGGGGCG CCAGCTTTGG TGCTGCAAAT GCTTGTGGCG TAGCAGGCAT TGGAAGTGGC 
TTCTCCTGCG CCTTTGGGAG CAGCTCCACA GGAGGAAACA CGGGTGTGGC CAACTCCTGT 
GCTGGCTTCA CTGTGAATGA GGGGGGCCTC CTCTCCGGCA ATGAGAAGGT GACCATGCAG 
AACCTCAATG ACCGCCTGGC CTCCTACCTG GATAATGTAC AAGCACTTCA GGAGGCCAAC 
GCTGACCTGG AGCAGAAGAT CAAGGGCTGG TATGAAAAAT TTGGACCTGG GTCATGCCGC 
GGTCTCGATC ATGACTACAG TAGATACTTC CCCATCATTG ATGATCTTAA AAACCAGATC 
ATCACTTCCA CCACCAGCAA TGCCAATGCC GTTCTGCAGA TTGACAATGC CAGGCTCACG 
GCTGATGACT TCAGACTCAA GTACGAAAAC GAGCTGGCCC TCCACCAGAG CGTAGAGGCT 
GACGTGAACG GGCTGCGCAG GGTTCTGGAT GAAATCACCC TGTGCCGCAC TGACCTGGAG 
ATTCAGTACG AAACCCTGAG TGAGGAACTG ACTTACCTCA AGAAGAACCA CAAAGAGGAA 
ATGCAGGCTC TGCAGTGCGC AGCCGGAGGT AATGTGAATG TGGAGATGAA CGCAGCCCCC 
GGCGTGGACC TCACCGTCCT GCTGAACAAC ATGCGAGCCG AGTACGAGGC CCTGGCTGAG 
CAGAACCGCA GGGACGCCGA GGCCTGGTTC CAAGAGAAGA GCGCTTCGCT GCAGCAGCAG 
ATCACCGAGG ACGTGGGCGC CACCACCTCA GCCAGGAATG AGCTGACGGA AATGAAACGT 
ACGCTCCAAA CCTTGGAGAT TGAACTACAG TCTCTCCTGG CCACGAAACA CTCTCTGGAG 
TGCTCCCTGA CGGAGACCGA GGGCAACTAC TGCACGCAGC TGGCTCAGAT CCAGGCTCAG 
ATCAGCGCCC TGGAGGAGCA GCTGCACCAG GTGAGGACGG AGACCGAGGG CCAGAAGCTG 
GAATATGAGC AGCTGCTGAA CGTCAAGGCC CACCTGGAGA AGGAGATCGA GACCTACTGC 
CTCCTCATAG GAGGAGACGA GGGGGCCTGT AAGTCTTCGA GTTACAAGTC TAAAGATTAC 
ACCTCTGGAA ACGCGGGAAA CCAAAGCAAA GATTCCCCCA AAGCCATAGT GGTTAAGAAA 
GTCCTAGAGG AGGTGGACCA ACGCAGCAAG ATTCTAACCA CTAGGCTCCA CTCCCTGGAA 
GAGAAATCTC AAAGCAATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017452807.1
CDS	1615..2955
Translation	MSLRLSSGSKRSYARPSTGSLRGASFGAANACGVAGIGSGFSCAFGSSSTGGNTGVANSCAGFTVNEGGLLSGNEKVTMQNLNDRLASYLDNVQALQEANADLEQKIKGWYEKFGPGSCRGLDHDYSRYFPIIDDLKNQIITSTTSNANAVLQIDNARLTADDFRLKYENELALHQSVEADVNGLRRVLDEITLCRTDLEIQYETLSEELTYLKKNHKEEMQALQCAAGGNVNVEMNAAPGVDLTVLLNNMRAEYEALAEQNRRDAEAWFQEKSASLQQQITEDVGATTSARNELTEMKRTLQTLEIELQSLLATKHSLECSLTETEGNYCTQLAQIQAQISALEEQLHQVRTETEGQKLEYEQLLNVKAHLEKEIETYCLLIGGDEGACKSSSYKSKDYTSGNAGNQSKDSPKAIVVKKVLEEVDQRSKILTTRLHSLEEKSQSN

Target ORF information:

RefSeq Version	XM_017597318.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus keratin 25 (Krt25), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017597318.1

ORF Insert Sequence:

ATGTCTCTTC GCCTCTCCAG TGGATCCAAG AGGTCCTATG CTCGTCCCAG CACAGGATCG 
CTCAGGGGCG CCAGCTTTGG TGCTGCAAAT GCTTGTGGCG TAGCAGGCAT TGGAAGTGGC 
TTCTCCTGCG CCTTTGGGAG CAGCTCCACA GGAGGAAACA CGGGTGTGGC CAACTCCTGT 
GCTGGCTTCA CTGTGAATGA GGGGGGCCTC CTCTCCGGCA ATGAGAAGGT GACCATGCAG 
AACCTCAATG ACCGCCTGGC CTCCTACCTG GATAATGTAC AAGCACTTCA GGAGGCCAAC 
GCTGACCTGG AGCAGAAGAT CAAGGGCTGG TATGAAAAAT TTGGACCTGG GTCATGCCGC 
GGTCTCGATC ATGACTACAG TAGATACTTC CCCATCATTG ATGATCTTAA AAACCAGATC 
ATCACTTCCA CCACCAGCAA TGCCAATGCC GTTCTGCAGA TTGACAATGC CAGGCTCACG 
GCTGATGACT TCAGACTCAA GTACGAAAAC GAGCTGGCCC TCCACCAGAG CGTAGAGGCT 
GACGTGAACG GGCTGCGCAG GGTTCTGGAT GAAATCACCC TGTGCCGCAC TGACCTGGAG 
ATTCAGTACG AAACCCTGAG TGAGGAACTG ACTTACCTCA AGAAGAACCA CAAAGAGGAA 
ATGCAGGCTC TGCAGTGCGC AGCCGGAGGT AATGTGAATG TGGAGATGAA CGCAGCCCCC 
GGCGTGGACC TCACCGTCCT GCTGAACAAC ATGCGAGCCG AGTACGAGGC CCTGGCTGAG 
CAGAACCGCA GGGACGCCGA GGCCTGGTTC CAAGAGAAGA GCGCTTCGCT GCAGCAGCAG 
ATCACCGAGG ACGTGGGCGC CACCACCTCA GCCAGGAATG AGCTGACGGA AATGAAACGT 
ACGCTCCAAA CCTTGGAGAT TGAACTACAG TCTCTCCTGG CCACGAAACA CTCTCTGGAG 
TGCTCCCTGA CGGAGACCGA GGGCAACTAC TGCACGCAGC TGGCTCAGAT CCAGGCTCAG 
ATCAGCGCCC TGGAGGAGCA GCTGCACCAG GTGAGGACGG AGACCGAGGG CCAGAAGCTG 
GAATATGAGC AGCTGCTGAA CGTCAAGGCC CACCTGGAGA AGGAGATCGA GACCTACTGC 
CTCCTCATAG GAGGAGACGA GGGGGCCTGT AAGTCTTCGA GTTACAAGTC TAAAGATTAC 
ACCTCTGGAA ACGCGGGAAA CCAAAGCAAA GATTCCCCCA AAGCCATAGT GGTTAAGAAA 
GTCCTAGAGG AGGTGGACCA ACGCAGCAAG ATTCTAACCA CTAGGCTCCA CTCCCTGGAA 
GAGAAATCTC AAAGCAATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A Missense Mutation within the Helix Termination Motif of KRT25 Causes Autosomal Dominant Woolly Hair/Hypotrichosis.
The Journal of investigative dermatology138(1)230-233(2018 Jan)
Yu X,Chen F,Ni C,Zhang G,Zheng L,Zhang J,Li C,Sandilands A,Yao Z,Li M

Autosomal Recessive Hypotrichosis with Woolly Hair Caused by a Mutation in the Keratin 25 Gene Expressed in Hair Follicles.
The Journal of investigative dermatology136(6)1097-105(2016 Jun)
Zernov NV,Skoblov MY,Marakhonov AV,Shimomura Y,Vasilyeva TA,Konovalov FA,Abrukova AV,Zinchenko RA

Hair greying is associated with active hair growth.
The British journal of dermatology165(6)1183-9(2011 Dec)
Choi HI,Choi GI,Kim EK,Choi YJ,Sohn KC,Lee Y,Kim CD,Yoon TJ,Sohn HJ,Han SH,Kim S,Lee JH,Lee YH

Mutations in the helix termination motif of mouse type I IRS keratin genes impair the assembly of keratin intermediate filament.
Genomics90(6)703-11(2007 Dec)
Tanaka S,Miura I,Yoshiki A,Kato Y,Yokoyama H,Shinogi A,Masuya H,Wakana S,Tamura M,Shiroishi T

The human type I keratin gene family: characterization of new hair follicle specific members and evaluation of the chromosome 17q21.2 gene domain.
Differentiation; research in biological diversity72(9-10)527-40(2004 Dec)
Rogers MA,Winter H,Langbein L,Bleiler R,Schweizer J