Rrm2b cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rrm2b
Entrez Gene ID	299976
Full Name	ribonucleotide reductase regulatory TP53 inducible subunit M2B
General protein information	Preferred Names ribonucleotide reductase regulatory TP53 inducible subunit M2B Names ribonucleoside-diphosphate reductase subunit M2 B ribonucleotide reductase M2 B (TP53 inducible)
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 7 Map Location: 7q22

mRNA and Protein(s)

mRNA	Protein	Name
NM_001130543.1	NP_001124015.1	ribonucleoside-diphosphate reductase subunit M2 B

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	RRM2B	XP_539109.3
Mus musculus (house mouse)	Rrm2b	NP_955770.1
Homo sapiens (human)	RRM2B	NP_001165948.1
Gallus gallus (chicken)	RRM2B	XP_418364.2
Pan troglodytes (chimpanzee)	RRM2B	XP_001153916.1
Bos taurus (cattle)	RRM2B	XP_002692807.1
Danio rerio (zebrafish)	rrm2b	NP_001007164.1
Arabidopsis thaliana (thale cress)	RNR2A	NP_189000.1
Rattus norvegicus (Norway rat)	Rrm2b	NP_001124015.1
Xenopus tropicalis (tropical clawed frog)	rrm2b	NP_001119973.1

Gene Ontology
Bibliography

Related articles in PubMed

Mutation of RRM2B, encoding p53-controlled ribonucleotide reductase (p53R2), causes severe mitochondrial DNA depletion.
Bourdon A, Minai L, Serre V, Jais JP, Sarzi E, Aubert S, Chr?tien D, de Lonlay P, Paquis-Flucklinger V, Arakawa H, Nakamura Y, Munnich A, R?tig A
Nature genetics39(6)776-80(2007 Jun)

Strong induction of p73 protein in vivo coincides with the onset of apoptosis in rat liver after treatment with the hepatocarcinogen N-nitrosomorpholine (NNM).
Tudzarova-Trajkovska S, Wesierska-Gadek J
Journal of cellular biochemistry90(4)837-55(2003 Nov)

Impaired function of p53R2 in Rrm2b-null mice causes severe renal failure through attenuation of dNTP pools.
Kimura T, Takeda S, Sagiya Y, Gotoh M, Nakamura Y, Arakawa H
Nature genetics34(4)440-5(2003 Aug)

Mammalian p53R2 protein forms an active ribonucleotide reductase in vitro with the R1 protein, which is expressed both in resting cells in response to DNA damage and in proliferating cells.
Guittet O, H?kansson P, Voevodskaya N, Fridd S, Gr?slund A, Arakawa H, Nakamura Y, Thelander L
The Journal of biological chemistry276(44)40647-51(2001 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Rrm2b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rrm2b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa02506	NM_001130543.1 Latest version!	Rattus norvegicus ribonucleotide reductase regulatory TP53 inducible subunit M2B (Rrm2b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rrm2b ( NM_001130543.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001124015.1 Rattus norvegicus ribonucleotide reductase regulatory TP53 inducible subunit M2B (Rrm2b), mRNA.

Price & Availability↑

CloneID	ORa02506
Clone ID Related Accession (Same CDS sequence)	NM_001130543.1
Accession Version	NM_001130543.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1056bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-27
Organism	Rattus norvegicus(Norway rat)
Product	ribonucleoside-diphosphate reductase subunit M2 B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473950.2. On or before Jul 28, 2008 this sequence version replaced XM_235367.4, XM_001061249.1. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGGCGACC CGGAAAGGCC GGAAGCGGCC AGGCCGGAGG AGGGTGAGCA ATTGTGTCCA 
GAGACAAAAG AAAACGAGGT GAGGTCAAAT GAAGAGCCAC TCCTCAGAAA GAGTTCCCGA 
CGGTTTGTCA TCTTTCCAAT CCAGTACCCT GATATTTGGA AGATGTATAA GCAAGCACAG 
GCATCTTTCT GGACAGCAGA GGAGGTTGAC TTGTCCAAGG ACCTCCCTCA TTGGAACAAG 
CTTAAGTCGG AAGAGAAGTA TTTTATCTCC CACATCTTAG CATTTTTCGC AGCCAGCGAT 
GGAATTGTAA ATGAAAACTT GGTGGAGCGT TTTAGTCAAG AGGTGCAGGT TCCAGAGGCT 
CGCTGCTTCT ATGGCTTTCA GATTCTCATC GAGAATGTTC ACTCAGAGAT GTACAGTTTA 
CTAATAGACA CTTACATCAG AGATCCAAAG AAGAGGGAAT TTTTATTTAA TGCAATTGAA 
ACAATGCCGT ATGTTAAGAA AAAAGCAGAT TGGGCTCTGC GCTGGATAGC AGATAGGAAA 
TCTACTTTTG GGGAAAGAGT GGTGGCCTTC GCTGCTGTAG AAGGGATTTT CTTCTCTGGA 
TCATTTGCTG CTATATTCTG GCTAAAGAAG AGAGGCCTCA TGCCTGGGCT GACTTTTTCA 
AATGAACTGA TCAGCAGGGA TGAAGGACTT CACTGTGACT TTGCCTGCCT GATGTTTCAG 
TACCTGGTAA ACAAGCCTTC AGAAGAAAGG GTCATGGAAA TCATTGTTGA TGCTGTTAAA 
ATTGAGCAGG AGTTTTTAAC AGAAGCCTTG CCTGTTGGCC TCATTGGAAT GAACTGTGTT 
TTGATGAAAC AATATATTGA GTTTGTAGCT GACAGATTAC TTGGAGAACT TGGATTCTCA 
AAGATTTTTC AGGCAGAAAA TCCCTTTGAT TTTATGGAGA ACATTTCGTT AGAAGGGAAA 
ACAAATTTCT TTGAGAAACG AGTTTCTGAG TATCAGCGAT TTGCAGTCAT GGCAGAAACC 
ACAGATAATG TCTTCACCTT GGATGCAGAT TTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001124015.1
CDS	132..1187
Translation	MGDPERPEAARPEEGEQLCPETKENEVRSNEEPLLRKSSRRFVIFPIQYPDIWKMYKQAQASFWTAEEVDLSKDLPHWNKLKSEEKYFISHILAFFAASDGIVNENLVERFSQEVQVPEARCFYGFQILIENVHSEMYSLLIDTYIRDPKKREFLFNAIETMPYVKKKADWALRWIADRKSTFGERVVAFAAVEGIFFSGSFAAIFWLKKRGLMPGLTFSNELISRDEGLHCDFACLMFQYLVNKPSEERVMEIIVDAVKIEQEFLTEALPVGLIGMNCVLMKQYIEFVADRLLGELGFSKIFQAENPFDFMENISLEGKTNFFEKRVSEYQRFAVMAETTDNVFTLDADF

Target ORF information:

RefSeq Version	NM_001130543.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ribonucleotide reductase regulatory TP53 inducible subunit M2B (Rrm2b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001130543.1

ORF Insert Sequence:

ATGGGCGACC CGGAAAGGCC GGAAGCGGCC AGGCCGGAGG AGGGTGAGCA ATTGTGTCCA 
GAGACAAAAG AAAACGAGGT GAGGTCAAAT GAAGAGCCAC TCCTCAGAAA GAGTTCCCGA 
CGGTTTGTCA TCTTTCCAAT CCAGTACCCT GATATTTGGA AGATGTATAA GCAAGCACAG 
GCATCTTTCT GGACAGCAGA GGAGGTTGAC TTGTCCAAGG ACCTCCCTCA TTGGAACAAG 
CTTAAGTCGG AAGAGAAGTA TTTTATCTCC CACATCTTAG CATTTTTCGC AGCCAGCGAT 
GGAATTGTAA ATGAAAACTT GGTGGAGCGT TTTAGTCAAG AGGTGCAGGT TCCAGAGGCT 
CGCTGCTTCT ATGGCTTTCA GATTCTCATC GAGAATGTTC ACTCAGAGAT GTACAGTTTA 
CTAATAGACA CTTACATCAG AGATCCAAAG AAGAGGGAAT TTTTATTTAA TGCAATTGAA 
ACAATGCCGT ATGTTAAGAA AAAAGCAGAT TGGGCTCTGC GCTGGATAGC AGATAGGAAA 
TCTACTTTTG GGGAAAGAGT GGTGGCCTTC GCTGCTGTAG AAGGGATTTT CTTCTCTGGA 
TCATTTGCTG CTATATTCTG GCTAAAGAAG AGAGGCCTCA TGCCTGGGCT GACTTTTTCA 
AATGAACTGA TCAGCAGGGA TGAAGGACTT CACTGTGACT TTGCCTGCCT GATGTTTCAG 
TACCTGGTAA ACAAGCCTTC AGAAGAAAGG GTCATGGAAA TCATTGTTGA TGCTGTTAAA 
ATTGAGCAGG AGTTTTTAAC AGAAGCCTTG CCTGTTGGCC TCATTGGAAT GAACTGTGTT 
TTGATGAAAC AATATATTGA GTTTGTAGCT GACAGATTAC TTGGAGAACT TGGATTCTCA 
AAGATTTTTC AGGCAGAAAA TCCCTTTGAT TTTATGGAGA ACATTTCGTT AGAAGGGAAA 
ACAAATTTCT TTGAGAAACG AGTTTCTGAG TATCAGCGAT TTGCAGTCAT GGCAGAAACC 
ACAGATAATG TCTTCACCTT GGATGCAGAT TTCTAA