Tle2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tle2
Entrez Gene ID	299636
Full Name	transducin-like enhancer of split 2
General protein information	Preferred Names transducin-like enhancer of split 2 Names transducin-like enhancer protein 2 transducin-like enhancer of split 2 (E(sp1) homolog, Drosophila) transducin-like enhancer of split 2 homolog transducin-like enhancer of split 2, homolog of Drosophila E(spl)
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 7 Map Location: 7q11

mRNA and Protein(s)

mRNA	Protein	Name
XM_006240893.2	XP_006240955.1	transducin-like enhancer protein 2 isoform X2
XM_006240894.2	XP_006240956.1	transducin-like enhancer protein 2 isoform X3
NM_001039013.1	NP_001034102.1	transducin-like enhancer protein 2
XM_006240889.2	XP_006240951.1	transducin-like enhancer protein 2 isoform X1
XM_017594719.1	XP_017450208.1	transducin-like enhancer protein 2 isoform X4
XM_006240892.2	XP_006240954.1	transducin-like enhancer protein 2 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	TLE2	NP_003251.2
Canis lupus familiaris (dog)	TLE2	XP_005633143.1
Pan troglodytes (chimpanzee)	TLE2	XP_512979.3
Bos taurus (cattle)	TLE2	XP_005209071.1
Mus musculus (house mouse)	Tle2	NP_062699.1
Rattus norvegicus (Norway rat)	Tle2	NP_001034102.1

Gene Ontology
Bibliography

Related articles in PubMed

Comparison of tear protein levels in breast cancer patients and healthy controls using a de novo proteomic approach.
B?hm D, Keller K, Pieter J, Boehm N, Wolters D, Siggelkow W, Lebrecht A, Schmidt M, K?lbl H, Pfeiffer N, Grus FH
Oncology reports28(2)429-38(2012 Aug)

Groucho binds two conserved regions of LEF-1 for HDAC-dependent repression.
Arce L, Pate KT, Waterman ML
BMC cancer9159(2009 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Tle2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tle2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa13757	NM_001039013.1 Latest version!	Rattus norvegicus TLE family member 2, transcriptional corepressor (Tle2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
ORa29836	XM_006240889.2 Latest version!	Rattus norvegicus transducin-like enhancer of split 2 (Tle2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa29836	XM_006240892.2 Latest version!	Rattus norvegicus transducin-like enhancer of split 2 (Tle2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa29837	XM_006240893.2 Latest version!	Rattus norvegicus transducin-like enhancer of split 2 (Tle2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa29838	XM_006240894.2 Latest version!	Rattus norvegicus transducin-like enhancer of split 2 (Tle2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa61927	XM_017594719.1 Latest version!	Rattus norvegicus transducin-like enhancer of split 2 (Tle2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tle2 ( NM_001039013.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001034102.1 Rattus norvegicus TLE family member 2, transcriptional corepressor (Tle2), mRNA.

Price & Availability↑

CloneID	ORa13757
Clone ID Related Accession (Same CDS sequence)	NM_001039013.1
Accession Version	NM_001039013.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2091bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-12
Organism	Rattus norvegicus(Norway rat)
Product	transducin-like enhancer protein 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC100652.1. On Jan 12, 2006 this sequence version replaced XM_216845.3. ##Evidence-Data-START## Transcript exon combination :: BC100652.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGTACCCCC AGGGAAGGCA CCCGACCCCG CTGCAGTCTG GCCAGCCTTT CAAGTTCTCA 
GTCCTGGAAA TCTGTGACCG GATCAAAGAG GAATTTCAGT TTCTCCAAGC TCAATACCAC 
AGCCTTAAGC TAGAGTGTGA GAAGCTGGCC AGCGAGAAGA CAGAAATGCA AAGGCATTAT 
GTGATGTACT ATGAGATGTC CTACGGACTC AACATTGAGA TGCACAAACA GGCTGAGATC 
GTGAAGCGCC TCAGTGCAAT CTGTGCCCAG ATGGTCCCAT TCCTCACTCA GGAGCATCAG 
CAGCAGGTGC TCCAGGCTGT GGACCGAGCC AAGCAGGTGA CCGTGGGGGA ACTGAACAAC 
CTCTTGGGGC AGCAGAATCA GCTCCAGCCG CTGTCCCATG CACCCCCTGT GCCTCTCACC 
CCACGCCCAG CTGGCCTGGT GGGTGCCGGG GCCACTGGGC TGCTGGCCCT CTCTGGGGCG 
CTGGCTGCGC AGGCCCAGCT GGTGGCTGCT GTTAAGGAAG ACCGTGTGGG TGTGGACACC 
GAGGGGTCCA GAGTGGACAG AGCTGCCAGC AGGAGTTCAT CCCCCTCACC CCCTGAGAGT 
CTGGCGGAAG AGGAGCATCC TAGCAGCCGA GGCAGTAACG GGAAGCAGCA GAGAGCCGAA 
GACAAGAATA TGTCAGGGCC TTATGACAGT GAGGAAGACA AGAGTGACTA TAACCTGGTA 
GTGGATGAGG ACCAACCGTC AGAGCCCCCC AGCCCTGCGA CCACCCCCTG TGGGAAGGCG 
CCCCTCTGCA TCCCTGCCCG CAGGGACCTC ACGGACAGTC CAGCCTCCCT GGCTTCCAGC 
TTGGGCTCAC CACTCCCCAG AAGCAAAGAC CTAGCCCTGA ACGATCTCTC CACAGGTACT 
CCCGCCTCTG GGTCATGTGG TCCCTCTCCA CCCCAGGACT CGTCCACCCC AGGGCCCAGC 
TCAGCCAGTC ACCTCTGCCA GCTGGCTACT CAGCCGGCAC CGCCCACAGA CAGCATTGCC 
CTGAGGAGTC CCCTGACCCT GTCCAGCCCT TTCACCTCGT CCTTCAGCCT GGGCTCCCAC 
AGCACCCTCA ATGGGGACCT CTCCATGCCT GGCTCCTATG TCAGCCTCCA CCTGTCCCCC 
CAGATGCAAC CGGTGCCCTT CCCATCGGAT GCACTGGTAG GCACTGGCAT CCCTCGCCAC 
GCGAGGCAGC TGCACACACT GGCCCATGGT GAGGTGGTGT GTGCCGTCAC CATCAGCAGC 
TCCACGCAGC ACGTGTACAC TGGCGGCAAA GGCTGCGTGA AGGTTTGGGA CGTGGGCCAG 
CCGGGCAGCA AGACACCTGT GGCACAGCTG GATTGCCTGA ACCGGGACAA CTACATTCGC 
TCCTGCAAGC TGCTGCCTGA CGGGCAGAGC CTGATCGTGG GTGGCGAGGC CAGTACCCTG 
TCCATTTGGG ACCTGGCAGC ACCCACACCA CGCATCAAAG CAGAGCTGAC CTCGTCCGCC 
CCCGCCTGTT ACGCGCTGGC CATCAGCCCG GATGCCAAGG TCTGCTTCTC CTGCTGCAGC 
GACGGTAACA TCGTGGTCTG GGACCTGCAG AACCAGGCCA TGGTCAGACA GTTCCAGGGC 
CACACGGACG GGGCCAGCTG CATTGACATA TCAGACTACG GGACCCGGCT GTGGACTGGG 
GGCCTGGACA ACACCGTGCG CTGCTGGGAT CTGCGCGAGG GCCGCCAGCT GCAGCAACAT 
GACTTCAGTT CCCAGATCTT CTCGTTGGGC CACTGTCCCA GTCAGGACTG GCTGGCCGTG 
GGGATGGAAA GCAGCCACGT GGAGGTCCTG CATGTGCGCA AGCCTGAGAA GTACCAGCTC 
CGCCTGCATG AGAGCTGCGT GCTGTCGCTC AAGTTCGCTT CCTGCGGACG CTGGTTTGTG 
AGCACAGGCA AGGATAACCT GCTCAATGCC TGGAGGACAC CCTATGGGGC CAGCATTTTC 
CAGTCCAAAG AGTCTTCTTC TGTGTTGAGC TGCGACATCT CCAGGAACAA CAAGTACATT 
GTGACAGGCT CAGGGGACAA GAAGGCCACT GTGTATGAGG TGGTGTACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001034102.1
CDS	178..2268
Translation	MYPQGRHPTPLQSGQPFKFSVLEICDRIKEEFQFLQAQYHSLKLECEKLASEKTEMQRHYVMYYEMSYGLNIEMHKQAEIVKRLSAICAQMVPFLTQEHQQQVLQAVDRAKQVTVGELNNLLGQQNQLQPLSHAPPVPLTPRPAGLVGAGATGLLALSGALAAQAQLVAAVKEDRVGVDTEGSRVDRAASRSSSPSPPESLAEEEHPSSRGSNGKQQRAEDKNMSGPYDSEEDKSDYNLVVDEDQPSEPPSPATTPCGKAPLCIPARRDLTDSPASLASSLGSPLPRSKDLALNDLSTGTPASGSCGPSPPQDSSTPGPSSASHLCQLATQPAPPTDSIALRSPLTLSSPFTSSFSLGSHSTLNGDLSMPGSYVSLHLSPQMQPVPFPSDALVGTGIPRHARQLHTLAHGEVVCAVTISSSTQHVYTGGKGCVKVWDVGQPGSKTPVAQLDCLNRDNYIRSCKLLPDGQSLIVGGEASTLSIWDLAAPTPRIKAELTSSAPACYALAISPDAKVCFSCCSDGNIVVWDLQNQAMVRQFQGHTDGASCIDISDYGTRLWTGGLDNTVRCWDLREGRQLQQHDFSSQIFSLGHCPSQDWLAVGMESSHVEVLHVRKPEKYQLRLHESCVLSLKFASCGRWFVSTGKDNLLNAWRTPYGASIFQSKESSSVLSCDISRNNKYIVTGSGDKKATVYEVVY

Target ORF information:

RefSeq Version	NM_001039013.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus TLE family member 2, transcriptional corepressor (Tle2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001039013.1

ORF Insert Sequence:

ATGTACCCCC AGGGAAGGCA CCCGACCCCG CTGCAGTCTG GCCAGCCTTT CAAGTTCTCA 
GTCCTGGAAA TCTGTGACCG GATCAAAGAG GAATTTCAGT TTCTCCAAGC TCAATACCAC 
AGCCTTAAGC TAGAGTGTGA GAAGCTGGCC AGCGAGAAGA CAGAAATGCA AAGGCATTAT 
GTGATGTACT ATGAGATGTC CTACGGACTC AACATTGAGA TGCACAAACA GGCTGAGATC 
GTGAAGCGCC TCAGTGCAAT CTGTGCCCAG ATGGTCCCAT TCCTCACTCA GGAGCATCAG 
CAGCAGGTGC TCCAGGCTGT GGACCGAGCC AAGCAGGTGA CCGTGGGGGA ACTGAACAAC 
CTCTTGGGGC AGCAGAATCA GCTCCAGCCG CTGTCCCATG CACCCCCTGT GCCTCTCACC 
CCACGCCCAG CTGGCCTGGT GGGTGCCGGG GCCACTGGGC TGCTGGCCCT CTCTGGGGCG 
CTGGCTGCGC AGGCCCAGCT GGTGGCTGCT GTTAAGGAAG ACCGTGTGGG TGTGGACACC 
GAGGGGTCCA GAGTGGACAG AGCTGCCAGC AGGAGTTCAT CCCCCTCACC CCCTGAGAGT 
CTGGCGGAAG AGGAGCATCC TAGCAGCCGA GGCAGTAACG GGAAGCAGCA GAGAGCCGAA 
GACAAGAATA TGTCAGGGCC TTATGACAGT GAGGAAGACA AGAGTGACTA TAACCTGGTA 
GTGGATGAGG ACCAACCGTC AGAGCCCCCC AGCCCTGCGA CCACCCCCTG TGGGAAGGCG 
CCCCTCTGCA TCCCTGCCCG CAGGGACCTC ACGGACAGTC CAGCCTCCCT GGCTTCCAGC 
TTGGGCTCAC CACTCCCCAG AAGCAAAGAC CTAGCCCTGA ACGATCTCTC CACAGGTACT 
CCCGCCTCTG GGTCATGTGG TCCCTCTCCA CCCCAGGACT CGTCCACCCC AGGGCCCAGC 
TCAGCCAGTC ACCTCTGCCA GCTGGCTACT CAGCCGGCAC CGCCCACAGA CAGCATTGCC 
CTGAGGAGTC CCCTGACCCT GTCCAGCCCT TTCACCTCGT CCTTCAGCCT GGGCTCCCAC 
AGCACCCTCA ATGGGGACCT CTCCATGCCT GGCTCCTATG TCAGCCTCCA CCTGTCCCCC 
CAGATGCAAC CGGTGCCCTT CCCATCGGAT GCACTGGTAG GCACTGGCAT CCCTCGCCAC 
GCGAGGCAGC TGCACACACT GGCCCATGGT GAGGTGGTGT GTGCCGTCAC CATCAGCAGC 
TCCACGCAGC ACGTGTACAC TGGCGGCAAA GGCTGCGTGA AGGTTTGGGA CGTGGGCCAG 
CCGGGCAGCA AGACACCTGT GGCACAGCTG GATTGCCTGA ACCGGGACAA CTACATTCGC 
TCCTGCAAGC TGCTGCCTGA CGGGCAGAGC CTGATCGTGG GTGGCGAGGC CAGTACCCTG 
TCCATTTGGG ACCTGGCAGC ACCCACACCA CGCATCAAAG CAGAGCTGAC CTCGTCCGCC 
CCCGCCTGTT ACGCGCTGGC CATCAGCCCG GATGCCAAGG TCTGCTTCTC CTGCTGCAGC 
GACGGTAACA TCGTGGTCTG GGACCTGCAG AACCAGGCCA TGGTCAGACA GTTCCAGGGC 
CACACGGACG GGGCCAGCTG CATTGACATA TCAGACTACG GGACCCGGCT GTGGACTGGG 
GGCCTGGACA ACACCGTGCG CTGCTGGGAT CTGCGCGAGG GCCGCCAGCT GCAGCAACAT 
GACTTCAGTT CCCAGATCTT CTCGTTGGGC CACTGTCCCA GTCAGGACTG GCTGGCCGTG 
GGGATGGAAA GCAGCCACGT GGAGGTCCTG CATGTGCGCA AGCCTGAGAA GTACCAGCTC 
CGCCTGCATG AGAGCTGCGT GCTGTCGCTC AAGTTCGCTT CCTGCGGACG CTGGTTTGTG 
AGCACAGGCA AGGATAACCT GCTCAATGCC TGGAGGACAC CCTATGGGGC CAGCATTTTC 
CAGTCCAAAG AGTCTTCTTC TGTGTTGAGC TGCGACATCT CCAGGAACAA CAAGTACATT 
GTGACAGGCT CAGGGGACAA GAAGGCCACT GTGTATGAGG TGGTGTACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tle2 ( XM_006240889.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006240951.1 Rattus norvegicus transducin-like enhancer of split 2 (Tle2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa29836
Clone ID Related Accession (Same CDS sequence)	XM_006240889.2 , XM_006240892.2
Accession Version	XM_006240889.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2232bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	transducin-like enhancer protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006240889.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACCCCC AGGGAAGGCA CCCGACCCCG CTGCAGTCTG GCCAACCTTT CAAGTTCTCA 
GTCCTGGAAA TCTGTGACCG GATCAAAGAG GAATTTCAGT TTCTCCAAGC TCAATACCAC 
AGCCTTAAGC TAGAGTGTGA GAAGCTGGCC AGCGAGAAGA CAGAAATGCA AAGGCATTAT 
GTGATGTACT ATGAGATGTC CTACGGACTC AACATTGAGA TGCACAAACA GGCTGAGATC 
GTGAAGCGCC TCAGTGCAAT CTGTGCCCAG ATGGTCCCAT TCCTCACTCA GGAGCATCAG 
CAGCAGGTGC TCCAGGCTGT GGACCGAGCC AAGCAGGTGA CCGTGGGGGA ACTGAACAAC 
CTCTTGGGGC AGCAGAATCA GCTCCAGCCG CTGTCCCATG CACCCCCTGT GCCTCTCACC 
CCACGCCCAG CTGGCCTGGT GGGTGCCGGG GCCACTGGGC TGCTGGCCCT CTCTGGGGCG 
CTGGCTGCGC AGGCCCAGCT GGTGGCTGCT GTTAAGGAAG ACCGTGTGGG TGTGGACACC 
GAGGGGTCCA GAGTGGACAG AGCTGCCAGC AGGAGTTCAT CCCCCTCACC CCCTGAGAGT 
CTGGCAGAAG AGGAGCATCC TAGCAGCCGA GACAGTAACG GGAAGCAGCA GAGAGCCGAA 
GACAAGAATA TGTCAGGGCC TTATGACAGT GAGGAAGACA AGAGTGACTA TAACCTGGTA 
GTGGATGAGG ACCAACCGTC AGAGCCCCCC AGCCCTGCGA CCACCCCCTG TGGGAAGGCG 
CCCCTCTGCA TCCCTGCCCG CAGGGACCTC ACGGACAGTC CAGCCTCCCT GGCTTCCAGC 
TTGGGCTCAC CACTCCCCAG AAGCAAAGAC CTAGCCCTGA ACGATCTCTC CACAGGTACT 
CCCGCCTCTG GGTCATGTGG TCCCTCTCCA CCCCAGGACT CGTCCACCCC AGGGCCCAGC 
TCAGCCAGTC ACCTCTGCCA GCTGGCTACT CAGCCGGCAC CGCCCACAGA CAGCATTGCC 
CTGAGGAGTC CCCTGACCCT GTCCAGCCCT TTCACCTCGT CCTTCAGCCT GGGCTCCCAC 
AGCACCCTCA ATGGGGACCT CTCCATGCCT GGCTCCTATG TCAGCCTCCA CCTGTCCCCC 
CAGGTCAGCA GCTCTGTGGT GTACGGATGC TCACCTCTGA TGGCATTTGA GTCGCACCCC 
CATCTCCGAG GCTCGTCCAT CTCCTTGCCT AGCATCCCGG GGGCTAAGCC GGCTTACTCC 
TTCCACGTGT CTGCGGATGG ACAGATGCAA CCGGTGCCCT TCCCATCGGA TGCACTGGTA 
GGCACTGGCA TCCCTCGCCA CGCGAGGCAG CTGCACACAC TGGCCCATGG TGAGGTGGTG 
TGTGCCGTCA CCATCAGCAG CTCCACGCAG CACGTGTACA CGGGCGGCAA AGGCTGCGTG 
AAGGTTTGGG ACGTGGGCCA GCCGGGCAGC AAGACACCTG TGGCACAGCT GGATTGCCTG 
AACCGGGACA ACTACATTCG CTCCTGCAAG CTGCTGCCTG ACGGGCAGAG CCTGATCGTG 
GGTGGCGAGG CCAGTACCCT GTCCATTTGG GACCTGGCAG CACCCACACC ACGCATCAAA 
GCAGAGCTGA CCTCGTCCGC CCCCGCCTGT TACGCGCTGG CCATCAGCCC GGATGCCAAG 
GTCTGCTTCT CCTGCTGCAG CGACGGTAAC ATCGTGGTCT GGGACCTGCA GAACCAGGCC 
ATGGTCAGAC AGTTCCAGGG CCACACGGAC GGGGCCAGCT GCATTGACAT ATCAGACTAC 
GGGACCCGGC TGTGGACTGG GGGCCTGGAC AACACCGTGC GCTGCTGGGA TCTGCGCGAG 
GGCCGCCAGC TGCAGCAACA TGACTTCAGT TCCCAGATCT TCTCGTTGGG CCACTGTCCC 
AGTCAGGACT GGCTGGCCGT GGGGATGGAA AGCAGCCACG TGGAGGTCCT GCATGTGCGC 
AAGCCTGAGA AGTACCAGCT CCGCCTGCAC GAGAGCTGCG TGCTGTCGCT CAAGTTCGCT 
TCCTGCGGAC GCTGGTTTGT GAGCACAGGC AAGGATAACC TGCTCAATGC CTGGAGGACA 
CCCTATGGGG CCAGCATTTT CCAGTCCAAA GAGTCTTCTT CCGTGTTGAG CTGCGACATC 
TCCAGGAACA ACAAGTACAT TGTGACAGGC TCAGGGGACA AGAAGGCCAC TGTGTATGAG 
GTGGTGTACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006240951.1
CDS	350..2581
Translation	MYPQGRHPTPLQSGQPFKFSVLEICDRIKEEFQFLQAQYHSLKLECEKLASEKTEMQRHYVMYYEMSYGLNIEMHKQAEIVKRLSAICAQMVPFLTQEHQQQVLQAVDRAKQVTVGELNNLLGQQNQLQPLSHAPPVPLTPRPAGLVGAGATGLLALSGALAAQAQLVAAVKEDRVGVDTEGSRVDRAASRSSSPSPPESLAEEEHPSSRDSNGKQQRAEDKNMSGPYDSEEDKSDYNLVVDEDQPSEPPSPATTPCGKAPLCIPARRDLTDSPASLASSLGSPLPRSKDLALNDLSTGTPASGSCGPSPPQDSSTPGPSSASHLCQLATQPAPPTDSIALRSPLTLSSPFTSSFSLGSHSTLNGDLSMPGSYVSLHLSPQVSSSVVYGCSPLMAFESHPHLRGSSISLPSIPGAKPAYSFHVSADGQMQPVPFPSDALVGTGIPRHARQLHTLAHGEVVCAVTISSSTQHVYTGGKGCVKVWDVGQPGSKTPVAQLDCLNRDNYIRSCKLLPDGQSLIVGGEASTLSIWDLAAPTPRIKAELTSSAPACYALAISPDAKVCFSCCSDGNIVVWDLQNQAMVRQFQGHTDGASCIDISDYGTRLWTGGLDNTVRCWDLREGRQLQQHDFSSQIFSLGHCPSQDWLAVGMESSHVEVLHVRKPEKYQLRLHESCVLSLKFASCGRWFVSTGKDNLLNAWRTPYGASIFQSKESSSVLSCDISRNNKYIVTGSGDKKATVYEVVY

Target ORF information:

RefSeq Version	XM_006240889.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus transducin-like enhancer of split 2 (Tle2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006240889.2

ORF Insert Sequence:

ATGTACCCCC AGGGAAGGCA CCCGACCCCG CTGCAGTCTG GCCAACCTTT CAAGTTCTCA 
GTCCTGGAAA TCTGTGACCG GATCAAAGAG GAATTTCAGT TTCTCCAAGC TCAATACCAC 
AGCCTTAAGC TAGAGTGTGA GAAGCTGGCC AGCGAGAAGA CAGAAATGCA AAGGCATTAT 
GTGATGTACT ATGAGATGTC CTACGGACTC AACATTGAGA TGCACAAACA GGCTGAGATC 
GTGAAGCGCC TCAGTGCAAT CTGTGCCCAG ATGGTCCCAT TCCTCACTCA GGAGCATCAG 
CAGCAGGTGC TCCAGGCTGT GGACCGAGCC AAGCAGGTGA CCGTGGGGGA ACTGAACAAC 
CTCTTGGGGC AGCAGAATCA GCTCCAGCCG CTGTCCCATG CACCCCCTGT GCCTCTCACC 
CCACGCCCAG CTGGCCTGGT GGGTGCCGGG GCCACTGGGC TGCTGGCCCT CTCTGGGGCG 
CTGGCTGCGC AGGCCCAGCT GGTGGCTGCT GTTAAGGAAG ACCGTGTGGG TGTGGACACC 
GAGGGGTCCA GAGTGGACAG AGCTGCCAGC AGGAGTTCAT CCCCCTCACC CCCTGAGAGT 
CTGGCAGAAG AGGAGCATCC TAGCAGCCGA GACAGTAACG GGAAGCAGCA GAGAGCCGAA 
GACAAGAATA TGTCAGGGCC TTATGACAGT GAGGAAGACA AGAGTGACTA TAACCTGGTA 
GTGGATGAGG ACCAACCGTC AGAGCCCCCC AGCCCTGCGA CCACCCCCTG TGGGAAGGCG 
CCCCTCTGCA TCCCTGCCCG CAGGGACCTC ACGGACAGTC CAGCCTCCCT GGCTTCCAGC 
TTGGGCTCAC CACTCCCCAG AAGCAAAGAC CTAGCCCTGA ACGATCTCTC CACAGGTACT 
CCCGCCTCTG GGTCATGTGG TCCCTCTCCA CCCCAGGACT CGTCCACCCC AGGGCCCAGC 
TCAGCCAGTC ACCTCTGCCA GCTGGCTACT CAGCCGGCAC CGCCCACAGA CAGCATTGCC 
CTGAGGAGTC CCCTGACCCT GTCCAGCCCT TTCACCTCGT CCTTCAGCCT GGGCTCCCAC 
AGCACCCTCA ATGGGGACCT CTCCATGCCT GGCTCCTATG TCAGCCTCCA CCTGTCCCCC 
CAGGTCAGCA GCTCTGTGGT GTACGGATGC TCACCTCTGA TGGCATTTGA GTCGCACCCC 
CATCTCCGAG GCTCGTCCAT CTCCTTGCCT AGCATCCCGG GGGCTAAGCC GGCTTACTCC 
TTCCACGTGT CTGCGGATGG ACAGATGCAA CCGGTGCCCT TCCCATCGGA TGCACTGGTA 
GGCACTGGCA TCCCTCGCCA CGCGAGGCAG CTGCACACAC TGGCCCATGG TGAGGTGGTG 
TGTGCCGTCA CCATCAGCAG CTCCACGCAG CACGTGTACA CGGGCGGCAA AGGCTGCGTG 
AAGGTTTGGG ACGTGGGCCA GCCGGGCAGC AAGACACCTG TGGCACAGCT GGATTGCCTG 
AACCGGGACA ACTACATTCG CTCCTGCAAG CTGCTGCCTG ACGGGCAGAG CCTGATCGTG 
GGTGGCGAGG CCAGTACCCT GTCCATTTGG GACCTGGCAG CACCCACACC ACGCATCAAA 
GCAGAGCTGA CCTCGTCCGC CCCCGCCTGT TACGCGCTGG CCATCAGCCC GGATGCCAAG 
GTCTGCTTCT CCTGCTGCAG CGACGGTAAC ATCGTGGTCT GGGACCTGCA GAACCAGGCC 
ATGGTCAGAC AGTTCCAGGG CCACACGGAC GGGGCCAGCT GCATTGACAT ATCAGACTAC 
GGGACCCGGC TGTGGACTGG GGGCCTGGAC AACACCGTGC GCTGCTGGGA TCTGCGCGAG 
GGCCGCCAGC TGCAGCAACA TGACTTCAGT TCCCAGATCT TCTCGTTGGG CCACTGTCCC 
AGTCAGGACT GGCTGGCCGT GGGGATGGAA AGCAGCCACG TGGAGGTCCT GCATGTGCGC 
AAGCCTGAGA AGTACCAGCT CCGCCTGCAC GAGAGCTGCG TGCTGTCGCT CAAGTTCGCT 
TCCTGCGGAC GCTGGTTTGT GAGCACAGGC AAGGATAACC TGCTCAATGC CTGGAGGACA 
CCCTATGGGG CCAGCATTTT CCAGTCCAAA GAGTCTTCTT CCGTGTTGAG CTGCGACATC 
TCCAGGAACA ACAAGTACAT TGTGACAGGC TCAGGGGACA AGAAGGCCAC TGTGTATGAG 
GTGGTGTACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tle2 ( XM_006240892.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006240954.1 Rattus norvegicus transducin-like enhancer of split 2 (Tle2), transcript variant X2, mRNA.

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CloneID	ORa29836
Clone ID Related Accession (Same CDS sequence)	XM_006240889.2 , XM_006240892.2
Accession Version	XM_006240892.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2232bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	transducin-like enhancer protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006240892.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACCCCC AGGGAAGGCA CCCGACCCCG CTGCAGTCTG GCCAACCTTT CAAGTTCTCA 
GTCCTGGAAA TCTGTGACCG GATCAAAGAG GAATTTCAGT TTCTCCAAGC TCAATACCAC 
AGCCTTAAGC TAGAGTGTGA GAAGCTGGCC AGCGAGAAGA CAGAAATGCA AAGGCATTAT 
GTGATGTACT ATGAGATGTC CTACGGACTC AACATTGAGA TGCACAAACA GGCTGAGATC 
GTGAAGCGCC TCAGTGCAAT CTGTGCCCAG ATGGTCCCAT TCCTCACTCA GGAGCATCAG 
CAGCAGGTGC TCCAGGCTGT GGACCGAGCC AAGCAGGTGA CCGTGGGGGA ACTGAACAAC 
CTCTTGGGGC AGCAGAATCA GCTCCAGCCG CTGTCCCATG CACCCCCTGT GCCTCTCACC 
CCACGCCCAG CTGGCCTGGT GGGTGCCGGG GCCACTGGGC TGCTGGCCCT CTCTGGGGCG 
CTGGCTGCGC AGGCCCAGCT GGTGGCTGCT GTTAAGGAAG ACCGTGTGGG TGTGGACACC 
GAGGGGTCCA GAGTGGACAG AGCTGCCAGC AGGAGTTCAT CCCCCTCACC CCCTGAGAGT 
CTGGCAGAAG AGGAGCATCC TAGCAGCCGA GACAGTAACG GGAAGCAGCA GAGAGCCGAA 
GACAAGAATA TGTCAGGGCC TTATGACAGT GAGGAAGACA AGAGTGACTA TAACCTGGTA 
GTGGATGAGG ACCAACCGTC AGAGCCCCCC AGCCCTGCGA CCACCCCCTG TGGGAAGGCG 
CCCCTCTGCA TCCCTGCCCG CAGGGACCTC ACGGACAGTC CAGCCTCCCT GGCTTCCAGC 
TTGGGCTCAC CACTCCCCAG AAGCAAAGAC CTAGCCCTGA ACGATCTCTC CACAGGTACT 
CCCGCCTCTG GGTCATGTGG TCCCTCTCCA CCCCAGGACT CGTCCACCCC AGGGCCCAGC 
TCAGCCAGTC ACCTCTGCCA GCTGGCTACT CAGCCGGCAC CGCCCACAGA CAGCATTGCC 
CTGAGGAGTC CCCTGACCCT GTCCAGCCCT TTCACCTCGT CCTTCAGCCT GGGCTCCCAC 
AGCACCCTCA ATGGGGACCT CTCCATGCCT GGCTCCTATG TCAGCCTCCA CCTGTCCCCC 
CAGGTCAGCA GCTCTGTGGT GTACGGATGC TCACCTCTGA TGGCATTTGA GTCGCACCCC 
CATCTCCGAG GCTCGTCCAT CTCCTTGCCT AGCATCCCGG GGGCTAAGCC GGCTTACTCC 
TTCCACGTGT CTGCGGATGG ACAGATGCAA CCGGTGCCCT TCCCATCGGA TGCACTGGTA 
GGCACTGGCA TCCCTCGCCA CGCGAGGCAG CTGCACACAC TGGCCCATGG TGAGGTGGTG 
TGTGCCGTCA CCATCAGCAG CTCCACGCAG CACGTGTACA CGGGCGGCAA AGGCTGCGTG 
AAGGTTTGGG ACGTGGGCCA GCCGGGCAGC AAGACACCTG TGGCACAGCT GGATTGCCTG 
AACCGGGACA ACTACATTCG CTCCTGCAAG CTGCTGCCTG ACGGGCAGAG CCTGATCGTG 
GGTGGCGAGG CCAGTACCCT GTCCATTTGG GACCTGGCAG CACCCACACC ACGCATCAAA 
GCAGAGCTGA CCTCGTCCGC CCCCGCCTGT TACGCGCTGG CCATCAGCCC GGATGCCAAG 
GTCTGCTTCT CCTGCTGCAG CGACGGTAAC ATCGTGGTCT GGGACCTGCA GAACCAGGCC 
ATGGTCAGAC AGTTCCAGGG CCACACGGAC GGGGCCAGCT GCATTGACAT ATCAGACTAC 
GGGACCCGGC TGTGGACTGG GGGCCTGGAC AACACCGTGC GCTGCTGGGA TCTGCGCGAG 
GGCCGCCAGC TGCAGCAACA TGACTTCAGT TCCCAGATCT TCTCGTTGGG CCACTGTCCC 
AGTCAGGACT GGCTGGCCGT GGGGATGGAA AGCAGCCACG TGGAGGTCCT GCATGTGCGC 
AAGCCTGAGA AGTACCAGCT CCGCCTGCAC GAGAGCTGCG TGCTGTCGCT CAAGTTCGCT 
TCCTGCGGAC GCTGGTTTGT GAGCACAGGC AAGGATAACC TGCTCAATGC CTGGAGGACA 
CCCTATGGGG CCAGCATTTT CCAGTCCAAA GAGTCTTCTT CCGTGTTGAG CTGCGACATC 
TCCAGGAACA ACAAGTACAT TGTGACAGGC TCAGGGGACA AGAAGGCCAC TGTGTATGAG 
GTGGTGTACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006240954.1
CDS	263..2494
Translation	MYPQGRHPTPLQSGQPFKFSVLEICDRIKEEFQFLQAQYHSLKLECEKLASEKTEMQRHYVMYYEMSYGLNIEMHKQAEIVKRLSAICAQMVPFLTQEHQQQVLQAVDRAKQVTVGELNNLLGQQNQLQPLSHAPPVPLTPRPAGLVGAGATGLLALSGALAAQAQLVAAVKEDRVGVDTEGSRVDRAASRSSSPSPPESLAEEEHPSSRDSNGKQQRAEDKNMSGPYDSEEDKSDYNLVVDEDQPSEPPSPATTPCGKAPLCIPARRDLTDSPASLASSLGSPLPRSKDLALNDLSTGTPASGSCGPSPPQDSSTPGPSSASHLCQLATQPAPPTDSIALRSPLTLSSPFTSSFSLGSHSTLNGDLSMPGSYVSLHLSPQVSSSVVYGCSPLMAFESHPHLRGSSISLPSIPGAKPAYSFHVSADGQMQPVPFPSDALVGTGIPRHARQLHTLAHGEVVCAVTISSSTQHVYTGGKGCVKVWDVGQPGSKTPVAQLDCLNRDNYIRSCKLLPDGQSLIVGGEASTLSIWDLAAPTPRIKAELTSSAPACYALAISPDAKVCFSCCSDGNIVVWDLQNQAMVRQFQGHTDGASCIDISDYGTRLWTGGLDNTVRCWDLREGRQLQQHDFSSQIFSLGHCPSQDWLAVGMESSHVEVLHVRKPEKYQLRLHESCVLSLKFASCGRWFVSTGKDNLLNAWRTPYGASIFQSKESSSVLSCDISRNNKYIVTGSGDKKATVYEVVY

Target ORF information:

RefSeq Version	XM_006240892.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus transducin-like enhancer of split 2 (Tle2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006240892.2

ORF Insert Sequence:

ATGTACCCCC AGGGAAGGCA CCCGACCCCG CTGCAGTCTG GCCAACCTTT CAAGTTCTCA 
GTCCTGGAAA TCTGTGACCG GATCAAAGAG GAATTTCAGT TTCTCCAAGC TCAATACCAC 
AGCCTTAAGC TAGAGTGTGA GAAGCTGGCC AGCGAGAAGA CAGAAATGCA AAGGCATTAT 
GTGATGTACT ATGAGATGTC CTACGGACTC AACATTGAGA TGCACAAACA GGCTGAGATC 
GTGAAGCGCC TCAGTGCAAT CTGTGCCCAG ATGGTCCCAT TCCTCACTCA GGAGCATCAG 
CAGCAGGTGC TCCAGGCTGT GGACCGAGCC AAGCAGGTGA CCGTGGGGGA ACTGAACAAC 
CTCTTGGGGC AGCAGAATCA GCTCCAGCCG CTGTCCCATG CACCCCCTGT GCCTCTCACC 
CCACGCCCAG CTGGCCTGGT GGGTGCCGGG GCCACTGGGC TGCTGGCCCT CTCTGGGGCG 
CTGGCTGCGC AGGCCCAGCT GGTGGCTGCT GTTAAGGAAG ACCGTGTGGG TGTGGACACC 
GAGGGGTCCA GAGTGGACAG AGCTGCCAGC AGGAGTTCAT CCCCCTCACC CCCTGAGAGT 
CTGGCAGAAG AGGAGCATCC TAGCAGCCGA GACAGTAACG GGAAGCAGCA GAGAGCCGAA 
GACAAGAATA TGTCAGGGCC TTATGACAGT GAGGAAGACA AGAGTGACTA TAACCTGGTA 
GTGGATGAGG ACCAACCGTC AGAGCCCCCC AGCCCTGCGA CCACCCCCTG TGGGAAGGCG 
CCCCTCTGCA TCCCTGCCCG CAGGGACCTC ACGGACAGTC CAGCCTCCCT GGCTTCCAGC 
TTGGGCTCAC CACTCCCCAG AAGCAAAGAC CTAGCCCTGA ACGATCTCTC CACAGGTACT 
CCCGCCTCTG GGTCATGTGG TCCCTCTCCA CCCCAGGACT CGTCCACCCC AGGGCCCAGC 
TCAGCCAGTC ACCTCTGCCA GCTGGCTACT CAGCCGGCAC CGCCCACAGA CAGCATTGCC 
CTGAGGAGTC CCCTGACCCT GTCCAGCCCT TTCACCTCGT CCTTCAGCCT GGGCTCCCAC 
AGCACCCTCA ATGGGGACCT CTCCATGCCT GGCTCCTATG TCAGCCTCCA CCTGTCCCCC 
CAGGTCAGCA GCTCTGTGGT GTACGGATGC TCACCTCTGA TGGCATTTGA GTCGCACCCC 
CATCTCCGAG GCTCGTCCAT CTCCTTGCCT AGCATCCCGG GGGCTAAGCC GGCTTACTCC 
TTCCACGTGT CTGCGGATGG ACAGATGCAA CCGGTGCCCT TCCCATCGGA TGCACTGGTA 
GGCACTGGCA TCCCTCGCCA CGCGAGGCAG CTGCACACAC TGGCCCATGG TGAGGTGGTG 
TGTGCCGTCA CCATCAGCAG CTCCACGCAG CACGTGTACA CGGGCGGCAA AGGCTGCGTG 
AAGGTTTGGG ACGTGGGCCA GCCGGGCAGC AAGACACCTG TGGCACAGCT GGATTGCCTG 
AACCGGGACA ACTACATTCG CTCCTGCAAG CTGCTGCCTG ACGGGCAGAG CCTGATCGTG 
GGTGGCGAGG CCAGTACCCT GTCCATTTGG GACCTGGCAG CACCCACACC ACGCATCAAA 
GCAGAGCTGA CCTCGTCCGC CCCCGCCTGT TACGCGCTGG CCATCAGCCC GGATGCCAAG 
GTCTGCTTCT CCTGCTGCAG CGACGGTAAC ATCGTGGTCT GGGACCTGCA GAACCAGGCC 
ATGGTCAGAC AGTTCCAGGG CCACACGGAC GGGGCCAGCT GCATTGACAT ATCAGACTAC 
GGGACCCGGC TGTGGACTGG GGGCCTGGAC AACACCGTGC GCTGCTGGGA TCTGCGCGAG 
GGCCGCCAGC TGCAGCAACA TGACTTCAGT TCCCAGATCT TCTCGTTGGG CCACTGTCCC 
AGTCAGGACT GGCTGGCCGT GGGGATGGAA AGCAGCCACG TGGAGGTCCT GCATGTGCGC 
AAGCCTGAGA AGTACCAGCT CCGCCTGCAC GAGAGCTGCG TGCTGTCGCT CAAGTTCGCT 
TCCTGCGGAC GCTGGTTTGT GAGCACAGGC AAGGATAACC TGCTCAATGC CTGGAGGACA 
CCCTATGGGG CCAGCATTTT CCAGTCCAAA GAGTCTTCTT CCGTGTTGAG CTGCGACATC 
TCCAGGAACA ACAAGTACAT TGTGACAGGC TCAGGGGACA AGAAGGCCAC TGTGTATGAG 
GTGGTGTACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tle2 ( XM_006240893.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006240955.1 Rattus norvegicus transducin-like enhancer of split 2 (Tle2), transcript variant X3, mRNA.

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CloneID	ORa29837
Clone ID Related Accession (Same CDS sequence)	XM_006240893.2
Accession Version	XM_006240893.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2229bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	transducin-like enhancer protein 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006240893.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACCCCC AGGGAAGGCA CCCGACCCCG CTGCAGTCTG GCCAACCTTT CAAGTTCTCA 
GTCCTGGAAA TCTGTGACCG GATCAAAGAG GAATTTCAGT TTCTCCAAGC TCAATACCAC 
AGCCTTAAGC TAGAGTGTGA GAAGCTGGCC AGCGAGAAGA CAGAAATGCA AAGGCATTAT 
GTGATGTACT ATGAGATGTC CTACGGACTC AACATTGAGA TGCACAAACA GGCTGAGATC 
GTGAAGCGCC TCAGTGCAAT CTGTGCCCAG ATGGTCCCAT TCCTCACTCA GGAGCATCAG 
CAGCAGGTGC TCCAGGCTGT GGACCGAGCC AAGCAGGTGA CCGTGGGGGA ACTGAACAAC 
CTCTTGGGGC AGAATCAGCT CCAGCCGCTG TCCCATGCAC CCCCTGTGCC TCTCACCCCA 
CGCCCAGCTG GCCTGGTGGG TGCCGGGGCC ACTGGGCTGC TGGCCCTCTC TGGGGCGCTG 
GCTGCGCAGG CCCAGCTGGT GGCTGCTGTT AAGGAAGACC GTGTGGGTGT GGACACCGAG 
GGGTCCAGAG TGGACAGAGC TGCCAGCAGG AGTTCATCCC CCTCACCCCC TGAGAGTCTG 
GCAGAAGAGG AGCATCCTAG CAGCCGAGAC AGTAACGGGA AGCAGCAGAG AGCCGAAGAC 
AAGAATATGT CAGGGCCTTA TGACAGTGAG GAAGACAAGA GTGACTATAA CCTGGTAGTG 
GATGAGGACC AACCGTCAGA GCCCCCCAGC CCTGCGACCA CCCCCTGTGG GAAGGCGCCC 
CTCTGCATCC CTGCCCGCAG GGACCTCACG GACAGTCCAG CCTCCCTGGC TTCCAGCTTG 
GGCTCACCAC TCCCCAGAAG CAAAGACCTA GCCCTGAACG ATCTCTCCAC AGGTACTCCC 
GCCTCTGGGT CATGTGGTCC CTCTCCACCC CAGGACTCGT CCACCCCAGG GCCCAGCTCA 
GCCAGTCACC TCTGCCAGCT GGCTACTCAG CCGGCACCGC CCACAGACAG CATTGCCCTG 
AGGAGTCCCC TGACCCTGTC CAGCCCTTTC ACCTCGTCCT TCAGCCTGGG CTCCCACAGC 
ACCCTCAATG GGGACCTCTC CATGCCTGGC TCCTATGTCA GCCTCCACCT GTCCCCCCAG 
GTCAGCAGCT CTGTGGTGTA CGGATGCTCA CCTCTGATGG CATTTGAGTC GCACCCCCAT 
CTCCGAGGCT CGTCCATCTC CTTGCCTAGC ATCCCGGGGG CTAAGCCGGC TTACTCCTTC 
CACGTGTCTG CGGATGGACA GATGCAACCG GTGCCCTTCC CATCGGATGC ACTGGTAGGC 
ACTGGCATCC CTCGCCACGC GAGGCAGCTG CACACACTGG CCCATGGTGA GGTGGTGTGT 
GCCGTCACCA TCAGCAGCTC CACGCAGCAC GTGTACACGG GCGGCAAAGG CTGCGTGAAG 
GTTTGGGACG TGGGCCAGCC GGGCAGCAAG ACACCTGTGG CACAGCTGGA TTGCCTGAAC 
CGGGACAACT ACATTCGCTC CTGCAAGCTG CTGCCTGACG GGCAGAGCCT GATCGTGGGT 
GGCGAGGCCA GTACCCTGTC CATTTGGGAC CTGGCAGCAC CCACACCACG CATCAAAGCA 
GAGCTGACCT CGTCCGCCCC CGCCTGTTAC GCGCTGGCCA TCAGCCCGGA TGCCAAGGTC 
TGCTTCTCCT GCTGCAGCGA CGGTAACATC GTGGTCTGGG ACCTGCAGAA CCAGGCCATG 
GTCAGACAGT TCCAGGGCCA CACGGACGGG GCCAGCTGCA TTGACATATC AGACTACGGG 
ACCCGGCTGT GGACTGGGGG CCTGGACAAC ACCGTGCGCT GCTGGGATCT GCGCGAGGGC 
CGCCAGCTGC AGCAACATGA CTTCAGTTCC CAGATCTTCT CGTTGGGCCA CTGTCCCAGT 
CAGGACTGGC TGGCCGTGGG GATGGAAAGC AGCCACGTGG AGGTCCTGCA TGTGCGCAAG 
CCTGAGAAGT ACCAGCTCCG CCTGCACGAG AGCTGCGTGC TGTCGCTCAA GTTCGCTTCC 
TGCGGACGCT GGTTTGTGAG CACAGGCAAG GATAACCTGC TCAATGCCTG GAGGACACCC 
TATGGGGCCA GCATTTTCCA GTCCAAAGAG TCTTCTTCCG TGTTGAGCTG CGACATCTCC 
AGGAACAACA AGTACATTGT GACAGGCTCA GGGGACAAGA AGGCCACTGT GTATGAGGTG 
GTGTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006240955.1
CDS	350..2578
Translation	MYPQGRHPTPLQSGQPFKFSVLEICDRIKEEFQFLQAQYHSLKLECEKLASEKTEMQRHYVMYYEMSYGLNIEMHKQAEIVKRLSAICAQMVPFLTQEHQQQVLQAVDRAKQVTVGELNNLLGQNQLQPLSHAPPVPLTPRPAGLVGAGATGLLALSGALAAQAQLVAAVKEDRVGVDTEGSRVDRAASRSSSPSPPESLAEEEHPSSRDSNGKQQRAEDKNMSGPYDSEEDKSDYNLVVDEDQPSEPPSPATTPCGKAPLCIPARRDLTDSPASLASSLGSPLPRSKDLALNDLSTGTPASGSCGPSPPQDSSTPGPSSASHLCQLATQPAPPTDSIALRSPLTLSSPFTSSFSLGSHSTLNGDLSMPGSYVSLHLSPQVSSSVVYGCSPLMAFESHPHLRGSSISLPSIPGAKPAYSFHVSADGQMQPVPFPSDALVGTGIPRHARQLHTLAHGEVVCAVTISSSTQHVYTGGKGCVKVWDVGQPGSKTPVAQLDCLNRDNYIRSCKLLPDGQSLIVGGEASTLSIWDLAAPTPRIKAELTSSAPACYALAISPDAKVCFSCCSDGNIVVWDLQNQAMVRQFQGHTDGASCIDISDYGTRLWTGGLDNTVRCWDLREGRQLQQHDFSSQIFSLGHCPSQDWLAVGMESSHVEVLHVRKPEKYQLRLHESCVLSLKFASCGRWFVSTGKDNLLNAWRTPYGASIFQSKESSSVLSCDISRNNKYIVTGSGDKKATVYEVVY

Target ORF information:

RefSeq Version	XM_006240893.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus transducin-like enhancer of split 2 (Tle2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006240893.2

ORF Insert Sequence:

ATGTACCCCC AGGGAAGGCA CCCGACCCCG CTGCAGTCTG GCCAACCTTT CAAGTTCTCA 
GTCCTGGAAA TCTGTGACCG GATCAAAGAG GAATTTCAGT TTCTCCAAGC TCAATACCAC 
AGCCTTAAGC TAGAGTGTGA GAAGCTGGCC AGCGAGAAGA CAGAAATGCA AAGGCATTAT 
GTGATGTACT ATGAGATGTC CTACGGACTC AACATTGAGA TGCACAAACA GGCTGAGATC 
GTGAAGCGCC TCAGTGCAAT CTGTGCCCAG ATGGTCCCAT TCCTCACTCA GGAGCATCAG 
CAGCAGGTGC TCCAGGCTGT GGACCGAGCC AAGCAGGTGA CCGTGGGGGA ACTGAACAAC 
CTCTTGGGGC AGAATCAGCT CCAGCCGCTG TCCCATGCAC CCCCTGTGCC TCTCACCCCA 
CGCCCAGCTG GCCTGGTGGG TGCCGGGGCC ACTGGGCTGC TGGCCCTCTC TGGGGCGCTG 
GCTGCGCAGG CCCAGCTGGT GGCTGCTGTT AAGGAAGACC GTGTGGGTGT GGACACCGAG 
GGGTCCAGAG TGGACAGAGC TGCCAGCAGG AGTTCATCCC CCTCACCCCC TGAGAGTCTG 
GCAGAAGAGG AGCATCCTAG CAGCCGAGAC AGTAACGGGA AGCAGCAGAG AGCCGAAGAC 
AAGAATATGT CAGGGCCTTA TGACAGTGAG GAAGACAAGA GTGACTATAA CCTGGTAGTG 
GATGAGGACC AACCGTCAGA GCCCCCCAGC CCTGCGACCA CCCCCTGTGG GAAGGCGCCC 
CTCTGCATCC CTGCCCGCAG GGACCTCACG GACAGTCCAG CCTCCCTGGC TTCCAGCTTG 
GGCTCACCAC TCCCCAGAAG CAAAGACCTA GCCCTGAACG ATCTCTCCAC AGGTACTCCC 
GCCTCTGGGT CATGTGGTCC CTCTCCACCC CAGGACTCGT CCACCCCAGG GCCCAGCTCA 
GCCAGTCACC TCTGCCAGCT GGCTACTCAG CCGGCACCGC CCACAGACAG CATTGCCCTG 
AGGAGTCCCC TGACCCTGTC CAGCCCTTTC ACCTCGTCCT TCAGCCTGGG CTCCCACAGC 
ACCCTCAATG GGGACCTCTC CATGCCTGGC TCCTATGTCA GCCTCCACCT GTCCCCCCAG 
GTCAGCAGCT CTGTGGTGTA CGGATGCTCA CCTCTGATGG CATTTGAGTC GCACCCCCAT 
CTCCGAGGCT CGTCCATCTC CTTGCCTAGC ATCCCGGGGG CTAAGCCGGC TTACTCCTTC 
CACGTGTCTG CGGATGGACA GATGCAACCG GTGCCCTTCC CATCGGATGC ACTGGTAGGC 
ACTGGCATCC CTCGCCACGC GAGGCAGCTG CACACACTGG CCCATGGTGA GGTGGTGTGT 
GCCGTCACCA TCAGCAGCTC CACGCAGCAC GTGTACACGG GCGGCAAAGG CTGCGTGAAG 
GTTTGGGACG TGGGCCAGCC GGGCAGCAAG ACACCTGTGG CACAGCTGGA TTGCCTGAAC 
CGGGACAACT ACATTCGCTC CTGCAAGCTG CTGCCTGACG GGCAGAGCCT GATCGTGGGT 
GGCGAGGCCA GTACCCTGTC CATTTGGGAC CTGGCAGCAC CCACACCACG CATCAAAGCA 
GAGCTGACCT CGTCCGCCCC CGCCTGTTAC GCGCTGGCCA TCAGCCCGGA TGCCAAGGTC 
TGCTTCTCCT GCTGCAGCGA CGGTAACATC GTGGTCTGGG ACCTGCAGAA CCAGGCCATG 
GTCAGACAGT TCCAGGGCCA CACGGACGGG GCCAGCTGCA TTGACATATC AGACTACGGG 
ACCCGGCTGT GGACTGGGGG CCTGGACAAC ACCGTGCGCT GCTGGGATCT GCGCGAGGGC 
CGCCAGCTGC AGCAACATGA CTTCAGTTCC CAGATCTTCT CGTTGGGCCA CTGTCCCAGT 
CAGGACTGGC TGGCCGTGGG GATGGAAAGC AGCCACGTGG AGGTCCTGCA TGTGCGCAAG 
CCTGAGAAGT ACCAGCTCCG CCTGCACGAG AGCTGCGTGC TGTCGCTCAA GTTCGCTTCC 
TGCGGACGCT GGTTTGTGAG CACAGGCAAG GATAACCTGC TCAATGCCTG GAGGACACCC 
TATGGGGCCA GCATTTTCCA GTCCAAAGAG TCTTCTTCCG TGTTGAGCTG CGACATCTCC 
AGGAACAACA AGTACATTGT GACAGGCTCA GGGGACAAGA AGGCCACTGT GTATGAGGTG 
GTGTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tle2 ( XM_006240894.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006240956.1 Rattus norvegicus transducin-like enhancer of split 2 (Tle2), transcript variant X4, mRNA.

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CloneID	ORa29838
Clone ID Related Accession (Same CDS sequence)	XM_006240894.2
Accession Version	XM_006240894.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2196bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	transducin-like enhancer protein 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006240894.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACCCCC AGGGAAGGCA CCCGACCCCG CTGCAGTCTG GCCAACCTTT CAAGTTCTCA 
GTCCTGGAAA TCTGTGACCG GATCAAAGAG GAATTTCAGT TTCTCCAAGC TCAATACCAC 
AGCCTTAAGC TAGAGTGTGA GAAGCTGGCC AGCGAGAAGA CAGAAATGCA AAGGCATTAT 
GTGATGTACT ATGAGATGTC CTACGGACTC AACATTGAGA TGCACAAACA GGCTGAGATC 
GTGAAGCGCC TCAGTGCAAT CTGTGCCCAG ATGGTCCCAT TCCTCACTCA GGAGCATCAG 
CAGCAGGTGC TCCAGGCTGT GGACCGAGCC AAGCAGGTGA CCGTGGGGGA ACTGAACAAC 
CTCTTGGGGC AGCAGAATCA GCTCCAGCCG CTGTCCCATG CACCCCCTGT GCCTCTCACC 
CCACGCCCAG CTGGCCTGGT GGGTGCCGGG GCCACTGGGC TGCTGGCCCT CTCTGGGGCG 
CTGGCTGCGC AGGCCCAGCT GGTGGCTGCT GTTAAGGAAG ACCGTGTGGG TGTGGACACC 
GAGGGGTCCA GAGTGGACAG AGCTGCCAGC AGGAGTTCAT CCCCCTCACC CCCTGAGAGT 
CTGGCAGAAG AGGAGCATCC TAGCAGCCGA GACAGTAACG GGAAGCAGCA GAGAGCCGAA 
GACAAGAATA TGTCAGGGCC TTATGACAGT GAGGAAGACA AGAGTGACTA TAACCTGGTA 
GTGGATGAGG ACCAACCGTC AGAGCCCCCC AGCCCTGCGA CCACCCCCTG TGGGAAGGCG 
CCCCTCTGCA TCCCTGCCCG CAGGGACCTC ACGGACAGTC CAGCCTCCCT GGCTTCCAGC 
TTGGGCTCAC CACTCCCCAG AAGCAAAGAC CTAGCCCTGA ACGATCTCTC CACAGGTACT 
CCCGCCTCTG GGTCATGTGG TCCCTCTCCA CCCCAGGACT CGTCCACCCC AGGGCCCAGC 
TCAGCCAGTC ACCTCTGCCA GCTGGCTACT CAGCCGGCAC CGCCCACAGA CAGCATTGCC 
CTGAGGAGTC CCCTGACCCT GTCCAGCCCT TTCACCTCGT CCTTCAGCCT GGGCTCCCAC 
AGCACCCTCA ATGGGGACCT CTCCATGCCT GGCTCCTATG TCAGCCTCCA CCTGTCCCCC 
CAGATGGCAT TTGAGTCGCA CCCCCATCTC CGAGGCTCGT CCATCTCCTT GCCTAGCATC 
CCGGGGGCTA AGCCGGCTTA CTCCTTCCAC GTGTCTGCGG ATGGACAGAT GCAACCGGTG 
CCCTTCCCAT CGGATGCACT GGTAGGCACT GGCATCCCTC GCCACGCGAG GCAGCTGCAC 
ACACTGGCCC ATGGTGAGGT GGTGTGTGCC GTCACCATCA GCAGCTCCAC GCAGCACGTG 
TACACGGGCG GCAAAGGCTG CGTGAAGGTT TGGGACGTGG GCCAGCCGGG CAGCAAGACA 
CCTGTGGCAC AGCTGGATTG CCTGAACCGG GACAACTACA TTCGCTCCTG CAAGCTGCTG 
CCTGACGGGC AGAGCCTGAT CGTGGGTGGC GAGGCCAGTA CCCTGTCCAT TTGGGACCTG 
GCAGCACCCA CACCACGCAT CAAAGCAGAG CTGACCTCGT CCGCCCCCGC CTGTTACGCG 
CTGGCCATCA GCCCGGATGC CAAGGTCTGC TTCTCCTGCT GCAGCGACGG TAACATCGTG 
GTCTGGGACC TGCAGAACCA GGCCATGGTC AGACAGTTCC AGGGCCACAC GGACGGGGCC 
AGCTGCATTG ACATATCAGA CTACGGGACC CGGCTGTGGA CTGGGGGCCT GGACAACACC 
GTGCGCTGCT GGGATCTGCG CGAGGGCCGC CAGCTGCAGC AACATGACTT CAGTTCCCAG 
ATCTTCTCGT TGGGCCACTG TCCCAGTCAG GACTGGCTGG CCGTGGGGAT GGAAAGCAGC 
CACGTGGAGG TCCTGCATGT GCGCAAGCCT GAGAAGTACC AGCTCCGCCT GCACGAGAGC 
TGCGTGCTGT CGCTCAAGTT CGCTTCCTGC GGACGCTGGT TTGTGAGCAC AGGCAAGGAT 
AACCTGCTCA ATGCCTGGAG GACACCCTAT GGGGCCAGCA TTTTCCAGTC CAAAGAGTCT 
TCTTCCGTGT TGAGCTGCGA CATCTCCAGG AACAACAAGT ACATTGTGAC AGGCTCAGGG 
GACAAGAAGG CCACTGTGTA TGAGGTGGTG TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006240956.1
CDS	350..2545
Translation	MYPQGRHPTPLQSGQPFKFSVLEICDRIKEEFQFLQAQYHSLKLECEKLASEKTEMQRHYVMYYEMSYGLNIEMHKQAEIVKRLSAICAQMVPFLTQEHQQQVLQAVDRAKQVTVGELNNLLGQQNQLQPLSHAPPVPLTPRPAGLVGAGATGLLALSGALAAQAQLVAAVKEDRVGVDTEGSRVDRAASRSSSPSPPESLAEEEHPSSRDSNGKQQRAEDKNMSGPYDSEEDKSDYNLVVDEDQPSEPPSPATTPCGKAPLCIPARRDLTDSPASLASSLGSPLPRSKDLALNDLSTGTPASGSCGPSPPQDSSTPGPSSASHLCQLATQPAPPTDSIALRSPLTLSSPFTSSFSLGSHSTLNGDLSMPGSYVSLHLSPQMAFESHPHLRGSSISLPSIPGAKPAYSFHVSADGQMQPVPFPSDALVGTGIPRHARQLHTLAHGEVVCAVTISSSTQHVYTGGKGCVKVWDVGQPGSKTPVAQLDCLNRDNYIRSCKLLPDGQSLIVGGEASTLSIWDLAAPTPRIKAELTSSAPACYALAISPDAKVCFSCCSDGNIVVWDLQNQAMVRQFQGHTDGASCIDISDYGTRLWTGGLDNTVRCWDLREGRQLQQHDFSSQIFSLGHCPSQDWLAVGMESSHVEVLHVRKPEKYQLRLHESCVLSLKFASCGRWFVSTGKDNLLNAWRTPYGASIFQSKESSSVLSCDISRNNKYIVTGSGDKKATVYEVVY

Target ORF information:

RefSeq Version	XM_006240894.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus transducin-like enhancer of split 2 (Tle2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006240894.2

ORF Insert Sequence:

ATGTACCCCC AGGGAAGGCA CCCGACCCCG CTGCAGTCTG GCCAACCTTT CAAGTTCTCA 
GTCCTGGAAA TCTGTGACCG GATCAAAGAG GAATTTCAGT TTCTCCAAGC TCAATACCAC 
AGCCTTAAGC TAGAGTGTGA GAAGCTGGCC AGCGAGAAGA CAGAAATGCA AAGGCATTAT 
GTGATGTACT ATGAGATGTC CTACGGACTC AACATTGAGA TGCACAAACA GGCTGAGATC 
GTGAAGCGCC TCAGTGCAAT CTGTGCCCAG ATGGTCCCAT TCCTCACTCA GGAGCATCAG 
CAGCAGGTGC TCCAGGCTGT GGACCGAGCC AAGCAGGTGA CCGTGGGGGA ACTGAACAAC 
CTCTTGGGGC AGCAGAATCA GCTCCAGCCG CTGTCCCATG CACCCCCTGT GCCTCTCACC 
CCACGCCCAG CTGGCCTGGT GGGTGCCGGG GCCACTGGGC TGCTGGCCCT CTCTGGGGCG 
CTGGCTGCGC AGGCCCAGCT GGTGGCTGCT GTTAAGGAAG ACCGTGTGGG TGTGGACACC 
GAGGGGTCCA GAGTGGACAG AGCTGCCAGC AGGAGTTCAT CCCCCTCACC CCCTGAGAGT 
CTGGCAGAAG AGGAGCATCC TAGCAGCCGA GACAGTAACG GGAAGCAGCA GAGAGCCGAA 
GACAAGAATA TGTCAGGGCC TTATGACAGT GAGGAAGACA AGAGTGACTA TAACCTGGTA 
GTGGATGAGG ACCAACCGTC AGAGCCCCCC AGCCCTGCGA CCACCCCCTG TGGGAAGGCG 
CCCCTCTGCA TCCCTGCCCG CAGGGACCTC ACGGACAGTC CAGCCTCCCT GGCTTCCAGC 
TTGGGCTCAC CACTCCCCAG AAGCAAAGAC CTAGCCCTGA ACGATCTCTC CACAGGTACT 
CCCGCCTCTG GGTCATGTGG TCCCTCTCCA CCCCAGGACT CGTCCACCCC AGGGCCCAGC 
TCAGCCAGTC ACCTCTGCCA GCTGGCTACT CAGCCGGCAC CGCCCACAGA CAGCATTGCC 
CTGAGGAGTC CCCTGACCCT GTCCAGCCCT TTCACCTCGT CCTTCAGCCT GGGCTCCCAC 
AGCACCCTCA ATGGGGACCT CTCCATGCCT GGCTCCTATG TCAGCCTCCA CCTGTCCCCC 
CAGATGGCAT TTGAGTCGCA CCCCCATCTC CGAGGCTCGT CCATCTCCTT GCCTAGCATC 
CCGGGGGCTA AGCCGGCTTA CTCCTTCCAC GTGTCTGCGG ATGGACAGAT GCAACCGGTG 
CCCTTCCCAT CGGATGCACT GGTAGGCACT GGCATCCCTC GCCACGCGAG GCAGCTGCAC 
ACACTGGCCC ATGGTGAGGT GGTGTGTGCC GTCACCATCA GCAGCTCCAC GCAGCACGTG 
TACACGGGCG GCAAAGGCTG CGTGAAGGTT TGGGACGTGG GCCAGCCGGG CAGCAAGACA 
CCTGTGGCAC AGCTGGATTG CCTGAACCGG GACAACTACA TTCGCTCCTG CAAGCTGCTG 
CCTGACGGGC AGAGCCTGAT CGTGGGTGGC GAGGCCAGTA CCCTGTCCAT TTGGGACCTG 
GCAGCACCCA CACCACGCAT CAAAGCAGAG CTGACCTCGT CCGCCCCCGC CTGTTACGCG 
CTGGCCATCA GCCCGGATGC CAAGGTCTGC TTCTCCTGCT GCAGCGACGG TAACATCGTG 
GTCTGGGACC TGCAGAACCA GGCCATGGTC AGACAGTTCC AGGGCCACAC GGACGGGGCC 
AGCTGCATTG ACATATCAGA CTACGGGACC CGGCTGTGGA CTGGGGGCCT GGACAACACC 
GTGCGCTGCT GGGATCTGCG CGAGGGCCGC CAGCTGCAGC AACATGACTT CAGTTCCCAG 
ATCTTCTCGT TGGGCCACTG TCCCAGTCAG GACTGGCTGG CCGTGGGGAT GGAAAGCAGC 
CACGTGGAGG TCCTGCATGT GCGCAAGCCT GAGAAGTACC AGCTCCGCCT GCACGAGAGC 
TGCGTGCTGT CGCTCAAGTT CGCTTCCTGC GGACGCTGGT TTGTGAGCAC AGGCAAGGAT 
AACCTGCTCA ATGCCTGGAG GACACCCTAT GGGGCCAGCA TTTTCCAGTC CAAAGAGTCT 
TCTTCCGTGT TGAGCTGCGA CATCTCCAGG AACAACAAGT ACATTGTGAC AGGCTCAGGG 
GACAAGAAGG CCACTGTGTA TGAGGTGGTG TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tle2 ( XM_017594719.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017450208.1 Rattus norvegicus transducin-like enhancer of split 2 (Tle2), transcript variant X5, mRNA.

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CloneID	ORa61927
Clone ID Related Accession (Same CDS sequence)	XM_017594719.1
Accession Version	XM_017594719.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2055bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	transducin-like enhancer protein 2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACCCCC AGGGAAGGCA CCCGACCCCG CTGCAGTCTG GCCAACCTTT CAAGTTCTCA 
GTCCTGGAAA TCTGTGACCG GATCAAAGAG GAATTTCAGT TTCTCCAAGC TCAATACCAC 
AGCCTTAAGC TAGAGTGTGA GAAGCTGGCC AGCGAGAAGA CAGAAATGCA AAGGCATTAT 
GTGATGTACT ATGAGATGTC CTACGGACTC AACATTGAGA TGCACAAACA GGCTGAGATC 
GTGAAGCGCC TCAGTGCAAT CTGTGCCCAG ATGGTCCCAT TCCTCACTCA GGAGCATCAG 
CAGCAGGTGC TCCAGGCTGT GGACCGAGCC AAGCAGGTGA CCGTGGGGGA ACTGAACAAC 
CTCTTGGGGC AGCAGAATCA GCTCCAGCCG CTGTCCCATG CACCCCCTGT GCCTCTCACC 
CCACGCCCAG CTGGCCTGGT GGGTGCCGGG GCCACTGGGC TGCTGGCCCT CTCTGGGGCG 
CTGGCTGCGC AGGCCCAGCT GGTGGCTGCT GTTAAGGAAG ACCGTGTGGG TGTGGACACC 
GAGGGGTCCA GAGTGGACAG AGCTGCCAGC AGGAGTTCAT CCCCCTCACC CCCTGAGAGT 
CTGGCAGAAG AGGAGCATCC TAGCAGCCGA GACAGTAACG GGAAGCAGCA GAGAGCCGAA 
GACAAGAATA TGTCAGGGCC TTATGACAGT GAGGAAGACA AGAGTGACTA TAACCTGGTA 
GTGGATGAGG ACCAACCGTC AGAGCCCCCC AGCCCTGCGA CCACCCCCTG TGGGAAGGCG 
CCCCTCTGCA TCCCTGCCCG CAGGGACCTC ACGGACAGTC CAGCCTCCCT GGCTTCCAGC 
TTGGGCTCAC CACTCCCCAG AAGCAAAGAC CTAGCCCTGG TACTCCCGCC TCTGGGTCAT 
GTGGTCCCTC TCCACCCCAG GACTCGTCCA CCCCAGGGCC CAGCTCAGCC AGTCACCTCT 
GCCAGCTGGC TACTCAGCCG GCACCGCCCA CAGACAGCAT TGATGGCATT TGAGTCGCAC 
CCCCATCTCC GAGGCTCGTC CATCTCCTTG CCTAGCATCC CGGGGGCTAA GCCGGCTTAC 
TCCTTCCACG TGTCTGCGGA TGGACAGATG CAACCGGTGC CCTTCCCATC GGATGCACTG 
GTAGGCACTG GCATCCCTCG CCACGCGAGG CAGCTGCACA CACTGGCCCA TGGTGAGGTG 
GTGTGTGCCG TCACCATCAG CAGCTCCACG CAGCACGTGT ACACGGGCGG CAAAGGCTGC 
GTGAAGGTTT GGGACGTGGG CCAGCCGGGC AGCAAGACAC CTGTGGCACA GCTGGATTGC 
CTGAACCGGG ACAACTACAT TCGCTCCTGC AAGCTGCTGC CTGACGGGCA GAGCCTGATC 
GTGGGTGGCG AGGCCAGTAC CCTGTCCATT TGGGACCTGG CAGCACCCAC ACCACGCATC 
AAAGCAGAGC TGACCTCGTC CGCCCCCGCC TGTTACGCGC TGGCCATCAG CCCGGATGCC 
AAGGTCTGCT TCTCCTGCTG CAGCGACGGT AACATCGTGG TCTGGGACCT GCAGAACCAG 
GCCATGGTCA GACAGTTCCA GGGCCACACG GACGGGGCCA GCTGCATTGA CATATCAGAC 
TACGGGACCC GGCTGTGGAC TGGGGGCCTG GACAACACCG TGCGCTGCTG GGATCTGCGC 
GAGGGCCGCC AGCTGCAGCA ACATGACTTC AGTTCCCAGA TCTTCTCGTT GGGCCACTGT 
CCCAGTCAGG ACTGGCTGGC CGTGGGGATG GAAAGCAGCC ACGTGGAGGT CCTGCATGTG 
CGCAAGCCTG AGAAGTACCA GCTCCGCCTG CACGAGAGCT GCGTGCTGTC GCTCAAGTTC 
GCTTCCTGCG GACGCTGGTT TGTGAGCACA GGCAAGGATA ACCTGCTCAA TGCCTGGAGG 
ACACCCTATG GGGCCAGCAT TTTCCAGTCC AAAGAGTCTT CTTCCGTGTT GAGCTGCGAC 
ATCTCCAGGA ACAACAAGTA CATTGTGACA GGCTCAGGGG ACAAGAAGGC CACTGTGTAT 
GAGGTGGTGT ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017450208.1
CDS	350..2404
Translation	MYPQGRHPTPLQSGQPFKFSVLEICDRIKEEFQFLQAQYHSLKLECEKLASEKTEMQRHYVMYYEMSYGLNIEMHKQAEIVKRLSAICAQMVPFLTQEHQQQVLQAVDRAKQVTVGELNNLLGQQNQLQPLSHAPPVPLTPRPAGLVGAGATGLLALSGALAAQAQLVAAVKEDRVGVDTEGSRVDRAASRSSSPSPPESLAEEEHPSSRDSNGKQQRAEDKNMSGPYDSEEDKSDYNLVVDEDQPSEPPSPATTPCGKAPLCIPARRDLTDSPASLASSLGSPLPRSKDLALVLPPLGHVVPLHPRTRPPQGPAQPVTSASWLLSRHRPQTALMAFESHPHLRGSSISLPSIPGAKPAYSFHVSADGQMQPVPFPSDALVGTGIPRHARQLHTLAHGEVVCAVTISSSTQHVYTGGKGCVKVWDVGQPGSKTPVAQLDCLNRDNYIRSCKLLPDGQSLIVGGEASTLSIWDLAAPTPRIKAELTSSAPACYALAISPDAKVCFSCCSDGNIVVWDLQNQAMVRQFQGHTDGASCIDISDYGTRLWTGGLDNTVRCWDLREGRQLQQHDFSSQIFSLGHCPSQDWLAVGMESSHVEVLHVRKPEKYQLRLHESCVLSLKFASCGRWFVSTGKDNLLNAWRTPYGASIFQSKESSSVLSCDISRNNKYIVTGSGDKKATVYEVVY

Target ORF information:

RefSeq Version	XM_017594719.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus transducin-like enhancer of split 2 (Tle2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017594719.1

ORF Insert Sequence:

ATGTACCCCC AGGGAAGGCA CCCGACCCCG CTGCAGTCTG GCCAACCTTT CAAGTTCTCA 
GTCCTGGAAA TCTGTGACCG GATCAAAGAG GAATTTCAGT TTCTCCAAGC TCAATACCAC 
AGCCTTAAGC TAGAGTGTGA GAAGCTGGCC AGCGAGAAGA CAGAAATGCA AAGGCATTAT 
GTGATGTACT ATGAGATGTC CTACGGACTC AACATTGAGA TGCACAAACA GGCTGAGATC 
GTGAAGCGCC TCAGTGCAAT CTGTGCCCAG ATGGTCCCAT TCCTCACTCA GGAGCATCAG 
CAGCAGGTGC TCCAGGCTGT GGACCGAGCC AAGCAGGTGA CCGTGGGGGA ACTGAACAAC 
CTCTTGGGGC AGCAGAATCA GCTCCAGCCG CTGTCCCATG CACCCCCTGT GCCTCTCACC 
CCACGCCCAG CTGGCCTGGT GGGTGCCGGG GCCACTGGGC TGCTGGCCCT CTCTGGGGCG 
CTGGCTGCGC AGGCCCAGCT GGTGGCTGCT GTTAAGGAAG ACCGTGTGGG TGTGGACACC 
GAGGGGTCCA GAGTGGACAG AGCTGCCAGC AGGAGTTCAT CCCCCTCACC CCCTGAGAGT 
CTGGCAGAAG AGGAGCATCC TAGCAGCCGA GACAGTAACG GGAAGCAGCA GAGAGCCGAA 
GACAAGAATA TGTCAGGGCC TTATGACAGT GAGGAAGACA AGAGTGACTA TAACCTGGTA 
GTGGATGAGG ACCAACCGTC AGAGCCCCCC AGCCCTGCGA CCACCCCCTG TGGGAAGGCG 
CCCCTCTGCA TCCCTGCCCG CAGGGACCTC ACGGACAGTC CAGCCTCCCT GGCTTCCAGC 
TTGGGCTCAC CACTCCCCAG AAGCAAAGAC CTAGCCCTGG TACTCCCGCC TCTGGGTCAT 
GTGGTCCCTC TCCACCCCAG GACTCGTCCA CCCCAGGGCC CAGCTCAGCC AGTCACCTCT 
GCCAGCTGGC TACTCAGCCG GCACCGCCCA CAGACAGCAT TGATGGCATT TGAGTCGCAC 
CCCCATCTCC GAGGCTCGTC CATCTCCTTG CCTAGCATCC CGGGGGCTAA GCCGGCTTAC 
TCCTTCCACG TGTCTGCGGA TGGACAGATG CAACCGGTGC CCTTCCCATC GGATGCACTG 
GTAGGCACTG GCATCCCTCG CCACGCGAGG CAGCTGCACA CACTGGCCCA TGGTGAGGTG 
GTGTGTGCCG TCACCATCAG CAGCTCCACG CAGCACGTGT ACACGGGCGG CAAAGGCTGC 
GTGAAGGTTT GGGACGTGGG CCAGCCGGGC AGCAAGACAC CTGTGGCACA GCTGGATTGC 
CTGAACCGGG ACAACTACAT TCGCTCCTGC AAGCTGCTGC CTGACGGGCA GAGCCTGATC 
GTGGGTGGCG AGGCCAGTAC CCTGTCCATT TGGGACCTGG CAGCACCCAC ACCACGCATC 
AAAGCAGAGC TGACCTCGTC CGCCCCCGCC TGTTACGCGC TGGCCATCAG CCCGGATGCC 
AAGGTCTGCT TCTCCTGCTG CAGCGACGGT AACATCGTGG TCTGGGACCT GCAGAACCAG 
GCCATGGTCA GACAGTTCCA GGGCCACACG GACGGGGCCA GCTGCATTGA CATATCAGAC 
TACGGGACCC GGCTGTGGAC TGGGGGCCTG GACAACACCG TGCGCTGCTG GGATCTGCGC 
GAGGGCCGCC AGCTGCAGCA ACATGACTTC AGTTCCCAGA TCTTCTCGTT GGGCCACTGT 
CCCAGTCAGG ACTGGCTGGC CGTGGGGATG GAAAGCAGCC ACGTGGAGGT CCTGCATGTG 
CGCAAGCCTG AGAAGTACCA GCTCCGCCTG CACGAGAGCT GCGTGCTGTC GCTCAAGTTC 
GCTTCCTGCG GACGCTGGTT TGTGAGCACA GGCAAGGATA ACCTGCTCAA TGCCTGGAGG 
ACACCCTATG GGGCCAGCAT TTTCCAGTCC AAAGAGTCTT CTTCCGTGTT GAGCTGCGAC 
ATCTCCAGGA ACAACAAGTA CATTGTGACA GGCTCAGGGG ACAAGAAGGC CACTGTGTAT 
GAGGTGGTGT ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Comparison of tear protein levels in breast cancer patients and healthy controls using a de novo proteomic approach.
Oncology reports28(2)429-38(2012 Aug)
B?hm D,Keller K,Pieter J,Boehm N,Wolters D,Siggelkow W,Lebrecht A,Schmidt M,K?lbl H,Pfeiffer N,Grus FH

Groucho binds two conserved regions of LEF-1 for HDAC-dependent repression.
BMC cancer9159(2009 May)
Arce L,Pate KT,Waterman ML

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Tle2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

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