Gpr65 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gpr65
Entrez Gene ID	299242
Full Name	G-protein coupled receptor 65
Synonyms	Tdag8
General protein information	Preferred Names G-protein coupled receptor 65 Names psychosine receptor T cell death associated protein 8
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 6 Map Location: 6q32

mRNA and Protein(s)

mRNA	Protein	Name
XM_008764761.2	XP_008762983.1	psychosine receptor isoform X1
NM_001106751.2	NP_001100221.1	psychosine receptor

Pathways from BioSystems

Homologies

Homo sapiens (human)	GPR65	NP_003599.2
Canis lupus familiaris (dog)	GPR65	XP_005623778.1
Rattus norvegicus (Norway rat)	Gpr65	NP_001100221.1
Gallus gallus (chicken)	GPR65	XP_004941908.1
	GPR65	XP_001082670.1
Xenopus tropicalis (tropical clawed frog)	gpr65	XP_002933239.1
Bos taurus (cattle)	GPR65	NP_001094773.1
Pan troglodytes (chimpanzee)	GPR65	XP_523005.1
Mus musculus (house mouse)	Gpr65	NP_032178.1

Gene Ontology
Bibliography

Related articles in PubMed

TDAG8 activation attenuates cerebral ischaemia-reperfusion injury via Akt signalling in rats.
Ma XD, Hang LH, Shao DH, Shu WW, Hu XL, Luo H
Experimental neurology293115-123(2017 Jul)

Acid-sensing by the T cell death-associated gene 8 (TDAG8) receptor cloned from rat brain.
McGuire J, Herman JP, Ghosal S, Eaton K, Sallee FR, Sah R
Biochemical and biophysical research communications386(3)420-5(2009 Aug)

Identification of T cell death-associated gene 8 (TDAG8) as a novel acid sensing G-protein-coupled receptor.
Ishii S, Kihara Y, Shimizu T
The Journal of biological chemistry280(10)9083-7(2005 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This study showed that TDAG8 plays an important neuroprotective role through inhibition of neuronal apoptosis and alleviation of neurological deficits by activating the Akt signalling pathway in rats.
Title: TDAG8 activation attenuates cerebral ischaemia-reperfusion injury via Akt signalling in rats.

These results open up new areas of investigation into the relevance of TDAG8 in pH homeostasis and pathological states associated with acid-base dys-regulation in the brain such as ischemia and panic disorder.
Title: Acid-sensing by the T cell death-associated gene 8 (TDAG8) receptor cloned from rat brain.

The following Gpr65 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gpr65 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa29526	NM_001106751.2 Latest version!	Rattus norvegicus G-protein coupled receptor 65 (Gpr65), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa29526	XM_008764761.2 Latest version!	Rattus norvegicus G-protein coupled receptor 65 (Gpr65), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gpr65 ( NM_001106751.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001100221.1 Rattus norvegicus G-protein coupled receptor 65 (Gpr65), mRNA.

Price & Availability↑

CloneID	ORa29526
Clone ID Related Accession (Same CDS sequence)	XM_008764761.2 , NM_001106751.2
Accession Version	NM_001106751.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1014bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-27
Organism	Rattus norvegicus(Norway rat)
Product	psychosine receptor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from EF405799.1. On Feb 24, 2008 this sequence version replaced NM_001106751.1. ##Evidence-Data-START## Transcript exon combination :: EF405799.1 [ECO:0000332] ##Evidence-Data-END##

ATGACGATGA ACAGCACATG TGTTGAAGAA CAGCATGACC TGGACCACTA TTTGTTCCCA 
GTGGTCTACA TATTTGTGTT TATAGTCAGC GTCCCAGCCA ACATCGGCTC TTTATGTGTA 
TCTTTTCTGC AAGCGAAGAA GGAAAATGAA CTAGGAGTTT ACCTCTTCAG TCTGTCACTG 
TCAGATCTGC TGTATGCGTT GACGCTGCCT CTCTGGATAA ACTACACTTG GAATAAAGAC 
AACTGGACGC TCTCTCCCAC CTTGTGCAAA GGAAGCGTTT TCTTCACCTA CCTGAACTTT 
TACAGCAGCA CGGCCTTCCT CACTTGCATT GCCTTGGACC GCTATTTAGC AGTCGTCTAC 
CCTCTAAGGT TTTCTTTTCT AAGAACAAGA AGATTTGCAT TTATCACCAG CCTGTCTATC 
TGGATATTGG AATCCGTCTT TAACTCCATC CTTCTGTGGG AAGACGAAAC AAGTGTCGAA 
TACTGTGATG CAGAGAAATC TAATTTCACT CTCTGCTATG ACAAATACCC TCTGGAGAAG 
TGGCAGATAA ACCTTAACCT GTTCAGGACT TTCCTGGGCT ACGCAATACC CTTGGTCACC 
ATCATGATCT GCAACCATAA AGTCTACCGC GCCGTGAAGC ACAACCAAGC TACGGAAAAC 
AACGAAAGGA GACGGATCAT AAAGCTGCTT GCCAGCATCA CGCTGACTTT CATCTTATGC 
TTTACCCCTT TCCACGTGAT GGTGCTCATC CGCTGCATTT TAGAGCGCAA CGTGAACTTC 
AATAACAAGA CTGGAAAGCA GACATTTACG GTGTACAGAA TCACAGTGGC CCTGACGAGT 
CTAAACTGTG TCGCCGATCC AATTCTCTAC TGTTTCGTGA CGGAAACCGG GAGATCCGAT 
ATGTGGAACA TATTAAAAGT GTGTGCTAGG AAGCGCAATA GATCCCGAGG ACAAAAAAGA 
GACATACTTT CTGTGTCCAC AAGAGATACC ATAGAACTGG AGATTATAGA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001100221.1
CDS	1..1014
Translation	MTMNSTCVEEQHDLDHYLFPVVYIFVFIVSVPANIGSLCVSFLQAKKENELGVYLFSLSLSDLLYALTLPLWINYTWNKDNWTLSPTLCKGSVFFTYLNFYSSTAFLTCIALDRYLAVVYPLRFSFLRTRRFAFITSLSIWILESVFNSILLWEDETSVEYCDAEKSNFTLCYDKYPLEKWQINLNLFRTFLGYAIPLVTIMICNHKVYRAVKHNQATENNERRRIIKLLASITLTFILCFTPFHVMVLIRCILERNVNFNNKTGKQTFTVYRITVALTSLNCVADPILYCFVTETGRSDMWNILKVCARKRNRSRGQKRDILSVSTRDTIELEIIE

Target ORF information:

RefSeq Version	NM_001106751.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus G-protein coupled receptor 65 (Gpr65), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001106751.2

ORF Insert Sequence:

ATGACGATGA ACAGCACATG TGTTGAAGAA CAGCATGACC TGGACCACTA TTTGTTCCCA 
GTGGTCTACA TATTTGTGTT TATAGTCAGC GTCCCAGCCA ACATCGGCTC TTTATGTGTA 
TCTTTTCTGC AAGCGAAGAA GGAAAATGAA CTAGGAGTTT ACCTCTTCAG TCTGTCACTG 
TCAGATCTGC TGTATGCGTT GACGCTGCCT CTCTGGATAA ACTACACTTG GAATAAAGAC 
AACTGGACGC TCTCTCCCAC CTTGTGCAAA GGAAGCGTTT TCTTCACCTA CCTGAACTTT 
TACAGCAGCA CGGCCTTCCT CACTTGCATT GCCTTGGACC GCTATTTAGC AGTCGTCTAC 
CCTCTAAGGT TTTCTTTTCT AAGAACAAGA AGATTTGCAT TTATCACCAG CCTGTCTATC 
TGGATATTGG AATCCGTCTT TAACTCCATC CTTCTGTGGG AAGACGAAAC AAGTGTCGAA 
TACTGTGATG CAGAGAAATC TAATTTCACT CTCTGCTATG ACAAATACCC TCTGGAGAAG 
TGGCAGATAA ACCTTAACCT GTTCAGGACT TTCCTGGGCT ACGCAATACC CTTGGTCACC 
ATCATGATCT GCAACCATAA AGTCTACCGC GCCGTGAAGC ACAACCAAGC TACGGAAAAC 
AACGAAAGGA GACGGATCAT AAAGCTGCTT GCCAGCATCA CGCTGACTTT CATCTTATGC 
TTTACCCCTT TCCACGTGAT GGTGCTCATC CGCTGCATTT TAGAGCGCAA CGTGAACTTC 
AATAACAAGA CTGGAAAGCA GACATTTACG GTGTACAGAA TCACAGTGGC CCTGACGAGT 
CTAAACTGTG TCGCCGATCC AATTCTCTAC TGTTTCGTGA CGGAAACCGG GAGATCCGAT 
ATGTGGAACA TATTAAAAGT GTGTGCTAGG AAGCGCAATA GATCCCGAGG ACAAAAAAGA 
GACATACTTT CTGTGTCCAC AAGAGATACC ATAGAACTGG AGATTATAGA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gpr65 ( XM_008764761.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008762983.1 Rattus norvegicus G-protein coupled receptor 65 (Gpr65), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa29526
Clone ID Related Accession (Same CDS sequence)	XM_008764761.2 , NM_001106751.2
Accession Version	XM_008764761.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1014bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	psychosine receptor isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005105.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008764761.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGATGA ACAGCACATG TGTTGAAGAA CAGCATGACC TGGACCACTA TTTGTTCCCA 
GTGGTCTACA TATTTGTGTT TATAGTCAGC GTCCCAGCCA ACATCGGCTC TTTATGTGTA 
TCTTTTCTGC AAGCGAAGAA GGAAAATGAA CTAGGAGTTT ACCTCTTCAG TCTGTCACTG 
TCAGATCTGC TGTATGCGTT GACGCTGCCT CTCTGGATAA ACTACACTTG GAATAAAGAC 
AACTGGACGC TCTCTCCCAC CTTGTGCAAA GGAAGCGTTT TCTTCACCTA CCTGAACTTT 
TACAGCAGCA CGGCCTTCCT CACTTGCATT GCCTTGGACC GCTATTTAGC AGTCGTCTAC 
CCTCTAAGGT TTTCTTTTCT AAGAACAAGA AGATTTGCAT TTATCACCAG CCTGTCTATC 
TGGATATTGG AATCCGTCTT TAACTCCATC CTTCTGTGGG AAGACGAAAC AAGTGTCGAA 
TACTGTGATG CAGAGAAATC TAATTTCACT CTCTGCTATG ACAAATACCC TCTGGAGAAG 
TGGCAGATAA ACCTTAACCT GTTCAGGACT TTCCTGGGCT ACGCAATACC CTTGGTCACC 
ATCATGATCT GCAACCATAA AGTCTACCGC GCCGTGAAGC ACAACCAAGC TACGGAAAAC 
AACGAAAGGA GACGGATCAT AAAGCTGCTT GCCAGCATCA CGCTGACTTT CATCTTATGC 
TTTACCCCTT TCCACGTGAT GGTGCTCATC CGCTGCATTT TAGAGCGCAA CGTGAACTTC 
AATAACAAGA CTGGAAAGCA GACATTTACG GTGTACAGAA TCACAGTGGC CCTGACGAGT 
CTAAACTGTG TCGCCGATCC AATTCTCTAC TGTTTCGTGA CGGAAACCGG GAGATCCGAT 
ATGTGGAACA TATTAAAAGT GTGTGCTAGG AAGCGCAATA GATCCCGAGG ACAAAAAAGA 
GACATACTTT CTGTGTCCAC AAGAGATACC ATAGAACTGG AGATTATAGA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008762983.1
CDS	520..1533
Translation	MTMNSTCVEEQHDLDHYLFPVVYIFVFIVSVPANIGSLCVSFLQAKKENELGVYLFSLSLSDLLYALTLPLWINYTWNKDNWTLSPTLCKGSVFFTYLNFYSSTAFLTCIALDRYLAVVYPLRFSFLRTRRFAFITSLSIWILESVFNSILLWEDETSVEYCDAEKSNFTLCYDKYPLEKWQINLNLFRTFLGYAIPLVTIMICNHKVYRAVKHNQATENNERRRIIKLLASITLTFILCFTPFHVMVLIRCILERNVNFNNKTGKQTFTVYRITVALTSLNCVADPILYCFVTETGRSDMWNILKVCARKRNRSRGQKRDILSVSTRDTIELEIIE

Target ORF information:

RefSeq Version	XM_008764761.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus G-protein coupled receptor 65 (Gpr65), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008764761.2

ORF Insert Sequence:

ATGACGATGA ACAGCACATG TGTTGAAGAA CAGCATGACC TGGACCACTA TTTGTTCCCA 
GTGGTCTACA TATTTGTGTT TATAGTCAGC GTCCCAGCCA ACATCGGCTC TTTATGTGTA 
TCTTTTCTGC AAGCGAAGAA GGAAAATGAA CTAGGAGTTT ACCTCTTCAG TCTGTCACTG 
TCAGATCTGC TGTATGCGTT GACGCTGCCT CTCTGGATAA ACTACACTTG GAATAAAGAC 
AACTGGACGC TCTCTCCCAC CTTGTGCAAA GGAAGCGTTT TCTTCACCTA CCTGAACTTT 
TACAGCAGCA CGGCCTTCCT CACTTGCATT GCCTTGGACC GCTATTTAGC AGTCGTCTAC 
CCTCTAAGGT TTTCTTTTCT AAGAACAAGA AGATTTGCAT TTATCACCAG CCTGTCTATC 
TGGATATTGG AATCCGTCTT TAACTCCATC CTTCTGTGGG AAGACGAAAC AAGTGTCGAA 
TACTGTGATG CAGAGAAATC TAATTTCACT CTCTGCTATG ACAAATACCC TCTGGAGAAG 
TGGCAGATAA ACCTTAACCT GTTCAGGACT TTCCTGGGCT ACGCAATACC CTTGGTCACC 
ATCATGATCT GCAACCATAA AGTCTACCGC GCCGTGAAGC ACAACCAAGC TACGGAAAAC 
AACGAAAGGA GACGGATCAT AAAGCTGCTT GCCAGCATCA CGCTGACTTT CATCTTATGC 
TTTACCCCTT TCCACGTGAT GGTGCTCATC CGCTGCATTT TAGAGCGCAA CGTGAACTTC 
AATAACAAGA CTGGAAAGCA GACATTTACG GTGTACAGAA TCACAGTGGC CCTGACGAGT 
CTAAACTGTG TCGCCGATCC AATTCTCTAC TGTTTCGTGA CGGAAACCGG GAGATCCGAT 
ATGTGGAACA TATTAAAAGT GTGTGCTAGG AAGCGCAATA GATCCCGAGG ACAAAAAAGA 
GACATACTTT CTGTGTCCAC AAGAGATACC ATAGAACTGG AGATTATAGA ATAA