Ncbp1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ncbp1
Entrez Gene ID	298075
Full Name	nuclear cap binding protein subunit 1
General protein information	Preferred Names nuclear cap binding protein subunit 1 Names nuclear cap-binding protein subunit 1 80 kDa nuclear cap-binding protein CBP80 NCBP 80 kDa subunit nuclear cap binding protein subunit 1, 80kDa
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 5 Map Location: 5q22

mRNA and Protein(s)

mRNA	Protein	Name
XM_006238037.2	XP_006238099.1	nuclear cap-binding protein subunit 1 isoform X1
NM_001014785.1	NP_001014785.1	nuclear cap-binding protein subunit 1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Ncbp1	NP_001014785.1
Xenopus tropicalis (tropical clawed frog)	ncbp1	NP_001006788.1
	NCBP1	XP_002800094.1
Canis lupus familiaris (dog)	NCBP1	XP_532003.2
Caenorhabditis elegans (roundworm)	ncbp-1	NP_491850.2
Arabidopsis thaliana (thale cress)	ABH1	NP_565356.1
Pan troglodytes (chimpanzee)	NCBP1	XP_520138.3
Oryza sativa (rice)	Os03g0347200	NP_001050097.1
Homo sapiens (human)	NCBP1	NP_002477.1
Bos taurus (cattle)	NCBP1	XP_003586450.1
Gallus gallus (chicken)	NCBP1	NP_001026611.1
Drosophila melanogaster (fruit fly)	Cbp80	NP_524750.2
Anopheles gambiae (African malaria mosquito)	NCBP1_ANOGA	XP_321964.4
Mus musculus (house mouse)	Ncbp1	NP_001028373.2

Gene Ontology
Bibliography

Related articles in PubMed

mRNA export through an additional cap-binding complex consisting of NCBP1 and NCBP3.
Gebhardt A, Habjan M, Benda C, Meiler A, Haas DA, Hein MY, Mann A, Mann M, Habermann B, Pichlmair A
Nature communications68192(2015 Sep)

The human nuclear exosome targeting complex is loaded onto newly synthesized RNA to direct early ribonucleolysis.
Lubas M, Andersen PR, Schein A, Dziembowski A, Kudla G, Jensen TH
Cell reports10(2)178-92(2015 Jan)

Cap-binding complex (CBC).
Gonatopoulos-Pournatzis T, Cowling VH
The Biochemical journal457(2)231-42(2014 Jan)

The nuclear cap-binding complex interacts with the U4/U6?U5 tri-snRNP and promotes spliceosome assembly in mammalian cells.
Pabis M, Neufeld N, Steiner MC, Bojic T, Shav-Tal Y, Neugebauer KM
RNA (New York, N.Y.)19(8)1054-63(2013 Aug)

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Baltz AG, Munschauer M, Schwanh?usser B, Vasile A, Murakawa Y, Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E, Bonneau R, Selbach M, Dieterich C, Landthaler M
Molecular cell46(5)674-90(2012 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results describe a novel mRNP complex, containing LSm1 and CBP80, that is found in neuronal dendrites throughout the central nervous system and in some axonal processes of the spinal cord.
Title: Dendritic LSm1/CBP80-mRNPs mark the early steps of transport commitment and translational control.

The following Ncbp1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ncbp1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa05620	NM_001014785.1 Latest version!	Rattus norvegicus nuclear cap binding protein subunit 1 (Ncbp1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa28125	XM_006238037.2 Latest version!	Rattus norvegicus nuclear cap binding protein subunit 1 (Ncbp1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ncbp1 ( NM_001014785.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001014785.1 Rattus norvegicus nuclear cap binding protein subunit 1 (Ncbp1), mRNA.

Price & Availability↑

CloneID	ORa05620
Clone ID Related Accession (Same CDS sequence)	NM_001014785.1
Accession Version	NM_001014785.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2373bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Rattus norvegicus(Norway rat)
Product	nuclear cap-binding protein subunit 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC092199.1. On Apr 20, 2005 this sequence version replaced XM_216408.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC092199.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGTCGCGGA GGCGGCACAG CTACGAGAAC GATGGTGGAC AACCTCACAA AAGGAGGAAG 
ACATCAGATG CAAATGAAAC GGAAGATCAT TTGGAATCTT TAATATGCAA AGTAGGGGAA 
AAGAGCGCTT GCTCTTTAGA GAGCAATCTC GAAGGCCTGG CTGGTGTTTT AGAAGCTGAT 
CTTCCTAACT ACAAGAGCAA GATCTTAAGG CTCCTTTGTA CAGTTGCACG TCTGTTACCT 
GAGAAGCTAA CAATTTATAC AACATTAGTT GGACTGCTGA ATGCCAGAAA TTATAACTTT 
GGAGGAGAAT TTGTAGAAGC TATGATTCGT CAACTTAAAG AATCGCTAAA AGCAAACAAC 
TATAATGAAG CTGTATATTT GGTCCGTTTT CTGTCTGATC TTGTGAACTG TCATGTGATT 
GCTGCCCCAT CTATGGTTGC TATGTTTGAG AATTTTGTAA GTGTAACTCA GGAAGAAGAT 
GTGCCTCAAG TGCGACGGGA TTGGTATGTG TACGCGTTTC TGTCATCCTT GCCCTGGGTT 
GGGAAGGAGT TGTATGAAAA GAAAGATGCA GAAATGGACC GCATCTTCTC CAGCACCGAA 
AGCTATCTCA AAAGACGCCA GAAGACTCAT GTGCCCATGT TACAAGTATG GACTGCCGAT 
AAGCCACACC CCCAAGAAGA GTATTTAGAT TGCCTGTGGG CCCAGATTCA GAAATTGAAA 
AAGGACCGGT GGCAGGAAAG GCACATCCTA AGACCCTACC TTGCCTTTGA CAGCATCCTC 
TGTGAAGCAC TACAGCACAA CCTGCCTCCT TTCACACCAC CGCCTCACAC CGAAGACTCC 
GTGTACCCAA TGCCCAGGGT CATCTTCAGG ATGTTTGACT ACACAGACGA CCCAGAGGGT 
CCTGTTATGC CAGGGAGCCA TTCAGTAGAG AGGTTTGTCA TAGAGGAGAA TCTTCATTGC 
ATCATAAAAT CCTACTGGAA GGAAAGGAAA ACTTGTGCTG CCCAGTTGGT GAGTTATCCA 
GGCAAGAACA AGATCCCATT GAACTACCAC ATAGTGGAGG TGATCTTTGC AGAGCTGTTT 
CAGCTTCCAG CGCCTCCGCA CATTGATGTG ATGTACACGA CACTGCTGAT CGAACTGTGC 
AAACTTCAGC CGGGCTCTCT GCCCCAAGTG CTTGCACAGG CCACTGAGAT GCTGTACATG 
CGTCTGGACA CCATGAGTAC CACGTGTGTT GACAGGTTTA TTAATTGGTT TTCTCATCAT 
CTAAGTAATT TTCAATTCCG CTGGAGCTGG GAAGATTGGT CAGATTGTCT TACTCAGGAT 
CTTGAAAGTC CCAAACCAAA GTTTGTAAGA GAAGTTCTAG AGAAATGTAT GAGGTTGTCT 
TACCATCAGC ATATATTAGA TATCGTTCCT CCCACCTTCT CAGCTCTGTG TCCTGCAAAC 
CCAACCTGCA TTTACAAGTA TGGAGACGAA AGTAGCAATT CTCTTCCTGG ACATTCCGTG 
GCACTCTGTT TATCTGTTGC TTTTAAAAGT AAGGCAACCA ACGATGAAAT CTTCAGCATT 
CTGAAGGACG TACCAAATCC TAACCAGGTT GATGATGACG ATGAAGGGTT CAGATTTAAT 
CCCTTGAAAA TAGAAGTCTT TGTCCAGACT CTGCTGCACT TAGCTGCCAA GTCCTTCAGT 
CACTCCTTCA GTGCTCTTGC AAAGTTTCAT GAAGTCTTCA AAACTCTAGC AGAAAGTGAT 
AAGGGGAAGC TGCATGTGCT GAGAGTCATG TTTGAAGTCT GGAGGAATCA CCCGCAGATG 
ATTGCTGTAC TGGTTGATAA GATGATTCGT ACACAGATTG TTGACTGTGC TGCAGTAGCA 
AACTGGATCT TCTCATCAGA GCTGTCTCGT GACTTTACGA GGTTGTTTGT GTGGGAAATT 
TTGCACTCTA CAATTCGTAA GATGAATAAG CATGTTCTGA AGATCCAGAA AGAGCTGGAA 
GAAGCTAAAG AGAAACTAGC AAGGCAGCAC AAACGACGCA GTGATGATGA TGACCGGGGC 
AGCGACAGGA AGGACGGTGC CCTGGAGGAG CAAATCGAAA GGCTGCAGGA GAAAGTGGAG 
TCTGCTCAGA GCGAGCAAAA GAACCTTTTC CTCGTCATCT TCCAGCGTTT CATCATGATC 
TTGACTGAAC ACTTGGTGCG CTGTGAAACC GATGGGACCA GTATATTGAC CCCGTGGTAT 
AAGAACTGCA TCGAGAGGCT GCAGCAGATC TTCCTACAGC ATCACCAGAT AATCCAGCAG 
TACATGGTGA CCCTGGAGAA CCTGCTCTTC ACGGCAGAGT TAGATCCTCA CATCCTGGCT 
GTGTTCCAGC AGTTCTGTGC TCTGCAGGCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001014785.1
CDS	70..2442
Translation	MSRRRHSYENDGGQPHKRRKTSDANETEDHLESLICKVGEKSACSLESNLEGLAGVLEADLPNYKSKILRLLCTVARLLPEKLTIYTTLVGLLNARNYNFGGEFVEAMIRQLKESLKANNYNEAVYLVRFLSDLVNCHVIAAPSMVAMFENFVSVTQEEDVPQVRRDWYVYAFLSSLPWVGKELYEKKDAEMDRIFSSTESYLKRRQKTHVPMLQVWTADKPHPQEEYLDCLWAQIQKLKKDRWQERHILRPYLAFDSILCEALQHNLPPFTPPPHTEDSVYPMPRVIFRMFDYTDDPEGPVMPGSHSVERFVIEENLHCIIKSYWKERKTCAAQLVSYPGKNKIPLNYHIVEVIFAELFQLPAPPHIDVMYTTLLIELCKLQPGSLPQVLAQATEMLYMRLDTMSTTCVDRFINWFSHHLSNFQFRWSWEDWSDCLTQDLESPKPKFVREVLEKCMRLSYHQHILDIVPPTFSALCPANPTCIYKYGDESSNSLPGHSVALCLSVAFKSKATNDEIFSILKDVPNPNQVDDDDEGFRFNPLKIEVFVQTLLHLAAKSFSHSFSALAKFHEVFKTLAESDKGKLHVLRVMFEVWRNHPQMIAVLVDKMIRTQIVDCAAVANWIFSSELSRDFTRLFVWEILHSTIRKMNKHVLKIQKELEEAKEKLARQHKRRSDDDDRGSDRKDGALEEQIERLQEKVESAQSEQKNLFLVIFQRFIMILTEHLVRCETDGTSILTPWYKNCIERLQQIFLQHHQIIQQYMVTLENLLFTAELDPHILAVFQQFCALQA

Target ORF information:

RefSeq Version	NM_001014785.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus nuclear cap binding protein subunit 1 (Ncbp1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001014785.1

ORF Insert Sequence:

ATGTCGCGGA GGCGGCACAG CTACGAGAAC GATGGTGGAC AACCTCACAA AAGGAGGAAG 
ACATCAGATG CAAATGAAAC GGAAGATCAT TTGGAATCTT TAATATGCAA AGTAGGGGAA 
AAGAGCGCTT GCTCTTTAGA GAGCAATCTC GAAGGCCTGG CTGGTGTTTT AGAAGCTGAT 
CTTCCTAACT ACAAGAGCAA GATCTTAAGG CTCCTTTGTA CAGTTGCACG TCTGTTACCT 
GAGAAGCTAA CAATTTATAC AACATTAGTT GGACTGCTGA ATGCCAGAAA TTATAACTTT 
GGAGGAGAAT TTGTAGAAGC TATGATTCGT CAACTTAAAG AATCGCTAAA AGCAAACAAC 
TATAATGAAG CTGTATATTT GGTCCGTTTT CTGTCTGATC TTGTGAACTG TCATGTGATT 
GCTGCCCCAT CTATGGTTGC TATGTTTGAG AATTTTGTAA GTGTAACTCA GGAAGAAGAT 
GTGCCTCAAG TGCGACGGGA TTGGTATGTG TACGCGTTTC TGTCATCCTT GCCCTGGGTT 
GGGAAGGAGT TGTATGAAAA GAAAGATGCA GAAATGGACC GCATCTTCTC CAGCACCGAA 
AGCTATCTCA AAAGACGCCA GAAGACTCAT GTGCCCATGT TACAAGTATG GACTGCCGAT 
AAGCCACACC CCCAAGAAGA GTATTTAGAT TGCCTGTGGG CCCAGATTCA GAAATTGAAA 
AAGGACCGGT GGCAGGAAAG GCACATCCTA AGACCCTACC TTGCCTTTGA CAGCATCCTC 
TGTGAAGCAC TACAGCACAA CCTGCCTCCT TTCACACCAC CGCCTCACAC CGAAGACTCC 
GTGTACCCAA TGCCCAGGGT CATCTTCAGG ATGTTTGACT ACACAGACGA CCCAGAGGGT 
CCTGTTATGC CAGGGAGCCA TTCAGTAGAG AGGTTTGTCA TAGAGGAGAA TCTTCATTGC 
ATCATAAAAT CCTACTGGAA GGAAAGGAAA ACTTGTGCTG CCCAGTTGGT GAGTTATCCA 
GGCAAGAACA AGATCCCATT GAACTACCAC ATAGTGGAGG TGATCTTTGC AGAGCTGTTT 
CAGCTTCCAG CGCCTCCGCA CATTGATGTG ATGTACACGA CACTGCTGAT CGAACTGTGC 
AAACTTCAGC CGGGCTCTCT GCCCCAAGTG CTTGCACAGG CCACTGAGAT GCTGTACATG 
CGTCTGGACA CCATGAGTAC CACGTGTGTT GACAGGTTTA TTAATTGGTT TTCTCATCAT 
CTAAGTAATT TTCAATTCCG CTGGAGCTGG GAAGATTGGT CAGATTGTCT TACTCAGGAT 
CTTGAAAGTC CCAAACCAAA GTTTGTAAGA GAAGTTCTAG AGAAATGTAT GAGGTTGTCT 
TACCATCAGC ATATATTAGA TATCGTTCCT CCCACCTTCT CAGCTCTGTG TCCTGCAAAC 
CCAACCTGCA TTTACAAGTA TGGAGACGAA AGTAGCAATT CTCTTCCTGG ACATTCCGTG 
GCACTCTGTT TATCTGTTGC TTTTAAAAGT AAGGCAACCA ACGATGAAAT CTTCAGCATT 
CTGAAGGACG TACCAAATCC TAACCAGGTT GATGATGACG ATGAAGGGTT CAGATTTAAT 
CCCTTGAAAA TAGAAGTCTT TGTCCAGACT CTGCTGCACT TAGCTGCCAA GTCCTTCAGT 
CACTCCTTCA GTGCTCTTGC AAAGTTTCAT GAAGTCTTCA AAACTCTAGC AGAAAGTGAT 
AAGGGGAAGC TGCATGTGCT GAGAGTCATG TTTGAAGTCT GGAGGAATCA CCCGCAGATG 
ATTGCTGTAC TGGTTGATAA GATGATTCGT ACACAGATTG TTGACTGTGC TGCAGTAGCA 
AACTGGATCT TCTCATCAGA GCTGTCTCGT GACTTTACGA GGTTGTTTGT GTGGGAAATT 
TTGCACTCTA CAATTCGTAA GATGAATAAG CATGTTCTGA AGATCCAGAA AGAGCTGGAA 
GAAGCTAAAG AGAAACTAGC AAGGCAGCAC AAACGACGCA GTGATGATGA TGACCGGGGC 
AGCGACAGGA AGGACGGTGC CCTGGAGGAG CAAATCGAAA GGCTGCAGGA GAAAGTGGAG 
TCTGCTCAGA GCGAGCAAAA GAACCTTTTC CTCGTCATCT TCCAGCGTTT CATCATGATC 
TTGACTGAAC ACTTGGTGCG CTGTGAAACC GATGGGACCA GTATATTGAC CCCGTGGTAT 
AAGAACTGCA TCGAGAGGCT GCAGCAGATC TTCCTACAGC ATCACCAGAT AATCCAGCAG 
TACATGGTGA CCCTGGAGAA CCTGCTCTTC ACGGCAGAGT TAGATCCTCA CATCCTGGCT 
GTGTTCCAGC AGTTCTGTGC TCTGCAGGCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ncbp1 ( XM_006238037.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006238099.1 Rattus norvegicus nuclear cap binding protein subunit 1 (Ncbp1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa28125
Clone ID Related Accession (Same CDS sequence)	XM_006238037.2
Accession Version	XM_006238037.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2052bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	nuclear cap-binding protein subunit 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005104.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006238037.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTCGTC AACTTAAAGA ATCGCTAAAA GCAAACAACT ATAATGAAGC TGTATATTTG 
GTCCGTTTTC TGTCTGATCT TGTGAACTGT CATGTGATTG CTGCCCCATC TATGGTTGCT 
ATGTTTGAGA ATTTTGTAAG TGTAACTCAG GAAGAAGATG TGCCTCAAGT GCGACGGGAT 
TGGTATGTGT ACGCGTTTCT GTCATCCTTG CCCTGGGTTG GGAAGGAGTT GTATGAAAAG 
AAAGATGCAG AAATGGACCG CATCTTCTCC AGCACCGAAA GCTATCTCAA AAGACGCCAG 
AAGACTCATG TGCCCATGTT ACAAGTATGG ACTGCCGATA AGCCACACCC CCAAGAAGAG 
TATTTAGATT GCCTGTGGGC CCAGATTCAG AAATTGAAAA AGGACCGGTG GCAGGAAAGG 
CACATCCTAA GACCCTACCT TGCCTTTGAC AGCATCCTCT GTGAAGCACT ACAGCACAAC 
CTGCCTCCTT TCACACCACC GCCTCACACC GAAGACTCCG TGTACCCAAT GCCCAGGGTC 
ATCTTCAGGA TGTTTGACTA CACAGACGAC CCAGAGGGTC CTGTTATGCC AGGGAGCCAT 
TCAGTAGAGA GGTTTGTCAT AGAGGAGAAT CTTCATTGCA TCATAAAATC CTACTGGAAG 
GAAAGGAAAA CTTGTGCTGC CCAGTTGGTG AGTTATCCAG GCAAGAACAA GATCCCATTG 
AACTACCACA TAGTGGAGGT GATCTTTGCA GAGCTGTTTC AGCTTCCAGC GCCTCCGCAC 
ATTGATGTGA TGTACACGAC ACTGCTGATC GAACTGTGCA AACTTCAGCC GGGCTCTCTG 
CCCCAAGTGC TTGCACAGGC CACTGAGATG CTGTACATGC GTCTGGACAC CATGAGTACC 
ACGTGTGTTG ACAGGTTTAT TAATTGGTTT TCTCATCATC TAAGTAATTT TCAATTCCGC 
TGGAGCTGGG AAGATTGGTC AGATTGTCTT ACTCAGGATC TTGAAAGTCC CAAACCAAAG 
TTTGTAAGAG AAGTTCTAGA GAAATGTATG AGGTTGTCTT ACCATCAGCA TATATTAGAT 
ATCGTTCCTC CCACCTTCTC AGCTCTGTGT CCTGCAAACC CAACCTGCAT TTACAAGTAT 
GGAGACGAAA GTAGCAATTC TCTTCCTGGA CATTCCGTGG CACTCTGTTT ATCTGTTGCT 
TTTAAAAGTA AGGCAACCAA CGATGAAATC TTCAGCATTC TGAAGGACGT ACCAAATCCT 
AACCAGGTTG ATGATGACGA TGAAGGGTTC AGATTTAATC CCTTGAAAAT AGAAGTCTTT 
GTCCAGACTC TGCTGCACTT AGCTGCCAAG TCCTTCAGTC ACTCCTTCAG TGCTCTTGCA 
AAGTTTCATG AAGTCTTCAA AACTCTAGCA GAAAGTGATA AGGGGAAGCT GCATGTGCTG 
AGAGTCATGT TTGAAGTCTG GAGGAATCAC CCGCAGATGA TTGCTGTACT GGTTGATAAG 
ATGATTCGTA CACAGATTGT TGACTGTGCT GCAGTAGCAA ACTGGATCTT CTCATCAGAG 
CTGTCTCGTG ACTTTACGAG GTTGTTTGTG TGGGAAATTT TGCACTCTAC AATTCGTAAG 
ATGAATAAGC ATGTTCTGAA GATCCAGAAA GAGCTGGAAG AAGCTAAAGA GAAACTAGCA 
AGGCAGCACA AACGACGCAG TGATGATGAT GACCGGGGCA GCGACAGGAA GGACGGTGCC 
CTGGAGGAGC AAATCGAAAG GCTGCAGGAG AAAGTGGAGT CTGCTCAGAG CGAGCAAAAG 
AACCTTTTCC TCGTCATCTT CCAGCGTTTC ATCATGATCT TGACTGAACA CTTGGTGCGC 
TGTGAAACCG ATGGGACCAG TATATTGACC CCGTGGTATA AGAACTGCAT CGAGAGGCTG 
CAGCAGATCT TCCTACAGCA TCACCAGATA ATCCAGCAGT ACATGGTGAC CCTGGAGAAC 
CTGCTCTTCA CGGCAGAGTT AGATCCTCAC ATCCTGGCTG TGTTCCAGCA GTTCTGTGCT 
CTGCAGGCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006238099.1
CDS	221..2272
Translation	MIRQLKESLKANNYNEAVYLVRFLSDLVNCHVIAAPSMVAMFENFVSVTQEEDVPQVRRDWYVYAFLSSLPWVGKELYEKKDAEMDRIFSSTESYLKRRQKTHVPMLQVWTADKPHPQEEYLDCLWAQIQKLKKDRWQERHILRPYLAFDSILCEALQHNLPPFTPPPHTEDSVYPMPRVIFRMFDYTDDPEGPVMPGSHSVERFVIEENLHCIIKSYWKERKTCAAQLVSYPGKNKIPLNYHIVEVIFAELFQLPAPPHIDVMYTTLLIELCKLQPGSLPQVLAQATEMLYMRLDTMSTTCVDRFINWFSHHLSNFQFRWSWEDWSDCLTQDLESPKPKFVREVLEKCMRLSYHQHILDIVPPTFSALCPANPTCIYKYGDESSNSLPGHSVALCLSVAFKSKATNDEIFSILKDVPNPNQVDDDDEGFRFNPLKIEVFVQTLLHLAAKSFSHSFSALAKFHEVFKTLAESDKGKLHVLRVMFEVWRNHPQMIAVLVDKMIRTQIVDCAAVANWIFSSELSRDFTRLFVWEILHSTIRKMNKHVLKIQKELEEAKEKLARQHKRRSDDDDRGSDRKDGALEEQIERLQEKVESAQSEQKNLFLVIFQRFIMILTEHLVRCETDGTSILTPWYKNCIERLQQIFLQHHQIIQQYMVTLENLLFTAELDPHILAVFQQFCALQA

Target ORF information:

RefSeq Version	XM_006238037.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus nuclear cap binding protein subunit 1 (Ncbp1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006238037.2

ORF Insert Sequence:

ATGATTCGTC AACTTAAAGA ATCGCTAAAA GCAAACAACT ATAATGAAGC TGTATATTTG 
GTCCGTTTTC TGTCTGATCT TGTGAACTGT CATGTGATTG CTGCCCCATC TATGGTTGCT 
ATGTTTGAGA ATTTTGTAAG TGTAACTCAG GAAGAAGATG TGCCTCAAGT GCGACGGGAT 
TGGTATGTGT ACGCGTTTCT GTCATCCTTG CCCTGGGTTG GGAAGGAGTT GTATGAAAAG 
AAAGATGCAG AAATGGACCG CATCTTCTCC AGCACCGAAA GCTATCTCAA AAGACGCCAG 
AAGACTCATG TGCCCATGTT ACAAGTATGG ACTGCCGATA AGCCACACCC CCAAGAAGAG 
TATTTAGATT GCCTGTGGGC CCAGATTCAG AAATTGAAAA AGGACCGGTG GCAGGAAAGG 
CACATCCTAA GACCCTACCT TGCCTTTGAC AGCATCCTCT GTGAAGCACT ACAGCACAAC 
CTGCCTCCTT TCACACCACC GCCTCACACC GAAGACTCCG TGTACCCAAT GCCCAGGGTC 
ATCTTCAGGA TGTTTGACTA CACAGACGAC CCAGAGGGTC CTGTTATGCC AGGGAGCCAT 
TCAGTAGAGA GGTTTGTCAT AGAGGAGAAT CTTCATTGCA TCATAAAATC CTACTGGAAG 
GAAAGGAAAA CTTGTGCTGC CCAGTTGGTG AGTTATCCAG GCAAGAACAA GATCCCATTG 
AACTACCACA TAGTGGAGGT GATCTTTGCA GAGCTGTTTC AGCTTCCAGC GCCTCCGCAC 
ATTGATGTGA TGTACACGAC ACTGCTGATC GAACTGTGCA AACTTCAGCC GGGCTCTCTG 
CCCCAAGTGC TTGCACAGGC CACTGAGATG CTGTACATGC GTCTGGACAC CATGAGTACC 
ACGTGTGTTG ACAGGTTTAT TAATTGGTTT TCTCATCATC TAAGTAATTT TCAATTCCGC 
TGGAGCTGGG AAGATTGGTC AGATTGTCTT ACTCAGGATC TTGAAAGTCC CAAACCAAAG 
TTTGTAAGAG AAGTTCTAGA GAAATGTATG AGGTTGTCTT ACCATCAGCA TATATTAGAT 
ATCGTTCCTC CCACCTTCTC AGCTCTGTGT CCTGCAAACC CAACCTGCAT TTACAAGTAT 
GGAGACGAAA GTAGCAATTC TCTTCCTGGA CATTCCGTGG CACTCTGTTT ATCTGTTGCT 
TTTAAAAGTA AGGCAACCAA CGATGAAATC TTCAGCATTC TGAAGGACGT ACCAAATCCT 
AACCAGGTTG ATGATGACGA TGAAGGGTTC AGATTTAATC CCTTGAAAAT AGAAGTCTTT 
GTCCAGACTC TGCTGCACTT AGCTGCCAAG TCCTTCAGTC ACTCCTTCAG TGCTCTTGCA 
AAGTTTCATG AAGTCTTCAA AACTCTAGCA GAAAGTGATA AGGGGAAGCT GCATGTGCTG 
AGAGTCATGT TTGAAGTCTG GAGGAATCAC CCGCAGATGA TTGCTGTACT GGTTGATAAG 
ATGATTCGTA CACAGATTGT TGACTGTGCT GCAGTAGCAA ACTGGATCTT CTCATCAGAG 
CTGTCTCGTG ACTTTACGAG GTTGTTTGTG TGGGAAATTT TGCACTCTAC AATTCGTAAG 
ATGAATAAGC ATGTTCTGAA GATCCAGAAA GAGCTGGAAG AAGCTAAAGA GAAACTAGCA 
AGGCAGCACA AACGACGCAG TGATGATGAT GACCGGGGCA GCGACAGGAA GGACGGTGCC 
CTGGAGGAGC AAATCGAAAG GCTGCAGGAG AAAGTGGAGT CTGCTCAGAG CGAGCAAAAG 
AACCTTTTCC TCGTCATCTT CCAGCGTTTC ATCATGATCT TGACTGAACA CTTGGTGCGC 
TGTGAAACCG ATGGGACCAG TATATTGACC CCGTGGTATA AGAACTGCAT CGAGAGGCTG 
CAGCAGATCT TCCTACAGCA TCACCAGATA ATCCAGCAGT ACATGGTGAC CCTGGAGAAC 
CTGCTCTTCA CGGCAGAGTT AGATCCTCAC ATCCTGGCTG TGTTCCAGCA GTTCTGTGCT 
CTGCAGGCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

mRNA export through an additional cap-binding complex consisting of NCBP1 and NCBP3.
Nature communications68192(2015 Sep)
Gebhardt A,Habjan M,Benda C,Meiler A,Haas DA,Hein MY,Mann A,Mann M,Habermann B,Pichlmair A

The human nuclear exosome targeting complex is loaded onto newly synthesized RNA to direct early ribonucleolysis.
Cell reports10(2)178-92(2015 Jan)
Lubas M,Andersen PR,Schein A,Dziembowski A,Kudla G,Jensen TH

Cap-binding complex (CBC).
The Biochemical journal457(2)231-42(2014 Jan)
Gonatopoulos-Pournatzis T,Cowling VH

The nuclear cap-binding complex interacts with the U4/U6?U5 tri-snRNP and promotes spliceosome assembly in mammalian cells.
RNA (New York, N.Y.)19(8)1054-63(2013 Aug)
Pabis M,Neufeld N,Steiner MC,Bojic T,Shav-Tal Y,Neugebauer KM

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Molecular cell46(5)674-90(2012 Jun)
Baltz AG,Munschauer M,Schwanh?usser B,Vasile A,Murakawa Y,Schueler M,Youngs N,Penfold-Brown D,Drew K,Milek M,Wyler E,Bonneau R,Selbach M,Dieterich C,Landthaler M

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Ncbp1 cDNA ORF clone, Rattus norvegicus(Norway rat)

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Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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