Gem cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gem
Entrez Gene ID	297902
Full Name	GTP binding protein overexpressed in skeletal muscle
General protein information	Preferred Names GTP binding protein overexpressed in skeletal muscle Names GTP-binding protein GEM GTP binding protein (gene overexpressed in skeletal muscle)
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 5 Map Location: 5q13

mRNA and Protein(s)

mRNA	Protein	Name
NM_001106637.1	NP_001100107.1	GTP-binding protein GEM

Pathways from BioSystems

Homologies

	GEM	XP_001087974.1
Danio rerio (zebrafish)	gem	NP_001039314.1
Rattus norvegicus (Norway rat)	Gem	NP_001100107.1
Xenopus tropicalis (tropical clawed frog)	gem	NP_001090822.1
Homo sapiens (human)	GEM	NP_859053.1
Gallus gallus (chicken)	GEM	NP_998744.1
Pan troglodytes (chimpanzee)	GEM	XP_001142383.1
Canis lupus familiaris (dog)	GEM	XP_854414.2
Bos taurus (cattle)	GEM	NP_001263234.1
Mus musculus (house mouse)	Gem	NP_034406.2

Gene Ontology
Bibliography

Related articles in PubMed

Upregulation of Gem relates to retinal ganglion cells apoptosis after optic nerve crush in adult rats.
Xu F, Huang H, Wu Y, Lu L, Jiang L, Chen L, Zeng S, Li L, Li M
Journal of molecular histology45(5)565-71(2014 Oct)

Spatiotemporal patterns of Gem expression after rat spinal cord injury.
Wen H, Cao J, Yu X, Sun B, Ding T, Li M, Li D, Wu H, Long L, Xu G, Zhang F
Brain research151611-9(2013 Jun)

Increased expression of Gem after rat sciatic nerve injury.
Wang Y, Cheng X, Zhou Z, Wu H, Long L, Gu X, Xu G
Journal of molecular histology44(1)27-36(2013 Feb)

The GTPase Gem and its partner Kif9 are required for chromosome alignment, spindle length control, and mitotic progression.
Andrieu G, Quaranta M, Leprince C, Hatzoglou A
FASEB journal : official publication of the Federation of American Societies for Experimental Biology26(12)5025-34(2012 Dec)

A proteomic comparison of immature and mature mouse gonadotrophs reveals novel differentially expressed nuclear proteins that regulate gonadotropin gene transcription and RNA splicing.
Feng J, Lawson MA, Melamed P
Biology of reproduction79(3)546-61(2008 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest that GTP-binding protein Gem might play a crucial role in retinal ganglion cells (RGCs) apoptosis after nerve crush (ONC).
Title: Upregulation of Gem relates to retinal ganglion cells apoptosis after optic nerve crush in adult rats.

The results of tjis study suggested that Gem may be implicated in the proliferation of microglia and astrocytes after spinal cord injury.
Title: Spatiotemporal patterns of Gem expression after rat spinal cord injury.

Increased expression of Gem after rat sciatic nerve injury.
Title: Increased expression of Gem after rat sciatic nerve injury.

binding of 14-3-3, calmodulin and calcium channel beta-subunits to Kir/Gem is mutually exclusive
Title: 14-3-3 and calmodulin control subcellular distribution of Kir/Gem and its regulation of cell shape and calcium channel activity.

The following Gem gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gem cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa04871	NM_001106637.1 Latest version!	Rattus norvegicus GTP binding protein overexpressed in skeletal muscle (Gem), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gem ( NM_001106637.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001100107.1 Rattus norvegicus GTP binding protein overexpressed in skeletal muscle (Gem), mRNA.

Price & Availability↑

CloneID	ORa04871
Clone ID Related Accession (Same CDS sequence)	NM_001106637.1
Accession Version	NM_001106637.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 888bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-27
Organism	Rattus norvegicus(Norway rat)
Product	GTP-binding protein GEM
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473962.1. On or before Oct 4, 2007 this sequence version replaced XM_232788.4, XM_001054224.1. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGACTCTGA ATAACGTCAC CATGCGCCAA GGCACTGTGG GCATGCAGCC ACAGCAACGC 
TGGAGCATCC CTGCTGATGG CAGGCATCTG ATGGTCCAGA AGGATCCCCA CCCCTGCAAC 
CCCCACAACC ACCACTCCAC TGCTCCCGAT GACCACTGCC GGCGGAGCTG GTCCTCCGAG 
TCCACAGACT CGGTTATCTC TTCCGAGTCA GGAAACACCT ACTACCGAGT GGTGCTTATT 
GGGGAGCAAG GAGTGGGCAA GTCCACCCTG GCCAACATCT TTGCAGGTGT ACATGACAGC 
ATGGACAGCG ACTGTGAGGT CTTGGGAGAA GATACATATG AGCGTACCCT GGTCGTTGAT 
GGAGAGAGTG CAACCATTAT CCTACTGGAC ATGTGGGAAA ATAAGGGGGA GAATGAATGG 
CTCCACGACC ACTGCATGCA GGTCGGGGAC GCCTACCTGA TCGTCTACTC CATCACAGAC 
CGGGCGAGCT TTGAGAAGGC GTCTGAGCTG AGGATCCAGC TCCGCAGGGC CCGGCAGACA 
GAAGACATTC CTATAATTTT GGTTGGCAAC AAAAGCGACT TAGTGCGGTG TCGAGAAGTG 
TCTGTGTCAG AAGGGAGAGC TTGTGCCGTG GTGTTCGACT GCAAATTCAT CGAGACCTCT 
GCAGCCGTGC AGCACAACGT GAAGGAACTG TTTGAGGGCA TTGTGCGGCA GGTCCGTCTG 
CGTCGGGACA GCAAGGAAAA GAACGAGAGG AGGCTGGCCT ACCAGAAGAG GCGGGAGAGT 
ATCCCCAGGA AAGCCAGACG CTTCTGGGGC AAAATTGTAG CCAAAAACAA CAAGAACATG 
GCTTTCAAGC TCAAGTCAAA ATCCTGCCAT GACCTGTCTG TGCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001100107.1
CDS	147..1034
Translation	MTLNNVTMRQGTVGMQPQQRWSIPADGRHLMVQKDPHPCNPHNHHSTAPDDHCRRSWSSESTDSVISSESGNTYYRVVLIGEQGVGKSTLANIFAGVHDSMDSDCEVLGEDTYERTLVVDGESATIILLDMWENKGENEWLHDHCMQVGDAYLIVYSITDRASFEKASELRIQLRRARQTEDIPIILVGNKSDLVRCREVSVSEGRACAVVFDCKFIETSAAVQHNVKELFEGIVRQVRLRRDSKEKNERRLAYQKRRESIPRKARRFWGKIVAKNNKNMAFKLKSKSCHDLSVL

Target ORF information:

RefSeq Version	NM_001106637.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus GTP binding protein overexpressed in skeletal muscle (Gem), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001106637.1

ORF Insert Sequence:

ATGACTCTGA ATAACGTCAC CATGCGCCAA GGCACTGTGG GCATGCAGCC ACAGCAACGC 
TGGAGCATCC CTGCTGATGG CAGGCATCTG ATGGTCCAGA AGGATCCCCA CCCCTGCAAC 
CCCCACAACC ACCACTCCAC TGCTCCCGAT GACCACTGCC GGCGGAGCTG GTCCTCCGAG 
TCCACAGACT CGGTTATCTC TTCCGAGTCA GGAAACACCT ACTACCGAGT GGTGCTTATT 
GGGGAGCAAG GAGTGGGCAA GTCCACCCTG GCCAACATCT TTGCAGGTGT ACATGACAGC 
ATGGACAGCG ACTGTGAGGT CTTGGGAGAA GATACATATG AGCGTACCCT GGTCGTTGAT 
GGAGAGAGTG CAACCATTAT CCTACTGGAC ATGTGGGAAA ATAAGGGGGA GAATGAATGG 
CTCCACGACC ACTGCATGCA GGTCGGGGAC GCCTACCTGA TCGTCTACTC CATCACAGAC 
CGGGCGAGCT TTGAGAAGGC GTCTGAGCTG AGGATCCAGC TCCGCAGGGC CCGGCAGACA 
GAAGACATTC CTATAATTTT GGTTGGCAAC AAAAGCGACT TAGTGCGGTG TCGAGAAGTG 
TCTGTGTCAG AAGGGAGAGC TTGTGCCGTG GTGTTCGACT GCAAATTCAT CGAGACCTCT 
GCAGCCGTGC AGCACAACGT GAAGGAACTG TTTGAGGGCA TTGTGCGGCA GGTCCGTCTG 
CGTCGGGACA GCAAGGAAAA GAACGAGAGG AGGCTGGCCT ACCAGAAGAG GCGGGAGAGT 
ATCCCCAGGA AAGCCAGACG CTTCTGGGGC AAAATTGTAG CCAAAAACAA CAAGAACATG 
GCTTTCAAGC TCAAGTCAAA ATCCTGCCAT GACCTGTCTG TGCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Upregulation of Gem relates to retinal ganglion cells apoptosis after optic nerve crush in adult rats.
Journal of molecular histology45(5)565-71(2014 Oct)
Xu F,Huang H,Wu Y,Lu L,Jiang L,Chen L,Zeng S,Li L,Li M

Spatiotemporal patterns of Gem expression after rat spinal cord injury.
Brain research151611-9(2013 Jun)
Wen H,Cao J,Yu X,Sun B,Ding T,Li M,Li D,Wu H,Long L,Xu G,Zhang F

Increased expression of Gem after rat sciatic nerve injury.
Journal of molecular histology44(1)27-36(2013 Feb)
Wang Y,Cheng X,Zhou Z,Wu H,Long L,Gu X,Xu G

The GTPase Gem and its partner Kif9 are required for chromosome alignment, spindle length control, and mitotic progression.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology26(12)5025-34(2012 Dec)
Andrieu G,Quaranta M,Leprince C,Hatzoglou A

A proteomic comparison of immature and mature mouse gonadotrophs reveals novel differentially expressed nuclear proteins that regulate gonadotropin gene transcription and RNA splicing.
Biology of reproduction79(3)546-61(2008 Sep)
Feng J,Lawson MA,Melamed P