Ndufaf6 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ndufaf6
Entrez Gene ID	297821
Full Name	NADH:ubiquinone oxidoreductase complex assembly factor 6
Synonyms	RGD1309085
General protein information	Preferred Names NADH:ubiquinone oxidoreductase complex assembly factor 6 Names NADH dehydrogenase (ubiquinone) complex I, assembly factor 6
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 5 Map Location: 5q13

mRNA and Protein(s)

mRNA	Protein	Name
NM_001276441.1	NP_001263370.1	NADH dehydrogenase (ubiquinone) complex I, assembly factor 6 precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	NDUFAF6	XP_001144499.1
Drosophila melanogaster (fruit fly)	CG15738	NP_572765.1
	NDUFAF6	XP_001090361.1
Rattus norvegicus (Norway rat)	Ndufaf6	NP_001263370.1
Gallus gallus (chicken)	C2H8ORF38	XP_418344.4
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000326	XP_310791.5
Magnaporthe oryzae (rice blast fungus)	MGG_05031	XP_003712539.1
Bos taurus (cattle)	NDUFAF6	NP_001098859.1
Neurospora crassa	NCU09517	XP_958512.2
Oryza sativa (rice)	Os06g0104100	NP_001056548.1
Xenopus tropicalis (tropical clawed frog)	ndufaf6	XP_002931576.1
Caenorhabditis elegans (roundworm)	CELE_B0334.5	NP_496458.3
Arabidopsis thaliana (thale cress)	AT1G62730	NP_564800.1
Homo sapiens (human)	NDUFAF6	NP_689629.2
Canis lupus familiaris (dog)	NDUFAF6	XP_535131.2
Mus musculus (house mouse)	Ndufaf6	NP_001078962.1
Danio rerio (zebrafish)	ndufaf6	XP_698183.3

Gene Ontology
Bibliography

Related articles in PubMed

Mutations in the gene encoding C8orf38 block complex I assembly by inhibiting production of the mitochondria-encoded subunit ND1.
McKenzie M, Tucker EJ, Compton AG, Lazarou M, George C, Thorburn DR, Ryan MT
Journal of molecular biology414(3)413-26(2011 Dec)

Assembly factors of human mitochondrial complex I and their defects in disease.
Mckenzie M, Ryan MT
IUBMB life62(7)497-502(2010 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This review summarizes the current model of human complex I assembly and the roles played by different assembly factors at early, mid, and late assembly stages. For complex I assembly to proceed effectively in forming the holoenzyme, a group of proteins termed ""assembly factors"" are required. Defects in assembly factors which disrupt complex I assembly may contribute to human disease pathogenesis.
Title: Assembly factors of human mitochondrial complex I and their defects in disease.

The following Ndufaf6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ndufaf6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa12252	NM_001276441.1 Latest version!	Rattus norvegicus NADH:ubiquinone oxidoreductase complex assembly factor 6 (Ndufaf6), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ndufaf6 ( NM_001276441.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001263370.1 Rattus norvegicus NADH:ubiquinone oxidoreductase complex assembly factor 6 (Ndufaf6), mRNA.

Price & Availability↑

CloneID	ORa12252
Clone ID Related Accession (Same CDS sequence)	NM_001276441.1
Accession Version	NM_001276441.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1002bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-14
Organism	Rattus norvegicus(Norway rat)
Product	NADH dehydrogenase (ubiquinone) complex I, assembly factor 6 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07047213.1. On or before Feb 16, 2013 this sequence version replaced XM_001072347.3, XM_232684.5. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: FQ215404.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGACCT CCATGCTGGG ATCAGTCCCC GGTCCAAGGC CGTTCGGTTT GGCTGGTCTG 
TTCCGTCGCC GACCGCCTCG GGATCCGTGG GAACGTGTGC GGCGCCTGCC GAGGCTCTCG 
GCAGTCCGGC GCAGCGTGGC TGCAGCCAGC GGGCCGGGCA TCCCGGATTC CCACCTCTAT 
TGCCTGGAGC TGCTGAGGAA ACGGGATTAT GAAAGCTACT TGTGTTCCTT ACTGTTCCCT 
GCAGAGTGTC AAAGGTCTGC TTCTGCACTG CGGGCCTTCA ATGTGGAACT GGCTCAGGTT 
AAGGACTCGG TCTCTGAGAA AACAATTGGC CTGATGCGCA TGCAGTTCTG GAGAAAAGCT 
GTGGAAGACA TGTTCTGTGA CAACCCGCCA CACCAGCCAG TGGCCATGGA GCTCTGGAAG 
GCTGTCAAGA GACATAACCT AACTAAAAGG TGGCTCCTGA GAATCATCGA TGAAAGAGAG 
AAAAACCTGG ATGACAAAGC ATACCGCAGC ATCCGGGAAC TTGAAACCTA CGCTGAGAAC 
ACGCAGAGTT CCCTGCTCTA CCTGACACTG GAGGTGCTGG GCGTGAAGGA TGTTCATGCA 
GATCATGCTG CAAGCCACAT TGGAAAGGCA CAAGGCATTG TGACATGCTT GAGAGCAACT 
CCCTATCACA GTAGCCGGAG ACAAGTATTC CTGCCCATGG ATGTGTGTGT ACAGCATGGT 
GTTTCACAAG AAGACTTTCT ACGGAGAAAT CAAGATAAAA ATGTGAGAGA TGTAGTATAT 
GACATTGCCA GCCAGGCACA CTTGCACCTA AAGCATGCGA GGTCCTTCCA CAGTCGCGTT 
CCTGCCGAAG CATTTCCTGC TTTTCTTCAG ACGGTTTCTC TAGAGGACTA TTTAAAGAAA 
ATCCAGCGAG TGGACTTTGA TATATTCCAT CCGTCTTTAC AGCAGAAGAA TACGTTACTT 
CCGTTATCTT TATATATTCA ATCATGGAGA AAAAGATACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001263370.1
CDS	12..1013
Translation	MATSMLGSVPGPRPFGLAGLFRRRPPRDPWERVRRLPRLSAVRRSVAAASGPGIPDSHLYCLELLRKRDYESYLCSLLFPAECQRSASALRAFNVELAQVKDSVSEKTIGLMRMQFWRKAVEDMFCDNPPHQPVAMELWKAVKRHNLTKRWLLRIIDEREKNLDDKAYRSIRELETYAENTQSSLLYLTLEVLGVKDVHADHAASHIGKAQGIVTCLRATPYHSSRRQVFLPMDVCVQHGVSQEDFLRRNQDKNVRDVVYDIASQAHLHLKHARSFHSRVPAEAFPAFLQTVSLEDYLKKIQRVDFDIFHPSLQQKNTLLPLSLYIQSWRKRY

Target ORF information:

RefSeq Version	NM_001276441.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus NADH:ubiquinone oxidoreductase complex assembly factor 6 (Ndufaf6), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001276441.1

ORF Insert Sequence:

ATGGCGACCT CCATGCTGGG ATCAGTCCCC GGTCCAAGGC CGTTCGGTTT GGCTGGTCTG 
TTCCGTCGCC GACCGCCTCG GGATCCGTGG GAACGTGTGC GGCGCCTGCC GAGGCTCTCG 
GCAGTCCGGC GCAGCGTGGC TGCAGCCAGC GGGCCGGGCA TCCCGGATTC CCACCTCTAT 
TGCCTGGAGC TGCTGAGGAA ACGGGATTAT GAAAGCTACT TGTGTTCCTT ACTGTTCCCT 
GCAGAGTGTC AAAGGTCTGC TTCTGCACTG CGGGCCTTCA ATGTGGAACT GGCTCAGGTT 
AAGGACTCGG TCTCTGAGAA AACAATTGGC CTGATGCGCA TGCAGTTCTG GAGAAAAGCT 
GTGGAAGACA TGTTCTGTGA CAACCCGCCA CACCAGCCAG TGGCCATGGA GCTCTGGAAG 
GCTGTCAAGA GACATAACCT AACTAAAAGG TGGCTCCTGA GAATCATCGA TGAAAGAGAG 
AAAAACCTGG ATGACAAAGC ATACCGCAGC ATCCGGGAAC TTGAAACCTA CGCTGAGAAC 
ACGCAGAGTT CCCTGCTCTA CCTGACACTG GAGGTGCTGG GCGTGAAGGA TGTTCATGCA 
GATCATGCTG CAAGCCACAT TGGAAAGGCA CAAGGCATTG TGACATGCTT GAGAGCAACT 
CCCTATCACA GTAGCCGGAG ACAAGTATTC CTGCCCATGG ATGTGTGTGT ACAGCATGGT 
GTTTCACAAG AAGACTTTCT ACGGAGAAAT CAAGATAAAA ATGTGAGAGA TGTAGTATAT 
GACATTGCCA GCCAGGCACA CTTGCACCTA AAGCATGCGA GGTCCTTCCA CAGTCGCGTT 
CCTGCCGAAG CATTTCCTGC TTTTCTTCAG ACGGTTTCTC TAGAGGACTA TTTAAAGAAA 
ATCCAGCGAG TGGACTTTGA TATATTCCAT CCGTCTTTAC AGCAGAAGAA TACGTTACTT 
CCGTTATCTT TATATATTCA ATCATGGAGA AAAAGATACT AA