Gars cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gars
Entrez Gene ID	297113
Full Name	glycyl-tRNA synthetase
Synonyms	GlyRS,RGD1559871
General protein information	Preferred Names glycyl-tRNA synthetase Names glycine--tRNA ligase AP-4-A synthetase diadenosine tetraphosphate synthetase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 4 Map Location: 4q24

mRNA and Protein(s)

mRNA	Protein	Name
NM_001271139.2	NP_001258068.1	glycine--tRNA ligase

Pathways from BioSystems

Homologies

Homo sapiens (human)	GARS	NP_002038.2
Drosophila melanogaster (fruit fly)	Aats-gly	NP_001261867.1
Saccharomyces cerevisiae (baker's yeast)	GRS1	NP_009679.2
Magnaporthe oryzae (rice blast fungus)	MGG_06321	XP_003717264.1
Neurospora crassa	NCU00405	XP_957291.1
Xenopus tropicalis (tropical clawed frog)	gars	NP_001007492.1
Pan troglodytes (chimpanzee)	GARS	XP_519025.3
Bos taurus (cattle)	GARS	NP_001091035.1
Rattus norvegicus (Norway rat)	Gars	NP_001258068.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008604	XP_557743.2
Caenorhabditis elegans (roundworm)	gars-1	NP_498093.1
Gallus gallus (chicken)	GARS	NP_001026681.1
Oryza sativa (rice)	Os08g0538000	NP_001062369.1
Kluyveromyces lactis	KLLA0B08932g	XP_451926.1
Eremothecium gossypii	AGOS_ADL373W	NP_983723.1
Schizosaccharomyces pombe (fission yeast)	grs1	NP_593935.1
Canis lupus familiaris (dog)	GARS	XP_003639538.1
Mus musculus (house mouse)	Gars	NP_851009.2
Danio rerio (zebrafish)	gars	XP_692410.4
Arabidopsis thaliana (thale cress)	AT1G29880	NP_564337.1

Gene Ontology
Bibliography

Related articles in PubMed

Compound heterozygosity for loss-of-function GARS variants results in a multisystem developmental syndrome that includes severe growth retardation.
Oprescu SN, Chepa-Lotrea X, Takase R, Golas G, Markello TC, Adams DR, Toro C, Gropman AL, Hou YM, Malicdan MCV, Gahl WA, Tifft CJ, Antonellis A
Human mutation38(10)1412-1420(2017 Oct)

CMT2D neuropathy is linked to the neomorphic binding activity of glycyl-tRNA synthetase.
He W, Bai G, Zhou H, Wei N, White NM, Lauer J, Liu H, Shi Y, Dumitru CD, Lettieri K, Shubayev V, Jordanova A, Guergueltcheva V, Griffin PR, Burgess RW, Pfaff SL, Yang XL
Nature526(7575)710-4(2015 Oct)

Crystal structures and biochemical analyses suggest a unique mechanism and role for human glycyl-tRNA synthetase in Ap4A homeostasis.
Guo RT, Chong YE, Guo M, Yang XL
The Journal of biological chemistry284(42)28968-76(2009 Oct)

An ENU-induced mutation in mouse glycyl-tRNA synthetase (GARS) causes peripheral sensory and motor phenotypes creating a model of Charcot-Marie-Tooth type 2D peripheral neuropathy.
Achilli F, Bros-Facer V, Williams HP, Banks GT, AlQatari M, Chia R, Tucci V, Groves M, Nickols CD, Seburn KL, Kendall R, Cader MZ, Talbot K, van Minnen J, Burgess RW, Brandner S, Martin JE, Koltzenburg M, Greensmith L, Nolan PM, Fisher EM
Disease models & mechanisms2(7-8)359-73(2009 Jul-Aug)

Charcot-Marie-Tooth disease-associated mutant tRNA synthetases linked to altered dimer interface and neurite distribution defect.
Nangle LA, Zhang W, Xie W, Yang XL, Schimmel P
Proceedings of the National Academy of Sciences of the United States of America104(27)11239-44(2007 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Gars gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gars cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa10396	NM_001271139.2 Latest version!	Rattus norvegicus glycyl-tRNA synthetase (Gars), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gars ( NM_001271139.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001258068.1 Rattus norvegicus glycyl-tRNA synthetase (Gars), mRNA.

Price & Availability↑

CloneID	ORa10396
Clone ID Related Accession (Same CDS sequence)	NM_001271139.2
Accession Version	NM_001271139.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2187bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-16
Organism	Rattus norvegicus(Norway rat)
Product	glycine--tRNA ligase
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07060610.1 and AABR07060611.1. On Sep 18, 2014 this sequence version replaced NM_001271139.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGCCCTGTC TGCTCCCCAC GCTGCTCAGA GCCACCCGCG CCGCGCTGCT CCTACAGTCG 
CCCCGAGTGG TCGCAGCGCC CGCGTCTCAG CGGCTCCTCA GCGCTCCCGC GCAGCCCGCC 
GCCTCTCCGA GCAGCATGGA CAGCGCCGAG GAGCTGCTAG CCCCATTGCG GCTAGCTGTG 
CGCCAGCAGG GAGACTTTGT ACGGAAGTTA AAAGAAGATA AAGCCCCACA AGTGGATGTG 
GACAGAGCAG TAGCTGAGCT CAAGGCCCGG AAGAGGGTTC TGGAAGCAAA GGAGCTGGCG 
TTGCAGCCCA AAGATGACAT CGTAGATAGA GCAAAAATGG AGGATACTTT GAAGAGGAGA 
TTTTTCTACG ATCAAGCTTT TGCTATTTAT GGAGGTGTCA GTGGTTTGTA TGACTTTGGG 
CCAGTGGGAT GTGCTTTGAA GAACAATATC ATCCAGACCT GGAGGCAGCA CTTTATCCAA 
GAGGAACAGA TCCTGGAAAT TGACTGCACC ATGCTCACCC CCGAGCCAGT TTTAAAGACC 
TCTGGCCATG TAGACAAATT TGCTGACTTC ATGGTGAAGG ACGTGAAGAA CGGAGAGTGC 
TTCCGCGCGG ACCACCTGTT GAAAGCTCAT TTACAGAAAC TGATGTCAGA TAAGAAGTGC 
TCTGCTGAGA AGAAATCAGA GATGGAAAGT GTCTTGGCCC AGCTTGATAA CTATGGACAA 
CAAGAACTGG CGGATCTTTT TGTGAACTAT AATGTAAAAT CTCCCACCAC TGGCAATGAC 
CTGTCCCCTC CGGTACCTTT TAACTTAATG TTCCAGACCT TCATTGGGCC TGGAGGAAAC 
ATGCCTGGAT ATCTGAGACC AGAAACTGCA CAGGGAATTT TCCTAAATTT CAAACGACTT 
TTGGAATTCA ACCAAGGGAA GTTGCCTTTT GCTGCTGCAC AGATTGGAAA CTCCTTTAGA 
AATGAGATCT CGCCCCGGTC TGGACTGATC CGAGTCAGGG AATTCACAAT GGCAGAGATC 
GAGCACTTTG TAGATCCCAC TGAGAAAGAC CATCCCAAGT TCCCGAGCGT GGCCGACCTC 
TACCTTTATT TGTACTCTGC CAAAGCCCAG GTCACTGGAC AGTCTGCTCG CAAGATGCGT 
CTGGGAGATG CTGTTGAACA GGGTGTGATT AACAACTCAG TATTAGGCTA TTTTATTGGC 
CGCATCTACC TCTACCTCAC GAAGGTTGGA ATATCTCCTG ATAAACTCCG CTTCCGACAG 
CATATGGAGA ATGAGATGGC CCATTATGCC TGCGACTGCT GGGATGCTGA GTCCAAAACG 
TCCTATGGCT GGATCGAGAT TGTTGGATGT GCTGACCGAT CCTGCTATGA CCTCTCCTGT 
CATGCTCGAG CCACCAAAGT CCCGCTGGTG GCTGAGAAGC CCCTGAAAGA GCCCAAAACA 
GTTAACGTTG TACAGTTTGA GCCCAACAAG GGAGCCGTGG GCAAGGCGTA CAAGAAAGAT 
GCAAAGCTGG TGCTGGAGTA CCTCGGCGCC TGCGATGAGT GCTACATTAC CGAGATGGAG 
CTGCTGCTGA GCGAGAAAGG GGAATTCACT ATTGAAACTG AAGGAAAAAC ATTTCAGTTA 
ACAAAAGACA TGGTCAGTGT GAAGAGATTC CAGAAAACAC TACACGTGGA AGAAGTTGTT 
CCGAGTGTAA TTGAGCCCTC TTTTGGCCTG GGCAGGATCA TGTATACCAT ATTGGAACAT 
ACATTCCACG TCCGGGAGGG AGATGAGCAG AGAACGTTCT TCAGTTTCCC TGCTGTGGTT 
GCTCCATTCA AATGTTCTGT CCTTCCACTG AGCCAAAACC AAGAGTTTAT GCCATTTGTC 
AAGGAATTAT CGGAAGCTCT GACTCGAAAT GGCGTGTCTC ATAAAGTTGA CGATTCCTCT 
GGGTCTATTG GAAGGCGCTA TGCAAGAACT GATGAGATTG GCGTGGCGTT TGGCATCACC 
ATTGACTTTG ATACAGTGAA CAAGACCCCC CACACTGCAA CTCTGAGGGA CCGAGACTCC 
ATGAGACAGA TAAGGGCAGA GGTCTCTGAA CTGCCCAGTG TGGTCCGTGA TCTGGCCAAT 
GGCAACATCA CTTGGGCTGA TGTGGAGGCC AGGTACCCCC TCTTTGAAGG GCAAGAGACT 
GGCAAGAAGG AGACAGTGGA AGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001258068.1
CDS	72..2258
Translation	MPCLLPTLLRATRAALLLQSPRVVAAPASQRLLSAPAQPAASPSSMDSAEELLAPLRLAVRQQGDFVRKLKEDKAPQVDVDRAVAELKARKRVLEAKELALQPKDDIVDRAKMEDTLKRRFFYDQAFAIYGGVSGLYDFGPVGCALKNNIIQTWRQHFIQEEQILEIDCTMLTPEPVLKTSGHVDKFADFMVKDVKNGECFRADHLLKAHLQKLMSDKKCSAEKKSEMESVLAQLDNYGQQELADLFVNYNVKSPTTGNDLSPPVPFNLMFQTFIGPGGNMPGYLRPETAQGIFLNFKRLLEFNQGKLPFAAAQIGNSFRNEISPRSGLIRVREFTMAEIEHFVDPTEKDHPKFPSVADLYLYLYSAKAQVTGQSARKMRLGDAVEQGVINNSVLGYFIGRIYLYLTKVGISPDKLRFRQHMENEMAHYACDCWDAESKTSYGWIEIVGCADRSCYDLSCHARATKVPLVAEKPLKEPKTVNVVQFEPNKGAVGKAYKKDAKLVLEYLGACDECYITEMELLLSEKGEFTIETEGKTFQLTKDMVSVKRFQKTLHVEEVVPSVIEPSFGLGRIMYTILEHTFHVREGDEQRTFFSFPAVVAPFKCSVLPLSQNQEFMPFVKELSEALTRNGVSHKVDDSSGSIGRRYARTDEIGVAFGITIDFDTVNKTPHTATLRDRDSMRQIRAEVSELPSVVRDLANGNITWADVEARYPLFEGQETGKKETVEE

Target ORF information:

RefSeq Version	NM_001271139.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus glycyl-tRNA synthetase (Gars), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001271139.2

ORF Insert Sequence:

ATGCCCTGTC TGCTCCCCAC GCTGCTCAGA GCCACCCGCG CCGCGCTGCT CCTACAGTCG 
CCCCGAGTGG TCGCAGCGCC CGCGTCTCAG CGGCTCCTCA GCGCTCCCGC GCAGCCCGCC 
GCCTCTCCGA GCAGCATGGA CAGCGCCGAG GAGCTGCTAG CCCCATTGCG GCTAGCTGTG 
CGCCAGCAGG GAGACTTTGT ACGGAAGTTA AAAGAAGATA AAGCCCCACA AGTGGATGTG 
GACAGAGCAG TAGCTGAGCT CAAGGCCCGG AAGAGGGTTC TGGAAGCAAA GGAGCTGGCG 
TTGCAGCCCA AAGATGACAT CGTAGATAGA GCAAAAATGG AGGATACTTT GAAGAGGAGA 
TTTTTCTACG ATCAAGCTTT TGCTATTTAT GGAGGTGTCA GTGGTTTGTA TGACTTTGGG 
CCAGTGGGAT GTGCTTTGAA GAACAATATC ATCCAGACCT GGAGGCAGCA CTTTATCCAA 
GAGGAACAGA TCCTGGAAAT TGACTGCACC ATGCTCACCC CCGAGCCAGT TTTAAAGACC 
TCTGGCCATG TAGACAAATT TGCTGACTTC ATGGTGAAGG ACGTGAAGAA CGGAGAGTGC 
TTCCGCGCGG ACCACCTGTT GAAAGCTCAT TTACAGAAAC TGATGTCAGA TAAGAAGTGC 
TCTGCTGAGA AGAAATCAGA GATGGAAAGT GTCTTGGCCC AGCTTGATAA CTATGGACAA 
CAAGAACTGG CGGATCTTTT TGTGAACTAT AATGTAAAAT CTCCCACCAC TGGCAATGAC 
CTGTCCCCTC CGGTACCTTT TAACTTAATG TTCCAGACCT TCATTGGGCC TGGAGGAAAC 
ATGCCTGGAT ATCTGAGACC AGAAACTGCA CAGGGAATTT TCCTAAATTT CAAACGACTT 
TTGGAATTCA ACCAAGGGAA GTTGCCTTTT GCTGCTGCAC AGATTGGAAA CTCCTTTAGA 
AATGAGATCT CGCCCCGGTC TGGACTGATC CGAGTCAGGG AATTCACAAT GGCAGAGATC 
GAGCACTTTG TAGATCCCAC TGAGAAAGAC CATCCCAAGT TCCCGAGCGT GGCCGACCTC 
TACCTTTATT TGTACTCTGC CAAAGCCCAG GTCACTGGAC AGTCTGCTCG CAAGATGCGT 
CTGGGAGATG CTGTTGAACA GGGTGTGATT AACAACTCAG TATTAGGCTA TTTTATTGGC 
CGCATCTACC TCTACCTCAC GAAGGTTGGA ATATCTCCTG ATAAACTCCG CTTCCGACAG 
CATATGGAGA ATGAGATGGC CCATTATGCC TGCGACTGCT GGGATGCTGA GTCCAAAACG 
TCCTATGGCT GGATCGAGAT TGTTGGATGT GCTGACCGAT CCTGCTATGA CCTCTCCTGT 
CATGCTCGAG CCACCAAAGT CCCGCTGGTG GCTGAGAAGC CCCTGAAAGA GCCCAAAACA 
GTTAACGTTG TACAGTTTGA GCCCAACAAG GGAGCCGTGG GCAAGGCGTA CAAGAAAGAT 
GCAAAGCTGG TGCTGGAGTA CCTCGGCGCC TGCGATGAGT GCTACATTAC CGAGATGGAG 
CTGCTGCTGA GCGAGAAAGG GGAATTCACT ATTGAAACTG AAGGAAAAAC ATTTCAGTTA 
ACAAAAGACA TGGTCAGTGT GAAGAGATTC CAGAAAACAC TACACGTGGA AGAAGTTGTT 
CCGAGTGTAA TTGAGCCCTC TTTTGGCCTG GGCAGGATCA TGTATACCAT ATTGGAACAT 
ACATTCCACG TCCGGGAGGG AGATGAGCAG AGAACGTTCT TCAGTTTCCC TGCTGTGGTT 
GCTCCATTCA AATGTTCTGT CCTTCCACTG AGCCAAAACC AAGAGTTTAT GCCATTTGTC 
AAGGAATTAT CGGAAGCTCT GACTCGAAAT GGCGTGTCTC ATAAAGTTGA CGATTCCTCT 
GGGTCTATTG GAAGGCGCTA TGCAAGAACT GATGAGATTG GCGTGGCGTT TGGCATCACC 
ATTGACTTTG ATACAGTGAA CAAGACCCCC CACACTGCAA CTCTGAGGGA CCGAGACTCC 
ATGAGACAGA TAAGGGCAGA GGTCTCTGAA CTGCCCAGTG TGGTCCGTGA TCTGGCCAAT 
GGCAACATCA CTTGGGCTGA TGTGGAGGCC AGGTACCCCC TCTTTGAAGG GCAAGAGACT 
GGCAAGAAGG AGACAGTGGA AGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Compound heterozygosity for loss-of-function GARS variants results in a multisystem developmental syndrome that includes severe growth retardation.
Human mutation38(10)1412-1420(2017 Oct)
Oprescu SN,Chepa-Lotrea X,Takase R,Golas G,Markello TC,Adams DR,Toro C,Gropman AL,Hou YM,Malicdan MCV,Gahl WA,Tifft CJ,Antonellis A

CMT2D neuropathy is linked to the neomorphic binding activity of glycyl-tRNA synthetase.
Nature526(7575)710-4(2015 Oct)
He W,Bai G,Zhou H,Wei N,White NM,Lauer J,Liu H,Shi Y,Dumitru CD,Lettieri K,Shubayev V,Jordanova A,Guergueltcheva V,Griffin PR,Burgess RW,Pfaff SL,Yang XL

Crystal structures and biochemical analyses suggest a unique mechanism and role for human glycyl-tRNA synthetase in Ap4A homeostasis.
The Journal of biological chemistry284(42)28968-76(2009 Oct)
Guo RT,Chong YE,Guo M,Yang XL

An ENU-induced mutation in mouse glycyl-tRNA synthetase (GARS) causes peripheral sensory and motor phenotypes creating a model of Charcot-Marie-Tooth type 2D peripheral neuropathy.
Disease models & mechanisms2(7-8)359-73(2009 Jul-Aug)
Achilli F,Bros-Facer V,Williams HP,Banks GT,AlQatari M,Chia R,Tucci V,Groves M,Nickols CD,Seburn KL,Kendall R,Cader MZ,Talbot K,van Minnen J,Burgess RW,Brandner S,Martin JE,Koltzenburg M,Greensmith L,Nolan PM,Fisher EM

Charcot-Marie-Tooth disease-associated mutant tRNA synthetases linked to altered dimer interface and neurite distribution defect.
Proceedings of the National Academy of Sciences of the United States of America104(27)11239-44(2007 Jul)
Nangle LA,Zhang W,Xie W,Yang XL,Schimmel P

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Gars cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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