Cst8 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cst8
Entrez Gene ID	29679
Full Name	cystatin 8
General protein information	Preferred Names cystatin 8 Names cystatin-8 cystatin 8 (cystatin-related epididymal specific) cystatin 8 (cystatin-related epididymal spermatogenic) cystatin-related epididymal spermatogenic protein
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 3 Map Location: 3q41
Summary	may act as a cysteine protease inhibitor in the male reproductive tract [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_019258.1	NP_062131.1	cystatin-8 precursor
XM_017591666.1	XP_017447155.1	cystatin-8 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	CST8	NP_001268659.1
Bos taurus (cattle)	CST8	XP_005214475.1
	CST8	XP_001096851.1
Canis lupus familiaris (dog)	LOC485735	XP_005634761.1
Rattus norvegicus (Norway rat)	Cst8	NP_062131.1
Pan troglodytes (chimpanzee)	CST8	XP_003953737.1
Mus musculus (house mouse)	Cst8	NP_034108.1

Gene Ontology
Bibliography

Related articles in PubMed

Structure, alternative splicing and chromosomal localization of the cystatin-related epididymal spermatogenic gene.
Cornwall GA, Hsia N, Sutton HG
The Biochemical journal340 ( Pt 1)85-93(1999 May)

Transient appearance of CRES protein during spermatogenesis and caput epididymal sperm maturation.
Cornwall GA, Hann SR
Molecular reproduction and development41(1)37-46(1995 May)

The CRES gene: a unique testis-regulated gene related to the cystatin family is highly restricted in its expression to the proximal region of the mouse epididymis.
Cornwall GA, Orgebin-Crist MC, Hann SR
Molecular endocrinology (Baltimore, Md.)6(10)1653-64(1992 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Cst8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cst8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa04193	NM_019258.1 Latest version!	Rattus norvegicus cystatin 8 (Cst8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
ORa60370	XM_017591666.1 Latest version!	Rattus norvegicus cystatin 8 (Cst8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cst8 ( NM_019258.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_062131.1 Rattus norvegicus cystatin 8 (Cst8), mRNA.

Price & Availability↑

CloneID	ORa04193
Clone ID Related Accession (Same CDS sequence)	NM_019258.1
Accession Version	NM_019258.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 429bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-15
Organism	Rattus norvegicus(Norway rat)
Product	cystatin-8 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF090692.1. ##Evidence-Data-START## Transcript exon combination :: AF090692.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689604, SAMN02642677 [ECO:0000348] ##Evidence-Data-END##

ATGACAAAAC CCTTGTTGCT CTCCTTGATC TTCTTCATCA TTCCAGCGGC CCTGGCAGTT 
GATGTGGATC AGTCTAAGAA TGAAGTGAAG GCACAGAGGT ACTTTGGATC CATTAGTATC 
TCCAATGCCA ATGTGAAGCA ATGTGTTTGG TTTGCCATGA AAGAATACAA CAAAGGAAGT 
GAGGACAAAT ACCTCTTCCT TCTGGACAAG ACACTCCATG CCACACTTCA GATCACAGAC 
CGCATGGAAT ACCACATTGA TGTTCAGATC TCCCGCAGCA ATTGCAGAAA ACCTTTAAAC 
AATACTGAAA ACTGCATCCC TCAAAAAAAT CCCAAACTGG AAAAGAAATT AAGTTGCAGC 
TTTCTGGTAG GAGCACTTCC CTGGAATGGT GAGTTCGACC TGTTGAGCAA AGAATGCAAA 
GATGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_062131.1
CDS	195..623
Translation	MTKPLLLSLIFFIIPAALAVDVDQSKNEVKAQRYFGSISISNANVKQCVWFAMKEYNKGSEDKYLFLLDKTLHATLQITDRMEYHIDVQISRSNCRKPLNNTENCIPQKNPKLEKKLSCSFLVGALPWNGEFDLLSKECKDV

Target ORF information:

RefSeq Version	NM_019258.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus cystatin 8 (Cst8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019258.1

ORF Insert Sequence:

ATGACAAAAC CCTTGTTGCT CTCCTTGATC TTCTTCATCA TTCCAGCGGC CCTGGCAGTT 
GATGTGGATC AGTCTAAGAA TGAAGTGAAG GCACAGAGGT ACTTTGGATC CATTAGTATC 
TCCAATGCCA ATGTGAAGCA ATGTGTTTGG TTTGCCATGA AAGAATACAA CAAAGGAAGT 
GAGGACAAAT ACCTCTTCCT TCTGGACAAG ACACTCCATG CCACACTTCA GATCACAGAC 
CGCATGGAAT ACCACATTGA TGTTCAGATC TCCCGCAGCA ATTGCAGAAA ACCTTTAAAC 
AATACTGAAA ACTGCATCCC TCAAAAAAAT CCCAAACTGG AAAAGAAATT AAGTTGCAGC 
TTTCTGGTAG GAGCACTTCC CTGGAATGGT GAGTTCGACC TGTTGAGCAA AGAATGCAAA 
GATGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cst8 ( XM_017591666.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017447155.1 Rattus norvegicus cystatin 8 (Cst8), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa60370
Clone ID Related Accession (Same CDS sequence)	XM_017591666.1
Accession Version	XM_017591666.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 396bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	cystatin-8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAAAAC CCTTGTTGCT CTCCTTGATC TTCTTCATCA TTCCAGCGGC CCTGGCAGTT 
GATGTGGATC AGTCTAAGAA TGAAGTGAAG GCACAGAGGT ACTTTGGATC CATTAGTATC 
TCCAATGCCA ATGTGAAGCA ATGTGTTTGG TTTGCCATGA AAGAATACAA CAAAGGAAGT 
GAGGACAAAT ACCTCTTCCT TCTGGACAAG ACACTCCATG CCACACTTCA GATCACAGAC 
CGCATGGAAT ACCACATTGA TGTTCAGATC TCCCGCAGCA ATTGCAGAAA ACCTTTAAAC 
AATACTGAAA ACTGCATCCC TCAAAAAAAT CCCAAACTGG AAAAGAAATT AAGTTGCAGC 
TTTCTGGTAG GAGCACTTCC CTGGAATGCT TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017447155.1
CDS	236..631
Translation	MTKPLLLSLIFFIIPAALAVDVDQSKNEVKAQRYFGSISISNANVKQCVWFAMKEYNKGSEDKYLFLLDKTLHATLQITDRMEYHIDVQISRSNCRKPLNNTENCIPQKNPKLEKKLSCSFLVGALPWNAS

Target ORF information:

RefSeq Version	XM_017591666.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus cystatin 8 (Cst8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017591666.1

ORF Insert Sequence:

ATGACAAAAC CCTTGTTGCT CTCCTTGATC TTCTTCATCA TTCCAGCGGC CCTGGCAGTT 
GATGTGGATC AGTCTAAGAA TGAAGTGAAG GCACAGAGGT ACTTTGGATC CATTAGTATC 
TCCAATGCCA ATGTGAAGCA ATGTGTTTGG TTTGCCATGA AAGAATACAA CAAAGGAAGT 
GAGGACAAAT ACCTCTTCCT TCTGGACAAG ACACTCCATG CCACACTTCA GATCACAGAC 
CGCATGGAAT ACCACATTGA TGTTCAGATC TCCCGCAGCA ATTGCAGAAA ACCTTTAAAC 
AATACTGAAA ACTGCATCCC TCAAAAAAAT CCCAAACTGG AAAAGAAATT AAGTTGCAGC 
TTTCTGGTAG GAGCACTTCC CTGGAATGCT TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Structure, alternative splicing and chromosomal localization of the cystatin-related epididymal spermatogenic gene.
The Biochemical journal340 ( Pt 1)85-93(1999 May)
Cornwall GA,Hsia N,Sutton HG

Transient appearance of CRES protein during spermatogenesis and caput epididymal sperm maturation.
Molecular reproduction and development41(1)37-46(1995 May)
Cornwall GA,Hann SR

The CRES gene: a unique testis-regulated gene related to the cystatin family is highly restricted in its expression to the proximal region of the mouse epididymis.
Molecular endocrinology (Baltimore, Md.)6(10)1653-64(1992 Oct)
Cornwall GA,Orgebin-Crist MC,Hann SR