Papss1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Papss1
Entrez Gene ID	295443
Full Name	3'-phosphoadenosine 5'-phosphosulfate synthase 1
General protein information	Preferred Names 3'-phosphoadenosine 5'-phosphosulfate synthase 1 Names bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 2 Map Location: 2q43

mRNA and Protein(s)

mRNA	Protein	Name
NM_001106471.1	NP_001099941.1	bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	PAPSS1	XP_517384.4
Saccharomyces cerevisiae (baker's yeast)	MET14	NP_012925.3
Magnaporthe oryzae (rice blast fungus)	MGG_06348	XP_003717233.1
Drosophila melanogaster (fruit fly)	Papss	NP_001262072.1
Neurospora crassa	NCU09896	XP_958621.1
Homo sapiens (human)	PAPSS1	NP_005434.4
Rattus norvegicus (Norway rat)	Papss1	NP_001099941.1
Kluyveromyces lactis	KLLA0B13321g	XP_452124.1
Schizosaccharomyces pombe (fission yeast)	met14	NP_594718.1
Xenopus tropicalis (tropical clawed frog)	papss1	NP_001006743.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001256	XP_003435789.1
	PAPSS1	XP_001085840.2
Canis lupus familiaris (dog)	PAPSS1	XP_851070.2
Bos taurus (cattle)	PAPSS1	NP_001029382.1
Mus musculus (house mouse)	Papss1	NP_035993.1
Gallus gallus (chicken)	PAPSS1	XP_420493.3
Danio rerio (zebrafish)	papss1	NP_001122171.1

Gene Ontology
Bibliography

Related articles in PubMed

Ethanol sulfation by the human cytosolic sulfotransferases: a systematic analysis.
Kurogi K, Davidson G, Mohammed YI, Williams FE, Liu MY, Sakakibara Y, Suiko M, Liu MC
Biological & pharmaceutical bulletin35(12)2180-5(2012)

Expression, purification and crystallization of human 3'-phosphoadenosine-5'-phosphosulfate synthetase 1.
Harjes S, Scheidig A, Bayer P
Acta crystallographica. Section D, Biological crystallography60(Pt 2)350-2(2004 Feb)

Identification and functional characterization of the novel BM-motif in the murine phosphoadenosine phosphosulfate (PAPS) synthetase.
Singh B, Schwartz NB
The Journal of biological chemistry278(1)71-5(2003 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Papss1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Papss1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa04750	NM_001106471.1 Latest version!	Rattus norvegicus 3'-phosphoadenosine 5'-phosphosulfate synthase 1 (Papss1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Papss1 ( NM_001106471.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001099941.1 Rattus norvegicus 3'-phosphoadenosine 5'-phosphosulfate synthase 1 (Papss1), mRNA.

Price & Availability↑

CloneID	ORa04750
Clone ID Related Accession (Same CDS sequence)	NM_001106471.1
Accession Version	NM_001106471.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1746bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-27
Organism	Rattus norvegicus(Norway rat)
Product	bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473952.1. On or before Oct 4, 2007 this sequence version replaced XM_215701.4, XM_001077181.1. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGAGATTC CTGGGAGTCT GTGCAAGAAA GTCAAGCTGA GCAATAACGC ACAGAACTGG 
GGGATGCAGA GAGCAACCAA CGTCACCTAT CAAGCACACC ATGTCAGCCG GAATAAGAGA 
GGTCAGGTGG TGGGGACCAG AGGTGGCTTC CGTGGTTGCA CAGTTTGGCT GACAGGATTG 
TCTGGAGCAG GGAAGACGAC CGTGAGCATG GCTCTGGAGG AGTACCTGGT TTGCCATGGC 
ATTCCGTGCT ACACTTTGGA TGGCGACAAC ATCCGTCAAG GCCTCAATAA GAACCTTGGC 
TTCAGTCCTG AGGACAGAGA GGAGAATGTT CGCCGCATAG CAGAGGTGGC AAAGCTGTTC 
GCAGACGCTG GCCTCGTGTG CATCACAAGC TTCATATCGC CCTACACGCA GGTAAGGCAA 
GGCTTCACTG GGATCGACTC TGAGTATGAG AAACCAGAGG CCCCGGAACT GGTGCTGAAA 
ACGGATTCTT GTGATGTCAA CGACTGTGTC CAGCAGGTCG TGGAGCTTCT GCAGGAACGG 
GACATTGTCC CTGTGGATGC CTCCTATGAG GTGAAAGAGC TGTATGTGCC GGAGAACAAA 
CTTCACTTGG CCAAAACTGA TGCAGAAGCC TTACCAGCAC TGAAAATCAA CAAAGTGGAC 
ATGCAGTGGG TGCAGGTTCT GGCAGAAGGC TGGGCGACTC CTCTGAACGG CTTCATGAGG 
GAGAGGGAAT ACTTGCAGTG CCTTCATTTT GACTGTCTTC TGGATGGAGG CGTCATCAAC 
TTATCCGTGC CTATAGTCCT GACAGCTACG CAGGAGGACA AGGAGAGGCT GGACGGCTGC 
ACAGCGTTCG CTCTGGTGTA TGAGGGCCGC CGTGTGGCCA TCCTCCGGAA TCCGGAATTT 
TTTGAGCACC GGAAAGAGGA GCGTTGCGCC CGACAGTGGG GAACAACGTG CAGGAGCCAC 
CCCTACATCA AGATGATTCT GGAACAAGGG GATTGGCTGA TTGGAGGAGA TCTTCAAGTC 
CTGGACCGGA TTTACTGGAA CGATGGCCTT GATCAGTACC GTCTTACCCC TGCGGAGCTC 
AAGCAGAAGT TTAAAGATAT GAATGCTGAT GCTGTCTTTG CGTTTCAGTT GCGCAACCCA 
GTGCACAATG GACACGCTCT GCTAATGCAG GACACCCACA AGCAGCTTCT AGAGAGGGGC 
TACCGGCGTC CAGTCCTCCT CCTCCATCCG CTTGGTGGCT GGACGAAGGA TGATGATGTC 
CCTCTGATGT GGCGTATGAA GCAACATGCT GCAGTGCTGG AGGAGGGCAT CCTGAATCCT 
GAAACAACTG TGGTGGCCAT TTTCCCATCT CCTATGATGT ATGCTGGGCC GACCGAGGTC 
CAGTGGCACT GCAGGGCTCG GATGGTGGCA GGGGCCAATT TTTATATTGT TGGACGAGAC 
CCTGCTGGCA TGCCTCATCC GGAGACAGGG AAGGACCTCT ATGAGCCAAC CCACGGTGCC 
AAAGTGCTGA CGATGGCCCC CGGCCTGATC ACCTTGGAAA TCGTTCCCTT CCGAGTCGCA 
GCTTACAACA AGAAGAAGAA GCGGATGGAC TACTATGACT CGGATCACCA CGAAGACTTC 
GAATTTATTT CAGGAACCAG GATGCGCAAA CTGGCACGTG AAGGCCAGAA ACCTCCAGAG 
GGCTTCATGG CCCCCAAGGC GTGGACTGTG CTGGTGGAGT ACTACAAGTC CTTAGAGAAA 
GCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001099941.1
CDS	64..1809
Translation	MEIPGSLCKKVKLSNNAQNWGMQRATNVTYQAHHVSRNKRGQVVGTRGGFRGCTVWLTGLSGAGKTTVSMALEEYLVCHGIPCYTLDGDNIRQGLNKNLGFSPEDREENVRRIAEVAKLFADAGLVCITSFISPYTQVRQGFTGIDSEYEKPEAPELVLKTDSCDVNDCVQQVVELLQERDIVPVDASYEVKELYVPENKLHLAKTDAEALPALKINKVDMQWVQVLAEGWATPLNGFMREREYLQCLHFDCLLDGGVINLSVPIVLTATQEDKERLDGCTAFALVYEGRRVAILRNPEFFEHRKEERCARQWGTTCRSHPYIKMILEQGDWLIGGDLQVLDRIYWNDGLDQYRLTPAELKQKFKDMNADAVFAFQLRNPVHNGHALLMQDTHKQLLERGYRRPVLLLHPLGGWTKDDDVPLMWRMKQHAAVLEEGILNPETTVVAIFPSPMMYAGPTEVQWHCRARMVAGANFYIVGRDPAGMPHPETGKDLYEPTHGAKVLTMAPGLITLEIVPFRVAAYNKKKKRMDYYDSDHHEDFEFISGTRMRKLAREGQKPPEGFMAPKAWTVLVEYYKSLEKA

Target ORF information:

RefSeq Version	NM_001106471.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus 3'-phosphoadenosine 5'-phosphosulfate synthase 1 (Papss1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001106471.1

ORF Insert Sequence:

ATGGAGATTC CTGGGAGTCT GTGCAAGAAA GTCAAGCTGA GCAATAACGC ACAGAACTGG 
GGGATGCAGA GAGCAACCAA CGTCACCTAT CAAGCACACC ATGTCAGCCG GAATAAGAGA 
GGTCAGGTGG TGGGGACCAG AGGTGGCTTC CGTGGTTGCA CAGTTTGGCT GACAGGATTG 
TCTGGAGCAG GGAAGACGAC CGTGAGCATG GCTCTGGAGG AGTACCTGGT TTGCCATGGC 
ATTCCGTGCT ACACTTTGGA TGGCGACAAC ATCCGTCAAG GCCTCAATAA GAACCTTGGC 
TTCAGTCCTG AGGACAGAGA GGAGAATGTT CGCCGCATAG CAGAGGTGGC AAAGCTGTTC 
GCAGACGCTG GCCTCGTGTG CATCACAAGC TTCATATCGC CCTACACGCA GGTAAGGCAA 
GGCTTCACTG GGATCGACTC TGAGTATGAG AAACCAGAGG CCCCGGAACT GGTGCTGAAA 
ACGGATTCTT GTGATGTCAA CGACTGTGTC CAGCAGGTCG TGGAGCTTCT GCAGGAACGG 
GACATTGTCC CTGTGGATGC CTCCTATGAG GTGAAAGAGC TGTATGTGCC GGAGAACAAA 
CTTCACTTGG CCAAAACTGA TGCAGAAGCC TTACCAGCAC TGAAAATCAA CAAAGTGGAC 
ATGCAGTGGG TGCAGGTTCT GGCAGAAGGC TGGGCGACTC CTCTGAACGG CTTCATGAGG 
GAGAGGGAAT ACTTGCAGTG CCTTCATTTT GACTGTCTTC TGGATGGAGG CGTCATCAAC 
TTATCCGTGC CTATAGTCCT GACAGCTACG CAGGAGGACA AGGAGAGGCT GGACGGCTGC 
ACAGCGTTCG CTCTGGTGTA TGAGGGCCGC CGTGTGGCCA TCCTCCGGAA TCCGGAATTT 
TTTGAGCACC GGAAAGAGGA GCGTTGCGCC CGACAGTGGG GAACAACGTG CAGGAGCCAC 
CCCTACATCA AGATGATTCT GGAACAAGGG GATTGGCTGA TTGGAGGAGA TCTTCAAGTC 
CTGGACCGGA TTTACTGGAA CGATGGCCTT GATCAGTACC GTCTTACCCC TGCGGAGCTC 
AAGCAGAAGT TTAAAGATAT GAATGCTGAT GCTGTCTTTG CGTTTCAGTT GCGCAACCCA 
GTGCACAATG GACACGCTCT GCTAATGCAG GACACCCACA AGCAGCTTCT AGAGAGGGGC 
TACCGGCGTC CAGTCCTCCT CCTCCATCCG CTTGGTGGCT GGACGAAGGA TGATGATGTC 
CCTCTGATGT GGCGTATGAA GCAACATGCT GCAGTGCTGG AGGAGGGCAT CCTGAATCCT 
GAAACAACTG TGGTGGCCAT TTTCCCATCT CCTATGATGT ATGCTGGGCC GACCGAGGTC 
CAGTGGCACT GCAGGGCTCG GATGGTGGCA GGGGCCAATT TTTATATTGT TGGACGAGAC 
CCTGCTGGCA TGCCTCATCC GGAGACAGGG AAGGACCTCT ATGAGCCAAC CCACGGTGCC 
AAAGTGCTGA CGATGGCCCC CGGCCTGATC ACCTTGGAAA TCGTTCCCTT CCGAGTCGCA 
GCTTACAACA AGAAGAAGAA GCGGATGGAC TACTATGACT CGGATCACCA CGAAGACTTC 
GAATTTATTT CAGGAACCAG GATGCGCAAA CTGGCACGTG AAGGCCAGAA ACCTCCAGAG 
GGCTTCATGG CCCCCAAGGC GTGGACTGTG CTGGTGGAGT ACTACAAGTC CTTAGAGAAA 
GCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Ethanol sulfation by the human cytosolic sulfotransferases: a systematic analysis.
Biological & pharmaceutical bulletin35(12)2180-5(2012)
Kurogi K,Davidson G,Mohammed YI,Williams FE,Liu MY,Sakakibara Y,Suiko M,Liu MC

Expression, purification and crystallization of human 3'-phosphoadenosine-5'-phosphosulfate synthetase 1.
Acta crystallographica. Section D, Biological crystallography60(Pt 2)350-2(2004 Feb)
Harjes S,Scheidig A,Bayer P

Identification and functional characterization of the novel BM-motif in the murine phosphoadenosine phosphosulfate (PAPS) synthetase.
The Journal of biological chemistry278(1)71-5(2003 Jan)
Singh B,Schwartz NB