Timp2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Timp2
Entrez Gene ID	29543
Full Name	TIMP metallopeptidase inhibitor 2
General protein information	Preferred Names TIMP metallopeptidase inhibitor 2 Names metalloproteinase inhibitor 2 TIMP-2 tissue inhibitor of metalloproteinase 2 tissue inhibitor of metalloproteinases 2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q32.3
Summary	acts as an inhibitor of metallopeptidase activity; may play a role in tissue remodeling during spermatogenesis [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_021989.2	NP_068824.1	metalloproteinase inhibitor 2 precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	TIMP2	NP_003246.1
Bos taurus (cattle)	TIMP2	NP_776897.2
Pan troglodytes (chimpanzee)	TIMP2	XP_001146829.2
	TIMP2	XP_001106657.2
Gallus gallus (chicken)	TIMP2	NP_989629.1
Danio rerio (zebrafish)	timp2a	NP_878294.1
Xenopus tropicalis (tropical clawed frog)	timp2	NP_001015760.1
Canis lupus familiaris (dog)	TIMP2	NP_001003082.1
Mus musculus (house mouse)	Timp2	NP_035724.2
Rattus norvegicus (Norway rat)	Timp2	NP_068824.1

Gene Ontology
Bibliography

Related articles in PubMed

Dynamics of Distribution of Capillaries with Matrix Metalloproteinase-2 and Its Tissue Inhibitor in Rat Brain during Development of Experimental Hypertension.
Chertok VM, Chertok AG, Zakharchuk NV, Nevzorova VA
Bulletin of experimental biology and medicine164(3)397-401(2018 Jan)

Copper-induced reduction in myocardial fibrosis is associated with increased matrix metalloproteins in a rat model of cardiac hypertrophy.
Liu Y, Xiao Y, Liu J, Feng L, Kang YJ
Metallomics : integrated biometal science(2018 Jan)

Correlation between Tissue Characterization and Dynamic Expression of Matrix Metalloproteinase-2 and Its Tissue Inhibitor in Conjunctival Filtering Bleb of Rats.
Wang L, Wang DB, Liu MY, Yao RY
BioMed research international20171054129(2017)

Artesunate modulates expression of matrix metalloproteinases and their inhibitors as well as collagen-IV to attenuate pulmonary fibrosis in rats.
Wang Y, Huang G, Mo B, Wang C
Genetics and molecular research : GMR15(2)(2016 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These findings suggested that artesunate attenuates alveolitis and pulmonary fibrosis by regulating expression of collagen-IV, TIMP-1 and 2, as well as MMP-2 and -9, to reduce ECM accumulation.
Title: Artesunate modulates expression of matrix metalloproteinases and their inhibitors as well as collagen-IV to attenuate pulmonary fibrosis in rats.

Data suggest that amlodipine and Atorvastatin could improve ventricular remodeling in SHR rats through intervention with the imbalance of MMP-2/TIMP-2 and MMP-9/TIMP-1 system.
Title: Amlodipine and Atorvastatin Improved Hypertensive Cardiac Remodeling through Regulation of MMPs/TIMPs in SHR Rats.

Lung tissue from burn animals showed increased matrix metalloproteinase activity, decreased levels of TIMP-2, and no difference in levels of active MMP-9 in burn vs. control groups.
Title: Tissue inhibitor of metalloproteinase-2 inhibits burn-induced derangements and hyperpermeability in microvascular endothelial cells.

MMP-2 and MMP-9 activities and TIMP-1 and TIMP-2 expression were significantly reduced in the lateral prostatic lobe of the ethanol drinking animals.
Title: MMP-2 and MMP-9 activities and TIMP-1 and TIMP-2 expression in the prostatic tissue of two ethanol-preferring rat models.

decreased in diabetic skin
Title: The skin remodeling in type 1 diabetes and insulin resistance animal models.

The following Timp2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Timp2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa05346	NM_021989.2 Latest version!	Rattus norvegicus TIMP metallopeptidase inhibitor 2 (Timp2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Timp2 ( NM_021989.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_068824.1 Rattus norvegicus TIMP metallopeptidase inhibitor 2 (Timp2), mRNA.

Price & Availability↑

CloneID	ORa05346
Clone ID Related Accession (Same CDS sequence)	NM_021989.2
Accession Version	NM_021989.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 663bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Rattus norvegicus(Norway rat)
Product	metalloproteinase inhibitor 2 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AJ409332.1. On Jun 4, 2003 this sequence version replaced NM_021989.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ409332.1, BC084714.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGGCGCCG CGGCCCGCAG CCTCCGGCTG GCGCTCGGCC TCCTGCTGCT GGCCACGCTG 
CTGCGCCCGG CCGACGCCTG CAGCTGCTCC CCGGTGCACC CGCAACAGGC GTTTTGCAAT 
GCAGACGTAG TGATCAGGGC CAAAGCAGTG AGCGAGAAGG AGGTGGATTC CGGGAATGAC 
ATCTATGGCA ACCCCATCAA GAGGATTCAA TATGAGATCA AGCAGATAAA GATGTTCAAA 
GGACCTGACA AGGACATCGA ATTTATCTAC ACGGCCCCCT CCTCAGCAGT GTGCGGGGTC 
TCGCTGGACG TTGGAGGAAA GAAGGAATAT CTAATTGCAG GGAAGGCGGA AGGAGATGGC 
AAGATGCACA TTACCCTCTG TGACTTTATT GTGCCCTGGG ACACGCTTAG CATCACCCAG 
AAGAAGAGCC TAAACCACAG GTACCAGATG GGCTGCGAGT GCAAGATCAC ACGCTGCCCT 
ATGATCCCAT GCTACATCTC CTCCCCGGAT GAGTGCCTCT GGATGGACTG GGTCACAGAG 
AAGAGCATCA ATGGGCACCA GGCCAAGTTC TTTGCCTGCA TCAAGAGAAG TGATGGTTCT 
TGCGCGTGGT ACCGCGGGGC GGCACCCCCG AAGCAGGAGT TTCTTGACAT CGAGGACCCG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_068824.1
CDS	126..788
Translation	MGAAARSLRLALGLLLLATLLRPADACSCSPVHPQQAFCNADVVIRAKAVSEKEVDSGNDIYGNPIKRIQYEIKQIKMFKGPDKDIEFIYTAPSSAVCGVSLDVGGKKEYLIAGKAEGDGKMHITLCDFIVPWDTLSITQKKSLNHRYQMGCECKITRCPMIPCYISSPDECLWMDWVTEKSINGHQAKFFACIKRSDGSCAWYRGAAPPKQEFLDIEDP

Target ORF information:

RefSeq Version	NM_021989.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus TIMP metallopeptidase inhibitor 2 (Timp2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021989.2

ORF Insert Sequence:

ATGGGCGCCG CGGCCCGCAG CCTCCGGCTG GCGCTCGGCC TCCTGCTGCT GGCCACGCTG 
CTGCGCCCGG CCGACGCCTG CAGCTGCTCC CCGGTGCACC CGCAACAGGC GTTTTGCAAT 
GCAGACGTAG TGATCAGGGC CAAAGCAGTG AGCGAGAAGG AGGTGGATTC CGGGAATGAC 
ATCTATGGCA ACCCCATCAA GAGGATTCAA TATGAGATCA AGCAGATAAA GATGTTCAAA 
GGACCTGACA AGGACATCGA ATTTATCTAC ACGGCCCCCT CCTCAGCAGT GTGCGGGGTC 
TCGCTGGACG TTGGAGGAAA GAAGGAATAT CTAATTGCAG GGAAGGCGGA AGGAGATGGC 
AAGATGCACA TTACCCTCTG TGACTTTATT GTGCCCTGGG ACACGCTTAG CATCACCCAG 
AAGAAGAGCC TAAACCACAG GTACCAGATG GGCTGCGAGT GCAAGATCAC ACGCTGCCCT 
ATGATCCCAT GCTACATCTC CTCCCCGGAT GAGTGCCTCT GGATGGACTG GGTCACAGAG 
AAGAGCATCA ATGGGCACCA GGCCAAGTTC TTTGCCTGCA TCAAGAGAAG TGATGGTTCT 
TGCGCGTGGT ACCGCGGGGC GGCACCCCCG AAGCAGGAGT TTCTTGACAT CGAGGACCCG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Dynamics of Distribution of Capillaries with Matrix Metalloproteinase-2 and Its Tissue Inhibitor in Rat Brain during Development of Experimental Hypertension.
Bulletin of experimental biology and medicine164(3)397-401(2018 Jan)
Chertok VM,Chertok AG,Zakharchuk NV,Nevzorova VA

Copper-induced reduction in myocardial fibrosis is associated with increased matrix metalloproteins in a rat model of cardiac hypertrophy.
Metallomics : integrated biometal science(2018 Jan)
Liu Y,Xiao Y,Liu J,Feng L,Kang YJ

Correlation between Tissue Characterization and Dynamic Expression of Matrix Metalloproteinase-2 and Its Tissue Inhibitor in Conjunctival Filtering Bleb of Rats.
BioMed research international20171054129(2017)
Wang L,Wang DB,Liu MY,Yao RY

Artesunate modulates expression of matrix metalloproteinases and their inhibitors as well as collagen-IV to attenuate pulmonary fibrosis in rats.
Genetics and molecular research : GMR15(2)(2016 Jun)
Wang Y,Huang G,Mo B,Wang C