Pdx1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pdx1
Entrez Gene ID	29535
Full Name	pancreatic and duodenal homeobox 1
Synonyms	Idx1,Ipf1,Stf1
General protein information	Preferred Names pancreatic and duodenal homeobox 1 Names pancreas/duodenum homeobox protein 1 IDX-1 IPF-1 Insulin promoter factor 1 STF-1 insulin promotor factor 1 islet/duodenum homeobox-1 pancreatic and duodenal homeobox gene 1 somatostatin transactivating factor 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 12 Map Location: 12p11

mRNA and Protein(s)

mRNA	Protein	Name
NM_022852.3	NP_074043.3	pancreas/duodenum homeobox protein 1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	PDX1	NP_001179065.1
	PDX1	XP_001096758.1
Xenopus tropicalis (tropical clawed frog)	pdx1	XP_002934065.1
Canis lupus familiaris (dog)	PDX1	XP_543155.3
Mus musculus (house mouse)	Pdx1	NP_032840.1
Pan troglodytes (chimpanzee)	PDX1	NP_001074947.1
Danio rerio (zebrafish)	pdx1	NP_571518.2
Homo sapiens (human)	PDX1	NP_000200.1
Rattus norvegicus (Norway rat)	Pdx1	NP_074043.3
Gallus gallus (chicken)	PDX1	XP_001234636.2

Gene Ontology
Bibliography

Related articles in PubMed

The Synergy of Two Factors on Insulin Expression.
Luo Y, Xu Y, Wang ZY, Li X, Xing WB, Zhang TC
Cellular reprogramming(2018 Jan)

Sphallerocarpus gracilis polysaccharide protects pancreatic ?-cells via regulation of the bax/bcl-2, caspase-3, pdx-1 and insulin signalling pathways.
Guo J, Wang J, Song S, Liu Q, Huang Y, Xu Y, Wei Y, Zhang J
International journal of biological macromolecules93(Pt A)829-836(2016 Dec)

A Pdx-1-Regulated Soluble Factor Activates Rat and Human Islet Cell Proliferation.
Hayes HL, Zhang L, Becker TC, Haldeman JM, Stephens SB, Arlotto M, Moss LG, Newgard CB, Hohmeier HE
Molecular and cellular biology36(23)2918-2930(2016 Dec)

Regulation of postnatal pancreatic Pdx1 and downstream target genes after gestational exposure to protein restriction in rats.
Abuzgaia AM, Hardy DB, Arany E
Reproduction (Cambridge, England)149(3)293-303(2015 Mar)

Identification and differentiation of PDX1 ?-cell progenitors within the human pancreatic epithelium.
Seeberger KL, Anderson SJ, Ellis CE, Yeung TY, Korbutt GS
World journal of diabetes5(1)59-68(2014 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Sphallerocarpus gracilis polysaccharide protected pancreatic beta-cells from alloxan damage by several possible mechanisms, including: (1) repairing free radical damage; (2) reducing the apoptosis of pancreatic beta-cells by inhibiting the activities of caspase-3 and bax, and enhancing the activity of bcl-2; (3) stimulating insulin secretion and upregulating the pancreatic and duodenal homeobox 1 gene
Title: Sphallerocarpus gracilis polysaccharide protects pancreatic ?-cells via regulation of the bax/bcl-2, caspase-3, pdx-1 and insulin signalling pathways.

Results found that Pdx-1 overexpression exerts its effects on islet cell replication via a noncell-autonomous mechanism involving induction of a secreted soluble factor or factors. Also, Inhbb is identified as one factor mediating the proliferative effect of Pdx-1.
Title: A Pdx-1-Regulated Soluble Factor Activates Rat and Human Islet Cell Proliferation.

restoring proteins in the diet after birth in low protein offspring dramatically impairs glucose homeostasis in early adulthood, by altering Pdx1 expression and downstream-target genes increasing the risk to develop type 2 diabetes.
Title: Regulation of postnatal pancreatic Pdx1 and downstream target genes after gestational exposure to protein restriction in rats.

These results suggest that a secreted luciferase reporter system in vivo enables not only the monitoring of promoter activity but also a quantitative and minimally invasive assessment of physiological and pathological changes in small cell masses.
Title: Quantitative assessment of Pdx1 promoter activity in vivo using a secreted luciferase reporter system.

PASK phosphorylates and inactivates GSK3beta, thereby preventing PDX-1 serine phosphorylation and alleviating GSK3beta-mediated PDX-1 protein degradation in pancreatic beta-cells.
Title: Per-Arnt-Sim kinase regulates pancreatic duodenal homeobox-1 protein stability via phosphorylation of glycogen synthase kinase 3? in pancreatic ?-cells.

The following Pdx1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pdx1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa06363	NM_022852.3 Latest version!	Rattus norvegicus pancreatic and duodenal homeobox 1 (Pdx1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pdx1 ( NM_022852.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_074043.3 Rattus norvegicus pancreatic and duodenal homeobox 1 (Pdx1), mRNA.

Price & Availability↑

CloneID	ORa06363
Clone ID Related Accession (Same CDS sequence)	NM_022852.3
Accession Version	NM_022852.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 852bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Rattus norvegicus(Norway rat)
Product	pancreas/duodenum homeobox protein 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U04833.1. On Jul 30, 2004 this sequence version replaced NM_022852.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U04833.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01906348, SAMN01906349 [ECO:0000348] ##Evidence-Data-END##

ATGAATAGTG AGGAGCAGTA CTACGCGGCC ACACAGCTCT ACAAGGACCC GTGCGCATTC 
CAGAGGGGTC CGGTGCCAGA GTTCAGTGCT AATCCCCCTG CGTGCCTGTA CATGGGCCGC 
CAGCCCCCAC CTCCGCCGAC ACCCCAGTTT GCAGGCTCGC TGGGAACGCT GGAACAGGGA 
AGTCCCCCGG ACATCTCCCC ATACGAAGTG CCCCCGCTCG CCGATGACCC GGCTGGCGCG 
CACCTCCACC ACCACCTCCC AGCTCAGCTC GGGCTCGCCC ATCCACCTCC CGGACCTTTC 
CCGAATGGAA CCGAGACTGG GGGCCTGGAA GAGCCCAGCC GCGTTCATCT CCCTTTCCCG 
TGGATGAAAT CCACCAAAGC TCACGCGTGG AAAAGCCAGT GGGCAGGAGG TGCATACGCA 
GCAGAACCGG AGGAGAATAA GAGGACCCGT ACAGCCTACA CTCGGGCCCA GCTGCTGGAG 
CTGGAGAAGG AATTCTTATT TAACAAATAC ATCTCCCGGC CTCGCCGGGT GGAGCTGGCA 
GTGATGCTCA ACTTGACTGA GAGACACATC AAAATCTGGT TCCAAAACCG TCGCATGAAG 
TGGAAGAAAG AGGAAGATAA GAAACGTAGT AGCGGGACAA CGAGCGGGGG CGGTGGGGGC 
GAAGAGCCGG AGCAGGATTG TGCCGTAACC TCGGGCGAGG AGCTGCTGGC ATTGCCAAAG 
CCACCACCTC CCGGAGGTGT TGTGCCCTCA GGCGTCCCTG CTGCTGCCCG GGAGGGCCGA 
CTGCCTTCCG GCCTTAGTGC GTCCCCACAG CCCTCCAGCA TCGCGCCACT GCGACCGCAG 
GAACCCCGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_074043.3
CDS	101..952
Translation	MNSEEQYYAATQLYKDPCAFQRGPVPEFSANPPACLYMGRQPPPPPTPQFAGSLGTLEQGSPPDISPYEVPPLADDPAGAHLHHHLPAQLGLAHPPPGPFPNGTETGGLEEPSRVHLPFPWMKSTKAHAWKSQWAGGAYAAEPEENKRTRTAYTRAQLLELEKEFLFNKYISRPRRVELAVMLNLTERHIKIWFQNRRMKWKKEEDKKRSSGTTSGGGGGEEPEQDCAVTSGEELLALPKPPPPGGVVPSGVPAAAREGRLPSGLSASPQPSSIAPLRPQEPR

Target ORF information:

RefSeq Version	NM_022852.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus pancreatic and duodenal homeobox 1 (Pdx1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022852.3

ORF Insert Sequence:

ATGAATAGTG AGGAGCAGTA CTACGCGGCC ACACAGCTCT ACAAGGACCC GTGCGCATTC 
CAGAGGGGTC CGGTGCCAGA GTTCAGTGCT AATCCCCCTG CGTGCCTGTA CATGGGCCGC 
CAGCCCCCAC CTCCGCCGAC ACCCCAGTTT GCAGGCTCGC TGGGAACGCT GGAACAGGGA 
AGTCCCCCGG ACATCTCCCC ATACGAAGTG CCCCCGCTCG CCGATGACCC GGCTGGCGCG 
CACCTCCACC ACCACCTCCC AGCTCAGCTC GGGCTCGCCC ATCCACCTCC CGGACCTTTC 
CCGAATGGAA CCGAGACTGG GGGCCTGGAA GAGCCCAGCC GCGTTCATCT CCCTTTCCCG 
TGGATGAAAT CCACCAAAGC TCACGCGTGG AAAAGCCAGT GGGCAGGAGG TGCATACGCA 
GCAGAACCGG AGGAGAATAA GAGGACCCGT ACAGCCTACA CTCGGGCCCA GCTGCTGGAG 
CTGGAGAAGG AATTCTTATT TAACAAATAC ATCTCCCGGC CTCGCCGGGT GGAGCTGGCA 
GTGATGCTCA ACTTGACTGA GAGACACATC AAAATCTGGT TCCAAAACCG TCGCATGAAG 
TGGAAGAAAG AGGAAGATAA GAAACGTAGT AGCGGGACAA CGAGCGGGGG CGGTGGGGGC 
GAAGAGCCGG AGCAGGATTG TGCCGTAACC TCGGGCGAGG AGCTGCTGGC ATTGCCAAAG 
CCACCACCTC CCGGAGGTGT TGTGCCCTCA GGCGTCCCTG CTGCTGCCCG GGAGGGCCGA 
CTGCCTTCCG GCCTTAGTGC GTCCCCACAG CCCTCCAGCA TCGCGCCACT GCGACCGCAG 
GAACCCCGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The Synergy of Two Factors on Insulin Expression.
Cellular reprogramming(2018 Jan)
Luo Y,Xu Y,Wang ZY,Li X,Xing WB,Zhang TC

Sphallerocarpus gracilis polysaccharide protects pancreatic ?-cells via regulation of the bax/bcl-2, caspase-3, pdx-1 and insulin signalling pathways.
International journal of biological macromolecules93(Pt A)829-836(2016 Dec)
Guo J,Wang J,Song S,Liu Q,Huang Y,Xu Y,Wei Y,Zhang J

A Pdx-1-Regulated Soluble Factor Activates Rat and Human Islet Cell Proliferation.
Molecular and cellular biology36(23)2918-2930(2016 Dec)
Hayes HL,Zhang L,Becker TC,Haldeman JM,Stephens SB,Arlotto M,Moss LG,Newgard CB,Hohmeier HE

Regulation of postnatal pancreatic Pdx1 and downstream target genes after gestational exposure to protein restriction in rats.
Reproduction (Cambridge, England)149(3)293-303(2015 Mar)
Abuzgaia AM,Hardy DB,Arany E

Identification and differentiation of PDX1 ?-cell progenitors within the human pancreatic epithelium.
World journal of diabetes5(1)59-68(2014 Feb)
Seeberger KL,Anderson SJ,Ellis CE,Yeung TY,Korbutt GS