Tle5 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tle5
Entrez Gene ID	29466
Full Name	amino-terminal enhancer of split
Synonyms	Grg,Grg-5
General protein information	Preferred Names amino-terminal enhancer of split Names amino-terminal enhancer of split R-esp1 amino enhancer of split groucho-related protein 5 related to Drosophila groucho
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 7 Map Location: 7q11
Summary	may be involved in negative regulation of Enhancer of split proteins [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_019220.1	NP_062093.1	TLE family member 5

Pathways from BioSystems

Homologies

Homo sapiens (human)	AES	NP_945320.1
Bos taurus (cattle)	AES	NP_001121969.1
Mus musculus (house mouse)	Aes	NP_034477.1
Rattus norvegicus (Norway rat)	Aes	NP_062093.1
Gallus gallus (chicken)	AES	XP_003642896.2
	LOC712353	XP_001099930.2
Pan troglodytes (chimpanzee)	AES	XP_001153657.1
Xenopus tropicalis (tropical clawed frog)	aes	NP_001039102.1
Danio rerio (zebrafish)	aes	NP_956717.1
Danio rerio (zebrafish)	chico	NP_570986.1

Gene Ontology
Bibliography

Related articles in PubMed

Pooled-matrix protein interaction screens using Barcode Fusion Genetics.
Yachie N, Petsalaki E, Mellor JC, Weile J, Jacob Y, Verby M, Ozturk SB, Li S, Cote AG, Mosca R, Knapp JJ, Ko M, Yu A, Gebbia M, Sahni N, Yi S, Tyagi T, Sheykhkarimli D, Roth JF, Wong C, Musa L, Snider J, Liu YC, Yu H, Braun P, Stagljar I, Hao T, Calderwood MA, Pelletier L, Aloy P, Hill DE, Vidal M, Roth FP
Molecular systems biology12(4)863(2016 Apr)

TLE1 is an anoikis regulator and is downregulated by Bit1 in breast cancer cells.
Brunquell C, Biliran H, Jennings S, Ireland SK, Chen R, Ruoslahti E
Molecular cancer research : MCR10(11)1482-95(2012 Nov)

Uncovering early response of gene regulatory networks in ESCs by systematic induction of transcription factors.
Nishiyama A, Xin L, Sharov AA, Thomas M, Mowrer G, Meyers E, Piao Y, Mehta S, Yee S, Nakatake Y, Stagg C, Sharova L, Correa-Cerro LS, Bassey U, Hoang H, Kim E, Tapnio R, Qian Y, Dudekula D, Zalzman M, Li M, Falco G, Yang HT, Lee SL, Monti M, Stanghellini I, Islam MN, Nagaraja R, Goldberg I, Wang W, Longo DL, Schlessinger D, Ko MS
Cell stem cell5(4)420-33(2009 Oct)

Groucho binds two conserved regions of LEF-1 for HDAC-dependent repression.
Arce L, Pate KT, Waterman ML
BMC cancer9159(2009 May)

The Groucho-related gene family regulates the gonadotropin-releasing hormone gene through interaction with the homeodomain proteins MSX1 and OCT1.
Rave-Harel N, Miller NL, Givens ML, Mellon PL
The Journal of biological chemistry280(35)30975-83(2005 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Tle5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tle5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa04069	NM_019220.1 Latest version!	Rattus norvegicus TLE family member 5, transcriptional modulator (Tle5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tle5 ( NM_019220.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_062093.1 Rattus norvegicus TLE family member 5, transcriptional modulator (Tle5), mRNA.

Price & Availability↑

CloneID	ORa04069
Clone ID Related Accession (Same CDS sequence)	NM_019220.1
Accession Version	NM_019220.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 594bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-06
Organism	Rattus norvegicus(Norway rat)
Product	TLE family member 5
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from L14462.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: L14462.1, DY312974.1 [ECO:0000332] ##Evidence-Data-END##

ATGATGTTTC CGCAAAGCCG GCACTCGGGC TCCTCCCACC TCCCCCAGCA GCTCAAGTTC 
ACCACCTCCG ACTCCTGCGA CCGCATCAAG GATGAGTTCC AGCTGCTACA AGCGCAGTAC 
CACAGCCTGA AGCTGGAGTG TGACAAGCTG GCCAGCGAGA AGTCAGAGAT GCAAAGGCAT 
TACGTCATGT ACTATGAGAT GTCTTACGGA TTGAACATCG AGATGCACAA ACAGGCTGAA 
ATTGTGAAGA GGCTGAATGG GATTTGCGCC CAGGTTCTAC CCTATTTGTC CCAGGAGCAT 
CAGCAGCAGG TCCTGGGAGC TATCGAGAGA GCCAAGCAGG TCACGGCTCC CGAGCTGAAC 
TCCATCATCC GACAGCAGCT CCAGGCTCAC CAGCTGTCCC AGCTCCAGGC GCTGGCCCTG 
CCCCTGACAC CGCTGCCTGT CGGGCTCCAG CCGCCATCCC TCCCTGCGGT CAGCGCAGGC 
ACAGGCCTGC TGTCACTCTC TGCTCTGGGC TCTCAGACCC ACCTCTCCAA GGAGGACAAG 
AACGGACACG ATGGGGACAC CCACCAGGAG GATGATGGAG AGAAGTCCGA TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_062093.1
CDS	84..677
Translation	MMFPQSRHSGSSHLPQQLKFTTSDSCDRIKDEFQLLQAQYHSLKLECDKLASEKSEMQRHYVMYYEMSYGLNIEMHKQAEIVKRLNGICAQVLPYLSQEHQQQVLGAIERAKQVTAPELNSIIRQQLQAHQLSQLQALALPLTPLPVGLQPPSLPAVSAGTGLLSLSALGSQTHLSKEDKNGHDGDTHQEDDGEKSD

Target ORF information:

RefSeq Version	NM_019220.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus TLE family member 5, transcriptional modulator (Tle5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019220.1

ORF Insert Sequence:

ATGATGTTTC CGCAAAGCCG GCACTCGGGC TCCTCCCACC TCCCCCAGCA GCTCAAGTTC 
ACCACCTCCG ACTCCTGCGA CCGCATCAAG GATGAGTTCC AGCTGCTACA AGCGCAGTAC 
CACAGCCTGA AGCTGGAGTG TGACAAGCTG GCCAGCGAGA AGTCAGAGAT GCAAAGGCAT 
TACGTCATGT ACTATGAGAT GTCTTACGGA TTGAACATCG AGATGCACAA ACAGGCTGAA 
ATTGTGAAGA GGCTGAATGG GATTTGCGCC CAGGTTCTAC CCTATTTGTC CCAGGAGCAT 
CAGCAGCAGG TCCTGGGAGC TATCGAGAGA GCCAAGCAGG TCACGGCTCC CGAGCTGAAC 
TCCATCATCC GACAGCAGCT CCAGGCTCAC CAGCTGTCCC AGCTCCAGGC GCTGGCCCTG 
CCCCTGACAC CGCTGCCTGT CGGGCTCCAG CCGCCATCCC TCCCTGCGGT CAGCGCAGGC 
ACAGGCCTGC TGTCACTCTC TGCTCTGGGC TCTCAGACCC ACCTCTCCAA GGAGGACAAG 
AACGGACACG ATGGGGACAC CCACCAGGAG GATGATGGAG AGAAGTCCGA TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Pooled-matrix protein interaction screens using Barcode Fusion Genetics.
Molecular systems biology12(4)863(2016 Apr)
Yachie N,Petsalaki E,Mellor JC,Weile J,Jacob Y,Verby M,Ozturk SB,Li S,Cote AG,Mosca R,Knapp JJ,Ko M,Yu A,Gebbia M,Sahni N,Yi S,Tyagi T,Sheykhkarimli D,Roth JF,Wong C,Musa L,Snider J,Liu YC,Yu H,Braun P,Stagljar I,Hao T,Calderwood MA,Pelletier L,Aloy P,Hill DE,Vidal M,Roth FP

TLE1 is an anoikis regulator and is downregulated by Bit1 in breast cancer cells.
Molecular cancer research : MCR10(11)1482-95(2012 Nov)
Brunquell C,Biliran H,Jennings S,Ireland SK,Chen R,Ruoslahti E

Uncovering early response of gene regulatory networks in ESCs by systematic induction of transcription factors.
Cell stem cell5(4)420-33(2009 Oct)
Nishiyama A,Xin L,Sharov AA,Thomas M,Mowrer G,Meyers E,Piao Y,Mehta S,Yee S,Nakatake Y,Stagg C,Sharova L,Correa-Cerro LS,Bassey U,Hoang H,Kim E,Tapnio R,Qian Y,Dudekula D,Zalzman M,Li M,Falco G,Yang HT,Lee SL,Monti M,Stanghellini I,Islam MN,Nagaraja R,Goldberg I,Wang W,Longo DL,Schlessinger D,Ko MS

Groucho binds two conserved regions of LEF-1 for HDAC-dependent repression.
BMC cancer9159(2009 May)
Arce L,Pate KT,Waterman ML

The Groucho-related gene family regulates the gonadotropin-releasing hormone gene through interaction with the homeodomain proteins MSX1 and OCT1.
The Journal of biological chemistry280(35)30975-83(2005 Sep)
Rave-Harel N,Miller NL,Givens ML,Mellon PL