Tesk1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tesk1
Entrez Gene ID	29460
Full Name	testis-specific kinase 1
General protein information	Preferred Names testis-specific kinase 1 Names dual specificity testis-specific protein kinase 1 testicular protein kinase 1 testis specific protein kinase 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 5 Map Location: 5q22
Summary	serine/threonine protein kinase; phosphorylates myelin basic protein (MBP) and histones in vitro; may play a central role in spermatogenesis [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_031578.1	NP_113766.1	dual specificity testis-specific protein kinase 1

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	si:ch211-113e8.3	XP_683848.1
Danio rerio (zebrafish)	si:dkeyp-75h12.2	XP_687879.2
Homo sapiens (human)	TESK1	NP_006276.2
Canis lupus familiaris (dog)	TESK1	XP_005627046.1
Rattus norvegicus (Norway rat)	Tesk1	NP_113766.1
	TESK1	XP_001089847.1
Mus musculus (house mouse)	Tesk1	NP_035701.3
Pan troglodytes (chimpanzee)	TESK1	XP_520558.3
Bos taurus (cattle)	TESK1	NP_001165698.1
Gallus gallus (chicken)	TESK1	XP_427433.4

Gene Ontology
Bibliography

Related articles in PubMed

Spatiotemporal expression of testicular protein kinase 1 after rat sciatic nerve injury.
Lou D, Sun B, Wei H, Deng X, Chen H, Xu D, Li G, Xu H, Wang Y
Journal of molecular neuroscience : MN47(1)180-91(2012 May)

Spred1 and TESK1--two new interaction partners of the kinase MARKK/TAO1 that link the microtubule and actin cytoskeleton.
Johne C, Matenia D, Li XY, Timm T, Balusamy K, Mandelkow EM
Molecular biology of the cell19(4)1391-403(2008 Apr)

Tesk1 interacts with Spry2 to abrogate its inhibition of ERK phosphorylation downstream of receptor tyrosine kinase signaling.
Chandramouli S, Yu CY, Yusoff P, Lao DH, Leong HF, Mizuno K, Guy GR
The Journal of biological chemistry283(3)1679-91(2008 Jan)

Actopaxin interacts with TESK1 to regulate cell spreading on fibronectin.
LaLonde DP, Brown MC, Bouverat BP, Turner CE
The Journal of biological chemistry280(22)21680-8(2005 Jun)

Dual specificity protein kinase activity of testis-specific protein kinase 1 and its regulation by autophosphorylation of serine-215 within the activation loop.
Toshima J, Tanaka T, Mizuno K
The Journal of biological chemistry274(17)12171-6(1999 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

TESK1 plays important roles in promyelinating Schwann cells, potentially through subcellular localization change and participation in integrin-mediated actin reorganization.
Title: Spatiotemporal expression of testicular protein kinase 1 after rat sciatic nerve injury.

By selectively modulating the downstream effects of Spry2, Tesk1 may thus serve as a molecular determinant of the signaling outcome.
Title: Tesk1 interacts with Spry2 to abrogate its inhibition of ERK phosphorylation downstream of receptor tyrosine kinase signaling.

The following Tesk1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tesk1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa08152	NM_031578.1 Latest version!	Rattus norvegicus testis associated actin remodelling kinase 1 (Tesk1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tesk1 ( NM_031578.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_113766.1 Rattus norvegicus testis associated actin remodelling kinase 1 (Tesk1), mRNA.

Price & Availability↑

CloneID	ORa08152
Clone ID Related Accession (Same CDS sequence)	NM_031578.1
Accession Version	NM_031578.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1887bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Rattus norvegicus(Norway rat)
Product	dual specificity testis-specific protein kinase 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from D50864.1. On Dec 17, 2003 this sequence version replaced XM_346736.1. ##Evidence-Data-START## Transcript exon combination :: D50864.1, BC081773.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGCCGGGG AACGGCCACC GCTGCGGGGC CCCGGGCCCG GAGAGACTCC GGTGGAGGGG 
CCGGGGGGCG CAGGCGGAGG CCCGGGCCGG GGCCGGCCCT CCTCCTACCG GGCCCTCCGC 
AGCGCCGTGT CCAGCCTGGC GCGCGTGGAC GATTTCGACT GCGCAGAGAA GATCGGGGCT 
GGTTTCTTCT CTGAGGTCTA TAAGGTTCGG CACCGACAGT CGGGACAAGT CATGGTGCTG 
AAGATGAACA AGCTCCCCAG TAACCGGAGC AACACTCTAA GGGAGGTGCA GCTGATGAAC 
CGGCTCCGGC ATCCTAACAT CCTCAGGTTC ATGGGGGTCT GTGTGCACCA AGGGCAGCTG 
CACGCGCTTA CAGAGTATAT GAATGGGGGT ACCCTGGAAC AGCTGCTGAG CTCCCCAGAA 
CCCCTCTCCT GGCCAGTCAG GCTCCACCTA GCACTGGACA TTGCACAAGG CCTACGGTAC 
CTACACGCCA AAGGTGTGTT TCACCGAGAC CTCACATCCA AGAACTGTCT GGTCCGACGG 
GAAGACGGAG GCTTCACAGC TGTTGTGGGT GACTTCGGAC TGGCTGAAAA GATCCCTGTG 
TATAGGGAAG GGGCAAGGAA GGAGCCATTG GCTGTGGTGG GCTCCCCATA CTGGATGGCC 
CCAGAGGTGT TGCGGGGAGA GCTGTATGAT GAGAAGGCCG ACGTCTTCGC CTTTGGGATT 
GTCCTCTGTG AGCTCATCGC CCGAGTACCT GCAGACCCTG ACTACCTACC CCGTACTGAG 
GACTTTGGTC TGGATGTGCC TGCTTTTCGA ACTCTGGTGG GAAATGACTG TCCGCTACCT 
TTCCTGCTTC TGGCCATCCA CTGCTGCAGC ATGGAACCCA GCGCCCGTGC CCCTTTTACT 
GAAATTACCC AGCACCTGGA ACAAATCCTG GAGCAGCTGC CTGAGCCCAC GCCCCTCGCC 
AAGATGCCCC TTGCCAAGGC TCCCTTGACA TACAATCAGG GGTCTGTTCC AAGAGGAGGT 
CCCTCTGCCA CGCTTCCCAG GTCAGACCCC CGGCTCTCCC GAAGCCGGTC AGACCTCTTC 
CTGCCACCTT CACCAGAATC ACCCCCCAGT TGGGGGGACA ATCTAACACG AGTCAACCCC 
TTCTCACTGC GGGAAGACCT CAGGGGTGGC AAGATCAAAC TTCTGGACAC ACCCTGCAAG 
CCGGCCACTC CACTGCCCCT GGTGCCACCA TCACCACTGA CCTCTACCCA GCTGCCCTTG 
GTGGCCTCTC CAGAGTCCCT GGTCCAGCCT GAGACTCCCG TCCGCCGCTG TCGTTCACTC 
CCTTCATCCC CTGAGCTCCC CCGACGGATG GAAACTGCAC TGCCAGGTCC TGGCCCGTCC 
CCCGTGGGCC CCTCGACTGA GGAAAGGATG GACTGTGAGG GCAGCAGCCC TGAGCCAGAG 
CCCCCAGGGC CGGCTCCCCA GCTGCCTCTG GCTGTGGCCA CTGACAACTT CATCAGTACG 
TGTTCCTCAG CCTCTCAGCC CTGGTCTGCT AGACCAGGAC CTTCCCTCAA TAATAACCCC 
CCTGCTGTGG TGGTGAATTC CCCACAAGGA TGGGCGAGGG AGCCCTGGAA CCGGGCACAG 
CATAGCCTTC CCCGGGCAGC AGCCCTGGAG CGGACAGAAC CCTCACCACC CCCATCAGCT 
CCCCGGGAGC AGGAGGAGGG GCTGCCCTGC CCTGGCTGCT GCCTCAGCCC CTTCAGCTTT 
GGCTTCCTAT CCATGTGCCC CCGCCCTACC CCAGCTGTTG CCCGCTACCG CAACTTGAAC 
TGTGAGGCGG GCAGTCTTCT CTGCCACCGA GGGCATCATG CCAAGCCACC CACACCCAGC 
CTGCAGCTGC CTGGGGCACG CTCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_113766.1
CDS	1131..3017
Translation	MAGERPPLRGPGPGETPVEGPGGAGGGPGRGRPSSYRALRSAVSSLARVDDFDCAEKIGAGFFSEVYKVRHRQSGQVMVLKMNKLPSNRSNTLREVQLMNRLRHPNILRFMGVCVHQGQLHALTEYMNGGTLEQLLSSPEPLSWPVRLHLALDIAQGLRYLHAKGVFHRDLTSKNCLVRREDGGFTAVVGDFGLAEKIPVYREGARKEPLAVVGSPYWMAPEVLRGELYDEKADVFAFGIVLCELIARVPADPDYLPRTEDFGLDVPAFRTLVGNDCPLPFLLLAIHCCSMEPSARAPFTEITQHLEQILEQLPEPTPLAKMPLAKAPLTYNQGSVPRGGPSATLPRSDPRLSRSRSDLFLPPSPESPPSWGDNLTRVNPFSLREDLRGGKIKLLDTPCKPATPLPLVPPSPLTSTQLPLVASPESLVQPETPVRRCRSLPSSPELPRRMETALPGPGPSPVGPSTEERMDCEGSSPEPEPPGPAPQLPLAVATDNFISTCSSASQPWSARPGPSLNNNPPAVVVNSPQGWAREPWNRAQHSLPRAAALERTEPSPPPSAPREQEEGLPCPGCCLSPFSFGFLSMCPRPTPAVARYRNLNCEAGSLLCHRGHHAKPPTPSLQLPGARS

Target ORF information:

RefSeq Version	NM_031578.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus testis associated actin remodelling kinase 1 (Tesk1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031578.1

ORF Insert Sequence:

ATGGCCGGGG AACGGCCACC GCTGCGGGGC CCCGGGCCCG GAGAGACTCC GGTGGAGGGG 
CCGGGGGGCG CAGGCGGAGG CCCGGGCCGG GGCCGGCCCT CCTCCTACCG GGCCCTCCGC 
AGCGCCGTGT CCAGCCTGGC GCGCGTGGAC GATTTCGACT GCGCAGAGAA GATCGGGGCT 
GGTTTCTTCT CTGAGGTCTA TAAGGTTCGG CACCGACAGT CGGGACAAGT CATGGTGCTG 
AAGATGAACA AGCTCCCCAG TAACCGGAGC AACACTCTAA GGGAGGTGCA GCTGATGAAC 
CGGCTCCGGC ATCCTAACAT CCTCAGGTTC ATGGGGGTCT GTGTGCACCA AGGGCAGCTG 
CACGCGCTTA CAGAGTATAT GAATGGGGGT ACCCTGGAAC AGCTGCTGAG CTCCCCAGAA 
CCCCTCTCCT GGCCAGTCAG GCTCCACCTA GCACTGGACA TTGCACAAGG CCTACGGTAC 
CTACACGCCA AAGGTGTGTT TCACCGAGAC CTCACATCCA AGAACTGTCT GGTCCGACGG 
GAAGACGGAG GCTTCACAGC TGTTGTGGGT GACTTCGGAC TGGCTGAAAA GATCCCTGTG 
TATAGGGAAG GGGCAAGGAA GGAGCCATTG GCTGTGGTGG GCTCCCCATA CTGGATGGCC 
CCAGAGGTGT TGCGGGGAGA GCTGTATGAT GAGAAGGCCG ACGTCTTCGC CTTTGGGATT 
GTCCTCTGTG AGCTCATCGC CCGAGTACCT GCAGACCCTG ACTACCTACC CCGTACTGAG 
GACTTTGGTC TGGATGTGCC TGCTTTTCGA ACTCTGGTGG GAAATGACTG TCCGCTACCT 
TTCCTGCTTC TGGCCATCCA CTGCTGCAGC ATGGAACCCA GCGCCCGTGC CCCTTTTACT 
GAAATTACCC AGCACCTGGA ACAAATCCTG GAGCAGCTGC CTGAGCCCAC GCCCCTCGCC 
AAGATGCCCC TTGCCAAGGC TCCCTTGACA TACAATCAGG GGTCTGTTCC AAGAGGAGGT 
CCCTCTGCCA CGCTTCCCAG GTCAGACCCC CGGCTCTCCC GAAGCCGGTC AGACCTCTTC 
CTGCCACCTT CACCAGAATC ACCCCCCAGT TGGGGGGACA ATCTAACACG AGTCAACCCC 
TTCTCACTGC GGGAAGACCT CAGGGGTGGC AAGATCAAAC TTCTGGACAC ACCCTGCAAG 
CCGGCCACTC CACTGCCCCT GGTGCCACCA TCACCACTGA CCTCTACCCA GCTGCCCTTG 
GTGGCCTCTC CAGAGTCCCT GGTCCAGCCT GAGACTCCCG TCCGCCGCTG TCGTTCACTC 
CCTTCATCCC CTGAGCTCCC CCGACGGATG GAAACTGCAC TGCCAGGTCC TGGCCCGTCC 
CCCGTGGGCC CCTCGACTGA GGAAAGGATG GACTGTGAGG GCAGCAGCCC TGAGCCAGAG 
CCCCCAGGGC CGGCTCCCCA GCTGCCTCTG GCTGTGGCCA CTGACAACTT CATCAGTACG 
TGTTCCTCAG CCTCTCAGCC CTGGTCTGCT AGACCAGGAC CTTCCCTCAA TAATAACCCC 
CCTGCTGTGG TGGTGAATTC CCCACAAGGA TGGGCGAGGG AGCCCTGGAA CCGGGCACAG 
CATAGCCTTC CCCGGGCAGC AGCCCTGGAG CGGACAGAAC CCTCACCACC CCCATCAGCT 
CCCCGGGAGC AGGAGGAGGG GCTGCCCTGC CCTGGCTGCT GCCTCAGCCC CTTCAGCTTT 
GGCTTCCTAT CCATGTGCCC CCGCCCTACC CCAGCTGTTG CCCGCTACCG CAACTTGAAC 
TGTGAGGCGG GCAGTCTTCT CTGCCACCGA GGGCATCATG CCAAGCCACC CACACCCAGC 
CTGCAGCTGC CTGGGGCACG CTCTTAG