Msra cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Msra
Entrez Gene ID	29447
Full Name	methionine sulfoxide reductase A
General protein information	Preferred Names methionine sulfoxide reductase A Names mitochondrial peptide methionine sulfoxide reductase PMSR peptide Met(O) reductase peptide methionine sulfoxide reductase peptide-methionine (S)-S-oxide reductase protein-methionine-S-oxide reductase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 15 Map Location: 15p12
Summary	catalyzes the reduction of methionine sulphoxide to methionine in proteins [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_053307.2	NP_445759.1	mitochondrial peptide methionine sulfoxide reductase
XM_008770745.2	XP_008768967.2	mitochondrial peptide methionine sulfoxide reductase isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	LOC608103	XP_850101.2
Mus musculus (house mouse)	Msra	NP_080598.2
	MSRA	XP_001089893.1
Rattus norvegicus (Norway rat)	Msra	NP_445759.1
Eremothecium gossypii	AGOS_AFR239W	NP_985786.2
Danio rerio (zebrafish)	si:dkey-221h15.1	NP_001076362.1
Xenopus tropicalis (tropical clawed frog)	msra.1	XP_002933632.2
Pan troglodytes (chimpanzee)	MSRA	XP_001138036.1
Homo sapiens (human)	MSRA	NP_036463.1
Bos taurus (cattle)	MSRA	NP_776539.1
Gallus gallus (chicken)	MSRA	XP_004935948.1
Oryza sativa (rice)	Os04g0482000	NP_001053116.1

Gene Ontology
Bibliography

Related articles in PubMed

Methionine sulfoxide reductase A negatively controls microglia-mediated neuroinflammation via inhibiting ROS/MAPKs/NF-?B signaling pathways through a catalytic antioxidant function.
Fan H, Wu PF, Zhang L, Hu ZL, Wang W, Guan XL, Luo H, Ni M, Yang JW, Li MX, Chen JG, Wang F
Antioxidants & redox signaling22(10)832-47(2015 Apr)

Independent roles of methionine sulfoxide reductase A in mitochondrial ATP synthesis and as antioxidant in retinal pigment epithelial cells.
Dun Y, Vargas J, Brot N, Finnemann SC
Free radical biology & medicine651340-51(2013 Dec)

A specific and rapid colorimetric method to monitor the activity of methionine sulfoxide reductase A.
Wu PF, Zhang Z, Guan XL, Li YL, Zeng JH, Zhang JJ, Long LH, Hu ZL, Wang F, Chen JG
Enzyme and microbial technology53(6-7)391-7(2013 Dec)

Methionine sulfoxide reductase A attenuates heme oxygenase-1 induction through inhibition of Nrf2 activation.
Kim JY, Choi SH, Lee E, Kang YJ, Kim HY
Archives of biochemistry and biophysics528(2)134-40(2012 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The study provides the first direct evidence for the biological significance of MsrA in microglia-mediated neuroinflammation.
Title: Methionine sulfoxide reductase A negatively controls microglia-mediated neuroinflammation via inhibiting ROS/MAPKs/NF-?B signaling pathways through a catalytic antioxidant function.

MsrA probably enhances ATP synthesis by increasing complex IV activity.
Title: Independent roles of methionine sulfoxide reductase A in mitochondrial ATP synthesis and as antioxidant in retinal pigment epithelial cells.

Specific colorimetric microplate assay for testing MsrA activity quickly, based on the fact that MsrA can catalyze the reduction of methyl sulfoxides and simultaneously oxidize dithiothreitol, whose color can be produced by reacting with Ellman's reagent.
Title: A specific and rapid colorimetric method to monitor the activity of methionine sulfoxide reductase A.

MsrA overexpression in MsrA-depleted cells led to the reduction of increased HO-1 expression, and suppressed nuclear accumulation of Nrf2.
Title: Methionine sulfoxide reductase A attenuates heme oxygenase-1 induction through inhibition of Nrf2 activation.

The data indicate that Msr activity is reduced by obesity and may alter oxidative stress signalling of obesity.
Title: Obesity reduces methionine sulphoxide reductase activity in visceral adipose tissue.

The following Msra gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Msra cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa42434	NM_053307.2 Latest version!	Rattus norvegicus methionine sulfoxide reductase A (Msra), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
ORa42434	XM_008770745.2 Latest version!	Rattus norvegicus methionine sulfoxide reductase A (Msra), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Msra ( NM_053307.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_445759.1 Rattus norvegicus methionine sulfoxide reductase A (Msra), mRNA.

Price & Availability↑

CloneID	ORa42434
Clone ID Related Accession (Same CDS sequence)	NM_053307.2 , XM_008770745.2
Accession Version	NM_053307.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 702bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	mitochondrial peptide methionine sulfoxide reductase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC087009.1. On or before Apr 19, 2005 this sequence version replaced XM_573806.1, NM_053307.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC087009.1 [ECO:0000332] ##Evidence-Data-END##

ATGCTCTCCG CCTCCAGAAG GACTCTCCAG CTCCTCTCCA GCTCCATCCC GGTACGGATG 
ATGGGCGACT CATCTTCGAA GGTCATCAGC GCGGAGGAAG CTTTGCCAGG ACGCACAGAG 
TCGATCCCCG TAGCAGCCAA ACACCATGTC AGTGGCAACA GAACGGTTGA ACCTTTCCCA 
GAGGGAACAC AGATGGCTGT ATTTGGAATG GGCTGCTTCT GGGGAGCTGA GCGCAAGTTC 
TGGCTCTTGA AAGGAGTGTA TTCAACCCAA GTGGGTTTCG CAGGAGGCTA CACACGCAAT 
CCCACCTACA AAGAGGTCTG CTCAGAAAAA ACCGGTCACG CAGAAGTCGT CCGGGTTGTG 
TACCGGCCAG AGCACGTCAG CTTTGAGGAG CTGCTCAAGG TCTTCTGGGA GAATCACGAC 
CCGACCCAAG GGATGCGCCA AGGCAATGAC TGTGGCACGC AGTATCGATC GGCGGTCTAT 
CCCACCTCCG CCGTGCAGAT GGAAGCTGCC CTGAAATCCA AGGAGGAGTA CCAGAAGGTT 
CTTTCAAAGC ATGGCTTTGG CCCCATCACC ACCGACATCC GAGAGGGACA AGTGTTCTAC 
TACGCCGAAG ATTACCACCA GCAATACCTG AGCAAGAACC CAGACGGCTA CTGTGGCCTT 
GGGGGCACTG GGGTTTCCTG TCCCACGGCC ATTAAAAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_445759.1
CDS	11..712
Translation	MLSASRRTLQLLSSSIPVRMMGDSSSKVISAEEALPGRTESIPVAAKHHVSGNRTVEPFPEGTQMAVFGMGCFWGAERKFWLLKGVYSTQVGFAGGYTRNPTYKEVCSEKTGHAEVVRVVYRPEHVSFEELLKVFWENHDPTQGMRQGNDCGTQYRSAVYPTSAVQMEAALKSKEEYQKVLSKHGFGPITTDIREGQVFYYAEDYHQQYLSKNPDGYCGLGGTGVSCPTAIKK

Target ORF information:

RefSeq Version	NM_053307.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus methionine sulfoxide reductase A (Msra), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053307.2

ORF Insert Sequence:

ATGCTCTCCG CCTCCAGAAG GACTCTCCAG CTCCTCTCCA GCTCCATCCC GGTACGGATG 
ATGGGCGACT CATCTTCGAA GGTCATCAGC GCGGAGGAAG CTTTGCCAGG ACGCACAGAG 
TCGATCCCCG TAGCAGCCAA ACACCATGTC AGTGGCAACA GAACGGTTGA ACCTTTCCCA 
GAGGGAACAC AGATGGCTGT ATTTGGAATG GGCTGCTTCT GGGGAGCTGA GCGCAAGTTC 
TGGCTCTTGA AAGGAGTGTA TTCAACCCAA GTGGGTTTCG CAGGAGGCTA CACACGCAAT 
CCCACCTACA AAGAGGTCTG CTCAGAAAAA ACCGGTCACG CAGAAGTCGT CCGGGTTGTG 
TACCGGCCAG AGCACGTCAG CTTTGAGGAG CTGCTCAAGG TCTTCTGGGA GAATCACGAC 
CCGACCCAAG GGATGCGCCA AGGCAATGAC TGTGGCACGC AGTATCGATC GGCGGTCTAT 
CCCACCTCCG CCGTGCAGAT GGAAGCTGCC CTGAAATCCA AGGAGGAGTA CCAGAAGGTT 
CTTTCAAAGC ATGGCTTTGG CCCCATCACC ACCGACATCC GAGAGGGACA AGTGTTCTAC 
TACGCCGAAG ATTACCACCA GCAATACCTG AGCAAGAACC CAGACGGCTA CTGTGGCCTT 
GGGGGCACTG GGGTTTCCTG TCCCACGGCC ATTAAAAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Msra ( XM_008770745.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008768967.2 Rattus norvegicus methionine sulfoxide reductase A (Msra), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa42434
Clone ID Related Accession (Same CDS sequence)	NM_053307.2 , XM_008770745.2
Accession Version	XM_008770745.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 702bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	mitochondrial peptide methionine sulfoxide reductase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005114.4) and transcript sequence (BC087009.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008770745.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 631 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGCTCTCCG CCTCCAGAAG GACTCTCCAG CTCCTCTCCA GCTCCATCCC GGTACGGATG 
ATGGGCGACT CATCTTCGAA GGTCATCAGC GCGGAGGAAG CTTTGCCAGG ACGCACAGAG 
TCGATCCCCG TAGCAGCCAA ACACCATGTC AGTGGCAACA GAACGGTTGA ACCTTTCCCA 
GAGGGAACAC AGATGGCTGT ATTTGGAATG GGCTGCTTCT GGGGAGCTGA GCGCAAGTTC 
TGGCTCTTGA AAGGAGTGTA TTCAACCCAA GTGGGTTTCG CAGGAGGCTA CACACGCAAT 
CCCACCTACA AAGAGGTCTG CTCAGAAAAA ACCGGTCACG CAGAAGTCGT CCGGGTTGTG 
TACCGGCCAG AGCACGTCAG CTTTGAGGAG CTGCTCAAGG TCTTCTGGGA GAATCACGAC 
CCGACCCAAG GGATGCGCCA AGGCAATGAC TGTGGCACGC AGTATCGATC GGCGGTCTAT 
CCCACCTCCG CCGTGCAGAT GGAAGCTGCC CTGAAATCCA AGGAGGAGTA CCAGAAGGTT 
CTTTCAAAGC ATGGCTTTGG CCCCATCACC ACCGACATCC GAGAGGGACA AGTGTTCTAC 
TACGCCGAAG ATTACCACCA GCAATACCTG AGCAAGAACC CAGACGGCTA CTGTGGCCTT 
GGGGGCACTG GGGTTTCCTG TCCCACGGCC ATTAAAAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008768967.2
CDS	639..1340
Translation	MLSASRRTLQLLSSSIPVRMMGDSSSKVISAEEALPGRTESIPVAAKHHVSGNRTVEPFPEGTQMAVFGMGCFWGAERKFWLLKGVYSTQVGFAGGYTRNPTYKEVCSEKTGHAEVVRVVYRPEHVSFEELLKVFWENHDPTQGMRQGNDCGTQYRSAVYPTSAVQMEAALKSKEEYQKVLSKHGFGPITTDIREGQVFYYAEDYHQQYLSKNPDGYCGLGGTGVSCPTAIKK

Target ORF information:

RefSeq Version	XM_008770745.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus methionine sulfoxide reductase A (Msra), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008770745.2

ORF Insert Sequence:

ATGCTCTCCG CCTCCAGAAG GACTCTCCAG CTCCTCTCCA GCTCCATCCC GGTACGGATG 
ATGGGCGACT CATCTTCGAA GGTCATCAGC GCGGAGGAAG CTTTGCCAGG ACGCACAGAG 
TCGATCCCCG TAGCAGCCAA ACACCATGTC AGTGGCAACA GAACGGTTGA ACCTTTCCCA 
GAGGGAACAC AGATGGCTGT ATTTGGAATG GGCTGCTTCT GGGGAGCTGA GCGCAAGTTC 
TGGCTCTTGA AAGGAGTGTA TTCAACCCAA GTGGGTTTCG CAGGAGGCTA CACACGCAAT 
CCCACCTACA AAGAGGTCTG CTCAGAAAAA ACCGGTCACG CAGAAGTCGT CCGGGTTGTG 
TACCGGCCAG AGCACGTCAG CTTTGAGGAG CTGCTCAAGG TCTTCTGGGA GAATCACGAC 
CCGACCCAAG GGATGCGCCA AGGCAATGAC TGTGGCACGC AGTATCGATC GGCGGTCTAT 
CCCACCTCCG CCGTGCAGAT GGAAGCTGCC CTGAAATCCA AGGAGGAGTA CCAGAAGGTT 
CTTTCAAAGC ATGGCTTTGG CCCCATCACC ACCGACATCC GAGAGGGACA AGTGTTCTAC 
TACGCCGAAG ATTACCACCA GCAATACCTG AGCAAGAACC CAGACGGCTA CTGTGGCCTT 
GGGGGCACTG GGGTTTCCTG TCCCACGGCC ATTAAAAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Methionine sulfoxide reductase A negatively controls microglia-mediated neuroinflammation via inhibiting ROS/MAPKs/NF-?B signaling pathways through a catalytic antioxidant function.
Antioxidants & redox signaling22(10)832-47(2015 Apr)
Fan H,Wu PF,Zhang L,Hu ZL,Wang W,Guan XL,Luo H,Ni M,Yang JW,Li MX,Chen JG,Wang F

Independent roles of methionine sulfoxide reductase A in mitochondrial ATP synthesis and as antioxidant in retinal pigment epithelial cells.
Free radical biology & medicine651340-51(2013 Dec)
Dun Y,Vargas J,Brot N,Finnemann SC

A specific and rapid colorimetric method to monitor the activity of methionine sulfoxide reductase A.
Enzyme and microbial technology53(6-7)391-7(2013 Dec)
Wu PF,Zhang Z,Guan XL,Li YL,Zeng JH,Zhang JJ,Long LH,Hu ZL,Wang F,Chen JG

Methionine sulfoxide reductase A attenuates heme oxygenase-1 induction through inhibition of Nrf2 activation.
Archives of biochemistry and biophysics528(2)134-40(2012 Dec)
Kim JY,Choi SH,Lee E,Kang YJ,Kim HY