Phlda1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Phlda1
Entrez Gene ID	29380
Full Name	pleckstrin homology-like domain, family A, member 1
Synonyms	Tdag
General protein information	Preferred Names pleckstrin homology-like domain, family A, member 1 Names pleckstrin homology-like domain family A member 1 PQ-rich protein PQR protein T-cell death associated proline- and glutamine-rich protein
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 7 Map Location: 7q22
Summary	may mediate induction of apoptosis during neuronal development; induced by growth factors and differentiating agents [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_017180.2	NP_058876.2	pleckstrin homology-like domain family A member 1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	PHLDA1	XP_005629218.1
Mus musculus (house mouse)	Phlda1	NP_033370.2
Danio rerio (zebrafish)	phlda1	NP_001006011.1
Homo sapiens (human)	PHLDA1	NP_031376.3
Pan troglodytes (chimpanzee)	PHLDA1	XP_001161528.1
	LOC719036	XP_001117606.2
Rattus norvegicus (Norway rat)	Phlda1	NP_058876.2
Xenopus tropicalis (tropical clawed frog)	phlda1	NP_001015853.1

Gene Ontology
Bibliography

Related articles in PubMed

Expression of Hsf1, Hsf2, and Phlda1 in cells undergoing cryptorchid-induced apoptosis in rat testes.
Liu F, Xu ZL, Qian XJ, Qiu WY, Huang H
Molecular reproduction and development78(4)283-91(2011 Apr)

A proline- and glutamine-rich protein promotes apoptosis in neuronal cells.
Gomes I, Xiong W, Miki T, Rosner MR
Journal of neurochemistry73(2)612-22(1999 Aug)

A novel gene product that couples TCR signaling to Fas(CD95) expression in activation-induced cell death.
Park CG, Lee SY, Kandala G, Lee SY, Choi Y
Immunity4(6)583-91(1996 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The expression of Phlda1 mRNA is significantly elevated 3 days after induction of cryptorchidism.
Title: Expression of Hsf1, Hsf2, and Phlda1 in cells undergoing cryptorchid-induced apoptosis in rat testes.

The following Phlda1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Phlda1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa03143	NM_017180.2 Latest version!	Rattus norvegicus pleckstrin homology-like domain, family A, member 1 (Phlda1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Phlda1 ( NM_017180.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_058876.2 Rattus norvegicus pleckstrin homology-like domain, family A, member 1 (Phlda1), mRNA.

Price & Availability↑

CloneID	ORa03143
Clone ID Related Accession (Same CDS sequence)	NM_017180.2
Accession Version	NM_017180.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1221bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-21
Organism	Rattus norvegicus(Norway rat)
Product	pleckstrin homology-like domain family A member 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC113741.6. On Sep 1, 2009 this sequence version replaced NM_017180.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: EV764287.1, AF192802.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGAGGCGTA CGCCGGCTGC CGAGCGCCTC TCGGAGCTGG GTTTTCCCCC GCGGCGCGGG 
AGCCAGGAGC CGCCTTTTCC GCTGGGTGTC ACTCGGGGGT GGGGAGGGTG GCCCATTGAA 
AAGCGCTGCG AGGGGCCCCG GCCAGTGCCC TTCAGTGAGC GCTCCGCGGA AGACGGCAGA 
GAGCAGCCCG CTCACGGCTC CGGGATCTTG TGGCGGGTCA GGACTCGCCT GTCCCTCTGC 
CGGGACCCCG AGCCACCGCC GCCACCGCCA CCGCTCTGTC TCCTGCGAGT CAGCCTTCTC 
TGTGCGCTCC GGGCAGGCGG CCGCGGAAGC CGCTGGAGCG AGGACAGCGC ACGGCTGCTA 
CTGCTACCCC CAGCCGGAGC GTCTGGAAGC CTAAAGGCCG AGCGGAGCAG CAGCACCCCT 
TATGCCGGAA GGATGCTGGA GAGCAGCGGT TGCAAGGCGC TGAAGGAAGG AGTGCTGGAA 
AAGCGAAGCG ACGGGCTGCT GCAGCTCTGG AAGAAAAAGT GCTGCATCCT CACTGAGGAA 
GGGCTACTGC TCATACCGCC CAAGCAGGTA CAACATCAGC AGCAGCAACA GCAGCAGCAA 
CAGCCCGGGC AGGGGACAGC CGAACCGTCC CAACCTAGTG GCCCCGCCGT CACCAGCCTT 
GAGCCACCAG CCAAGCTGAA GGAATTGCAC TTTTCCAATA TGAAGACTGT AGACTGCGTG 
GAGCGCAAGG GCAAGTATAT GTACTTCACT GTGGTGATGG CGGAGGGCAA AGAGATCGAC 
TTTCGGTGTC CCCAGGACCA GGGCTGGAAC GCAGAGATCA CGTTGCAGAT GGTGCAGTAC 
AAAAATCGTC AGGCCATACT GGCGGTCAAG TCTACGAGGC AGAAGCAGCA GCACCTGGTC 
CAGCAGCAGC CTCCGCAGAC GCAGCAGATC CAACCCCAGC CCCAGCCGCA GATCCAACCC 
CAGCCCCAGC CGCAGATCCA GCCTCAACCC CAACCCCAAC CCCAACCTCA GCCCCAACCC 
CAGCAGCAGC CCCAGCCCCA ACAGCTCCAC TCCTACCCGC ACCCGCATCC TCATCTTTAC 
CCTCACCCTC ACCCTCACGC GCACTCGCAC CCGCACCCGC ACCCTCACCC TCATCCACAC 
CAACTCCAGC ATGCGCACCA GCCTCTCCAC TCGCAGCCAC AGGGCCACCG GCTTCTCCGC 
AGCACCTCCA ACTCTGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_058876.2
CDS	2..1222
Translation	MRRTPAAERLSELGFPPRRGSQEPPFPLGVTRGWGGWPIEKRCEGPRPVPFSERSAEDGREQPAHGSGILWRVRTRLSLCRDPEPPPPPPPLCLLRVSLLCALRAGGRGSRWSEDSARLLLLPPAGASGSLKAERSSSTPYAGRMLESSGCKALKEGVLEKRSDGLLQLWKKKCCILTEEGLLLIPPKQVQHQQQQQQQQQPGQGTAEPSQPSGPAVTSLEPPAKLKELHFSNMKTVDCVERKGKYMYFTVVMAEGKEIDFRCPQDQGWNAEITLQMVQYKNRQAILAVKSTRQKQQHLVQQQPPQTQQIQPQPQPQIQPQPQPQIQPQPQPQPQPQPQPQQQPQPQQLHSYPHPHPHLYPHPHPHAHSHPHPHPHPHPHQLQHAHQPLHSQPQGHRLLRSTSNSA

Target ORF information:

RefSeq Version	NM_017180.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus pleckstrin homology-like domain, family A, member 1 (Phlda1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017180.2

ORF Insert Sequence:

ATGAGGCGTA CGCCGGCTGC CGAGCGCCTC TCGGAGCTGG GTTTTCCCCC GCGGCGCGGG 
AGCCAGGAGC CGCCTTTTCC GCTGGGTGTC ACTCGGGGGT GGGGAGGGTG GCCCATTGAA 
AAGCGCTGCG AGGGGCCCCG GCCAGTGCCC TTCAGTGAGC GCTCCGCGGA AGACGGCAGA 
GAGCAGCCCG CTCACGGCTC CGGGATCTTG TGGCGGGTCA GGACTCGCCT GTCCCTCTGC 
CGGGACCCCG AGCCACCGCC GCCACCGCCA CCGCTCTGTC TCCTGCGAGT CAGCCTTCTC 
TGTGCGCTCC GGGCAGGCGG CCGCGGAAGC CGCTGGAGCG AGGACAGCGC ACGGCTGCTA 
CTGCTACCCC CAGCCGGAGC GTCTGGAAGC CTAAAGGCCG AGCGGAGCAG CAGCACCCCT 
TATGCCGGAA GGATGCTGGA GAGCAGCGGT TGCAAGGCGC TGAAGGAAGG AGTGCTGGAA 
AAGCGAAGCG ACGGGCTGCT GCAGCTCTGG AAGAAAAAGT GCTGCATCCT CACTGAGGAA 
GGGCTACTGC TCATACCGCC CAAGCAGGTA CAACATCAGC AGCAGCAACA GCAGCAGCAA 
CAGCCCGGGC AGGGGACAGC CGAACCGTCC CAACCTAGTG GCCCCGCCGT CACCAGCCTT 
GAGCCACCAG CCAAGCTGAA GGAATTGCAC TTTTCCAATA TGAAGACTGT AGACTGCGTG 
GAGCGCAAGG GCAAGTATAT GTACTTCACT GTGGTGATGG CGGAGGGCAA AGAGATCGAC 
TTTCGGTGTC CCCAGGACCA GGGCTGGAAC GCAGAGATCA CGTTGCAGAT GGTGCAGTAC 
AAAAATCGTC AGGCCATACT GGCGGTCAAG TCTACGAGGC AGAAGCAGCA GCACCTGGTC 
CAGCAGCAGC CTCCGCAGAC GCAGCAGATC CAACCCCAGC CCCAGCCGCA GATCCAACCC 
CAGCCCCAGC CGCAGATCCA GCCTCAACCC CAACCCCAAC CCCAACCTCA GCCCCAACCC 
CAGCAGCAGC CCCAGCCCCA ACAGCTCCAC TCCTACCCGC ACCCGCATCC TCATCTTTAC 
CCTCACCCTC ACCCTCACGC GCACTCGCAC CCGCACCCGC ACCCTCACCC TCATCCACAC 
CAACTCCAGC ATGCGCACCA GCCTCTCCAC TCGCAGCCAC AGGGCCACCG GCTTCTCCGC 
AGCACCTCCA ACTCTGCCTG A