Prkd2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Prkd2
Entrez Gene ID	292658
Full Name	protein kinase D2
Synonyms	RGD1308054
General protein information	Preferred Names protein kinase D2 Names serine/threonine-protein kinase D2 nPKC-D2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q21

mRNA and Protein(s)

mRNA	Protein	Name
NM_001013895.1	NP_001013917.1	serine/threonine-protein kinase D2

Pathways from BioSystems

Homologies

Homo sapiens (human)	PRKD2	NP_057541.2
Pan troglodytes (chimpanzee)	PRKD2	XP_524314.3
Rattus norvegicus (Norway rat)	Prkd2	NP_001013917.1
Mus musculus (house mouse)	Prkd2	NP_001239387.1
Bos taurus (cattle)	PRKD2	XP_005219507.1
Canis lupus familiaris (dog)	PRKD2	XP_005616468.1

Gene Ontology
Bibliography

Related articles in PubMed

Differential regulation of PKD isoforms in oxidative stress conditions through phosphorylation of a conserved Tyr in the P+1 loop.
Cobbaut M, Derua R, D?ppler H, Lou HJ, Vandoninck S, Storz P, Turk BE, Seufferlein T, Waelkens E, Janssens V, Van Lint J
Scientific reports7(1)887(2017 Apr)

Biphasic Regulation of Yes-associated Protein (YAP) Cellular Localization, Phosphorylation, and Activity by G Protein-coupled Receptor Agonists in Intestinal Epithelial Cells: A NOVEL ROLE FOR PROTEIN KINASE D (PKD).
Wang J, Sinnett-Smith J, Stevens JV, Young SH, Rozengurt E
The Journal of biological chemistry291(34)17988-8005(2016 08)

Protein kinase D regulates RhoA activity via rhotekin phosphorylation.
Pusapati GV, Eiseler T, Rykx A, Vandoninck S, Derua R, Waelkens E, Van Lint J, von Wichert G, Seufferlein T
The Journal of biological chemistry287(12)9473-83(2012 Mar)

Unique functions for protein kinase D1 and protein kinase D2 in mammalian cells.
Matthews SA, Navarro MN, Sinclair LV, Emslie E, Feijoo-Carnero C, Cantrell DA
The Biochemical journal432(1)153-63(2010 Nov)

Characterization of the biological effects of a novel protein kinase D inhibitor in endothelial cells.
Evans IM, Bagherzadeh A, Charles M, Raynham T, Ireson C, Boakes A, Kelland L, Zachary IC
The Biochemical journal429(3)565-72(2010 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

siRNA-mediated knockdown of PKD1, PKD2, and PKD3 markedly attenuated YAP nuclear-cytoplasmic shuttling, phosphorylation at Ser(127), and induction of Ctgf and Areg expression in response to GPCR activation.
Title: Biphasic Regulation of Yes-associated Protein (YAP) Cellular Localization, Phosphorylation, and Activity by G Protein-coupled Receptor Agonists in Intestinal Epithelial Cells: A NOVEL ROLE FOR PROTEIN KINASE D (PKD).

The following Prkd2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Prkd2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa04114	NM_001013895.1 Latest version!	Rattus norvegicus protein kinase D2 (Prkd2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$475.30 $679.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Prkd2 ( NM_001013895.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001013917.1 Rattus norvegicus protein kinase D2 (Prkd2), mRNA.

Price & Availability↑

CloneID	ORa04114
Clone ID Related Accession (Same CDS sequence)	NM_001013895.1
Accession Version	NM_001013895.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2628bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-16
Organism	Rattus norvegicus(Norway rat)
Product	serine/threonine-protein kinase D2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC083592.1. On Apr 20, 2005 this sequence version replaced XM_214834.3. Sequence Note: This sequence has been modified as follows: removed 36 bp suspected to be vector contamination from the 3' end. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC083592.1, FQ218070.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052297, SAMEA2689594 [ECO:0000348] ##Evidence-Data-END##

ATGGCCGCCG CCCCCTCCCA TCCCGCCGGA CTCCCCTGCT CTCCGGGGCC CGGGTCTCCT 
CCGCCCCCCG GTGGCTCGGA TCTCCAGTCT CTGCCACCTC TGCTGCCTCA GATCCCTGCT 
CCGGGATCGG GGGTCTCCTT CCACATCCAG ATCGGATTAA CACGAGAGTT TGTGCTGTTG 
CCGGCAGCTT CGGAGTTGGC TCATGTGAAG CAACTAGCCT GTTCTATCGT GGACCAGAAG 
TTCCCAGAGT GTGGCTTCTA CGGCCTGTAC GACAAGATCC TGCTCTTCAA ACATGACCCA 
ACATCAGCCA ACCTCCTGCA GCTGGTGCGA TCAGCTGCAG ATATCCAGGA GGGCGACTTG 
GTGGAGGTGG TTCTGTCGGC CTCAGCCACC TTCGAGGATT TCCAGATCCG TCCGCATGCT 
CTCACTGTGC ACTCGTACCG CGCACCAGCT TTCTGTGACC ACTGTGGGGA GATGCTGTTT 
GGCCTTGTGC GCCAGGGCCT CAAGTGCGAT GGCTGCGGGC TGAATTACCA CAAACGCTGC 
GCCTTCAGCA TCCCGAACAA CTGCAGTGGC GCTCGGAAGC GCAGACTGTC ATCCACGTCT 
CTGGCCAGTG GCCATTCTGT GCGTCTCGGC AGCTCCGAGT CCCTACCCTG CACCGCCGAA 
GAGCTGAGCC GTAGCACCAC CGACCTCCTT CCTCGCCGAC CACCCTCGTC GTCGTCCTCC 
TCCTCTTCCT CTTCCTTCTA CACAGGCCGA CCCATTGAGC TGGACAAGAT GCTGATGTCC 
AAGGTCAAGG TGCCACACAC CTTCCTTATA CATAGCTACA CACGGCCCAC CGTTTGCCAG 
GCTTGCAAGA AACTGCTCAA GGGTCTCTTC CGCCAGGGCC TGCAGTGCAA AGACTGCAAG 
TTTAACTGTC ACAAACGCTG TGCTACCCGA GTCCCTAATG ACTGCCTGGG GGAGGCGCTC 
ATCAATGGAG ATGTGCCCAT GGAAGAAGCT GCTGATTACA GTGAGGCTGA TAAGAGCTCC 
CTCTCAGATG AGTTGGAGGA CTCTGGTGTC ATCCCAGGCT CCCATGCAGA GAATGCTCTC 
CATGCCAGTG AGGAAGAGGA AGGCGAGGGA GGCAAGGCCC AGAGCTCGCT GGGCTACATC 
CCCCTGATGC GTGTGGTACA GTCTGTGCGA CACACGACCC GGAAATCCAG CACCACCCTG 
CGGGAGGGCT GGGTGGTCCA TTACAGCAAC AAGGACACAC TGAGGAAGCG GCACTACTGG 
CGCCTAGACT GCAAATGCAT TACCCTCTTC CAGAACAACA CGACCAACAG ATACTACAAG 
GAGATCCCGC TGTCTGAAAT CCTCGCAGTG GAGCCGGCTC AGAACTTCAG CCTCGTACCT 
CCGGGCACCA ACCCACACTG CTTTGAGATC ATCACCGCCA ATGTCACCTA CTTTGTGGGC 
GAGACACCTG GCGGGGCCCC GGGAGGGCCG AGCGGGCAGG GAACAGAGGC TGCCCGGGGT 
TGGGAGACAG CCATCCGCCA GGCACTGATG CCTGTTATCC TCCAAGATGC ACCCAGTGCC 
CCAGGCCACA CACCACACAG ACAAGCTTCT CTGAGCATCT CTGTGTCCAA CAGTCAGATC 
CAAGAGAACG TGGACATCGC CACTGTCTAC CAGATCTTCC CCGATGAGGT GCTGGGCTCT 
GGACAGTTTG GAGTGGTCTA TGGAGGAAAA CACAGGAAGA CTGGCAGAGA TGTTGCAGTA 
AAGGTCATTG ACAAACTACG CTTCCCCACC AAGCAGGAGA GCCAGCTCAG GAATGAAGTG 
GCCATCCTCC AGAGCCTGCG GCACCCCGGG ATTGTGAACC TCGAGTGCAT GTTTGAGACC 
CCTGAGAAGG TATTCGTGGT GATGGAGAAA CTGCATGGGG ACATGTTGGA GATGATCCTC 
TCCAGTGAGA AGGGCCGGCT TCCTGAGCGC CTCACCAAGT TCCTTATCAC GCAGATTCTG 
GTGGCACTGA GACACCTACA CTTCAAGAAC ATCGTCCACT GTGACTTGAA ACCAGAGAAT 
GTGTTACTGG CATCAGCCGA TCCGTTTCCT CAGGTGAAGC TATGTGACTT CGGCTTCGCT 
CGCATCATCG GTGAGAAGTC CTTCCGGCGC TCAGTGGTGG GCACGCCCGC CTACCTGGCA 
CCTGAGGTAC TGCTCAACCA GGGTTACAAC CGCTCGCTGG ACATGTGGTC CGTGGGTGTG 
ATCATGTATG TGAGCCTCAG TGGCACGTTT CCCTTCAATG AGGACGAGGA CATCAATGAC 
CAGATACAGA ATGCAGCCTT TATGTACCCG GCCAGCCCCT GGAGCCACAT CTCATCTGGA 
GCCATCGACC TCATCAACAA CCTGTTGCAG GTGAAGATGC GCAAGCGCTA CAGCGTGGAC 
AAGTCTCTCA GCCACCCATG GTTACAGGAG TACCAGACGT GGCTTGACCT CCGAGAGCTA 
GAGGGAAAGA TGGGCGAGCG ATATATCACC CATGAGAGCG ACGACGCACG CTGGGATCAG 
TTTGTATCAG AGCGCCATGG GACGCCTGCA GAAGGGGACC TGGGTGGCGC CTGTCTGCCA 
CAGGACCATG AGATGCAGGG GCTGGCTGAG CGGATCAGCA TCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001013917.1
CDS	157..2784
Translation	MAAAPSHPAGLPCSPGPGSPPPPGGSDLQSLPPLLPQIPAPGSGVSFHIQIGLTREFVLLPAASELAHVKQLACSIVDQKFPECGFYGLYDKILLFKHDPTSANLLQLVRSAADIQEGDLVEVVLSASATFEDFQIRPHALTVHSYRAPAFCDHCGEMLFGLVRQGLKCDGCGLNYHKRCAFSIPNNCSGARKRRLSSTSLASGHSVRLGSSESLPCTAEELSRSTTDLLPRRPPSSSSSSSSSSFYTGRPIELDKMLMSKVKVPHTFLIHSYTRPTVCQACKKLLKGLFRQGLQCKDCKFNCHKRCATRVPNDCLGEALINGDVPMEEAADYSEADKSSLSDELEDSGVIPGSHAENALHASEEEEGEGGKAQSSLGYIPLMRVVQSVRHTTRKSSTTLREGWVVHYSNKDTLRKRHYWRLDCKCITLFQNNTTNRYYKEIPLSEILAVEPAQNFSLVPPGTNPHCFEIITANVTYFVGETPGGAPGGPSGQGTEAARGWETAIRQALMPVILQDAPSAPGHTPHRQASLSISVSNSQIQENVDIATVYQIFPDEVLGSGQFGVVYGGKHRKTGRDVAVKVIDKLRFPTKQESQLRNEVAILQSLRHPGIVNLECMFETPEKVFVVMEKLHGDMLEMILSSEKGRLPERLTKFLITQILVALRHLHFKNIVHCDLKPENVLLASADPFPQVKLCDFGFARIIGEKSFRRSVVGTPAYLAPEVLLNQGYNRSLDMWSVGVIMYVSLSGTFPFNEDEDINDQIQNAAFMYPASPWSHISSGAIDLINNLLQVKMRKRYSVDKSLSHPWLQEYQTWLDLRELEGKMGERYITHESDDARWDQFVSERHGTPAEGDLGGACLPQDHEMQGLAERISIL

Target ORF information:

RefSeq Version	NM_001013895.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus protein kinase D2 (Prkd2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001013895.1

ORF Insert Sequence:

ATGGCCGCCG CCCCCTCCCA TCCCGCCGGA CTCCCCTGCT CTCCGGGGCC CGGGTCTCCT 
CCGCCCCCCG GTGGCTCGGA TCTCCAGTCT CTGCCACCTC TGCTGCCTCA GATCCCTGCT 
CCGGGATCGG GGGTCTCCTT CCACATCCAG ATCGGATTAA CACGAGAGTT TGTGCTGTTG 
CCGGCAGCTT CGGAGTTGGC TCATGTGAAG CAACTAGCCT GTTCTATCGT GGACCAGAAG 
TTCCCAGAGT GTGGCTTCTA CGGCCTGTAC GACAAGATCC TGCTCTTCAA ACATGACCCA 
ACATCAGCCA ACCTCCTGCA GCTGGTGCGA TCAGCTGCAG ATATCCAGGA GGGCGACTTG 
GTGGAGGTGG TTCTGTCGGC CTCAGCCACC TTCGAGGATT TCCAGATCCG TCCGCATGCT 
CTCACTGTGC ACTCGTACCG CGCACCAGCT TTCTGTGACC ACTGTGGGGA GATGCTGTTT 
GGCCTTGTGC GCCAGGGCCT CAAGTGCGAT GGCTGCGGGC TGAATTACCA CAAACGCTGC 
GCCTTCAGCA TCCCGAACAA CTGCAGTGGC GCTCGGAAGC GCAGACTGTC ATCCACGTCT 
CTGGCCAGTG GCCATTCTGT GCGTCTCGGC AGCTCCGAGT CCCTACCCTG CACCGCCGAA 
GAGCTGAGCC GTAGCACCAC CGACCTCCTT CCTCGCCGAC CACCCTCGTC GTCGTCCTCC 
TCCTCTTCCT CTTCCTTCTA CACAGGCCGA CCCATTGAGC TGGACAAGAT GCTGATGTCC 
AAGGTCAAGG TGCCACACAC CTTCCTTATA CATAGCTACA CACGGCCCAC CGTTTGCCAG 
GCTTGCAAGA AACTGCTCAA GGGTCTCTTC CGCCAGGGCC TGCAGTGCAA AGACTGCAAG 
TTTAACTGTC ACAAACGCTG TGCTACCCGA GTCCCTAATG ACTGCCTGGG GGAGGCGCTC 
ATCAATGGAG ATGTGCCCAT GGAAGAAGCT GCTGATTACA GTGAGGCTGA TAAGAGCTCC 
CTCTCAGATG AGTTGGAGGA CTCTGGTGTC ATCCCAGGCT CCCATGCAGA GAATGCTCTC 
CATGCCAGTG AGGAAGAGGA AGGCGAGGGA GGCAAGGCCC AGAGCTCGCT GGGCTACATC 
CCCCTGATGC GTGTGGTACA GTCTGTGCGA CACACGACCC GGAAATCCAG CACCACCCTG 
CGGGAGGGCT GGGTGGTCCA TTACAGCAAC AAGGACACAC TGAGGAAGCG GCACTACTGG 
CGCCTAGACT GCAAATGCAT TACCCTCTTC CAGAACAACA CGACCAACAG ATACTACAAG 
GAGATCCCGC TGTCTGAAAT CCTCGCAGTG GAGCCGGCTC AGAACTTCAG CCTCGTACCT 
CCGGGCACCA ACCCACACTG CTTTGAGATC ATCACCGCCA ATGTCACCTA CTTTGTGGGC 
GAGACACCTG GCGGGGCCCC GGGAGGGCCG AGCGGGCAGG GAACAGAGGC TGCCCGGGGT 
TGGGAGACAG CCATCCGCCA GGCACTGATG CCTGTTATCC TCCAAGATGC ACCCAGTGCC 
CCAGGCCACA CACCACACAG ACAAGCTTCT CTGAGCATCT CTGTGTCCAA CAGTCAGATC 
CAAGAGAACG TGGACATCGC CACTGTCTAC CAGATCTTCC CCGATGAGGT GCTGGGCTCT 
GGACAGTTTG GAGTGGTCTA TGGAGGAAAA CACAGGAAGA CTGGCAGAGA TGTTGCAGTA 
AAGGTCATTG ACAAACTACG CTTCCCCACC AAGCAGGAGA GCCAGCTCAG GAATGAAGTG 
GCCATCCTCC AGAGCCTGCG GCACCCCGGG ATTGTGAACC TCGAGTGCAT GTTTGAGACC 
CCTGAGAAGG TATTCGTGGT GATGGAGAAA CTGCATGGGG ACATGTTGGA GATGATCCTC 
TCCAGTGAGA AGGGCCGGCT TCCTGAGCGC CTCACCAAGT TCCTTATCAC GCAGATTCTG 
GTGGCACTGA GACACCTACA CTTCAAGAAC ATCGTCCACT GTGACTTGAA ACCAGAGAAT 
GTGTTACTGG CATCAGCCGA TCCGTTTCCT CAGGTGAAGC TATGTGACTT CGGCTTCGCT 
CGCATCATCG GTGAGAAGTC CTTCCGGCGC TCAGTGGTGG GCACGCCCGC CTACCTGGCA 
CCTGAGGTAC TGCTCAACCA GGGTTACAAC CGCTCGCTGG ACATGTGGTC CGTGGGTGTG 
ATCATGTATG TGAGCCTCAG TGGCACGTTT CCCTTCAATG AGGACGAGGA CATCAATGAC 
CAGATACAGA ATGCAGCCTT TATGTACCCG GCCAGCCCCT GGAGCCACAT CTCATCTGGA 
GCCATCGACC TCATCAACAA CCTGTTGCAG GTGAAGATGC GCAAGCGCTA CAGCGTGGAC 
AAGTCTCTCA GCCACCCATG GTTACAGGAG TACCAGACGT GGCTTGACCT CCGAGAGCTA 
GAGGGAAAGA TGGGCGAGCG ATATATCACC CATGAGAGCG ACGACGCACG CTGGGATCAG 
TTTGTATCAG AGCGCCATGG GACGCCTGCA GAAGGGGACC TGGGTGGCGC CTGTCTGCCA 
CAGGACCATG AGATGCAGGG GCTGGCTGAG CGGATCAGCA TCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Differential regulation of PKD isoforms in oxidative stress conditions through phosphorylation of a conserved Tyr in the P+1 loop.
Scientific reports7(1)887(2017 Apr)
Cobbaut M,Derua R,D?ppler H,Lou HJ,Vandoninck S,Storz P,Turk BE,Seufferlein T,Waelkens E,Janssens V,Van Lint J

Biphasic Regulation of Yes-associated Protein (YAP) Cellular Localization, Phosphorylation, and Activity by G Protein-coupled Receptor Agonists in Intestinal Epithelial Cells: A NOVEL ROLE FOR PROTEIN KINASE D (PKD).
The Journal of biological chemistry291(34)17988-8005(2016 08)
Wang J,Sinnett-Smith J,Stevens JV,Young SH,Rozengurt E

Protein kinase D regulates RhoA activity via rhotekin phosphorylation.
The Journal of biological chemistry287(12)9473-83(2012 Mar)
Pusapati GV,Eiseler T,Rykx A,Vandoninck S,Derua R,Waelkens E,Van Lint J,von Wichert G,Seufferlein T

Unique functions for protein kinase D1 and protein kinase D2 in mammalian cells.
The Biochemical journal432(1)153-63(2010 Nov)
Matthews SA,Navarro MN,Sinclair LV,Emslie E,Feijoo-Carnero C,Cantrell DA

Characterization of the biological effects of a novel protein kinase D inhibitor in endothelial cells.
The Biochemical journal429(3)565-72(2010 Aug)
Evans IM,Bagherzadeh A,Charles M,Raynham T,Ireson C,Boakes A,Kelland L,Zachary IC

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Prkd2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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