Ptma cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ptma
Entrez Gene ID	29222
Full Name	prothymosin alpha
General protein information	Preferred Names prothymosin alpha Names prothymosin alpha alpha-prothymosin
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 9 Map Location: 9q35
Summary	human homolog plays a role in anti-apoptosis [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_021740.1	NP_068508.1	prothymosin alpha

Pathways from BioSystems

Homologies

	LOC702864	XP_001096350.1
Canis lupus familiaris (dog)	LOC611335	XP_003435633.1
Rattus norvegicus (Norway rat)	Ptma	NP_068508.1
Rattus norvegicus (Norway rat)	LOC100359583	XP_003751326.1

Gene Ontology
Bibliography

Related articles in PubMed

First evidence of prothymosin ? localization in the acrosome of mammalian male gametes.
Ferrara D, Pariante P, Di Matteo L, Serino I, Oko R, Minucci S
Journal of cellular physiology228(8)1629-37(2013 Aug)

Novel neuroprotective action of prothymosin ?-derived peptide against retinal and brain ischemic damages.
Halder SK, Matsunaga H, Yamaguchi H, Ueda H
Journal of neurochemistry125(5)713-23(2013 Jun)

Stress-induced non-vesicular release of prothymosin-? initiated by an interaction with S100A13, and its blockade by caspase-3 cleavage.
Matsunaga H, Ueda H
Cell death and differentiation17(11)1760-72(2010 Nov)

Expression of prothymosin alpha in meiotic and post-meiotic germ cells during the first wave of rat spermatogenesis.
Ferrara D, Izzo G, Pariante P, Donizetti A, d'Istria M, Aniello F, Minucci S
Journal of cellular physiology224(2)362-8(2010 Aug)

Prothymosin alpha is a component of a linker histone chaperone.
George EM, Brown DT
FEBS letters584(13)2833-6(2010 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

PTMA is specifically localized in the acrosome of epididymal sperm
Title: First evidence of prothymosin ? localization in the acrosome of mammalian male gametes.

The active core peptide sequence P30 (amino acids 49-78) of prothymosin alpha exerts its full survival effect in cultured cortical neurons against ischemic stress.
Title: Novel neuroprotective action of prothymosin ?-derived peptide against retinal and brain ischemic damages.

results suggest necrosis-inducing stress induces an extacellular release of prothymosin-alpha in a non-vesicular manner; apoptosis-inducing stress does not, owing to loss of its interaction with S100A13, a cargo molecule for extracellular release
Title: Stress-induced non-vesicular release of prothymosin-? initiated by an interaction with S100A13, and its blockade by caspase-3 cleavage.

PTMA specific localization in the heads of spermatids and epididymal spermatozoa, associated with the acrosome system, supports for the first time the hypothesis of a direct function in male germ cells.
Title: Expression of prothymosin alpha in meiotic and post-meiotic germ cells during the first wave of rat spermatogenesis.

The differences in prothymosin alpha expression and localization during hepatocyte proliferation and apoptosis suggest that this protein may have a pleiotropic function.
Title: Apoptotic and proliferating hepatocytes differ in prothymosin alpha expression and cell localization.

The following Ptma gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ptma cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa05126	NM_021740.1 Latest version!	Rattus norvegicus prothymosin alpha (Ptma), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ptma ( NM_021740.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_068508.1 Rattus norvegicus prothymosin alpha (Ptma), mRNA.

Price & Availability↑

CloneID	ORa05126
Clone ID Related Accession (Same CDS sequence)	NM_021740.1
Accession Version	NM_021740.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 339bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Rattus norvegicus(Norway rat)
Product	prothymosin alpha
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from M86564.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M86564.1, BC061972.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGTCAGACG CGGCAGTGGA CACCAGCTCC GAGATCACCA CCAAGGACTT GAAGGAGAAG 
AAGGAAGTTG TGGAGGAGGC AGAGAATGGA AGAGACGCAC CTGCCAATGG GAACGCTCAA 
AATGAGGAAA ATGGGGAGCA GGAGGCTGAC AATGAGGTAG ATGAAGAAGA GGAAGAAGGT 
GGGGAGGAAG AGGAGGAGGA GGAAGAAGGT GATGGTGAGG AAGAAGATGG AGATGAAGAT 
GAGGAAGCTG AGGCTCCTAC GGGCAAGCGG GTAGCTGAGG ATGATGAGGA TGATGATGTT 
GAGACCAAGA AGCAGAAGAA GACTGATGAG GATGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_068508.1
CDS	147..485
Translation	MSDAAVDTSSEITTKDLKEKKEVVEEAENGRDAPANGNAQNEENGEQEADNEVDEEEEEGGEEEEEEEEGDGEEEDGDEDEEAEAPTGKRVAEDDEDDDVETKKQKKTDEDD

Target ORF information:

RefSeq Version	NM_021740.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus prothymosin alpha (Ptma), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021740.1

ORF Insert Sequence:

ATGTCAGACG CGGCAGTGGA CACCAGCTCC GAGATCACCA CCAAGGACTT GAAGGAGAAG 
AAGGAAGTTG TGGAGGAGGC AGAGAATGGA AGAGACGCAC CTGCCAATGG GAACGCTCAA 
AATGAGGAAA ATGGGGAGCA GGAGGCTGAC AATGAGGTAG ATGAAGAAGA GGAAGAAGGT 
GGGGAGGAAG AGGAGGAGGA GGAAGAAGGT GATGGTGAGG AAGAAGATGG AGATGAAGAT 
GAGGAAGCTG AGGCTCCTAC GGGCAAGCGG GTAGCTGAGG ATGATGAGGA TGATGATGTT 
GAGACCAAGA AGCAGAAGAA GACTGATGAG GATGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

First evidence of prothymosin ? localization in the acrosome of mammalian male gametes.
Journal of cellular physiology228(8)1629-37(2013 Aug)
Ferrara D,Pariante P,Di Matteo L,Serino I,Oko R,Minucci S

Novel neuroprotective action of prothymosin ?-derived peptide against retinal and brain ischemic damages.
Journal of neurochemistry125(5)713-23(2013 Jun)
Halder SK,Matsunaga H,Yamaguchi H,Ueda H

Stress-induced non-vesicular release of prothymosin-? initiated by an interaction with S100A13, and its blockade by caspase-3 cleavage.
Cell death and differentiation17(11)1760-72(2010 Nov)
Matsunaga H,Ueda H

Expression of prothymosin alpha in meiotic and post-meiotic germ cells during the first wave of rat spermatogenesis.
Journal of cellular physiology224(2)362-8(2010 Aug)
Ferrara D,Izzo G,Pariante P,Donizetti A,d'Istria M,Aniello F,Minucci S

Prothymosin alpha is a component of a linker histone chaperone.
FEBS letters584(13)2833-6(2010 Jul)
George EM,Brown DT