Hs2st1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Hs2st1
Entrez Gene ID	292155
Full Name	heparan sulfate 2-O-sulfotransferase 1
General protein information	Preferred Names heparan sulfate 2-O-sulfotransferase 1 Names heparan sulfate 2-O-sulfotransferase 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 2 Map Location: 2q44

mRNA and Protein(s)

mRNA	Protein	Name
XM_008761495.2	XP_008759717.1	heparan sulfate 2-O-sulfotransferase 1 isoform X1
NM_001100518.1	NP_001093988.1	heparan sulfate 2-O-sulfotransferase 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	HS2ST1	NP_036394.1
Bos taurus (cattle)	HS2ST1	XP_005204478.1
Danio rerio (zebrafish)	hs2st1a	NP_001074139.1
	HS2ST1	XP_001109871.1
Mus musculus (house mouse)	Hs2st1	NP_035958.3
Xenopus tropicalis (tropical clawed frog)	hs2st1	NP_001120463.1
Rattus norvegicus (Norway rat)	Hs2st1	NP_001093988.1
Gallus gallus (chicken)	HS2ST1	NP_989812.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008166	XP_317295.4
Canis lupus familiaris (dog)	HS2ST1	XP_005622019.1
Pan troglodytes (chimpanzee)	HS2ST1	XP_524759.2
Drosophila melanogaster (fruit fly)	Hs2st	NP_477339.1

Gene Ontology
Bibliography

Related articles in PubMed

Defining the membrane proteome of NK cells.
Ghosh D, Lippert D, Krokhin O, Cortens JP, Wilkins JA
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)

The molecular phenotype of heparan sulfate in the Hs2st-/- mutant mouse.
Merry CL, Bullock SL, Swan DC, Backen AC, Lyon M, Beddington RS, Wilson VA, Gallagher JT
The Journal of biological chemistry276(38)35429-34(2001 Sep)

Renal agenesis in mice homozygous for a gene trap mutation in the gene encoding heparan sulfate 2-sulfotransferase.
Bullock SL, Fletcher JM, Beddington RS, Wilson VA
Genes & development12(12)1894-906(1998 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Hs2st1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hs2st1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa00366	NM_001100518.1 Latest version!	Rattus norvegicus heparan sulfate 2-O-sulfotransferase 1 (Hs2st1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
ORa45032	XM_008761495.2 Latest version!	Rattus norvegicus heparan sulfate 2-O-sulfotransferase 1 (Hs2st1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Hs2st1 ( NM_001100518.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001093988.1 Rattus norvegicus heparan sulfate 2-O-sulfotransferase 1 (Hs2st1), mRNA.

Price & Availability↑

CloneID	ORa00366
Clone ID Related Accession (Same CDS sequence)	NM_001100518.1
Accession Version	NM_001100518.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1071bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-27
Organism	Rattus norvegicus(Norway rat)
Product	heparan sulfate 2-O-sulfotransferase 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY724508.1, BC091291.1, FM092358.1, DY318006.1, FM104316.1, BQ194342.1 and CK839839.1. On or before Nov 22, 2008 this sequence version replaced XM_214740.4, XM_001079125.1. Sequence Note: This RefSeq record was created from transcript data of mixed strains because no single transcript was available for the full length of the gene. The extent of this transcript is supported by alignment data. ##Evidence-Data-START## Transcript exon combination :: AY724508.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01906349 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGCTCC TCAGGATTAT GATGCCGCCC AAGTTGCAGC TGCTGGCGGT GGTGGCCTTC 
GCGGTGGCGA TGCTCTTCTT GGAGAACCAG ATCCAGAAGC TGGAGGAGTC CCGGGCGAAG 
CTAGAGAGGG CAATTGCAAG ACATGAAGTC CGAGAGATTG AACAGCGCCA CACGATGGAC 
GGCCCTCGGC AGGATGCCAC TCTGGATGAA GAAGAAGACA TCGTCATCAT TTATAACAGA 
GTGCCCAAAA CTGCAAGCAC CTCGTTCACC AATATCGCCT ATGACCTGTG TGCAAAGAAT 
AGATACCACG TTCTTCACAT CAACACTACC AAAAACAACC CAGTAATGTC ATTGCAGGAT 
CAGGTGCGCT TTGTAAAGAA TATAACCAGC TGGAACGAGA TGAAACCAGG CTTCTATCAC 
GGACACATTT CTTACTTGGA TTTTGCAAAA TTCGGGGTGA AGAAGAAGCC GATTTACATC 
AATGTCGTCA GGGACCCGAT TGAGAGGCTA GTTTCCTACT ATTACTTTCT GAGGTTTGGA 
GATGATTACA GACCGGGACT AAGGAGGCGG AAACAAGGAG ACAAGAAGAC CTTCGATGAA 
TGCGTGGCTG AGGGCGGCTC AGACTGTGCT CCAGAGAAGC TCTGGCTTCA GATCCCGTTC 
TTCTGTGGCC ACAGCTCAGA ATGCTGGAAT GTTGGAAGCA GATGGGCGAT GGATCAAGCT 
AAGTACAACC TAGTTAATGA GTACTTCCTG GTGGGGGTTA CTGAAGAGTT GGAAGACTTC 
ATCATGCTCC TGGAGGCGGC TCTGCCCCGG TTCTTCAGGG GCGCGACGGA CCTCTATCGT 
ACAGGAAAGA AATCTCACCT TAGGAAAACT ACAGAGAAGA AACTGCCCAC TAAACAGACC 
ATCGCGAAGC TACAGCAGTC CGACATTTGG AAAATGGAGA ATGAGTTCTA TGAGTTTGCC 
CTGGAGCAGT TCCAGTTCAT GAGAGCCCAC GCGGTCCGTG AGAAAGATGG AGACCTCTAC 
ATCCTGGCCC AGAACTTTTT CTATGAAAAG ATTTACCCTA AGTCGAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001093988.1
CDS	279..1349
Translation	MGLLRIMMPPKLQLLAVVAFAVAMLFLENQIQKLEESRAKLERAIARHEVREIEQRHTMDGPRQDATLDEEEDIVIIYNRVPKTASTSFTNIAYDLCAKNRYHVLHINTTKNNPVMSLQDQVRFVKNITSWNEMKPGFYHGHISYLDFAKFGVKKKPIYINVVRDPIERLVSYYYFLRFGDDYRPGLRRRKQGDKKTFDECVAEGGSDCAPEKLWLQIPFFCGHSSECWNVGSRWAMDQAKYNLVNEYFLVGVTEELEDFIMLLEAALPRFFRGATDLYRTGKKSHLRKTTEKKLPTKQTIAKLQQSDIWKMENEFYEFALEQFQFMRAHAVREKDGDLYILAQNFFYEKIYPKSN

Target ORF information:

RefSeq Version	NM_001100518.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus heparan sulfate 2-O-sulfotransferase 1 (Hs2st1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001100518.1

ORF Insert Sequence:

ATGGGGCTCC TCAGGATTAT GATGCCGCCC AAGTTGCAGC TGCTGGCGGT GGTGGCCTTC 
GCGGTGGCGA TGCTCTTCTT GGAGAACCAG ATCCAGAAGC TGGAGGAGTC CCGGGCGAAG 
CTAGAGAGGG CAATTGCAAG ACATGAAGTC CGAGAGATTG AACAGCGCCA CACGATGGAC 
GGCCCTCGGC AGGATGCCAC TCTGGATGAA GAAGAAGACA TCGTCATCAT TTATAACAGA 
GTGCCCAAAA CTGCAAGCAC CTCGTTCACC AATATCGCCT ATGACCTGTG TGCAAAGAAT 
AGATACCACG TTCTTCACAT CAACACTACC AAAAACAACC CAGTAATGTC ATTGCAGGAT 
CAGGTGCGCT TTGTAAAGAA TATAACCAGC TGGAACGAGA TGAAACCAGG CTTCTATCAC 
GGACACATTT CTTACTTGGA TTTTGCAAAA TTCGGGGTGA AGAAGAAGCC GATTTACATC 
AATGTCGTCA GGGACCCGAT TGAGAGGCTA GTTTCCTACT ATTACTTTCT GAGGTTTGGA 
GATGATTACA GACCGGGACT AAGGAGGCGG AAACAAGGAG ACAAGAAGAC CTTCGATGAA 
TGCGTGGCTG AGGGCGGCTC AGACTGTGCT CCAGAGAAGC TCTGGCTTCA GATCCCGTTC 
TTCTGTGGCC ACAGCTCAGA ATGCTGGAAT GTTGGAAGCA GATGGGCGAT GGATCAAGCT 
AAGTACAACC TAGTTAATGA GTACTTCCTG GTGGGGGTTA CTGAAGAGTT GGAAGACTTC 
ATCATGCTCC TGGAGGCGGC TCTGCCCCGG TTCTTCAGGG GCGCGACGGA CCTCTATCGT 
ACAGGAAAGA AATCTCACCT TAGGAAAACT ACAGAGAAGA AACTGCCCAC TAAACAGACC 
ATCGCGAAGC TACAGCAGTC CGACATTTGG AAAATGGAGA ATGAGTTCTA TGAGTTTGCC 
CTGGAGCAGT TCCAGTTCAT GAGAGCCCAC GCGGTCCGTG AGAAAGATGG AGACCTCTAC 
ATCCTGGCCC AGAACTTTTT CTATGAAAAG ATTTACCCTA AGTCGAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hs2st1 ( XM_008761495.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008759717.1 Rattus norvegicus heparan sulfate 2-O-sulfotransferase 1 (Hs2st1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa45032
Clone ID Related Accession (Same CDS sequence)	XM_008761495.2
Accession Version	XM_008761495.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1074bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	heparan sulfate 2-O-sulfotransferase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008761495.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCTCC TCAGGATTAT GATGCCGCCC AAGTTGCAGC TGCTGGCGGT GGTGGCCTTC 
GCGGTGGCGA TGCTCTTCTT GGAGAACCAG ATCCAGAAGC TGGAGGAGTC CCGGGCGAAG 
CTAGAGAGGG CAATTGCAAG ACATGAAGTC CGAGAGATTG AACAGCGCCA CACGATGGAC 
GGCCCTCGGC AGGATGCCAC TCTGGATGAA GAAGAAGACA TCGTCATCAT TTATAACAGA 
GTGCCCAAAA CTGCAAGCAC CTCGTTCACC AATATCGCCT ATGACCTGTG TGCAAAGAAT 
AGATACCACG TTCTTCACAT CAACACTACC AAAAACAACC CAGTAATGTC ATTGCAGGAT 
CAGGTGCGCT TTGTAAAGAA TATAACCAGC TGGAACGAGA TGAAACCAGG CTTCTATCAC 
GGACACATTT CTTACTTGGA TTTTGCAAAA TTCGGGGTGA AGAAGAAGCC GATTTACATC 
AATGTCGTCA GGGACCCGAT TGAGAGGCTA GTTTCCTACT ATTACTTTCT GAGGTTTGGA 
GATGATTACA GACCGGGACT AAGGAGGCGG AAACAAGGAG ACAAGAAGAC CTTCGATGAA 
TGCGTGGCTG AGGGCGGCTC AGACTGTGCT CCAGAGAAGC TCTGGCTTCA GATCCCGTTC 
TTCTGTGGCC ACAGCTCAGA ATGCTGGAAT GTTGGAAGCA GATGGGCGAT GGATCAAGCT 
AAGTACAACC TAGTTAATGA GTACTTCCTG GTGGGGGTTA CTGAAGAGTT GGAAGACTTC 
ATCATGCTCC TGGAGGCGGC TCTGCCCCGG TTCTTCAGGG GCGCGACGGA CCTCTATCGT 
ACAGTAGGAA AGAAATCTCA CCTTAGGAAA ACTACAGAGA AGAAACTGCC CACTAAACAG 
ACCATCGCGA AGCTACAGCA GTCCGACATT TGGAAAATGG AGAATGAGTT CTATGAGTTT 
GCCCTGGAGC AGTTCCAGTT CATGAGAGCC CACGCGGTCC GTGAGAAAGA TGGAGACCTC 
TACATCCTGG CCCAGAACTT TTTCTATGAA AAGATTTACC CTAAGTCGAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008759717.1
CDS	275..1348
Translation	MGLLRIMMPPKLQLLAVVAFAVAMLFLENQIQKLEESRAKLERAIARHEVREIEQRHTMDGPRQDATLDEEEDIVIIYNRVPKTASTSFTNIAYDLCAKNRYHVLHINTTKNNPVMSLQDQVRFVKNITSWNEMKPGFYHGHISYLDFAKFGVKKKPIYINVVRDPIERLVSYYYFLRFGDDYRPGLRRRKQGDKKTFDECVAEGGSDCAPEKLWLQIPFFCGHSSECWNVGSRWAMDQAKYNLVNEYFLVGVTEELEDFIMLLEAALPRFFRGATDLYRTVGKKSHLRKTTEKKLPTKQTIAKLQQSDIWKMENEFYEFALEQFQFMRAHAVREKDGDLYILAQNFFYEKIYPKSN

Target ORF information:

RefSeq Version	XM_008761495.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus heparan sulfate 2-O-sulfotransferase 1 (Hs2st1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008761495.2

ORF Insert Sequence:

ATGGGGCTCC TCAGGATTAT GATGCCGCCC AAGTTGCAGC TGCTGGCGGT GGTGGCCTTC 
GCGGTGGCGA TGCTCTTCTT GGAGAACCAG ATCCAGAAGC TGGAGGAGTC CCGGGCGAAG 
CTAGAGAGGG CAATTGCAAG ACATGAAGTC CGAGAGATTG AACAGCGCCA CACGATGGAC 
GGCCCTCGGC AGGATGCCAC TCTGGATGAA GAAGAAGACA TCGTCATCAT TTATAACAGA 
GTGCCCAAAA CTGCAAGCAC CTCGTTCACC AATATCGCCT ATGACCTGTG TGCAAAGAAT 
AGATACCACG TTCTTCACAT CAACACTACC AAAAACAACC CAGTAATGTC ATTGCAGGAT 
CAGGTGCGCT TTGTAAAGAA TATAACCAGC TGGAACGAGA TGAAACCAGG CTTCTATCAC 
GGACACATTT CTTACTTGGA TTTTGCAAAA TTCGGGGTGA AGAAGAAGCC GATTTACATC 
AATGTCGTCA GGGACCCGAT TGAGAGGCTA GTTTCCTACT ATTACTTTCT GAGGTTTGGA 
GATGATTACA GACCGGGACT AAGGAGGCGG AAACAAGGAG ACAAGAAGAC CTTCGATGAA 
TGCGTGGCTG AGGGCGGCTC AGACTGTGCT CCAGAGAAGC TCTGGCTTCA GATCCCGTTC 
TTCTGTGGCC ACAGCTCAGA ATGCTGGAAT GTTGGAAGCA GATGGGCGAT GGATCAAGCT 
AAGTACAACC TAGTTAATGA GTACTTCCTG GTGGGGGTTA CTGAAGAGTT GGAAGACTTC 
ATCATGCTCC TGGAGGCGGC TCTGCCCCGG TTCTTCAGGG GCGCGACGGA CCTCTATCGT 
ACAGTAGGAA AGAAATCTCA CCTTAGGAAA ACTACAGAGA AGAAACTGCC CACTAAACAG 
ACCATCGCGA AGCTACAGCA GTCCGACATT TGGAAAATGG AGAATGAGTT CTATGAGTTT 
GCCCTGGAGC AGTTCCAGTT CATGAGAGCC CACGCGGTCC GTGAGAAAGA TGGAGACCTC 
TACATCCTGG CCCAGAACTT TTTCTATGAA AAGATTTACC CTAAGTCGAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Defining the membrane proteome of NK cells.
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)
Ghosh D,Lippert D,Krokhin O,Cortens JP,Wilkins JA

The molecular phenotype of heparan sulfate in the Hs2st-/- mutant mouse.
The Journal of biological chemistry276(38)35429-34(2001 Sep)
Merry CL,Bullock SL,Swan DC,Backen AC,Lyon M,Beddington RS,Wilson VA,Gallagher JT

Renal agenesis in mice homozygous for a gene trap mutation in the gene encoding heparan sulfate 2-sulfotransferase.
Genes & development12(12)1894-906(1998 Jun)
Bullock SL,Fletcher JM,Beddington RS,Wilson VA