Cartpt cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cartpt
Entrez Gene ID	29131
Full Name	CART prepropeptide
Synonyms	Cart
General protein information	Preferred Names CART prepropeptide Names cocaine- and amphetamine-regulated transcript protein cocaine and amphetamine regulated transcript
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 2 Map Location: 2q12
Summary	neuronal protein that may play a role in brain development and may be regulated by testosterone [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006231843.3	XP_006231905.1	cocaine- and amphetamine-regulated transcript protein isoform X1
NM_017110.1	NP_058806.1	cocaine- and amphetamine-regulated transcript protein precursor

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Cartpt	NP_058806.1
Xenopus tropicalis (tropical clawed frog)	cartpt	XP_002934864.1
Bos taurus (cattle)	CARTPT	NP_001007821.1
Mus musculus (house mouse)	Cartpt	NP_038760.3
	CARTPT	XP_001096906.1
Canis lupus familiaris (dog)	CARTPT	XP_852772.1
Pan troglodytes (chimpanzee)	CARTPT	XP_001155069.1
Homo sapiens (human)	CARTPT	NP_004282.1

Gene Ontology
Bibliography

Related articles in PubMed

Impaired hypothalamic cocaine- and amphetamine-regulated transcript expression in lateral hypothalamic area and paraventricular nuclei of dehydration-induced anorexic rats.
Garc?a-Luna C, Soberanes-Ch?vez P, de Gortari P
Journal of neuroendocrinology29(11)(2017 Nov)

Glucose enhances rat islet function via stimulating CART expression.
Xu W, Zhang Y, Bai M, Zhou F, Deng R, Ji X, Zhang J, Liu Y, Zhou L, Wang X
Biochemical and biophysical research communications481(1-2)84-89(2016 Dec)

Levels of Cocaine- and Amphetamine-Regulated Transcript in Vagal Afferents in the Mouse Are Unaltered in Response to Metabolic Challenges.
Yuan X, Huang Y, Shah S, Wu H, Gautron L
eNeuro3(5)(2016 Sep-Oct)

Sequence variants in the human cocaine and amphetamine-regulated transcript (CART) gene in subjects with early onset obesity.
Echwald SM, S?rensen TI, Andersen T, Hansen C, Tommerup N, Pedersen O
Obesity research7(6)532-6(1999 Nov)

Purification and characterisation of a new hypothalamic satiety peptide, cocaine and amphetamine regulated transcript (CART), produced in yeast.
Thim L, Nielsen PF, Judge ME, Andersen AS, Diers I, Egel-Mitani M, Hastrup S
FEBS letters428(3)263-8(1998 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

CART is important for maintaining the beta-cell phenotype.
Title: Endogenous beta-cell CART regulates insulin secretion and transcription of beta-cell genes.

These findings suggest that islet-derived CART is involved, at least in part, in high glucose-potentiated pancreatic beta-cell function.
Title: Glucose enhances rat islet function via stimulating CART expression.

Results suggest that CART plays an important role in recognition memory and that these effects may be mediated by NMDA receptors-ERK signaling in the entorhinal/perirhinal -hippocampal circuit.
Title: Pro-cognitive action of CART is mediated via ERK in the hippocampus.

Study found that the distribution and intensity of CART staining in the nodose ganglion looked identical in fed and fasted rats when observed under blinded conditions and the density of CART-positive profiles across the ganglion was not significantly different in fed and fasted rats.
Title: Levels of Cocaine- and Amphetamine-Regulated Transcript in Vagal Afferents in the Mouse Are Unaltered in Response to Metabolic Challenges.

Cocaine- and amphetamine-regulated transcript (CART) mRNA and protein were both up-regulated in morphine-administered animals and also in the withdrawal group in the nucleus accumbens (NAc), striatum and prefrontal cortex (PFC). In the addicted group, CART mRNA and protein were both down-regulated in NAc and striatum.
Title: CART peptide and opioid addiction: Expression changes in male rat brain.

The following Cartpt gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cartpt cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa13514	NM_017110.1 Latest version!	Rattus norvegicus CART prepropeptide (Cartpt), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
ORa24347	XM_006231843.3 Latest version!	Rattus norvegicus CART prepropeptide (Cartpt), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cartpt ( NM_017110.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_058806.1 Rattus norvegicus CART prepropeptide (Cartpt), mRNA.

Price & Availability↑

CloneID	ORa13514
Clone ID Related Accession (Same CDS sequence)	NM_017110.1
Accession Version	NM_017110.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 390bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Rattus norvegicus(Norway rat)
Product	cocaine- and amphetamine-regulated transcript protein precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U10071.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U10071.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296 [ECO:0000348] ##Evidence-Data-END##

ATGGAGAGCT CCCGCCTGCG GCTGCTACCC GTCCTGGGCG CCGCCCTACT GCTGCTGCTA 
CCTTTGCTGG GTGCCGGTGC CCAGGAGGAT GCCGAGCTGC AGCCCCGAGC CCTGGACATC 
TACTCTGCCG TGGATGATGC GTCCCATGAG AAGGAGCTGC CAAGGCGGCA ACTTCGGGCT 
CCCGGCGCTG TGTTGCAGAT TGAAGCGCTG CAGGAAGTCC TGAAGAAGCT CAAGAGTAAA 
CGCATTCCGA TCTATGAGAA GAAGTACGGC CAAGTCCCCA TGTGTGACGC TGGAGAGCAG 
TGCGCAGTGC GGAAAGGGGC CAGGATCGGG AAGCTGTGTG ACTGTCCCCG AGGAACTTCT 
TGCAATTCTT TCCTCTTGAA GTGCTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_058806.1
CDS	20..409
Translation	MESSRLRLLPVLGAALLLLLPLLGAGAQEDAELQPRALDIYSAVDDASHEKELPRRQLRAPGAVLQIEALQEVLKKLKSKRIPIYEKKYGQVPMCDAGEQCAVRKGARIGKLCDCPRGTSCNSFLLKCL

Target ORF information:

RefSeq Version	NM_017110.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus CART prepropeptide (Cartpt), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017110.1

ORF Insert Sequence:

ATGGAGAGCT CCCGCCTGCG GCTGCTACCC GTCCTGGGCG CCGCCCTACT GCTGCTGCTA 
CCTTTGCTGG GTGCCGGTGC CCAGGAGGAT GCCGAGCTGC AGCCCCGAGC CCTGGACATC 
TACTCTGCCG TGGATGATGC GTCCCATGAG AAGGAGCTGC CAAGGCGGCA ACTTCGGGCT 
CCCGGCGCTG TGTTGCAGAT TGAAGCGCTG CAGGAAGTCC TGAAGAAGCT CAAGAGTAAA 
CGCATTCCGA TCTATGAGAA GAAGTACGGC CAAGTCCCCA TGTGTGACGC TGGAGAGCAG 
TGCGCAGTGC GGAAAGGGGC CAGGATCGGG AAGCTGTGTG ACTGTCCCCG AGGAACTTCT 
TGCAATTCTT TCCTCTTGAA GTGCTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cartpt ( XM_006231843.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006231905.1 Rattus norvegicus CART prepropeptide (Cartpt), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa24347
Clone ID Related Accession (Same CDS sequence)	XM_006231843.3
Accession Version	XM_006231843.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 351bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	cocaine- and amphetamine-regulated transcript protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006231843.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGCT CCCGCCTGCG GCTGCTACCC GTCCTGGGCG CCGCCCTACT GCTGCTGCTA 
CCTTTGCTGG GTGCCGGTGC CCAGGAGGAT GCCGAGCTGC AGCCCCGAGC CCTGGACATC 
TACTCTGCCG TGGATGATGC GTCCCATGAG AAGGAGCTGA TTGAAGCGCT GCAGGAAGTC 
CTGAAGAAGC TCAAGAGTAA ACGCATTCCG ATCTATGAGA AGAAGTACGG CCAAGTCCCC 
ATGTGTGACG CTGGAGAGCA GTGCGCAGTG CGGAAAGGGG CCAGGATCGG GAAGCTGTGT 
GACTGTCCCC GAGGAACTTC TTGCAATTCT TTCCTCTTGA AGTGCTTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006231905.1
CDS	159..509
Translation	MESSRLRLLPVLGAALLLLLPLLGAGAQEDAELQPRALDIYSAVDDASHEKELIEALQEVLKKLKSKRIPIYEKKYGQVPMCDAGEQCAVRKGARIGKLCDCPRGTSCNSFLLKCL

Target ORF information:

RefSeq Version	XM_006231843.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus CART prepropeptide (Cartpt), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006231843.3

ORF Insert Sequence:

ATGGAGAGCT CCCGCCTGCG GCTGCTACCC GTCCTGGGCG CCGCCCTACT GCTGCTGCTA 
CCTTTGCTGG GTGCCGGTGC CCAGGAGGAT GCCGAGCTGC AGCCCCGAGC CCTGGACATC 
TACTCTGCCG TGGATGATGC GTCCCATGAG AAGGAGCTGA TTGAAGCGCT GCAGGAAGTC 
CTGAAGAAGC TCAAGAGTAA ACGCATTCCG ATCTATGAGA AGAAGTACGG CCAAGTCCCC 
ATGTGTGACG CTGGAGAGCA GTGCGCAGTG CGGAAAGGGG CCAGGATCGG GAAGCTGTGT 
GACTGTCCCC GAGGAACTTC TTGCAATTCT TTCCTCTTGA AGTGCTTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Impaired hypothalamic cocaine- and amphetamine-regulated transcript expression in lateral hypothalamic area and paraventricular nuclei of dehydration-induced anorexic rats.
Journal of neuroendocrinology29(11)(2017 Nov)
Garc?a-Luna C,Soberanes-Ch?vez P,de Gortari P

Glucose enhances rat islet function via stimulating CART expression.
Biochemical and biophysical research communications481(1-2)84-89(2016 Dec)
Xu W,Zhang Y,Bai M,Zhou F,Deng R,Ji X,Zhang J,Liu Y,Zhou L,Wang X

Levels of Cocaine- and Amphetamine-Regulated Transcript in Vagal Afferents in the Mouse Are Unaltered in Response to Metabolic Challenges.
eNeuro3(5)(2016 Sep-Oct)
Yuan X,Huang Y,Shah S,Wu H,Gautron L

Sequence variants in the human cocaine and amphetamine-regulated transcript (CART) gene in subjects with early onset obesity.
Obesity research7(6)532-6(1999 Nov)
Echwald SM,S?rensen TI,Andersen T,Hansen C,Tommerup N,Pedersen O

Purification and characterisation of a new hypothalamic satiety peptide, cocaine and amphetamine regulated transcript (CART), produced in yeast.
FEBS letters428(3)263-8(1998 May)
Thim L,Nielsen PF,Judge ME,Andersen AS,Diers I,Egel-Mitani M,Hastrup S