Gcm2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gcm2
Entrez Gene ID	291047
Full Name	glial cells missing homolog 2
General protein information	Preferred Names glial cells missing homolog 2 Names chorion-specific transcription factor GCMb
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 17 Map Location: 17p13

mRNA and Protein(s)

mRNA	Protein	Name
NM_001106105.1	NP_001099575.1	chorion-specific transcription factor GCMb

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	gcm2	XP_002932714.1
	GCM2	XP_001088392.1
Rattus norvegicus (Norway rat)	Gcm2	NP_001099575.1
Danio rerio (zebrafish)	gcm2	NP_001005603.1
Homo sapiens (human)	GCM2	NP_004743.1
Bos taurus (cattle)	GCM2	XP_002697663.2
Mus musculus (house mouse)	Gcm2	NP_032130.2
Gallus gallus (chicken)	GCM2	NP_001008480.1
Pan troglodytes (chimpanzee)	GCM2	XP_518235.2
Canis lupus familiaris (dog)	GCM2	XP_545339.3

Gene Ontology
Bibliography

Related articles in PubMed

GCM2-Activating Mutations in Familial Isolated Hyperparathyroidism.
Guan B, Welch JM, Sapp JC, Ling H, Li Y, Johnston JJ, Kebebew E, Biesecker LG, Simonds WF, Marx SJ, Agarwal SK
American journal of human genetics99(5)1034-1044(2016 Nov)

Gata3-deficient mice develop parathyroid abnormalities due to dysregulation of the parathyroid-specific transcription factor Gcm2.
Grigorieva IV, Mirczuk S, Gaynor KU, Nesbit MA, Grigorieva EF, Wei Q, Ali A, Fairclough RJ, Stacey JM, Stechman MJ, Mihai R, Kurek D, Fraser WD, Hough T, Condie BG, Manley N, Grosveld F, Thakker RV
The Journal of clinical investigation120(6)2144-55(2010 Jun)

Identification and characterization of novel parathyroid-specific transcription factor Glial Cells Missing Homolog B (GCMB) mutations in eight families with autosomal recessive hypoparathyroidism.
Bowl MR, Mirczuk SM, Grigorieva IV, Piret SE, Cranston T, Southam L, Allgrove J, Bahl S, Brain C, Loughlin J, Mughal Z, Ryan F, Shaw N, Thakker YV, Tiosano D, Nesbit MA, Thakker RV
Human molecular genetics19(10)2028-38(2010 May)

Parathyroid hormone regulates fetal-placental mineral homeostasis.
Simmonds CS, Karsenty G, Karaplis AC, Kovacs CS
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research25(3)594-605(2010 Mar)

Gcm2 is required for the differentiation and survival of parathyroid precursor cells in the parathyroid/thymus primordia.
Liu Z, Yu S, Manley NR
Developmental biology305(1)333-46(2007 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Gcm2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gcm2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa04458	NM_001106105.1 Latest version!	Rattus norvegicus glial cells missing transcription factor 2 (Gcm2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gcm2 ( NM_001106105.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001099575.1 Rattus norvegicus glial cells missing transcription factor 2 (Gcm2), mRNA.

Price & Availability↑

CloneID	ORa04458
Clone ID Related Accession (Same CDS sequence)	NM_001106105.1
Accession Version	NM_001106105.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1515bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-18
Organism	Rattus norvegicus(Norway rat)
Product	chorion-specific transcription factor GCMb
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473977.1. On or before Oct 4, 2007 this sequence version replaced XM_225247.4, XM_001061843.1.

ATGCCAGCAG ACAGCATGCA GGACCCCGAT CCTGTGCTTT CCTACGGGAT GAAACTCACA 
TGGGACATCA ATGACCCGCA GATGCCTCAG GAACCAGCTC ACTTCGACCA CTTCCGGGAG 
TGGCCTGATG GCTACGTGCG CTTCATCTAC AGCAGTCAGG AGAAGAAAGC TCAGCGCCAC 
CTGAGCGGCT GGGCCATGCG CAACACCAAC AACCACAATG GCCACATCCT CAAAAAGTCC 
TGCCTGGGCG TGGTGGTGTG CGCACGCGCC TGCGCCCTGA AGGATGGCTC GCACCTGCAG 
CTGAGGCCAG CCATCTGCGA CAAGGCTCGT CTGAAGCAAC AAAAGAAAGC TTGCCCCAAC 
TGTCATTCAG CTTTGGAGCT GGTCCCTTGC CGAGGACACA GTGGATACCC TGTCACCAAC 
TTCTGGAGAC TTGATGGCAA TGCAATATTT TTTCAGGCCA AAGGAGTTCA TGATCATCCC 
AGACCAGAGA GCAAGTCAGA GACAGAAGGC AGAAGAAGTG CCCTCAAGAG ACAGATGGCC 
TCTTTCTACC AACCCCAGAA AAGGAGATCA GAGGAGCCTG AGGCAGGAAG CACTCAGGAC 
ACCAGGGGAC ACTCCAATAG CACGCCTGTC CCAGAACCCT CAGAACTGTT TGCTATGCCT 
GCTGACACCA GCTTCCCTAT TTCAGGGCAT TCTTCCCTTT CCTTCCCAAA CTCTGATGTC 
CACAGAGTTA CCTGTGACCT GCCCACCTTT CCAGGAGATG TAATACTTCC CTTTCAGAAA 
TATTCAAATC CAAGCATTTA TTTCCCTGGG CCACCTTGGA GCTATGAATT GGCAAGTCCT 
GGAGTTACGG GTTCAAGTCC ATATCCTACC CTGTATAAAG ACCCCTCCAT TGTCCCCGAT 
GACCCTGACT GGATCCATCT AAACTCACTA CAATATAATG TGAGTTCATA CGGCAGCTAT 
GAGAGAAGCT TGGATCTCAC AGCTAGATAT CATGGCTGGA AACCTACACA TGGGAAAGCT 
GGCCTTGAGG AGAAGGTTGA CTGTAGGCCA TGCCAGGCTG TGCCCACATC GCCTTATTAC 
AACCTAGAGC TGCCCTGCAG GTACCTGCCA GTGGCCCCGG CAGGTACCCA GGCCCTGCAG 
ACAGTGATCA CCACCACAGT GGCTTATCAG GCTTACCAGC ACCCTGCTCT GAAACACGGC 
GACAGCGTGC AGGAGGTTAG CAGCCTTGCC AGTTGCACCT ACGCTTCTGA AAACCTCCCA 
ATGCCCATCT ATCCGCAAGC CTTAGACCCT CAAGAGGGAG CCATCCAGGC AGCCTCCCCT 
TCAGGGAGAG CCCCTGGGAT AGTCCCTGGA GATTGCCAGG CAGCCAGACC TACTCTGGCT 
TTCCCTCAAG AAACAGATCC CTCCAGGACA GCTGGAGCAG ATGTGTGGGA TGTGTGTCTG 
TCTGGGGTGG GCTCTGTGAT GGCTTACTTG GATAGGACAG GGCAACCCTT TAACCTCGAC 
AATGAGGACT TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001099575.1
CDS	181..1695
Translation	MPADSMQDPDPVLSYGMKLTWDINDPQMPQEPAHFDHFREWPDGYVRFIYSSQEKKAQRHLSGWAMRNTNNHNGHILKKSCLGVVVCARACALKDGSHLQLRPAICDKARLKQQKKACPNCHSALELVPCRGHSGYPVTNFWRLDGNAIFFQAKGVHDHPRPESKSETEGRRSALKRQMASFYQPQKRRSEEPEAGSTQDTRGHSNSTPVPEPSELFAMPADTSFPISGHSSLSFPNSDVHRVTCDLPTFPGDVILPFQKYSNPSIYFPGPPWSYELASPGVTGSSPYPTLYKDPSIVPDDPDWIHLNSLQYNVSSYGSYERSLDLTARYHGWKPTHGKAGLEEKVDCRPCQAVPTSPYYNLELPCRYLPVAPAGTQALQTVITTTVAYQAYQHPALKHGDSVQEVSSLASCTYASENLPMPIYPQALDPQEGAIQAASPSGRAPGIVPGDCQAARPTLAFPQETDPSRTAGADVWDVCLSGVGSVMAYLDRTGQPFNLDNEDF

Target ORF information:

RefSeq Version	NM_001106105.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus glial cells missing transcription factor 2 (Gcm2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001106105.1

ORF Insert Sequence:

ATGCCAGCAG ACAGCATGCA GGACCCCGAT CCTGTGCTTT CCTACGGGAT GAAACTCACA 
TGGGACATCA ATGACCCGCA GATGCCTCAG GAACCAGCTC ACTTCGACCA CTTCCGGGAG 
TGGCCTGATG GCTACGTGCG CTTCATCTAC AGCAGTCAGG AGAAGAAAGC TCAGCGCCAC 
CTGAGCGGCT GGGCCATGCG CAACACCAAC AACCACAATG GCCACATCCT CAAAAAGTCC 
TGCCTGGGCG TGGTGGTGTG CGCACGCGCC TGCGCCCTGA AGGATGGCTC GCACCTGCAG 
CTGAGGCCAG CCATCTGCGA CAAGGCTCGT CTGAAGCAAC AAAAGAAAGC TTGCCCCAAC 
TGTCATTCAG CTTTGGAGCT GGTCCCTTGC CGAGGACACA GTGGATACCC TGTCACCAAC 
TTCTGGAGAC TTGATGGCAA TGCAATATTT TTTCAGGCCA AAGGAGTTCA TGATCATCCC 
AGACCAGAGA GCAAGTCAGA GACAGAAGGC AGAAGAAGTG CCCTCAAGAG ACAGATGGCC 
TCTTTCTACC AACCCCAGAA AAGGAGATCA GAGGAGCCTG AGGCAGGAAG CACTCAGGAC 
ACCAGGGGAC ACTCCAATAG CACGCCTGTC CCAGAACCCT CAGAACTGTT TGCTATGCCT 
GCTGACACCA GCTTCCCTAT TTCAGGGCAT TCTTCCCTTT CCTTCCCAAA CTCTGATGTC 
CACAGAGTTA CCTGTGACCT GCCCACCTTT CCAGGAGATG TAATACTTCC CTTTCAGAAA 
TATTCAAATC CAAGCATTTA TTTCCCTGGG CCACCTTGGA GCTATGAATT GGCAAGTCCT 
GGAGTTACGG GTTCAAGTCC ATATCCTACC CTGTATAAAG ACCCCTCCAT TGTCCCCGAT 
GACCCTGACT GGATCCATCT AAACTCACTA CAATATAATG TGAGTTCATA CGGCAGCTAT 
GAGAGAAGCT TGGATCTCAC AGCTAGATAT CATGGCTGGA AACCTACACA TGGGAAAGCT 
GGCCTTGAGG AGAAGGTTGA CTGTAGGCCA TGCCAGGCTG TGCCCACATC GCCTTATTAC 
AACCTAGAGC TGCCCTGCAG GTACCTGCCA GTGGCCCCGG CAGGTACCCA GGCCCTGCAG 
ACAGTGATCA CCACCACAGT GGCTTATCAG GCTTACCAGC ACCCTGCTCT GAAACACGGC 
GACAGCGTGC AGGAGGTTAG CAGCCTTGCC AGTTGCACCT ACGCTTCTGA AAACCTCCCA 
ATGCCCATCT ATCCGCAAGC CTTAGACCCT CAAGAGGGAG CCATCCAGGC AGCCTCCCCT 
TCAGGGAGAG CCCCTGGGAT AGTCCCTGGA GATTGCCAGG CAGCCAGACC TACTCTGGCT 
TTCCCTCAAG AAACAGATCC CTCCAGGACA GCTGGAGCAG ATGTGTGGGA TGTGTGTCTG 
TCTGGGGTGG GCTCTGTGAT GGCTTACTTG GATAGGACAG GGCAACCCTT TAACCTCGAC 
AATGAGGACT TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

GCM2-Activating Mutations in Familial Isolated Hyperparathyroidism.
American journal of human genetics99(5)1034-1044(2016 Nov)
Guan B,Welch JM,Sapp JC,Ling H,Li Y,Johnston JJ,Kebebew E,Biesecker LG,Simonds WF,Marx SJ,Agarwal SK

Gata3-deficient mice develop parathyroid abnormalities due to dysregulation of the parathyroid-specific transcription factor Gcm2.
The Journal of clinical investigation120(6)2144-55(2010 Jun)
Grigorieva IV,Mirczuk S,Gaynor KU,Nesbit MA,Grigorieva EF,Wei Q,Ali A,Fairclough RJ,Stacey JM,Stechman MJ,Mihai R,Kurek D,Fraser WD,Hough T,Condie BG,Manley N,Grosveld F,Thakker RV

Identification and characterization of novel parathyroid-specific transcription factor Glial Cells Missing Homolog B (GCMB) mutations in eight families with autosomal recessive hypoparathyroidism.
Human molecular genetics19(10)2028-38(2010 May)
Bowl MR,Mirczuk SM,Grigorieva IV,Piret SE,Cranston T,Southam L,Allgrove J,Bahl S,Brain C,Loughlin J,Mughal Z,Ryan F,Shaw N,Thakker YV,Tiosano D,Nesbit MA,Thakker RV

Parathyroid hormone regulates fetal-placental mineral homeostasis.
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research25(3)594-605(2010 Mar)
Simmonds CS,Karsenty G,Karaplis AC,Kovacs CS

Gcm2 is required for the differentiation and survival of parathyroid precursor cells in the parathyroid/thymus primordia.
Developmental biology305(1)333-46(2007 May)
Liu Z,Yu S,Manley NR