Id4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Id4
Entrez Gene ID	291023
Full Name	inhibitor of DNA binding 4, HLH protein
Synonyms	Idb4
General protein information	Preferred Names inhibitor of DNA binding 4, HLH protein Names DNA-binding protein inhibitor ID-4 inhibitor of DNA binding 4, dominant negative helix-loop-helix protein
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 17 Map Location: 17p14
Summary	involved in the regulation of basic HLH transcription factors; may play a role in mammary gland carcinogenesis [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_175582.1	NP_783172.1	DNA-binding protein inhibitor ID-4

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Id4	NP_112443.1
Xenopus tropicalis (tropical clawed frog)	id4	NP_001004839.1
Rattus norvegicus (Norway rat)	Id4	NP_783172.1
	ID4	XP_001098022.1
Danio rerio (zebrafish)	id4	NP_001035079.1
Homo sapiens (human)	ID4	NP_001537.1

Gene Ontology
Bibliography

Related articles in PubMed

Astrocytes induce proliferation of oligodendrocyte progenitor cells via connexin 47-mediated activation of the ERK/Id4 pathway.
Liu Z, Xu D, Wang S, Chen Y, Li Z, Gao X, Jiang L, Tang Y, Peng Y
Cell cycle (Georgetown, Tex.)16(7)714-722(2017 Apr)

Id4 deficiency attenuates prostate development and promotes PIN-like lesions by regulating androgen receptor activity and expression of NKX3.1 and PTEN.
Sharma P, Knowell AE, Chinaranagari S, Komaragiri S, Nagappan P, Patel D, Havrda MC, Chaudhary J
Molecular cancer1267(2013 Jun)

Type II arginine methyltransferase PRMT5 regulates gene expression of inhibitors of differentiation/DNA binding Id2 and Id4 during glial cell differentiation.
Huang J, Vogel G, Yu Z, Almazan G, Richard S
The Journal of biological chemistry286(52)44424-32(2011 Dec)

Olig1 and ID4 interactions in living cells visualized by bimolecular fluorescence complementation technique.
Guo SJ, Hu JG, Zhao BM, Shen L, Wang R, Zhou JS, L? HZ
Molecular biology reports38(7)4637-42(2011 Oct)

Inhibitor of DNA binding 4 is expressed selectively by single spermatogonia in the male germline and regulates the self-renewal of spermatogonial stem cells in mice.
Oatley MJ, Kaucher AV, Racicot KE, Oatley JM
Biology of reproduction85(2)347-56(2011 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Astrocytes can cause Ca(2+) signaling activation, ERK1/2 phosphorylation, and Id4 expression stimulation in oligodendrocyte progenitor cells inducing proliferation of these cells, mainly through Cx47.
Title: Astrocytes induce proliferation of oligodendrocyte progenitor cells via connexin 47-mediated activation of the ERK/Id4 pathway.

CpG-rich islands within the Id2 and Id4 genes were bound by PRMT5 and were hypomethylated in PRMT5-deficient cells, suggesting that PRMT5 plays a role in gene silencing during glial cell
Title: Type II arginine methyltransferase PRMT5 regulates gene expression of inhibitors of differentiation/DNA binding Id2 and Id4 during glial cell differentiation.

Bimolecular fluorescence complementation (BiFC) analysis was performed to further characterize the distinct subcellular localization of olig1, ID4 and their dimer.
Title: Olig1 and ID4 interactions in living cells visualized by bimolecular fluorescence complementation technique.

These results indicate that ID4 is a distinguishing marker of spermatogonial stem cells in the mammalian germline and plays an important role in the regulation of self-renewal.
Title: Inhibitor of DNA binding 4 is expressed selectively by single spermatogonia in the male germline and regulates the self-renewal of spermatogonial stem cells in mice.

Id2, Id3, and Id4 proteins have roles in vibrissae follicle development
Title: Id2, Id3, and Id4 proteins show dynamic changes in expression during vibrissae follicle development.

The following Id4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Id4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa03798	NM_175582.1 Latest version!	Rattus norvegicus inhibitor of DNA binding 4, HLH protein (Id4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Id4 ( NM_175582.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_783172.1 Rattus norvegicus inhibitor of DNA binding 4, HLH protein (Id4), mRNA.

Price & Availability↑

CloneID	ORa03798
Clone ID Related Accession (Same CDS sequence)	NM_175582.1
Accession Version	NM_175582.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 486bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-26
Organism	Rattus norvegicus(Norway rat)
Product	DNA-binding protein inhibitor ID-4
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF468681.1. On Dec 17, 2003 this sequence version replaced XM_214444.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGAAGGCGG TGAGCCCGGT GCGCCCCTCT GGCCGCAAGG CGCCGTCGGG CTGCGGCGGC 
GGGGAGCTGG CGCTACGTTG CCTGGCGGAG CATGGCCATA GCCTAGGTGG CTCGGCAGCC 
GCGGCGGCGG CGGCGGCGGC AGCGCGCTGC AAGGCGGCCG AGGCGGCGGC CGACGAGCCG 
GCACTGTGCC TGCAGTGCGA TATGAACGAC TGCTACACTC GCCTGCGGAG GCTCGTGCCC 
ACCATCCCGC CCAACAAGAA AGTCAGCAAA GTGGAGATCC TGCAGCACGT TATCGACTAC 
ATCCTGGACC TGCAGCTGGC GCTGGAGACG CACCCTGCTC TGCTGAGACA GCCGCCACCG 
CCCGCGCCAC CGCTCCACTC GGCCGGGGCT TGCCCGGTCG CGTCGCAGCG GACCCCGCTT 
ACGGCGCTCA ACACTGACCC GGCCGGCGCC GTGAACAAGC AGGGCGACAG CATTCTCTGC 
CGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_783172.1
CDS	1..486
Translation	MKAVSPVRPSGRKAPSGCGGGELALRCLAEHGHSLGGSAAAAAAAAAARCKAAEAAADEPALCLQCDMNDCYTRLRRLVPTIPPNKKVSKVEILQHVIDYILDLQLALETHPALLRQPPPPAPPLHSAGACPVASQRTPLTALNTDPAGAVNKQGDSILCR

Target ORF information:

RefSeq Version	NM_175582.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus inhibitor of DNA binding 4, HLH protein (Id4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_175582.1

ORF Insert Sequence:

ATGAAGGCGG TGAGCCCGGT GCGCCCCTCT GGCCGCAAGG CGCCGTCGGG CTGCGGCGGC 
GGGGAGCTGG CGCTACGTTG CCTGGCGGAG CATGGCCATA GCCTAGGTGG CTCGGCAGCC 
GCGGCGGCGG CGGCGGCGGC AGCGCGCTGC AAGGCGGCCG AGGCGGCGGC CGACGAGCCG 
GCACTGTGCC TGCAGTGCGA TATGAACGAC TGCTACACTC GCCTGCGGAG GCTCGTGCCC 
ACCATCCCGC CCAACAAGAA AGTCAGCAAA GTGGAGATCC TGCAGCACGT TATCGACTAC 
ATCCTGGACC TGCAGCTGGC GCTGGAGACG CACCCTGCTC TGCTGAGACA GCCGCCACCG 
CCCGCGCCAC CGCTCCACTC GGCCGGGGCT TGCCCGGTCG CGTCGCAGCG GACCCCGCTT 
ACGGCGCTCA ACACTGACCC GGCCGGCGCC GTGAACAAGC AGGGCGACAG CATTCTCTGC 
CGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Astrocytes induce proliferation of oligodendrocyte progenitor cells via connexin 47-mediated activation of the ERK/Id4 pathway.
Cell cycle (Georgetown, Tex.)16(7)714-722(2017 Apr)
Liu Z,Xu D,Wang S,Chen Y,Li Z,Gao X,Jiang L,Tang Y,Peng Y

Id4 deficiency attenuates prostate development and promotes PIN-like lesions by regulating androgen receptor activity and expression of NKX3.1 and PTEN.
Molecular cancer1267(2013 Jun)
Sharma P,Knowell AE,Chinaranagari S,Komaragiri S,Nagappan P,Patel D,Havrda MC,Chaudhary J

Type II arginine methyltransferase PRMT5 regulates gene expression of inhibitors of differentiation/DNA binding Id2 and Id4 during glial cell differentiation.
The Journal of biological chemistry286(52)44424-32(2011 Dec)
Huang J,Vogel G,Yu Z,Almazan G,Richard S

Olig1 and ID4 interactions in living cells visualized by bimolecular fluorescence complementation technique.
Molecular biology reports38(7)4637-42(2011 Oct)
Guo SJ,Hu JG,Zhao BM,Shen L,Wang R,Zhou JS,L? HZ

Inhibitor of DNA binding 4 is expressed selectively by single spermatogonia in the male germline and regulates the self-renewal of spermatogonial stem cells in mice.
Biology of reproduction85(2)347-56(2011 Aug)
Oatley MJ,Kaucher AV,Racicot KE,Oatley JM