Usp4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Usp4
Entrez Gene ID	290864
Full Name	ubiquitin specific peptidase 4
General protein information	Preferred Names ubiquitin specific peptidase 4 Names ubiquitin carboxyl-terminal hydrolase 4 deubiquitinating enzyme 4 ubiquitin specific peptidase 4 (proto-oncogene) ubiquitin specific protease 4 (proto-oncogene) ubiquitin thioesterase 4 ubiquitin-specific-processing protease 4
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 8 Map Location: 8q32

mRNA and Protein(s)

mRNA	Protein	Name
NM_001106093.2	NP_001099563.1	ubiquitin carboxyl-terminal hydrolase 4 isoform b
NM_001135012.1	NP_001128484.1	ubiquitin carboxyl-terminal hydrolase 4 isoform a

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	USP4	XP_003432923.1
Gallus gallus (chicken)	USP4	NP_001006134.1
Homo sapiens (human)	USP4	NP_003354.2
	USP4	XP_002802844.1
Bos taurus (cattle)	USP4	NP_001093789.1
Rattus norvegicus (Norway rat)	Usp4	NP_001128484.1
Xenopus tropicalis (tropical clawed frog)	usp4	XP_002936515.1
Pan troglodytes (chimpanzee)	USP4	XP_001163445.1
Danio rerio (zebrafish)	usp4	XP_002662556.2
Mus musculus (house mouse)	Usp4	NP_035808.2
Caenorhabditis elegans (roundworm)	H34C03.2	NP_501035.1
Arabidopsis thaliana (thale cress)	UBP5	NP_565944.1

Gene Ontology
Bibliography

Related articles in PubMed

Downregulation of USP4 Promotes Activation of Microglia and Subsequent Neuronal Inflammation in Rat Spinal Cord After Injury.
Jiang X, Yu M, Ou Y, Cao Y, Yao Y, Cai P, Zhang F
Neurochemical research42(11)3245-3253(2017 Nov)

Increased Expression of Ubiquitin-Specific Protease 4 Participates in Neuronal Apoptosis After Intracerebral Hemorrhage in Adult Rats.
Liu C, Liu C, Liu H, Gong L, Tao T, Shen Y, Zhu S, Shen A
Cellular and molecular neurobiology37(3)427-435(2017 Apr)

Structural variability of the ubiquitin specific protease DUSP-UBL double domains.
Elliott PR, Liu H, Pastok MW, Grossmann GJ, Rigden DJ, Clague MJ, Urb? S, Barsukov IL
FEBS letters585(21)3385-90(2011 Nov)

The Prp19 complex and the Usp4Sart3 deubiquitinating enzyme control reversible ubiquitination at the spliceosome.
Song EJ, Werner SL, Neubauer J, Stegmeier F, Aspden J, Rio D, Harper JW, Elledge SJ, Kirschner MW, Rape M
Genes & development24(13)1434-47(2010 Jul)

UnpEL/Usp4 is ubiquitinated by Ro52 and deubiquitinated by itself.
Wada K, Kamitani T
Biochemical and biophysical research communications342(1)253-8(2006 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

USP4 may be involved in neuronal apoptosis after intracerebral hemorrhage.
Title: Increased Expression of Ubiquitin-Specific Protease 4 Participates in Neuronal Apoptosis After Intracerebral Hemorrhage in Adult Rats.

The following Usp4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Usp4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa00165	NM_001106093.2 Latest version!	Rattus norvegicus ubiquitin specific peptidase 4 (Usp4), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$538.30 $769.00
ORa03859	NM_001135012.1 Latest version!	Rattus norvegicus ubiquitin specific peptidase 4 (Usp4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Usp4 ( NM_001106093.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001099563.1 Rattus norvegicus ubiquitin specific peptidase 4 (Usp4), transcript variant 2, mRNA.

Price & Availability↑

CloneID	ORa00165
Clone ID Related Accession (Same CDS sequence)	NM_001106093.2
Accession Version	NM_001106093.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2745bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-30
Organism	Rattus norvegicus(Norway rat)
Product	ubiquitin carboxyl-terminal hydrolase 4 isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC128721.4. On Sep 16, 2008 this sequence version replaced NM_001106093.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052297, SAMEA2689594 [ECO:0000350] ##Evidence-Data-END##

ATGGCGGAAG GCCGGGGCAC CCATGAGCGA CCGGATGTGG AGACCCAGAA GACGGAGCTC 
GGAGCCTTGA TGGGGACCAC GCTCCAGCGT GGGGCTCAGT GGTATCTGAT CGACAGCCGG 
TGGTTCAAGC AGTGGAAGAA GTACGTCGGC TTTGACAGCT GGGACATGTA CAACGTGGGG 
GAGCACAATC TCTTTCCTGG ACCGATTGAC AACTCTGGGC TCTTCTCAGA TCCTGAGAGT 
CAGACCTTGA AGGAACACTT AATCGATGAG CTGGACTATG TGTTGGTTCC AACTGAAGCC 
TGGAATAAAT TGCTAAATTG GTATGGCTGT GTGGAAGGCC AGCAGCCTAT TGTCAGAAAA 
GTTGTGGAGC ATGGCCTGTT TGTCAAGCAC TGCAAAGTGG AAGTGTATTT GCTGGAGTTG 
AAGCTCTGTG AGAACAGTGA CCCCACCAAT GTGCTAAGTT GCCATTTTAG CAAAGCAGAT 
ACCATTGCAA CTATTGAGAA GGAGATGAGG AAGCTCTTCA ACATCCCTGC AGAGCGTGAA 
ACACGACTTT GGAACAAATA CATGAGCAAC ACCTATGAGC AGTTGAGCAA GCTAGACAAC 
ACTATCCAGG ATGCTGGGCT GTACCAGGGT CAGGTGCTAG TAATTGAGCC CCAAAATGAA 
GACGGCACAT GGCCCAGGCA GACCCTGCAA TCAAATGGAG CTGGCTTCTC TGCCTCGTAT 
AATTGCCAGG AGCCCCCATC ACCTCATATC CAGCCTGGCC TCTGTGGACT CGGAAACCTG 
GGAAACACCT GTTTTATGAA TTCTGCTTTG CAGTGCCTGA GCAACACTGG TCCACTGACT 
GAGTACTTTC TCAAAGATGA GTATGAGGCC GAGATCAACA GAGACAACCC TCTGGGGATG 
AAAGGGGAGA TTGCAGAGGC CTATGCAGAG CTCATCAAGC AGATGTGGTC TGGAAGGGAC 
ACCCACGTAG CACCACGGAT GTTCAAAACG CAAGTGGGAC GTTTCGCCCC TCAGTTTTCT 
GGCTACCAAC AACAAGACTC TCAAGAGCTG TTAGCCTTTA TTCTAGATGG ATTGCATGAA 
GACCTGAACC GAGTAAAGAA GAAGCCTTAC CTGGAGCCAA AGGATGCCAA TGGGCGACCA 
GATGCGGTGG TAGCAAAGGA AGCCTGGGAA AATCACAGGC TGAGGAATGA CTCTGTGATT 
GTGGATACTT TCCATGGCCT TTTCAAATCT ACTTTGGTTT GCCCAGAATG TGCTAAAGTT 
TCTGTGACCT TTGACCCATT TTGCTATCTA ACTCTCCCAC TGCCTTTGAA GAAAGATCGA 
ATTATGGAGG TCTTCCTGGT TCCTGCTGAC CCTCATTGCA GACCTATCCA GTACCGTGTG 
ACTGTGCCAT TGATGGGGGC CATTTCTGAC CTGTGTGAAG CACTCTCCAA GCTGTCTGGC 
ATTGCTGCAG AAAATATGGT GGTCACTGAT GTGTATAATC ACCGCTTCCA CAAAATTTTC 
CAAATGGATG AAGGTTTAAG CCACATCACG CCTCGAGATG ACATTTTTGT GTATGAGATC 
TGCACCACTC CCATGGATGG CTCAGAATAT ATCACTCTCC CAGTCTACTT CAGAGAAAAG 
AAGTCCAGGC CATCGAGTAC TTCCTCTGGG GCCGTGCTCT ATGGACAGCC ACTTCTTGTT 
TCTGTCCCTA AGCACAGACT AACCCTCGAG TCCTTATACC AGGCTGTTTG TGAACGTATC 
AGCCGCTATA TAAAGCAGCC TTTGCCTGAG GAGTTTCTCA GCTCTCCCTT AGAGCCTGGG 
GCCTGCAATG GCTCTAGGGG TAGCTATGAA GGAGATGAAG AAGAAATGGA TCATCAAGAG 
GAAGGGAAAG AACAGCTTTC TGAAGTGGAA GAAAGTGGTG AGGACAGTCA GGGAGGGGAC 
CCTACTGAGA CCACCCAAAA GGCGAAAGGC CCGCCCCGCC ACAAGAGGCT TTTCACCTTC 
AGCCTCGTGA ACTCCTGTGG AACTGCTGAT ATCAACTCGC TGGCCACTGA CGGAAAACTC 
CTCAAGCTCA ACTCTCGATC CACACTGGCC ATTGACTGGG ACAGTGAGAC CCGAAGCCTT 
TACTTTGATG AGCAAGAATC TGAGGCCTGT GAGAAGCACA CGAGCATGTC CCAGCCTCAG 
AAGAAGAAGA AGGCTGCAAT AGCCCTGCGG GAGTGCATCG AGCTCTTTAC TACCATGGAG 
ACCCTTGGGG AGCATGACCC CTGGTACTGT CCCACCTGTA AGAAGCACCA ACAGGCAACA 
AAAAAGTTTG ATTTGTGGTC TTTGCCCAAG ATCCTGGTGG TTCATCTCAA ACGTTTCTCC 
TATAACAGAT ACTGGCGGGA TAAACTGGAC ACAGTTGTGG AATTCCCAGT AAGAGCTCTG 
AACATGTCCG AGTTTGTGTG TGACCGGGCA GCAAGGCCTT ATGTCTATGA CCTGATTGCT 
GTGTCCAATC ACTATGGCGC TATGGGGGTC GGTCACTATA CTGCATATGC GAAGAACAGA 
CTGAATGGGA AATGGTATTA CTTTGATGAT AGCAGCGTGT CCCTGGCCTC TGAGGATCAG 
ATAGTGACGA AGGCCGCCTA CGTGTTGTTT TACCAGCGTC GGGATGATGA GTGTCCCAGC 
ACCTCCTCAC CTGTCAGCTT CCCGGGTTCT GATGGAGGGG CGAAGCTCAG CAGCTCACAG 
CAGGACTTGG GGGAAGAGGA GGCTTACACC ATGGACACCA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001099563.1
CDS	25..2769
Translation	MAEGRGTHERPDVETQKTELGALMGTTLQRGAQWYLIDSRWFKQWKKYVGFDSWDMYNVGEHNLFPGPIDNSGLFSDPESQTLKEHLIDELDYVLVPTEAWNKLLNWYGCVEGQQPIVRKVVEHGLFVKHCKVEVYLLELKLCENSDPTNVLSCHFSKADTIATIEKEMRKLFNIPAERETRLWNKYMSNTYEQLSKLDNTIQDAGLYQGQVLVIEPQNEDGTWPRQTLQSNGAGFSASYNCQEPPSPHIQPGLCGLGNLGNTCFMNSALQCLSNTGPLTEYFLKDEYEAEINRDNPLGMKGEIAEAYAELIKQMWSGRDTHVAPRMFKTQVGRFAPQFSGYQQQDSQELLAFILDGLHEDLNRVKKKPYLEPKDANGRPDAVVAKEAWENHRLRNDSVIVDTFHGLFKSTLVCPECAKVSVTFDPFCYLTLPLPLKKDRIMEVFLVPADPHCRPIQYRVTVPLMGAISDLCEALSKLSGIAAENMVVTDVYNHRFHKIFQMDEGLSHITPRDDIFVYEICTTPMDGSEYITLPVYFREKKSRPSSTSSGAVLYGQPLLVSVPKHRLTLESLYQAVCERISRYIKQPLPEEFLSSPLEPGACNGSRGSYEGDEEEMDHQEEGKEQLSEVEESGEDSQGGDPTETTQKAKGPPRHKRLFTFSLVNSCGTADINSLATDGKLLKLNSRSTLAIDWDSETRSLYFDEQESEACEKHTSMSQPQKKKKAAIALRECIELFTTMETLGEHDPWYCPTCKKHQQATKKFDLWSLPKILVVHLKRFSYNRYWRDKLDTVVEFPVRALNMSEFVCDRAARPYVYDLIAVSNHYGAMGVGHYTAYAKNRLNGKWYYFDDSSVSLASEDQIVTKAAYVLFYQRRDDECPSTSSPVSFPGSDGGAKLSSSQQDLGEEEAYTMDTN

Target ORF information:

RefSeq Version	NM_001106093.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ubiquitin specific peptidase 4 (Usp4), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001106093.2

ORF Insert Sequence:

ATGGCGGAAG GCCGGGGCAC CCATGAGCGA CCGGATGTGG AGACCCAGAA GACGGAGCTC 
GGAGCCTTGA TGGGGACCAC GCTCCAGCGT GGGGCTCAGT GGTATCTGAT CGACAGCCGG 
TGGTTCAAGC AGTGGAAGAA GTACGTCGGC TTTGACAGCT GGGACATGTA CAACGTGGGG 
GAGCACAATC TCTTTCCTGG ACCGATTGAC AACTCTGGGC TCTTCTCAGA TCCTGAGAGT 
CAGACCTTGA AGGAACACTT AATCGATGAG CTGGACTATG TGTTGGTTCC AACTGAAGCC 
TGGAATAAAT TGCTAAATTG GTATGGCTGT GTGGAAGGCC AGCAGCCTAT TGTCAGAAAA 
GTTGTGGAGC ATGGCCTGTT TGTCAAGCAC TGCAAAGTGG AAGTGTATTT GCTGGAGTTG 
AAGCTCTGTG AGAACAGTGA CCCCACCAAT GTGCTAAGTT GCCATTTTAG CAAAGCAGAT 
ACCATTGCAA CTATTGAGAA GGAGATGAGG AAGCTCTTCA ACATCCCTGC AGAGCGTGAA 
ACACGACTTT GGAACAAATA CATGAGCAAC ACCTATGAGC AGTTGAGCAA GCTAGACAAC 
ACTATCCAGG ATGCTGGGCT GTACCAGGGT CAGGTGCTAG TAATTGAGCC CCAAAATGAA 
GACGGCACAT GGCCCAGGCA GACCCTGCAA TCAAATGGAG CTGGCTTCTC TGCCTCGTAT 
AATTGCCAGG AGCCCCCATC ACCTCATATC CAGCCTGGCC TCTGTGGACT CGGAAACCTG 
GGAAACACCT GTTTTATGAA TTCTGCTTTG CAGTGCCTGA GCAACACTGG TCCACTGACT 
GAGTACTTTC TCAAAGATGA GTATGAGGCC GAGATCAACA GAGACAACCC TCTGGGGATG 
AAAGGGGAGA TTGCAGAGGC CTATGCAGAG CTCATCAAGC AGATGTGGTC TGGAAGGGAC 
ACCCACGTAG CACCACGGAT GTTCAAAACG CAAGTGGGAC GTTTCGCCCC TCAGTTTTCT 
GGCTACCAAC AACAAGACTC TCAAGAGCTG TTAGCCTTTA TTCTAGATGG ATTGCATGAA 
GACCTGAACC GAGTAAAGAA GAAGCCTTAC CTGGAGCCAA AGGATGCCAA TGGGCGACCA 
GATGCGGTGG TAGCAAAGGA AGCCTGGGAA AATCACAGGC TGAGGAATGA CTCTGTGATT 
GTGGATACTT TCCATGGCCT TTTCAAATCT ACTTTGGTTT GCCCAGAATG TGCTAAAGTT 
TCTGTGACCT TTGACCCATT TTGCTATCTA ACTCTCCCAC TGCCTTTGAA GAAAGATCGA 
ATTATGGAGG TCTTCCTGGT TCCTGCTGAC CCTCATTGCA GACCTATCCA GTACCGTGTG 
ACTGTGCCAT TGATGGGGGC CATTTCTGAC CTGTGTGAAG CACTCTCCAA GCTGTCTGGC 
ATTGCTGCAG AAAATATGGT GGTCACTGAT GTGTATAATC ACCGCTTCCA CAAAATTTTC 
CAAATGGATG AAGGTTTAAG CCACATCACG CCTCGAGATG ACATTTTTGT GTATGAGATC 
TGCACCACTC CCATGGATGG CTCAGAATAT ATCACTCTCC CAGTCTACTT CAGAGAAAAG 
AAGTCCAGGC CATCGAGTAC TTCCTCTGGG GCCGTGCTCT ATGGACAGCC ACTTCTTGTT 
TCTGTCCCTA AGCACAGACT AACCCTCGAG TCCTTATACC AGGCTGTTTG TGAACGTATC 
AGCCGCTATA TAAAGCAGCC TTTGCCTGAG GAGTTTCTCA GCTCTCCCTT AGAGCCTGGG 
GCCTGCAATG GCTCTAGGGG TAGCTATGAA GGAGATGAAG AAGAAATGGA TCATCAAGAG 
GAAGGGAAAG AACAGCTTTC TGAAGTGGAA GAAAGTGGTG AGGACAGTCA GGGAGGGGAC 
CCTACTGAGA CCACCCAAAA GGCGAAAGGC CCGCCCCGCC ACAAGAGGCT TTTCACCTTC 
AGCCTCGTGA ACTCCTGTGG AACTGCTGAT ATCAACTCGC TGGCCACTGA CGGAAAACTC 
CTCAAGCTCA ACTCTCGATC CACACTGGCC ATTGACTGGG ACAGTGAGAC CCGAAGCCTT 
TACTTTGATG AGCAAGAATC TGAGGCCTGT GAGAAGCACA CGAGCATGTC CCAGCCTCAG 
AAGAAGAAGA AGGCTGCAAT AGCCCTGCGG GAGTGCATCG AGCTCTTTAC TACCATGGAG 
ACCCTTGGGG AGCATGACCC CTGGTACTGT CCCACCTGTA AGAAGCACCA ACAGGCAACA 
AAAAAGTTTG ATTTGTGGTC TTTGCCCAAG ATCCTGGTGG TTCATCTCAA ACGTTTCTCC 
TATAACAGAT ACTGGCGGGA TAAACTGGAC ACAGTTGTGG AATTCCCAGT AAGAGCTCTG 
AACATGTCCG AGTTTGTGTG TGACCGGGCA GCAAGGCCTT ATGTCTATGA CCTGATTGCT 
GTGTCCAATC ACTATGGCGC TATGGGGGTC GGTCACTATA CTGCATATGC GAAGAACAGA 
CTGAATGGGA AATGGTATTA CTTTGATGAT AGCAGCGTGT CCCTGGCCTC TGAGGATCAG 
ATAGTGACGA AGGCCGCCTA CGTGTTGTTT TACCAGCGTC GGGATGATGA GTGTCCCAGC 
ACCTCCTCAC CTGTCAGCTT CCCGGGTTCT GATGGAGGGG CGAAGCTCAG CAGCTCACAG 
CAGGACTTGG GGGAAGAGGA GGCTTACACC ATGGACACCA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Usp4 ( NM_001135012.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001128484.1 Rattus norvegicus ubiquitin specific peptidase 4 (Usp4), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ORa03859
Clone ID Related Accession (Same CDS sequence)	NM_001135012.1
Accession Version	NM_001135012.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2886bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-19
Organism	Rattus norvegicus(Norway rat)
Product	ubiquitin carboxyl-terminal hydrolase 4 isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC128721.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## CDS exon combination :: BC166460.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGCGGAAG GCCGGGGCAC CCATGAGCGA CCGGATGTGG AGACCCAGAA GACGGAGCTC 
GGAGCCTTGA TGGGGACCAC GCTCCAGCGT GGGGCTCAGT GGTATCTGAT CGACAGCCGG 
TGGTTCAAGC AGTGGAAGAA GTACGTCGGC TTTGACAGCT GGGACATGTA CAACGTGGGG 
GAGCACAATC TCTTTCCTGG ACCGATTGAC AACTCTGGGC TCTTCTCAGA TCCTGAGAGT 
CAGACCTTGA AGGAACACTT AATCGATGAG CTGGACTATG TGTTGGTTCC AACTGAAGCC 
TGGAATAAAT TGCTAAATTG GTATGGCTGT GTGGAAGGCC AGCAGCCTAT TGTCAGAAAA 
GTTGTGGAGC ATGGCCTGTT TGTCAAGCAC TGCAAAGTGG AAGTGTATTT GCTGGAGTTG 
AAGCTCTGTG AGAACAGTGA CCCCACCAAT GTGCTAAGTT GCCATTTTAG CAAAGCAGAT 
ACCATTGCAA CTATTGAGAA GGAGATGAGG AAGCTCTTCA ACATCCCTGC AGAGCGTGAA 
ACACGACTTT GGAACAAATA CATGAGCAAC ACCTATGAGC AGTTGAGCAA GCTAGACAAC 
ACTATCCAGG ATGCTGGGCT GTACCAGGGT CAGGTGCTAG TAATTGAGCC CCAAAATGAA 
GACGGCACAT GGCCCAGGCA GACCCTGCAA TCAAAATCAA GCACTGCACC TAGCAGAAAT 
TTTACTACCT CTTCAAAACC ATCCGCAAGT CCCTATTCCT CAATGTCTGC CTCTCTCATT 
GCAAATGGTG ATAGCACTAA CAGCTCTGGG ATGCACAACT CCGGTGTCAG CAGGGGTGGA 
GCTGGCTTCT CTGCCTCGTA TAATTGCCAG GAGCCCCCAT CACCTCATAT CCAGCCTGGC 
CTCTGTGGAC TCGGAAACCT GGGAAACACC TGTTTTATGA ATTCTGCTTT GCAGTGCCTG 
AGCAACACTG GTCCACTGAC TGAGTACTTT CTCAAAGATG AGTATGAGGC CGAGATCAAC 
AGAGACAACC CTCTGGGGAT GAAAGGGGAG ATTGCAGAGG CCTATGCAGA GCTCATCAAG 
CAGATGTGGT CTGGAAGGGA CACCCACGTA GCACCACGGA TGTTCAAAAC GCAAGTGGGA 
CGTTTCGCCC CTCAGTTTTC TGGCTACCAA CAACAAGACT CTCAAGAGCT GTTAGCCTTT 
ATTCTAGATG GATTGCATGA AGACCTGAAC CGAGTAAAGA AGAAGCCTTA CCTGGAGCCA 
AAGGATGCCA ATGGGCGACC AGATGCGGTG GTAGCAAAGG AAGCCTGGGA AAATCACAGG 
CTGAGGAATG ACTCTGTGAT TGTGGATACT TTCCATGGCC TTTTCAAATC TACTTTGGTT 
TGCCCAGAAT GTGCTAAAGT TTCTGTGACC TTTGACCCAT TTTGCTATCT AACTCTCCCA 
CTGCCTTTGA AGAAAGATCG AATTATGGAG GTCTTCCTGG TTCCTGCTGA CCCTCATTGC 
AGACCTATCC AGTACCGTGT GACTGTGCCA TTGATGGGGG CCATTTCTGA CCTGTGTGAA 
GCACTCTCCA AGCTGTCTGG CATTGCTGCA GAAAATATGG TGGTCACTGA TGTGTATAAT 
CACCGCTTCC ACAAAATTTT CCAAATGGAT GAAGGTTTAA GCCACATCAC GCCTCGAGAT 
GACATTTTTG TGTATGAGAT CTGCACCACT CCCATGGATG GCTCAGAATA TATCACTCTC 
CCAGTCTACT TCAGAGAAAA GAAGTCCAGG CCATCGAGTA CTTCCTCTGG GGCCGTGCTC 
TATGGACAGC CACTTCTTGT TTCTGTCCCT AAGCACAGAC TAACCCTCGA GTCCTTATAC 
CAGGCTGTTT GTGAACGTAT CAGCCGCTAT ATAAAGCAGC CTTTGCCTGA GGAGTTTCTC 
AGCTCTCCCT TAGAGCCTGG GGCCTGCAAT GGCTCTAGGG GTAGCTATGA AGGAGATGAA 
GAAGAAATGG ATCATCAAGA GGAAGGGAAA GAACAGCTTT CTGAAGTGGA AGAAAGTGGT 
GAGGACAGTC AGGGAGGGGA CCCTACTGAG ACCACCCAAA AGGCGAAAGG CCCGCCCCGC 
CACAAGAGGC TTTTCACCTT CAGCCTCGTG AACTCCTGTG GAACTGCTGA TATCAACTCG 
CTGGCCACTG ACGGAAAACT CCTCAAGCTC AACTCTCGAT CCACACTGGC CATTGACTGG 
GACAGTGAGA CCCGAAGCCT TTACTTTGAT GAGCAAGAAT CTGAGGCCTG TGAGAAGCAC 
ACGAGCATGT CCCAGCCTCA GAAGAAGAAG AAGGCTGCAA TAGCCCTGCG GGAGTGCATC 
GAGCTCTTTA CTACCATGGA GACCCTTGGG GAGCATGACC CCTGGTACTG TCCCACCTGT 
AAGAAGCACC AACAGGCAAC AAAAAAGTTT GATTTGTGGT CTTTGCCCAA GATCCTGGTG 
GTTCATCTCA AACGTTTCTC CTATAACAGA TACTGGCGGG ATAAACTGGA CACAGTTGTG 
GAATTCCCAG TAAGAGCTCT GAACATGTCC GAGTTTGTGT GTGACCGGGC AGCAAGGCCT 
TATGTCTATG ACCTGATTGC TGTGTCCAAT CACTATGGCG CTATGGGGGT CGGTCACTAT 
ACTGCATATG CGAAGAACAG ACTGAATGGG AAATGGTATT ACTTTGATGA TAGCAGCGTG 
TCCCTGGCCT CTGAGGATCA GATAGTGACG AAGGCCGCCT ACGTGTTGTT TTACCAGCGT 
CGGGATGATG AGTGTCCCAG CACCTCCTCA CCTGTCAGCT TCCCGGGTTC TGATGGAGGG 
GCGAAGCTCA GCAGCTCACA GCAGGACTTG GGGGAAGAGG AGGCTTACAC CATGGACACC 
AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001128484.1
CDS	25..2910
Translation	MAEGRGTHERPDVETQKTELGALMGTTLQRGAQWYLIDSRWFKQWKKYVGFDSWDMYNVGEHNLFPGPIDNSGLFSDPESQTLKEHLIDELDYVLVPTEAWNKLLNWYGCVEGQQPIVRKVVEHGLFVKHCKVEVYLLELKLCENSDPTNVLSCHFSKADTIATIEKEMRKLFNIPAERETRLWNKYMSNTYEQLSKLDNTIQDAGLYQGQVLVIEPQNEDGTWPRQTLQSKSSTAPSRNFTTSSKPSASPYSSMSASLIANGDSTNSSGMHNSGVSRGGAGFSASYNCQEPPSPHIQPGLCGLGNLGNTCFMNSALQCLSNTGPLTEYFLKDEYEAEINRDNPLGMKGEIAEAYAELIKQMWSGRDTHVAPRMFKTQVGRFAPQFSGYQQQDSQELLAFILDGLHEDLNRVKKKPYLEPKDANGRPDAVVAKEAWENHRLRNDSVIVDTFHGLFKSTLVCPECAKVSVTFDPFCYLTLPLPLKKDRIMEVFLVPADPHCRPIQYRVTVPLMGAISDLCEALSKLSGIAAENMVVTDVYNHRFHKIFQMDEGLSHITPRDDIFVYEICTTPMDGSEYITLPVYFREKKSRPSSTSSGAVLYGQPLLVSVPKHRLTLESLYQAVCERISRYIKQPLPEEFLSSPLEPGACNGSRGSYEGDEEEMDHQEEGKEQLSEVEESGEDSQGGDPTETTQKAKGPPRHKRLFTFSLVNSCGTADINSLATDGKLLKLNSRSTLAIDWDSETRSLYFDEQESEACEKHTSMSQPQKKKKAAIALRECIELFTTMETLGEHDPWYCPTCKKHQQATKKFDLWSLPKILVVHLKRFSYNRYWRDKLDTVVEFPVRALNMSEFVCDRAARPYVYDLIAVSNHYGAMGVGHYTAYAKNRLNGKWYYFDDSSVSLASEDQIVTKAAYVLFYQRRDDECPSTSSPVSFPGSDGGAKLSSSQQDLGEEEAYTMDTN

Target ORF information:

RefSeq Version	NM_001135012.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ubiquitin specific peptidase 4 (Usp4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001135012.1

ORF Insert Sequence:

ATGGCGGAAG GCCGGGGCAC CCATGAGCGA CCGGATGTGG AGACCCAGAA GACGGAGCTC 
GGAGCCTTGA TGGGGACCAC GCTCCAGCGT GGGGCTCAGT GGTATCTGAT CGACAGCCGG 
TGGTTCAAGC AGTGGAAGAA GTACGTCGGC TTTGACAGCT GGGACATGTA CAACGTGGGG 
GAGCACAATC TCTTTCCTGG ACCGATTGAC AACTCTGGGC TCTTCTCAGA TCCTGAGAGT 
CAGACCTTGA AGGAACACTT AATCGATGAG CTGGACTATG TGTTGGTTCC AACTGAAGCC 
TGGAATAAAT TGCTAAATTG GTATGGCTGT GTGGAAGGCC AGCAGCCTAT TGTCAGAAAA 
GTTGTGGAGC ATGGCCTGTT TGTCAAGCAC TGCAAAGTGG AAGTGTATTT GCTGGAGTTG 
AAGCTCTGTG AGAACAGTGA CCCCACCAAT GTGCTAAGTT GCCATTTTAG CAAAGCAGAT 
ACCATTGCAA CTATTGAGAA GGAGATGAGG AAGCTCTTCA ACATCCCTGC AGAGCGTGAA 
ACACGACTTT GGAACAAATA CATGAGCAAC ACCTATGAGC AGTTGAGCAA GCTAGACAAC 
ACTATCCAGG ATGCTGGGCT GTACCAGGGT CAGGTGCTAG TAATTGAGCC CCAAAATGAA 
GACGGCACAT GGCCCAGGCA GACCCTGCAA TCAAAATCAA GCACTGCACC TAGCAGAAAT 
TTTACTACCT CTTCAAAACC ATCCGCAAGT CCCTATTCCT CAATGTCTGC CTCTCTCATT 
GCAAATGGTG ATAGCACTAA CAGCTCTGGG ATGCACAACT CCGGTGTCAG CAGGGGTGGA 
GCTGGCTTCT CTGCCTCGTA TAATTGCCAG GAGCCCCCAT CACCTCATAT CCAGCCTGGC 
CTCTGTGGAC TCGGAAACCT GGGAAACACC TGTTTTATGA ATTCTGCTTT GCAGTGCCTG 
AGCAACACTG GTCCACTGAC TGAGTACTTT CTCAAAGATG AGTATGAGGC CGAGATCAAC 
AGAGACAACC CTCTGGGGAT GAAAGGGGAG ATTGCAGAGG CCTATGCAGA GCTCATCAAG 
CAGATGTGGT CTGGAAGGGA CACCCACGTA GCACCACGGA TGTTCAAAAC GCAAGTGGGA 
CGTTTCGCCC CTCAGTTTTC TGGCTACCAA CAACAAGACT CTCAAGAGCT GTTAGCCTTT 
ATTCTAGATG GATTGCATGA AGACCTGAAC CGAGTAAAGA AGAAGCCTTA CCTGGAGCCA 
AAGGATGCCA ATGGGCGACC AGATGCGGTG GTAGCAAAGG AAGCCTGGGA AAATCACAGG 
CTGAGGAATG ACTCTGTGAT TGTGGATACT TTCCATGGCC TTTTCAAATC TACTTTGGTT 
TGCCCAGAAT GTGCTAAAGT TTCTGTGACC TTTGACCCAT TTTGCTATCT AACTCTCCCA 
CTGCCTTTGA AGAAAGATCG AATTATGGAG GTCTTCCTGG TTCCTGCTGA CCCTCATTGC 
AGACCTATCC AGTACCGTGT GACTGTGCCA TTGATGGGGG CCATTTCTGA CCTGTGTGAA 
GCACTCTCCA AGCTGTCTGG CATTGCTGCA GAAAATATGG TGGTCACTGA TGTGTATAAT 
CACCGCTTCC ACAAAATTTT CCAAATGGAT GAAGGTTTAA GCCACATCAC GCCTCGAGAT 
GACATTTTTG TGTATGAGAT CTGCACCACT CCCATGGATG GCTCAGAATA TATCACTCTC 
CCAGTCTACT TCAGAGAAAA GAAGTCCAGG CCATCGAGTA CTTCCTCTGG GGCCGTGCTC 
TATGGACAGC CACTTCTTGT TTCTGTCCCT AAGCACAGAC TAACCCTCGA GTCCTTATAC 
CAGGCTGTTT GTGAACGTAT CAGCCGCTAT ATAAAGCAGC CTTTGCCTGA GGAGTTTCTC 
AGCTCTCCCT TAGAGCCTGG GGCCTGCAAT GGCTCTAGGG GTAGCTATGA AGGAGATGAA 
GAAGAAATGG ATCATCAAGA GGAAGGGAAA GAACAGCTTT CTGAAGTGGA AGAAAGTGGT 
GAGGACAGTC AGGGAGGGGA CCCTACTGAG ACCACCCAAA AGGCGAAAGG CCCGCCCCGC 
CACAAGAGGC TTTTCACCTT CAGCCTCGTG AACTCCTGTG GAACTGCTGA TATCAACTCG 
CTGGCCACTG ACGGAAAACT CCTCAAGCTC AACTCTCGAT CCACACTGGC CATTGACTGG 
GACAGTGAGA CCCGAAGCCT TTACTTTGAT GAGCAAGAAT CTGAGGCCTG TGAGAAGCAC 
ACGAGCATGT CCCAGCCTCA GAAGAAGAAG AAGGCTGCAA TAGCCCTGCG GGAGTGCATC 
GAGCTCTTTA CTACCATGGA GACCCTTGGG GAGCATGACC CCTGGTACTG TCCCACCTGT 
AAGAAGCACC AACAGGCAAC AAAAAAGTTT GATTTGTGGT CTTTGCCCAA GATCCTGGTG 
GTTCATCTCA AACGTTTCTC CTATAACAGA TACTGGCGGG ATAAACTGGA CACAGTTGTG 
GAATTCCCAG TAAGAGCTCT GAACATGTCC GAGTTTGTGT GTGACCGGGC AGCAAGGCCT 
TATGTCTATG ACCTGATTGC TGTGTCCAAT CACTATGGCG CTATGGGGGT CGGTCACTAT 
ACTGCATATG CGAAGAACAG ACTGAATGGG AAATGGTATT ACTTTGATGA TAGCAGCGTG 
TCCCTGGCCT CTGAGGATCA GATAGTGACG AAGGCCGCCT ACGTGTTGTT TTACCAGCGT 
CGGGATGATG AGTGTCCCAG CACCTCCTCA CCTGTCAGCT TCCCGGGTTC TGATGGAGGG 
GCGAAGCTCA GCAGCTCACA GCAGGACTTG GGGGAAGAGG AGGCTTACAC CATGGACACC 
AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Downregulation of USP4 Promotes Activation of Microglia and Subsequent Neuronal Inflammation in Rat Spinal Cord After Injury.
Neurochemical research42(11)3245-3253(2017 Nov)
Jiang X,Yu M,Ou Y,Cao Y,Yao Y,Cai P,Zhang F

Increased Expression of Ubiquitin-Specific Protease 4 Participates in Neuronal Apoptosis After Intracerebral Hemorrhage in Adult Rats.
Cellular and molecular neurobiology37(3)427-435(2017 Apr)
Liu C,Liu C,Liu H,Gong L,Tao T,Shen Y,Zhu S,Shen A

Structural variability of the ubiquitin specific protease DUSP-UBL double domains.
FEBS letters585(21)3385-90(2011 Nov)
Elliott PR,Liu H,Pastok MW,Grossmann GJ,Rigden DJ,Clague MJ,Urb? S,Barsukov IL

The Prp19 complex and the Usp4Sart3 deubiquitinating enzyme control reversible ubiquitination at the spliceosome.
Genes & development24(13)1434-47(2010 Jul)
Song EJ,Werner SL,Neubauer J,Stegmeier F,Aspden J,Rio D,Harper JW,Elledge SJ,Kirschner MW,Rape M

UnpEL/Usp4 is ubiquitinated by Ro52 and deubiquitinated by itself.
Biochemical and biophysical research communications342(1)253-8(2006 Mar)
Wada K,Kamitani T

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Usp4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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