Pgpep1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pgpep1
Entrez Gene ID	290648
Full Name	pyroglutamyl-peptidase I
Synonyms	PAPI
General protein information	Preferred Names pyroglutamyl-peptidase I Names pyroglutamyl-peptidase 1 5-oxoprolyl-peptidase PAP-I PGP-I pyroglutamyl aminopeptidase I pyrrolidone-carboxylate peptidase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 16 Map Location: 16p14
Summary	catalyzes the hydrolysis of the L-pyroglutamate residue from the amino terminus of proteins and peptides [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_201988.2	NP_973717.1	pyroglutamyl-peptidase 1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Pgpep1	NP_075706.1
Rattus norvegicus (Norway rat)	Pgpep1	NP_973717.1
Gallus gallus (chicken)	PGPEP1	XP_418244.4
Danio rerio (zebrafish)	pgpep1	NP_001018362.1
Caenorhabditis elegans (roundworm)	M04C9.1	NP_001122498.1
Homo sapiens (human)	PGPEP1	NP_060182.1
Pan troglodytes (chimpanzee)	PGPEP1	XP_001162684.1
Drosophila melanogaster (fruit fly)	CG32147	NP_730035.1
Caenorhabditis elegans (roundworm)	F35F10.6	NP_503910.2
	PGPEP1	XP_001115337.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006964	XP_308793.4
Caenorhabditis elegans (roundworm)	M04C9.3	NP_492491.2
Caenorhabditis elegans (roundworm)	M04C9.2	NP_492489.2
Caenorhabditis elegans (roundworm)	CELE_F16H6.7	NP_507562.2
Xenopus tropicalis (tropical clawed frog)	pgpep1	NP_001006752.1
Bos taurus (cattle)	PGPEP1	NP_001103453.1

Gene Ontology
Bibliography

Related articles in PubMed

Continuous stress-induced dopamine dysregulation augments PAP-I and PAP-II expression in melanotrophs of the pituitary gland.
Konishi H, Ogawa T, Kawahara S, Matsumoto S, Kiyama H
Biochemical and biophysical research communications407(1)7-12(2011 Apr)

Hydrolysis of synthetic substrate, L-pyroglutamyl p-nitroanilide is catalyzed solely by pyroglutamyl aminopeptidase I in rat liver cytosol.
Abe K, Watanabe N, Kosaka T, Yamada M, Tokui T, Ikeda T
Biological & pharmaceutical bulletin26(11)1528-33(2003 Nov)

Serum pyrrolidone carboxypeptidase activity in N-methyl-nitrosourea induced rat breast cancer.
Carrera MP, Ram?rez-Exp?sito MJ, Valenzuela MT, Garc?a MJ, Mayas MD, Mart?nez-Martos JM
Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme35(8)502-5(2003 Aug)

Effect of the disruption of body fluid balance on pyroglutamyl aminopeptidase (Type-1) in rat brain structures.
Irazusta J, Gil J, Ruiz F, Agirregoitia N, Casis L, Silveira PF
Neuropeptides36(5)333-40(2002 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

PAP-I and PAP-II expression was induced by the decreased dopamine levels in the intermediate lobe of the pituitary under continuous stress.
Title: Continuous stress-induced dopamine dysregulation augments PAP-I and PAP-II expression in melanotrophs of the pituitary gland.

Significant correlation between Pcp activity and the number and size of tumours and the body weight of the animals. Decreased Pcp may reflect increase in circulating GnRH leading to increase in gonadal steroid hormones.
Title: Serum pyrrolidone carboxypeptidase activity in N-methyl-nitrosourea induced rat breast cancer.

Pyroglutamyl aminopeptidase Type-1 shows differentiated activity in various parts of the brain under varied conditions of hydration in rats.
Title: Effect of the disruption of body fluid balance on pyroglutamyl aminopeptidase (Type-1) in rat brain structures.

The following Pgpep1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pgpep1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa05481	NM_201988.2 Latest version!	Rattus norvegicus pyroglutamyl-peptidase I (Pgpep1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pgpep1 ( NM_201988.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_973717.1 Rattus norvegicus pyroglutamyl-peptidase I (Pgpep1), mRNA.

Price & Availability↑

CloneID	ORa05481
Clone ID Related Accession (Same CDS sequence)	NM_201988.2
Accession Version	NM_201988.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 630bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Rattus norvegicus(Norway rat)
Product	pyroglutamyl-peptidase 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC098645.1. On Jul 31, 2005 this sequence version replaced NM_201988.1. ##Evidence-Data-START## Transcript exon combination :: BC098645.1, AB098134.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGAGCAGC CGCGGAAAGC GGTAGTGGTG ACCGGATTCG GCCCTTTTGG GGAGCATGCT 
GTGAATGCCA GCTGGATCGC TGTCCAGGAG CTAGAGAAAC TGGGCCTTGG GGACAGCGTG 
GACCTGCACG TGTATGAGAT CCCTGTGGAG TACCAAACTG TGCAGAGGCT CATCCCAGCG 
CTGTGGGAGA AACACAGCCC CCAGCTCGTG GTGCATGTCG GGGTGTCAGG CATGGCCACC 
ACAGTGACGC TGGAAAAATG TGGCCACAAC AAGGGTTACA AAGGACTGGA TAATTGCCGG 
TTCTGCCCCG GCTCGCAGTG CTGCGTGGAG GATGGTCCAG AGAGCATTGA CTCCATCATC 
GACATGGACG CTGTGTGCAA AAGGGTGACC ACACTGGGGC TGGACGTGTC TGTTACCATC 
TCCCAGGATG CTGGCAGATA CCTGTGTGAC TTCACATATT ACACGTCGCT CTACCGGGGC 
CGAGGCCGCT CAGCTTTTGT CCATGTGCCC CCCCTGGGCA AGCCTTACAA TGCTGACCAG 
CTAGGCCGGG CACTACGGGC CATCATTGAA GAGATGCTGG GTGTTCTAGA GCAGGCAGAG 
GGAGACATCA GCTGCTGCCA CCAGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_973717.1
CDS	19..648
Translation	MEQPRKAVVVTGFGPFGEHAVNASWIAVQELEKLGLGDSVDLHVYEIPVEYQTVQRLIPALWEKHSPQLVVHVGVSGMATTVTLEKCGHNKGYKGLDNCRFCPGSQCCVEDGPESIDSIIDMDAVCKRVTTLGLDVSVTISQDAGRYLCDFTYYTSLYRGRGRSAFVHVPPLGKPYNADQLGRALRAIIEEMLGVLEQAEGDISCCHQL

Target ORF information:

RefSeq Version	NM_201988.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus pyroglutamyl-peptidase I (Pgpep1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_201988.2

ORF Insert Sequence:

ATGGAGCAGC CGCGGAAAGC GGTAGTGGTG ACCGGATTCG GCCCTTTTGG GGAGCATGCT 
GTGAATGCCA GCTGGATCGC TGTCCAGGAG CTAGAGAAAC TGGGCCTTGG GGACAGCGTG 
GACCTGCACG TGTATGAGAT CCCTGTGGAG TACCAAACTG TGCAGAGGCT CATCCCAGCG 
CTGTGGGAGA AACACAGCCC CCAGCTCGTG GTGCATGTCG GGGTGTCAGG CATGGCCACC 
ACAGTGACGC TGGAAAAATG TGGCCACAAC AAGGGTTACA AAGGACTGGA TAATTGCCGG 
TTCTGCCCCG GCTCGCAGTG CTGCGTGGAG GATGGTCCAG AGAGCATTGA CTCCATCATC 
GACATGGACG CTGTGTGCAA AAGGGTGACC ACACTGGGGC TGGACGTGTC TGTTACCATC 
TCCCAGGATG CTGGCAGATA CCTGTGTGAC TTCACATATT ACACGTCGCT CTACCGGGGC 
CGAGGCCGCT CAGCTTTTGT CCATGTGCCC CCCCTGGGCA AGCCTTACAA TGCTGACCAG 
CTAGGCCGGG CACTACGGGC CATCATTGAA GAGATGCTGG GTGTTCTAGA GCAGGCAGAG 
GGAGACATCA GCTGCTGCCA CCAGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Continuous stress-induced dopamine dysregulation augments PAP-I and PAP-II expression in melanotrophs of the pituitary gland.
Biochemical and biophysical research communications407(1)7-12(2011 Apr)
Konishi H,Ogawa T,Kawahara S,Matsumoto S,Kiyama H

Hydrolysis of synthetic substrate, L-pyroglutamyl p-nitroanilide is catalyzed solely by pyroglutamyl aminopeptidase I in rat liver cytosol.
Biological & pharmaceutical bulletin26(11)1528-33(2003 Nov)
Abe K,Watanabe N,Kosaka T,Yamada M,Tokui T,Ikeda T

Serum pyrrolidone carboxypeptidase activity in N-methyl-nitrosourea induced rat breast cancer.
Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme35(8)502-5(2003 Aug)
Carrera MP,Ram?rez-Exp?sito MJ,Valenzuela MT,Garc?a MJ,Mayas MD,Mart?nez-Martos JM

Effect of the disruption of body fluid balance on pyroglutamyl aminopeptidase (Type-1) in rat brain structures.
Neuropeptides36(5)333-40(2002 Oct)
Irazusta J,Gil J,Ruiz F,Agirregoitia N,Casis L,Silveira PF