Tnnc1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tnnc1
Entrez Gene ID	290561
Full Name	troponin C1, slow skeletal and cardiac type
Synonyms	Tncc
General protein information	Preferred Names troponin C1, slow skeletal and cardiac type Names troponin C, slow skeletal and cardiac muscles troponin C type 1 (slow) troponin C, cardiac/slow skeletal
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 16 Map Location: 16p16

mRNA and Protein(s)

mRNA	Protein	Name
NM_001034105.1	NP_001029277.1	troponin C, slow skeletal and cardiac muscles

Pathways from BioSystems

Homologies

	LOC697047	XP_001085656.2
Canis lupus familiaris (dog)	TNNC1	XP_005632514.1
Homo sapiens (human)	TNNC1	NP_003271.1
Xenopus tropicalis (tropical clawed frog)	tnnc1	NP_001004776.1
Bos taurus (cattle)	TNNC1	NP_001029523.1
Rattus norvegicus (Norway rat)	Tnnc1	NP_001029277.1
Gallus gallus (chicken)	TNNC1	NP_990464.1
Pan troglodytes (chimpanzee)	TNNC1	XP_003950175.1
Mus musculus (house mouse)	Tnnc1	NP_033419.1
Danio rerio (zebrafish)	tnnc1a	NP_852475.2

Gene Ontology
Bibliography

Related articles in PubMed

The concerted movement of the switch region of Troponin I in cardiac muscle thin filaments as tracked by conventional and pulsed (DEER) EPR.
Potluri PR, Chamoun J, Cooke JA, Badr M, Guse JA, Rayes R, Cordina NM, McCamey D, Fajer PG, Brown LJ
Journal of structural biology200(3)376-387(2017 Dec)

Sarcomere length dependent effects on the interaction between cTnC and cTnI in skinned papillary muscle strips.
Li KL, Ghashghaee NB, Solaro RJ, Dong W
Archives of biochemistry and biophysics60169-79(2016 Jul)

Probing the mechanism of cardiovascular drugs using a covalent levosimendan analog.
Pineda-Sanabria SE, Robertson IM, Sun YB, Irving M, Sykes BD
Journal of molecular and cellular cardiology92174-84(2016 Mar)

A novel phosphorylation site, Serine 199, in the C-terminus of cardiac troponin I regulates calcium sensitivity and susceptibility to calpain-induced proteolysis.
Wijnker PJ, Li Y, Zhang P, Foster DB, dos Remedios C, Van Eyk JE, Stienen GJ, Murphy AM, van der Velden J
Journal of molecular and cellular cardiology8293-103(2015 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the interaction between cTnC and cTnI in skinned papillary muscle strips is dependent on sarcomere length
Title: Sarcomere length dependent effects on the interaction between cTnC and cTnI in skinned papillary muscle strips.

These findings provide new insights into the in vivo mechanism of Ca(2+) sensitization and demonstrate that directly targeting cTnC has significant potential in cardiovascular therapy.
Title: Probing the mechanism of cardiovascular drugs using a covalent levosimendan analog.

These findings supported our hypothesis here that strong cross-bridge interactions with the cardiac thin filament exert a Ca(2+)-sensitizing effect on the CRS.
Title: Structural basis for the in situ Ca(2+) sensitization of cardiac troponin C by positive feedback from force-generating myosin cross-bridges.

the inhibitory effect of EGCg on myofilament Ca(2+) activation may be related to an enhanced Ca(2+)-Mg(2+)exchange at sites III and IV of cTnC, which might reduce the myosin crossbridge dependent component of thin filament activation.
Title: The green tea polyphenol (-)-epigallocatechin-3-gallate inhibits magnesium binding to the C-domain of cardiac troponin C.

Calcium Binding Changes the Open-Closed Equilibrium of the regulatory N-domain of isolated cardiac troponin C.
Title: Effects of calcium binding and the hypertrophic cardiomyopathy A8V mutation on the dynamic equilibrium between closed and open conformations of the regulatory N-domain of isolated cardiac troponin C.

The following Tnnc1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tnnc1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa07426	NM_001034105.1 Latest version!	Rattus norvegicus troponin C1, slow skeletal and cardiac type (Tnnc1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
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5 or more Clone	50% OFF
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30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tnnc1 ( NM_001034105.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001029277.1 Rattus norvegicus troponin C1, slow skeletal and cardiac type (Tnnc1), mRNA.

Price & Availability↑

CloneID	ORa07426
Clone ID Related Accession (Same CDS sequence)	NM_001034105.1
Accession Version	NM_001034105.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 486bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Rattus norvegicus(Norway rat)
Product	troponin C, slow skeletal and cardiac muscles
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DQ062205.1. On Oct 14, 2005 this sequence version replaced XM_214266.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ062205.1, CR753959.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689596, SAMEA2689597 [ECO:0000348] ##Evidence-Data-END##

ATGGATGACA TCTACAAAGC TGCGGTAGAG CAGTTGACAG AAGAACAGAA GAATGAGTTC 
AAGGCTGCCT TCGATATCTT TGTCCTGGGC GCGGAGGATG GCTGCATCAG CACCAAGGAG 
CTGGGCAAGG TGATGAGGAT GCTGGGACAG AACCCCACAC CTGAGGAGCT GCAGGAGATG 
ATCGACGAGG TAGACGAGGA TGGCAGTGGC ACAGTGGACT TCGACGAGTT CCTTGTCATG 
ATGGTTCGGT GCATGAAGGA TGACAGCAAA GGGAAGTCTG AGGAGGAGCT GTCGGATCTC 
TTCCGCATGT TTGACAAAAA CGCTGATGGC TACATTGACT TGGATGAACT GAAGATGATG 
CTGCAGGCCA CAGGTGAGAC CATCACGGAA GATGACATCG AAGAGCTCAT GAAGGACGGT 
GACAAGAACA ACGATGGCCG AATCGACTAT GACGAGTTCC TGGAATTCAT GAAGGGTGTG 
GAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001029277.1
CDS	1..486
Translation	MDDIYKAAVEQLTEEQKNEFKAAFDIFVLGAEDGCISTKELGKVMRMLGQNPTPEELQEMIDEVDEDGSGTVDFDEFLVMMVRCMKDDSKGKSEEELSDLFRMFDKNADGYIDLDELKMMLQATGETITEDDIEELMKDGDKNNDGRIDYDEFLEFMKGVE

Target ORF information:

RefSeq Version	NM_001034105.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus troponin C1, slow skeletal and cardiac type (Tnnc1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001034105.1

ORF Insert Sequence:

ATGGATGACA TCTACAAAGC TGCGGTAGAG CAGTTGACAG AAGAACAGAA GAATGAGTTC 
AAGGCTGCCT TCGATATCTT TGTCCTGGGC GCGGAGGATG GCTGCATCAG CACCAAGGAG 
CTGGGCAAGG TGATGAGGAT GCTGGGACAG AACCCCACAC CTGAGGAGCT GCAGGAGATG 
ATCGACGAGG TAGACGAGGA TGGCAGTGGC ACAGTGGACT TCGACGAGTT CCTTGTCATG 
ATGGTTCGGT GCATGAAGGA TGACAGCAAA GGGAAGTCTG AGGAGGAGCT GTCGGATCTC 
TTCCGCATGT TTGACAAAAA CGCTGATGGC TACATTGACT TGGATGAACT GAAGATGATG 
CTGCAGGCCA CAGGTGAGAC CATCACGGAA GATGACATCG AAGAGCTCAT GAAGGACGGT 
GACAAGAACA ACGATGGCCG AATCGACTAT GACGAGTTCC TGGAATTCAT GAAGGGTGTG 
GAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The concerted movement of the switch region of Troponin I in cardiac muscle thin filaments as tracked by conventional and pulsed (DEER) EPR.
Journal of structural biology200(3)376-387(2017 Dec)
Potluri PR,Chamoun J,Cooke JA,Badr M,Guse JA,Rayes R,Cordina NM,McCamey D,Fajer PG,Brown LJ

Sarcomere length dependent effects on the interaction between cTnC and cTnI in skinned papillary muscle strips.
Archives of biochemistry and biophysics60169-79(2016 Jul)
Li KL,Ghashghaee NB,Solaro RJ,Dong W

Probing the mechanism of cardiovascular drugs using a covalent levosimendan analog.
Journal of molecular and cellular cardiology92174-84(2016 Mar)
Pineda-Sanabria SE,Robertson IM,Sun YB,Irving M,Sykes BD

A novel phosphorylation site, Serine 199, in the C-terminus of cardiac troponin I regulates calcium sensitivity and susceptibility to calpain-induced proteolysis.
Journal of molecular and cellular cardiology8293-103(2015 May)
Wijnker PJ,Li Y,Zhang P,Foster DB,dos Remedios C,Van Eyk JE,Stienen GJ,Murphy AM,van der Velden J