Lefty2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Lefty2
Entrez Gene ID	289316
Full Name	Left-right determination factor 2
Synonyms	Ebaf2
General protein information	Preferred Names Left-right determination factor 2 Names left-right determination factor 2 endometrial bleeding associated factor
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 13 Map Location: 13q26

mRNA and Protein(s)

mRNA	Protein	Name
NM_001007556.1	NP_001007557.1	left-right determination factor 2 precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	LEFTY2	NP_003231.2
Pan troglodytes (chimpanzee)	LEFTY2	XP_514238.2
Rattus norvegicus (Norway rat)	Lefty2	NP_001007557.1
Bos taurus (cattle)	LEFTY2	NP_001192972.1
Mus musculus (house mouse)	Lefty2	NP_796073.1
	LEFTY2	XP_001092296.1
Canis lupus familiaris (dog)	LEFTY2	XP_547508.3
Bos taurus (cattle)	LOC100336971	XP_005216922.1

Gene Ontology
Bibliography

Related articles in PubMed

LEFTY2 expression and localization in rat oviduct during early pregnancy.
Arga?araz ME, Apichela SA, Miceli DC
Zygote (Cambridge, England)20(1)53-60(2012 Feb)

Rat Lefty2/EBAF gene: isolation and expression analysis in the oviduct during the early pregnancy stage.
Arga?araz ME, Valdecantos PA, Abate CM, Miceli DC
Genes & genetic systems82(2)171-5(2007 Apr)

Regulation and function of LEFTY-A/EBAF in the human endometrium. mRNA expression during the menstrual cycle, control by progesterone, and effect on matrix metalloprotineases.
Cornet PB, Picquet C, Lemoine P, Osteen KG, Bruner-Tran KL, Tabibzadeh S, Courtoy PJ, Eeckhout Y, Marbaix E, Henriet P
The Journal of biological chemistry277(45)42496-504(2002 Nov)

Dysregulated expression of ebaf, a novel molecular defect in the endometria of patients with infertility.
Tabibzadeh S, Mason JM, Shea W, Cai Y, Murray MJ, Lessey B
The Journal of clinical endocrinology and metabolism85(7)2526-36(2000 Jul)

Two closely-related left-right asymmetrically expressed genes, lefty-1 and lefty-2: their distinct expression domains, chromosomal linkage and direct neuralizing activity in Xenopus embryos.
Meno C, Ito Y, Saijoh Y, Matsuda Y, Tashiro K, Kuhara S, Hamada H
Genes to cells : devoted to molecular & cellular mechanisms2(8)513-24(1997 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

LEFTY2 is expressed at a high level just before the embryos pass to the uterus; its biological effect might be relevant and significant for the preimplantation stage of embryo development in the oviduct.
Title: LEFTY2 expression and localization in rat oviduct during early pregnancy.

The highest expression of lefty2 was at the 4(th) day after mating (five folds respect to day one), just when the embryos have completed their transit through the oviduct.
Title: Rat Lefty2/EBAF gene: isolation and expression analysis in the oviduct during the early pregnancy stage.

The following Lefty2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Lefty2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa41887	NM_001007556.1 Latest version!	Rattus norvegicus Left-right determination factor 2 (Lefty2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Lefty2 ( NM_001007556.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001007557.1 Rattus norvegicus Left-right determination factor 2 (Lefty2), mRNA.

Price & Availability↑

CloneID	ORa41887
Clone ID Related Accession (Same CDS sequence)	NM_001007556.1
Accession Version	NM_001007556.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1101bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-26
Organism	Rattus norvegicus(Norway rat)
Product	left-right determination factor 2 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY758558.1. On Nov 29, 2004 this sequence version replaced XM_222999.1. ##Evidence-Data-START## Transcript exon combination :: AY758558.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689601, SAMN02642721 [ECO:0000348] ##Evidence-Data-END##

ATGCAGTCCC TGTGGCTTTG CTGGACACTC TGCGTACTGC CCCTGGCTGG CCCTGGGGCA 
GCGGTGACTG AGGAACAGGT TCTGAGCAGT CTCCTGAAGC AGCTGCAGCT CAGCCAGGCC 
CCGGTCCTGG ACAGAGTGGA TGTGGAGGGG ATGGCCATCC CTACCCATGT GAGCTCCCAG 
TATGTGGCCC TGCTGCAGGG AAGTCATGCT GACCGCTCCC GAGGCAAGAG GTTCAGCCAG 
AACTTTCGAG AGGTGGCAGG CAGGTTCCTG GTGTCAGAGA CCTCCAGTCA CCTGCTGGTG 
TTCGGCATGG AGCAGCGGCT GCCGCCTAAC AGTGAGCTGG TGCAGGCCAT GCTGCGGCTG 
TTCCAGGAGC CCGTGCCCAG AACAGCTCTC CGGAGGCTTG AGAGGCTGCC CCCTCATAGT 
GCCCAAGCTC GGGTCACCAT CGAGTGGCTG AGAGTCCGTG AGGATGGCTC CAACCGCACC 
GCCCTCATCG ATTCCAGGCT CGTGTCCATC TACGAGAGCG GCTGGAAGGT CTTCGATGTG 
ACCGAGGCCG TGAACTTCTG GCAGCAGCTG AGCCGGCCGA GGCAGCCGCT GCTGCTCCAG 
GTGTCGGTGC AGAGGGAGCA TCTGGGACCG GGGACCTGGA GCGCACACAA GTTGGTCCGG 
TTCGCGGCGC AGGGGACGCC GGACGGCAAG GGCGAGCCAC AGCTGGAACT GCACACGCTG 
GACCTCAAGG ACTACGGAGC TCAAGGCAAT TGTGACCCCG AGGCACCAGT GACTGAAGGC 
ACCCGATGCT GTCGCAAGGA GATGTACCTG GACCTGCAGG GGATGAAGTG GGCTGAGAAC 
TGGATCCTAG AACCCCCAGG GTTCCTCATT TATGAATGTG TGGGCAGCTG CCGGCAGCTG 
CCAGAGTCTC TGACCATCGG GTGGCCATTT CTGGGGCCTC GGCAGTGTGT GGCCTCAGAG 
ATGACTTCCC TGCCCATGAT TGTCAGCATA AAGGAGGACG GCAAGACCAG GCCTCAAGTG 
GTCAGCCTGC CCAACATGAG GGTGCAGACG TGTAGTTGTG CCTCGGATGG GGCACTCATA 
CCTAGGGGGA TAGATCTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001007557.1
CDS	65..1165
Translation	MQSLWLCWTLCVLPLAGPGAAVTEEQVLSSLLKQLQLSQAPVLDRVDVEGMAIPTHVSSQYVALLQGSHADRSRGKRFSQNFREVAGRFLVSETSSHLLVFGMEQRLPPNSELVQAMLRLFQEPVPRTALRRLERLPPHSAQARVTIEWLRVREDGSNRTALIDSRLVSIYESGWKVFDVTEAVNFWQQLSRPRQPLLLQVSVQREHLGPGTWSAHKLVRFAAQGTPDGKGEPQLELHTLDLKDYGAQGNCDPEAPVTEGTRCCRKEMYLDLQGMKWAENWILEPPGFLIYECVGSCRQLPESLTIGWPFLGPRQCVASEMTSLPMIVSIKEDGKTRPQVVSLPNMRVQTCSCASDGALIPRGIDL

Target ORF information:

RefSeq Version	NM_001007556.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus Left-right determination factor 2 (Lefty2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001007556.1

ORF Insert Sequence:

ATGCAGTCCC TGTGGCTTTG CTGGACACTC TGCGTACTGC CCCTGGCTGG CCCTGGGGCA 
GCGGTGACTG AGGAACAGGT TCTGAGCAGT CTCCTGAAGC AGCTGCAGCT CAGCCAGGCC 
CCGGTCCTGG ACAGAGTGGA TGTGGAGGGG ATGGCCATCC CTACCCATGT GAGCTCCCAG 
TATGTGGCCC TGCTGCAGGG AAGTCATGCT GACCGCTCCC GAGGCAAGAG GTTCAGCCAG 
AACTTTCGAG AGGTGGCAGG CAGGTTCCTG GTGTCAGAGA CCTCCAGTCA CCTGCTGGTG 
TTCGGCATGG AGCAGCGGCT GCCGCCTAAC AGTGAGCTGG TGCAGGCCAT GCTGCGGCTG 
TTCCAGGAGC CCGTGCCCAG AACAGCTCTC CGGAGGCTTG AGAGGCTGCC CCCTCATAGT 
GCCCAAGCTC GGGTCACCAT CGAGTGGCTG AGAGTCCGTG AGGATGGCTC CAACCGCACC 
GCCCTCATCG ATTCCAGGCT CGTGTCCATC TACGAGAGCG GCTGGAAGGT CTTCGATGTG 
ACCGAGGCCG TGAACTTCTG GCAGCAGCTG AGCCGGCCGA GGCAGCCGCT GCTGCTCCAG 
GTGTCGGTGC AGAGGGAGCA TCTGGGACCG GGGACCTGGA GCGCACACAA GTTGGTCCGG 
TTCGCGGCGC AGGGGACGCC GGACGGCAAG GGCGAGCCAC AGCTGGAACT GCACACGCTG 
GACCTCAAGG ACTACGGAGC TCAAGGCAAT TGTGACCCCG AGGCACCAGT GACTGAAGGC 
ACCCGATGCT GTCGCAAGGA GATGTACCTG GACCTGCAGG GGATGAAGTG GGCTGAGAAC 
TGGATCCTAG AACCCCCAGG GTTCCTCATT TATGAATGTG TGGGCAGCTG CCGGCAGCTG 
CCAGAGTCTC TGACCATCGG GTGGCCATTT CTGGGGCCTC GGCAGTGTGT GGCCTCAGAG 
ATGACTTCCC TGCCCATGAT TGTCAGCATA AAGGAGGACG GCAAGACCAG GCCTCAAGTG 
GTCAGCCTGC CCAACATGAG GGTGCAGACG TGTAGTTGTG CCTCGGATGG GGCACTCATA 
CCTAGGGGGA TAGATCTGTA G