Galk1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Galk1
Entrez Gene ID	287835
Full Name	galactokinase 1
Synonyms	Galk,Glk
General protein information	Preferred Names galactokinase 1 Names galactokinase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q32.1
Summary	human homolog catalyzes the conversion of ATP and D-galactose to ADP and D-galactose 1-phosphate in galactose metabolism [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001008282.1	NP_001008283.1	galactokinase

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Galk1	NP_058601.2
Oryza sativa (rice)	Os04g0608100	NP_001053811.1
Homo sapiens (human)	GALK1	NP_000145.1
Rattus norvegicus (Norway rat)	Galk1	NP_001008283.1
Gallus gallus (chicken)	GALK1	XP_415629.3
Danio rerio (zebrafish)	galk1	NP_001006002.1
Arabidopsis thaliana (thale cress)	GalAK	NP_187681.2
	GALK1	XP_002808184.1
Canis lupus familiaris (dog)	GALK1	NP_001003104.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002914	XP_003436307.1
Pan troglodytes (chimpanzee)	GALK1	XP_001138227.2
Bos taurus (cattle)	GALK1	NP_001092851.1
Xenopus tropicalis (tropical clawed frog)	galk1	NP_001072199.1

Gene Ontology
Bibliography

Related articles in PubMed

Defining the membrane proteome of NK cells.
Ghosh D, Lippert D, Krokhin O, Cortens JP, Wilkins JA
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)

Large-scale proteomics and phosphoproteomics of urinary exosomes.
Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal of the American Society of Nephrology : JASN20(2)363-79(2009 Feb)

Biochemical characterization of two GALK1 mutations in patients with galactokinase deficiency.
Sangiuolo F, Magnani M, Stambolian D, Novelli G
Human mutation23(4)396(2004 Apr)

Galactitol and galactonate accumulation in heart and skeletal muscle of mice with deficiency of galactose-1-phosphate uridyltransferase.
Yager C, Ning C, Reynolds R, Leslie N, Segal S
Molecular genetics and metabolism81(2)105-11(2004 Feb)

Sugar recognition by human galactokinase.
Timson DJ, Reece RJ
BMC biochemistry416(2003 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Galk1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Galk1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa11136	NM_001008282.1 Latest version!	Rattus norvegicus galactokinase 1 (Galk1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Galk1 ( NM_001008282.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001008283.1 Rattus norvegicus galactokinase 1 (Galk1), mRNA.

Price & Availability↑

CloneID	ORa11136
Clone ID Related Accession (Same CDS sequence)	NM_001008282.1
Accession Version	NM_001008282.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1179bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-26
Organism	Rattus norvegicus(Norway rat)
Product	galactokinase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC085919.1. On Dec 16, 2004 this sequence version replaced XM_213528.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC085919.1, FQ219172.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01906347, SAMN01906354 [ECO:0000348] ##Evidence-Data-END##

ATGGCTGCTT GGAGACCGCC CCGGGTTGAG GAGCTGCTGG CCGAGGCCCG GCGGGCCTTC 
ATGGAGGAGT TTGGAGCCGA GCCGGAGCTG GCAGTGTCGG CGCCTGGCCG CGTCAACCTC 
ATCGGGGAGC ACACGGATTA CAACCAGGGC CTGGTGCTGC CCATGGCTCT GGAGCTTGTG 
ACGGTGATGG TTGGCAGCCC CCGGACAGAT GGGCTTGTTT CTCTCCTCAC CACTTCCAAA 
GATGCAGACG AGCCCCAGCG ACTGCAGTTC CCACTGCCCT CAGCCCAGTG GTCCTTGGAG 
CCTGGAATCC CACAGTGGGC CAATTATGTC AAGGGAGTGA TTCAGTATTA CCCAGCTTCC 
CCTCTCTGTG GCTTCAGCGC AGTGGTGGTC AGCTCAGTGC CCCTGGGGGG TGGTCTTTCC 
AGCTCAGCTT CTCTGGAAGT GGCCACGTAC GCCTTCCTCC AGCAGCTCTG CCCAGACTCG 
GGGTCAATAG CCGCTCGGGC CCAGGTGTGT CAACGGGCTG AGCACAGCTT CGCAGGGGTG 
CCCTGTGGCA TCATGGACCA ACTCATCGCA CTGCTGGGGC AGAAAGGCTA TGCGCTACTC 
ATTGACTGCA GGTCCCTGGA AACAAGCCTG GTGCCGCTGT CAGACCCTAA GCTGACCGTG 
CTCATCACCA ACTCCAATGT CCGCCATTCC CTGGCCTCCA GCGAGTACCC GATTCGTCGG 
CGCCAGTGTG AAGAGGTGGC CCAGGCCCTG GGCAAGGAGA GCCTTCGGGA GGTGCGGATG 
GAGGAACTTG AGGCGGGCAG GGAGCTGATG AGCAAGGAGG GTTTCCGGCG GGCCCGGCAC 
GTGGTTAGCG AGATCCGGCG AACAGCCCAG GGAGCAGCTG CTCTGAGCCG AGGAGACTAT 
AAGGCCTTTG GACGTCTCAT GGTGGAGAGT CACTATTCCC TCAGAGATGA CTATGAGGTG 
AGCTGCCCTG AGCTGGACCT ATTGGTTGAG GCTGCGCTCT CTGTGCCTGG AGTTTACGGC 
AGTCGCATGA CAGGCGGTGG CTTCGGCGGC TGCACTGTCA CGTTGCTGGA GGCCTCTGTT 
GCCCCCCTTG TCATGCATCA CATACAGGAG CAGTACAGCG GGACAGCCAC CTTCTACCTC 
ACTCAAGCTG CCGATGGAGC CCAGGTGCTG AGCTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001008283.1
CDS	28..1206
Translation	MAAWRPPRVEELLAEARRAFMEEFGAEPELAVSAPGRVNLIGEHTDYNQGLVLPMALELVTVMVGSPRTDGLVSLLTTSKDADEPQRLQFPLPSAQWSLEPGIPQWANYVKGVIQYYPASPLCGFSAVVVSSVPLGGGLSSSASLEVATYAFLQQLCPDSGSIAARAQVCQRAEHSFAGVPCGIMDQLIALLGQKGYALLIDCRSLETSLVPLSDPKLTVLITNSNVRHSLASSEYPIRRRQCEEVAQALGKESLREVRMEELEAGRELMSKEGFRRARHVVSEIRRTAQGAAALSRGDYKAFGRLMVESHYSLRDDYEVSCPELDLLVEAALSVPGVYGSRMTGGGFGGCTVTLLEASVAPLVMHHIQEQYSGTATFYLTQAADGAQVLSL

Target ORF information:

RefSeq Version	NM_001008282.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus galactokinase 1 (Galk1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001008282.1

ORF Insert Sequence:

ATGGCTGCTT GGAGACCGCC CCGGGTTGAG GAGCTGCTGG CCGAGGCCCG GCGGGCCTTC 
ATGGAGGAGT TTGGAGCCGA GCCGGAGCTG GCAGTGTCGG CGCCTGGCCG CGTCAACCTC 
ATCGGGGAGC ACACGGATTA CAACCAGGGC CTGGTGCTGC CCATGGCTCT GGAGCTTGTG 
ACGGTGATGG TTGGCAGCCC CCGGACAGAT GGGCTTGTTT CTCTCCTCAC CACTTCCAAA 
GATGCAGACG AGCCCCAGCG ACTGCAGTTC CCACTGCCCT CAGCCCAGTG GTCCTTGGAG 
CCTGGAATCC CACAGTGGGC CAATTATGTC AAGGGAGTGA TTCAGTATTA CCCAGCTTCC 
CCTCTCTGTG GCTTCAGCGC AGTGGTGGTC AGCTCAGTGC CCCTGGGGGG TGGTCTTTCC 
AGCTCAGCTT CTCTGGAAGT GGCCACGTAC GCCTTCCTCC AGCAGCTCTG CCCAGACTCG 
GGGTCAATAG CCGCTCGGGC CCAGGTGTGT CAACGGGCTG AGCACAGCTT CGCAGGGGTG 
CCCTGTGGCA TCATGGACCA ACTCATCGCA CTGCTGGGGC AGAAAGGCTA TGCGCTACTC 
ATTGACTGCA GGTCCCTGGA AACAAGCCTG GTGCCGCTGT CAGACCCTAA GCTGACCGTG 
CTCATCACCA ACTCCAATGT CCGCCATTCC CTGGCCTCCA GCGAGTACCC GATTCGTCGG 
CGCCAGTGTG AAGAGGTGGC CCAGGCCCTG GGCAAGGAGA GCCTTCGGGA GGTGCGGATG 
GAGGAACTTG AGGCGGGCAG GGAGCTGATG AGCAAGGAGG GTTTCCGGCG GGCCCGGCAC 
GTGGTTAGCG AGATCCGGCG AACAGCCCAG GGAGCAGCTG CTCTGAGCCG AGGAGACTAT 
AAGGCCTTTG GACGTCTCAT GGTGGAGAGT CACTATTCCC TCAGAGATGA CTATGAGGTG 
AGCTGCCCTG AGCTGGACCT ATTGGTTGAG GCTGCGCTCT CTGTGCCTGG AGTTTACGGC 
AGTCGCATGA CAGGCGGTGG CTTCGGCGGC TGCACTGTCA CGTTGCTGGA GGCCTCTGTT 
GCCCCCCTTG TCATGCATCA CATACAGGAG CAGTACAGCG GGACAGCCAC CTTCTACCTC 
ACTCAAGCTG CCGATGGAGC CCAGGTGCTG AGCTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Defining the membrane proteome of NK cells.
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)
Ghosh D,Lippert D,Krokhin O,Cortens JP,Wilkins JA

Large-scale proteomics and phosphoproteomics of urinary exosomes.
Journal of the American Society of Nephrology : JASN20(2)363-79(2009 Feb)
Gonzales PA,Pisitkun T,Hoffert JD,Tchapyjnikov D,Star RA,Kleta R,Wang NS,Knepper MA

Biochemical characterization of two GALK1 mutations in patients with galactokinase deficiency.
Human mutation23(4)396(2004 Apr)
Sangiuolo F,Magnani M,Stambolian D,Novelli G

Galactitol and galactonate accumulation in heart and skeletal muscle of mice with deficiency of galactose-1-phosphate uridyltransferase.
Molecular genetics and metabolism81(2)105-11(2004 Feb)
Yager C,Ning C,Reynolds R,Leslie N,Segal S

Sugar recognition by human galactokinase.
BMC biochemistry416(2003 Nov)
Timson DJ,Reece RJ