Smg8 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Smg8
Entrez Gene ID	287596
Full Name	SMG8 nonsense mediated mRNA decay factor
Synonyms	RGD1306862
General protein information	Preferred Names SMG8 nonsense mediated mRNA decay factor Names protein SMG8
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q26

mRNA and Protein(s)

mRNA	Protein	Name
XM_213430.8	XP_213430.2	protein SMG8
XM_001081151.6	XP_001081151.1	protein SMG8

Pathways from BioSystems

Homologies

Homo sapiens (human)	SMG8	NP_060619.4
Xenopus tropicalis (tropical clawed frog)	smg8	NP_001072291.1
Bos taurus (cattle)	SMG8	NP_001073755.1
Mus musculus (house mouse)	Smg8	NP_077224.1
	C17orf71	XP_001107695.1
Canis lupus familiaris (dog)	SMG8	XP_548236.1
Rattus norvegicus (Norway rat)	Smg8	XP_213430.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010179	XP_319357.4
Pan troglodytes (chimpanzee)	SMG8	XP_523682.1
Gallus gallus (chicken)	SMG8	XP_415875.4
Danio rerio (zebrafish)	smg8	XP_695811.2
Drosophila melanogaster (fruit fly)	CG6729	NP_609449.1

Gene Ontology

The following Smg8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Smg8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa18821	XM_001081151.6 Latest version!	Rattus norvegicus SMG8 nonsense mediated mRNA decay factor (Smg8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa18821	XM_213430.8 Latest version!	Rattus norvegicus SMG8 nonsense mediated mRNA decay factor (Smg8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Smg8 ( XM_213430.8 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_213430.2 Rattus norvegicus SMG8 nonsense mediated mRNA decay factor (Smg8), mRNA.

Price & Availability↑

CloneID	ORa18821
Clone ID Related Accession (Same CDS sequence)	XM_213430.8 , XM_001081151.6
Accession Version	XM_213430.8 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2976bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	protein SMG8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_213430.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGGC CGGTCAGCTT GCGCGAGCTT CTCATGGGGG CGTCAGCCTG GCTGGGCTCT 
GAGAGTCCCG GAGGTCCCTC CGCTGAAGGC GGGGGGAACG CAGCTGCTGC GCCCGAGCCA 
CCGTGGAGGG AGGATGAGAT CTGCGTAGTG GGAATCTTCG GCAAGACGGC TCTGCGCCTA 
AACTCAGAGA AGTTCTCTCT GGTGAACACT GTGTGCGATC GGCAGGTTTT CCCACTCTTT 
CACCATCAGG ATCCTGGGGA TCCAGGGCCT GGAATCAAGA CCAAACCTGT CTCTGTCGGA 
GAGGCCGGGG GAGCCGGGGA CCCGGGGGCT GCTGCCGGGG ATTCACTTCG CGGAGGTATG 
GCTACGGCGG AAGGTAACCG AGCTGAGCCA GGTCCCCAGG ACTTTAGTCT TCTGCAGGCC 
TATTACAATC AGGAAAGCAA AGTTCTGTAT CTTATTCTCA CTTCCATCTG TGACAATTCA 
CAGCTTTTGC GTGCTTGTCG GGCTCTCCAG AGCGGTGAAG CAGGTGGGGG CCTCTCTTTA 
CCACATGCAG AAACACACGA GTTCTGGAAG CATCAGGAGA AGTTGCAATG TCTCAGTCTC 
CTTTACCTCT TCTCTGTCTG TCATATCTTG CTCCTGGTTC ATCCGACTTG TTCCTTTGAC 
ATCACTTATG ATCGAGTATT CAGAGCCCTG GATGGACTGA GACAGAAAGT ACTACCCCTC 
CTCAAAACAG CCATCAAGGA TTGTCCTGTT GGCAAAGATT GGAAGTTAAA TTGCCGACCT 
TGCCCACCTA GACTCCTTTT CCTCTTTCAA CTCAATGGAG CCCTTAAGGT GGAACCCCCT 
CGGAGCCAAG ATACAGCTCA TCCAGATAAA CCCAAGAAGC ACTCTCCCAA GAGAAGACTG 
CAGCATGCCC TGGAGGATCA GATCTATAGA ATCTTCCGCA AGAGTCGAGT CTTGACGAAT 
CAGAGTATCA ACTGCCTCTT TACAGTACCT GCCAACCAAG CTTTTGTGTA CATAGTCCCC 
GGAAGCCAGG AGGAGGACCC AATCGGCATG CTGCTGGACC AACTTAGGAG CCATTGTACC 
GTGAAAGACC CTGAATCTTT GTTGGTGCCC GCACCCCTTT CTGGACCTAG GCGGTACCAA 
GCGATGAGAC AGCACAGCCG GCAGCAGCTT TCCTTCCACA TTGATAGTAG CACCTCGAGT 
TCTTCAGGCC AGCTAGTGGA TTTCACTCTT CGAGAATTCC TGTGGCAGCA CGTGGAGCTG 
GTCCTGAGCA AAAAAGGTTT TGATGACAGT GTGGGCAGGA ACCCACAGCC TTCCCATTTT 
GAACTGCCTA CTTACCAGAA GTGGATCTCC GCAGCTGCAA AGCTGTATGA GGTGGCTATC 
GATGGGAAGG AAGAGGACTT GGGCTCACCC ACTGGGGAGC TAACATCTAA GATTTTAAGC 
AGTATTAAAG TCTTAGAAGG GTTTTTGGAT ATAGACACAA AGTTCTCAGA AAACCGGTGC 
CAAAAAGCTT TGCCCATGGC TCACAGTGCC TACCAGTCAA ACTTGCCGCA CAATTACACC 
ATGACAGTCC ATAAGAATCA GCTCGCTCAG GCTCTTCGGG TATACAGTCA ACATGCTCGA 
GGTCCAGCCT TTCACAAATA CGCCATGCAG TTGCACGAAG ACTGCTACAA ATTTTGGAGC 
AATGGCCATC AGCTCTGTGA AGAGAGGAGT TTGACCGACC AACACTGTGT ACACAAATTT 
CACTCCTTAC CTAAATCAGG AGAAAAACCA GAGGCAGATA GGAACCCTCC TGTGCTCTAC 
CACAACAGCC GAGCCCGCTC CACTGGAGCC TGCAACTGTG GAAGGAAGCA GGCCCCTCGA 
GACGACCCCT TCGATATCAA GGCTGCTAAC TATGACTTTT ACCAGCTTCT GGAAGAAAAA 
TGTTGTGGGA AATTGGATCA TATCAATTTT CCTGTATTTG AGCCAAGTAC TCCAGACCCT 
GCTCCGGCTA AAAATGAGCC CTGTCCCACC CCAGCAGACT CGGATGCCGA GAAACTGAAA 
GAGAAAGAAC CTCAAACCCA AGGAGAGGGC ACAAGCCTCA GTCTGGCTTT GAGCCTGGGC 
CAGTCAACAG ACAGCCTAGG CACCTACCCA GCTGATCCGC AGGCAGGAGG AGACAACCCA 
GACGTGCATG GGCAAGGAGA AGTAAAATCT GAGAAGAGAC CAAACTTGGT TGACCGGCAG 
GCATCCACAG TTGAATATCT CCCAGGCATG CTACATTCAA ATTGCCCAAA AGGCCTCTTA 
CCCAAATTCT CCAGTTGGTC TTTGGTTAAA CTAGGCCCAG CCAAGTCTTA CAACTTTCAT 
ACAGGTTTGG ACCAACAGGG CTTTGTCCCA GGAACAAACT ATCTTATGCC TTGGGACATT 
GTCATCAGGA CTAGAGCTGA AGACGAAGGA GACTTAGACA CGAACTCCTG GCCTGCTCCA 
AATAAAGCTA TTCCTGGAAA GAGAAGTGCG GTTGTGATGG GCAGAGGAAG GCGGAGGGAT 
GACATAGCTC GAGCTTTTGT TGGCTTTGAG TATGAAGACT CTCGAGGTCG GAGATTCATG 
TGCTCAGGGC CTGACAAAGT AATGAAAGTG ATGGGAAGTG GACCGAAGGA GTCGGCTTTA 
AAAGCCCTCA ACAGCGACAT GCCCCTGTAC ATCCTGTCCT CGTCGCAGGG CAGAGGGCTG 
AAGCCACACT ATGCTCAACT TATGAGGCTT TTTGTTGTAG TTCCTGACGC TCCTTTGCAG 
ATAATACTGA TGCCTCAGGT TCAACCAGGC CCACCACCGT GTCCAGTATT TTACCCAGAA 
AAACAAGAGC TCACTCTCCC ACCTGATGGC CTTTGGGTCT TGAGATTTCC TTATGCATAT 
GTGACTGAGA GAGGACCTTG TTTCCCTCCT AAGGAGAACG TGCAGCTCAT GAGCTACAAG 
GTGCTCCGAG GAGTCCTCAG AGCAGTTACA CAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_213430.2
CDS	606..3581
Translation	MAGPVSLRELLMGASAWLGSESPGGPSAEGGGNAAAAPEPPWREDEICVVGIFGKTALRLNSEKFSLVNTVCDRQVFPLFHHQDPGDPGPGIKTKPVSVGEAGGAGDPGAAAGDSLRGGMATAEGNRAEPGPQDFSLLQAYYNQESKVLYLILTSICDNSQLLRACRALQSGEAGGGLSLPHAETHEFWKHQEKLQCLSLLYLFSVCHILLLVHPTCSFDITYDRVFRALDGLRQKVLPLLKTAIKDCPVGKDWKLNCRPCPPRLLFLFQLNGALKVEPPRSQDTAHPDKPKKHSPKRRLQHALEDQIYRIFRKSRVLTNQSINCLFTVPANQAFVYIVPGSQEEDPIGMLLDQLRSHCTVKDPESLLVPAPLSGPRRYQAMRQHSRQQLSFHIDSSTSSSSGQLVDFTLREFLWQHVELVLSKKGFDDSVGRNPQPSHFELPTYQKWISAAAKLYEVAIDGKEEDLGSPTGELTSKILSSIKVLEGFLDIDTKFSENRCQKALPMAHSAYQSNLPHNYTMTVHKNQLAQALRVYSQHARGPAFHKYAMQLHEDCYKFWSNGHQLCEERSLTDQHCVHKFHSLPKSGEKPEADRNPPVLYHNSRARSTGACNCGRKQAPRDDPFDIKAANYDFYQLLEEKCCGKLDHINFPVFEPSTPDPAPAKNEPCPTPADSDAEKLKEKEPQTQGEGTSLSLALSLGQSTDSLGTYPADPQAGGDNPDVHGQGEVKSEKRPNLVDRQASTVEYLPGMLHSNCPKGLLPKFSSWSLVKLGPAKSYNFHTGLDQQGFVPGTNYLMPWDIVIRTRAEDEGDLDTNSWPAPNKAIPGKRSAVVMGRGRRRDDIARAFVGFEYEDSRGRRFMCSGPDKVMKVMGSGPKESALKALNSDMPLYILSSSQGRGLKPHYAQLMRLFVVVPDAPLQIILMPQVQPGPPPCPVFYPEKQELTLPPDGLWVLRFPYAYVTERGPCFPPKENVQLMSYKVLRGVLRAVTQ

Target ORF information:

RefSeq Version	XM_213430.8
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus SMG8 nonsense mediated mRNA decay factor (Smg8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_213430.8

ORF Insert Sequence:

ATGGCGGGGC CGGTCAGCTT GCGCGAGCTT CTCATGGGGG CGTCAGCCTG GCTGGGCTCT 
GAGAGTCCCG GAGGTCCCTC CGCTGAAGGC GGGGGGAACG CAGCTGCTGC GCCCGAGCCA 
CCGTGGAGGG AGGATGAGAT CTGCGTAGTG GGAATCTTCG GCAAGACGGC TCTGCGCCTA 
AACTCAGAGA AGTTCTCTCT GGTGAACACT GTGTGCGATC GGCAGGTTTT CCCACTCTTT 
CACCATCAGG ATCCTGGGGA TCCAGGGCCT GGAATCAAGA CCAAACCTGT CTCTGTCGGA 
GAGGCCGGGG GAGCCGGGGA CCCGGGGGCT GCTGCCGGGG ATTCACTTCG CGGAGGTATG 
GCTACGGCGG AAGGTAACCG AGCTGAGCCA GGTCCCCAGG ACTTTAGTCT TCTGCAGGCC 
TATTACAATC AGGAAAGCAA AGTTCTGTAT CTTATTCTCA CTTCCATCTG TGACAATTCA 
CAGCTTTTGC GTGCTTGTCG GGCTCTCCAG AGCGGTGAAG CAGGTGGGGG CCTCTCTTTA 
CCACATGCAG AAACACACGA GTTCTGGAAG CATCAGGAGA AGTTGCAATG TCTCAGTCTC 
CTTTACCTCT TCTCTGTCTG TCATATCTTG CTCCTGGTTC ATCCGACTTG TTCCTTTGAC 
ATCACTTATG ATCGAGTATT CAGAGCCCTG GATGGACTGA GACAGAAAGT ACTACCCCTC 
CTCAAAACAG CCATCAAGGA TTGTCCTGTT GGCAAAGATT GGAAGTTAAA TTGCCGACCT 
TGCCCACCTA GACTCCTTTT CCTCTTTCAA CTCAATGGAG CCCTTAAGGT GGAACCCCCT 
CGGAGCCAAG ATACAGCTCA TCCAGATAAA CCCAAGAAGC ACTCTCCCAA GAGAAGACTG 
CAGCATGCCC TGGAGGATCA GATCTATAGA ATCTTCCGCA AGAGTCGAGT CTTGACGAAT 
CAGAGTATCA ACTGCCTCTT TACAGTACCT GCCAACCAAG CTTTTGTGTA CATAGTCCCC 
GGAAGCCAGG AGGAGGACCC AATCGGCATG CTGCTGGACC AACTTAGGAG CCATTGTACC 
GTGAAAGACC CTGAATCTTT GTTGGTGCCC GCACCCCTTT CTGGACCTAG GCGGTACCAA 
GCGATGAGAC AGCACAGCCG GCAGCAGCTT TCCTTCCACA TTGATAGTAG CACCTCGAGT 
TCTTCAGGCC AGCTAGTGGA TTTCACTCTT CGAGAATTCC TGTGGCAGCA CGTGGAGCTG 
GTCCTGAGCA AAAAAGGTTT TGATGACAGT GTGGGCAGGA ACCCACAGCC TTCCCATTTT 
GAACTGCCTA CTTACCAGAA GTGGATCTCC GCAGCTGCAA AGCTGTATGA GGTGGCTATC 
GATGGGAAGG AAGAGGACTT GGGCTCACCC ACTGGGGAGC TAACATCTAA GATTTTAAGC 
AGTATTAAAG TCTTAGAAGG GTTTTTGGAT ATAGACACAA AGTTCTCAGA AAACCGGTGC 
CAAAAAGCTT TGCCCATGGC TCACAGTGCC TACCAGTCAA ACTTGCCGCA CAATTACACC 
ATGACAGTCC ATAAGAATCA GCTCGCTCAG GCTCTTCGGG TATACAGTCA ACATGCTCGA 
GGTCCAGCCT TTCACAAATA CGCCATGCAG TTGCACGAAG ACTGCTACAA ATTTTGGAGC 
AATGGCCATC AGCTCTGTGA AGAGAGGAGT TTGACCGACC AACACTGTGT ACACAAATTT 
CACTCCTTAC CTAAATCAGG AGAAAAACCA GAGGCAGATA GGAACCCTCC TGTGCTCTAC 
CACAACAGCC GAGCCCGCTC CACTGGAGCC TGCAACTGTG GAAGGAAGCA GGCCCCTCGA 
GACGACCCCT TCGATATCAA GGCTGCTAAC TATGACTTTT ACCAGCTTCT GGAAGAAAAA 
TGTTGTGGGA AATTGGATCA TATCAATTTT CCTGTATTTG AGCCAAGTAC TCCAGACCCT 
GCTCCGGCTA AAAATGAGCC CTGTCCCACC CCAGCAGACT CGGATGCCGA GAAACTGAAA 
GAGAAAGAAC CTCAAACCCA AGGAGAGGGC ACAAGCCTCA GTCTGGCTTT GAGCCTGGGC 
CAGTCAACAG ACAGCCTAGG CACCTACCCA GCTGATCCGC AGGCAGGAGG AGACAACCCA 
GACGTGCATG GGCAAGGAGA AGTAAAATCT GAGAAGAGAC CAAACTTGGT TGACCGGCAG 
GCATCCACAG TTGAATATCT CCCAGGCATG CTACATTCAA ATTGCCCAAA AGGCCTCTTA 
CCCAAATTCT CCAGTTGGTC TTTGGTTAAA CTAGGCCCAG CCAAGTCTTA CAACTTTCAT 
ACAGGTTTGG ACCAACAGGG CTTTGTCCCA GGAACAAACT ATCTTATGCC TTGGGACATT 
GTCATCAGGA CTAGAGCTGA AGACGAAGGA GACTTAGACA CGAACTCCTG GCCTGCTCCA 
AATAAAGCTA TTCCTGGAAA GAGAAGTGCG GTTGTGATGG GCAGAGGAAG GCGGAGGGAT 
GACATAGCTC GAGCTTTTGT TGGCTTTGAG TATGAAGACT CTCGAGGTCG GAGATTCATG 
TGCTCAGGGC CTGACAAAGT AATGAAAGTG ATGGGAAGTG GACCGAAGGA GTCGGCTTTA 
AAAGCCCTCA ACAGCGACAT GCCCCTGTAC ATCCTGTCCT CGTCGCAGGG CAGAGGGCTG 
AAGCCACACT ATGCTCAACT TATGAGGCTT TTTGTTGTAG TTCCTGACGC TCCTTTGCAG 
ATAATACTGA TGCCTCAGGT TCAACCAGGC CCACCACCGT GTCCAGTATT TTACCCAGAA 
AAACAAGAGC TCACTCTCCC ACCTGATGGC CTTTGGGTCT TGAGATTTCC TTATGCATAT 
GTGACTGAGA GAGGACCTTG TTTCCCTCCT AAGGAGAACG TGCAGCTCAT GAGCTACAAG 
GTGCTCCGAG GAGTCCTCAG AGCAGTTACA CAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Smg8 ( XM_001081151.6 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_001081151.1 Rattus norvegicus SMG8 nonsense mediated mRNA decay factor (Smg8), mRNA.

Price & Availability↑

CloneID	ORa18821
Clone ID Related Accession (Same CDS sequence)	XM_213430.8 , XM_001081151.6
Accession Version	XM_001081151.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2976bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	protein SMG8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000078.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_001081151.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGGC CGGTCAGCTT GCGCGAGCTT CTCATGGGGG CGTCAGCCTG GCTGGGCTCT 
GAGAGTCCCG GAGGTCCCTC CGCTGAAGGC GGGGGGAACG CAGCTGCTGC GCCCGAGCCA 
CCGTGGAGGG AGGATGAGAT CTGCGTAGTG GGAATCTTCG GCAAGACGGC TCTGCGCCTA 
AACTCAGAGA AGTTCTCTCT GGTGAACACT GTGTGCGATC GGCAGGTTTT CCCACTCTTT 
CACCATCAGG ATCCTGGGGA TCCAGGGCCT GGAATCAAGA CCAAACCTGT CTCTGTCGGA 
GAGGCCGGGG GAGCCGGGGA CCCGGGGGCT GCTGCCGGGG ATTCACTTCG CGGAGGTATG 
GCTACGGCGG AAGGTAACCG AGCTGAGCCA GGTCCCCAGG ACTTTAGTCT TCTGCAGGCC 
TATTACAATC AGGAAAGCAA AGTTCTGTAT CTTATTCTCA CTTCCATCTG TGACAATTCA 
CAGCTTTTGC GTGCTTGTCG GGCTCTCCAG AGCGGTGAAG CAGGTGGGGG CCTCTCTTTA 
CCACATGCAG AAACACACGA GTTCTGGAAG CATCAGGAGA AGTTGCAATG TCTCAGTCTC 
CTTTACCTCT TCTCTGTCTG TCATATCTTG CTCCTGGTTC ATCCGACTTG TTCCTTTGAC 
ATCACTTATG ATCGAGTATT CAGAGCCCTG GATGGACTGA GACAGAAAGT ACTACCCCTC 
CTCAAAACAG CCATCAAGGA TTGTCCTGTT GGCAAAGATT GGAAGTTAAA TTGCCGACCT 
TGCCCACCTA GACTCCTTTT CCTCTTTCAA CTCAATGGAG CCCTTAAGGT GGAACCCCCT 
CGGAGCCAAG ATACAGCTCA TCCAGATAAA CCCAAGAAGC ACTCTCCCAA GAGAAGACTG 
CAGCATGCCC TGGAGGATCA GATCTATAGA ATCTTCCGCA AGAGTCGAGT CTTGACGAAT 
CAGAGTATCA ACTGCCTCTT TACAGTACCT GCCAACCAAG CTTTTGTGTA CATAGTCCCC 
GGAAGCCAGG AGGAGGACCC AATCGGCATG CTGCTGGACC AACTTAGGAG CCATTGTACC 
GTGAAAGACC CTGAATCTTT GTTGGTGCCC GCACCCCTTT CTGGACCTAG GCGGTACCAA 
GCGATGAGAC AGCACAGCCG GCAGCAGCTT TCCTTCCACA TTGATAGTAG CACCTCGAGT 
TCTTCAGGCC AGCTAGTGGA TTTCACTCTT CGAGAATTCC TGTGGCAGCA CGTGGAGCTG 
GTCCTGAGCA AAAAAGGTTT TGATGACAGT GTGGGCAGGA ACCCACAGCC TTCCCATTTT 
GAACTGCCTA CTTACCAGAA GTGGATCTCC GCAGCTGCAA AGCTGTATGA GGTGGCTATC 
GATGGGAAGG AAGAGGACTT GGGCTCACCC ACTGGGGAGC TAACATCTAA GATTTTAAGC 
AGTATTAAAG TCTTAGAAGG GTTTTTGGAT ATAGACACAA AGTTCTCAGA AAACCGGTGC 
CAAAAAGCTT TGCCCATGGC TCACAGTGCC TACCAGTCAA ACTTGCCGCA CAATTACACC 
ATGACAGTCC ATAAGAATCA GCTCGCTCAG GCTCTTCGGG TATACAGTCA ACATGCTCGA 
GGTCCAGCCT TTCACAAATA CGCCATGCAG TTGCACGAAG ACTGCTACAA ATTTTGGAGC 
AATGGCCATC AGCTCTGTGA AGAGAGGAGT TTGACCGACC AACACTGTGT ACACAAATTT 
CACTCCTTAC CTAAATCAGG AGAAAAACCA GAGGCAGATA GGAACCCTCC TGTGCTCTAC 
CACAACAGCC GAGCCCGCTC CACTGGAGCC TGCAACTGTG GAAGGAAGCA GGCCCCTCGA 
GACGACCCCT TCGATATCAA GGCTGCTAAC TATGACTTTT ACCAGCTTCT GGAAGAAAAA 
TGTTGTGGGA AATTGGATCA TATCAATTTT CCTGTATTTG AGCCAAGTAC TCCAGACCCT 
GCTCCGGCTA AAAATGAGCC CTGTCCCACC CCAGCAGACT CGGATGCCGA GAAACTGAAA 
GAGAAAGAAC CTCAAACCCA AGGAGAGGGC ACAAGCCTCA GTCTGGCTTT GAGCCTGGGC 
CAGTCAACAG ACAGCCTAGG CACCTACCCA GCTGATCCGC AGGCAGGAGG AGACAACCCA 
GACGTGCATG GGCAAGGAGA AGTAAAATCT GAGAAGAGAC CAAACTTGGT TGACCGGCAG 
GCATCCACAG TTGAATATCT CCCAGGCATG CTACATTCAA ATTGCCCAAA AGGCCTCTTA 
CCCAAATTCT CCAGTTGGTC TTTGGTTAAA CTAGGCCCAG CCAAGTCTTA CAACTTTCAT 
ACAGGTTTGG ACCAACAGGG CTTTGTCCCA GGAACAAACT ATCTTATGCC TTGGGACATT 
GTCATCAGGA CTAGAGCTGA AGACGAAGGA GACTTAGACA CGAACTCCTG GCCTGCTCCA 
AATAAAGCTA TTCCTGGAAA GAGAAGTGCG GTTGTGATGG GCAGAGGAAG GCGGAGGGAT 
GACATAGCTC GAGCTTTTGT TGGCTTTGAG TATGAAGACT CTCGAGGTCG GAGATTCATG 
TGCTCAGGGC CTGACAAAGT AATGAAAGTG ATGGGAAGTG GACCGAAGGA GTCGGCTTTA 
AAAGCCCTCA ACAGCGACAT GCCCCTGTAC ATCCTGTCCT CGTCGCAGGG CAGAGGGCTG 
AAGCCACACT ATGCTCAACT TATGAGGCTT TTTGTTGTAG TTCCTGACGC TCCTTTGCAG 
ATAATACTGA TGCCTCAGGT TCAACCAGGC CCACCACCGT GTCCAGTATT TTACCCAGAA 
AAACAAGAGC TCACTCTCCC ACCTGATGGC CTTTGGGTCT TGAGATTTCC TTATGCATAT 
GTGACTGAGA GAGGACCTTG TTTCCCTCCT AAGGAGAACG TGCAGCTCAT GAGCTACAAG 
GTGCTCCGAG GAGTCCTCAG AGCAGTTACA CAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001081151.1
CDS	606..3581
Translation	MAGPVSLRELLMGASAWLGSESPGGPSAEGGGNAAAAPEPPWREDEICVVGIFGKTALRLNSEKFSLVNTVCDRQVFPLFHHQDPGDPGPGIKTKPVSVGEAGGAGDPGAAAGDSLRGGMATAEGNRAEPGPQDFSLLQAYYNQESKVLYLILTSICDNSQLLRACRALQSGEAGGGLSLPHAETHEFWKHQEKLQCLSLLYLFSVCHILLLVHPTCSFDITYDRVFRALDGLRQKVLPLLKTAIKDCPVGKDWKLNCRPCPPRLLFLFQLNGALKVEPPRSQDTAHPDKPKKHSPKRRLQHALEDQIYRIFRKSRVLTNQSINCLFTVPANQAFVYIVPGSQEEDPIGMLLDQLRSHCTVKDPESLLVPAPLSGPRRYQAMRQHSRQQLSFHIDSSTSSSSGQLVDFTLREFLWQHVELVLSKKGFDDSVGRNPQPSHFELPTYQKWISAAAKLYEVAIDGKEEDLGSPTGELTSKILSSIKVLEGFLDIDTKFSENRCQKALPMAHSAYQSNLPHNYTMTVHKNQLAQALRVYSQHARGPAFHKYAMQLHEDCYKFWSNGHQLCEERSLTDQHCVHKFHSLPKSGEKPEADRNPPVLYHNSRARSTGACNCGRKQAPRDDPFDIKAANYDFYQLLEEKCCGKLDHINFPVFEPSTPDPAPAKNEPCPTPADSDAEKLKEKEPQTQGEGTSLSLALSLGQSTDSLGTYPADPQAGGDNPDVHGQGEVKSEKRPNLVDRQASTVEYLPGMLHSNCPKGLLPKFSSWSLVKLGPAKSYNFHTGLDQQGFVPGTNYLMPWDIVIRTRAEDEGDLDTNSWPAPNKAIPGKRSAVVMGRGRRRDDIARAFVGFEYEDSRGRRFMCSGPDKVMKVMGSGPKESALKALNSDMPLYILSSSQGRGLKPHYAQLMRLFVVVPDAPLQIILMPQVQPGPPPCPVFYPEKQELTLPPDGLWVLRFPYAYVTERGPCFPPKENVQLMSYKVLRGVLRAVTQ

Target ORF information:

RefSeq Version	XM_001081151.6
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus SMG8 nonsense mediated mRNA decay factor (Smg8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001081151.6

ORF Insert Sequence:

ATGGCGGGGC CGGTCAGCTT GCGCGAGCTT CTCATGGGGG CGTCAGCCTG GCTGGGCTCT 
GAGAGTCCCG GAGGTCCCTC CGCTGAAGGC GGGGGGAACG CAGCTGCTGC GCCCGAGCCA 
CCGTGGAGGG AGGATGAGAT CTGCGTAGTG GGAATCTTCG GCAAGACGGC TCTGCGCCTA 
AACTCAGAGA AGTTCTCTCT GGTGAACACT GTGTGCGATC GGCAGGTTTT CCCACTCTTT 
CACCATCAGG ATCCTGGGGA TCCAGGGCCT GGAATCAAGA CCAAACCTGT CTCTGTCGGA 
GAGGCCGGGG GAGCCGGGGA CCCGGGGGCT GCTGCCGGGG ATTCACTTCG CGGAGGTATG 
GCTACGGCGG AAGGTAACCG AGCTGAGCCA GGTCCCCAGG ACTTTAGTCT TCTGCAGGCC 
TATTACAATC AGGAAAGCAA AGTTCTGTAT CTTATTCTCA CTTCCATCTG TGACAATTCA 
CAGCTTTTGC GTGCTTGTCG GGCTCTCCAG AGCGGTGAAG CAGGTGGGGG CCTCTCTTTA 
CCACATGCAG AAACACACGA GTTCTGGAAG CATCAGGAGA AGTTGCAATG TCTCAGTCTC 
CTTTACCTCT TCTCTGTCTG TCATATCTTG CTCCTGGTTC ATCCGACTTG TTCCTTTGAC 
ATCACTTATG ATCGAGTATT CAGAGCCCTG GATGGACTGA GACAGAAAGT ACTACCCCTC 
CTCAAAACAG CCATCAAGGA TTGTCCTGTT GGCAAAGATT GGAAGTTAAA TTGCCGACCT 
TGCCCACCTA GACTCCTTTT CCTCTTTCAA CTCAATGGAG CCCTTAAGGT GGAACCCCCT 
CGGAGCCAAG ATACAGCTCA TCCAGATAAA CCCAAGAAGC ACTCTCCCAA GAGAAGACTG 
CAGCATGCCC TGGAGGATCA GATCTATAGA ATCTTCCGCA AGAGTCGAGT CTTGACGAAT 
CAGAGTATCA ACTGCCTCTT TACAGTACCT GCCAACCAAG CTTTTGTGTA CATAGTCCCC 
GGAAGCCAGG AGGAGGACCC AATCGGCATG CTGCTGGACC AACTTAGGAG CCATTGTACC 
GTGAAAGACC CTGAATCTTT GTTGGTGCCC GCACCCCTTT CTGGACCTAG GCGGTACCAA 
GCGATGAGAC AGCACAGCCG GCAGCAGCTT TCCTTCCACA TTGATAGTAG CACCTCGAGT 
TCTTCAGGCC AGCTAGTGGA TTTCACTCTT CGAGAATTCC TGTGGCAGCA CGTGGAGCTG 
GTCCTGAGCA AAAAAGGTTT TGATGACAGT GTGGGCAGGA ACCCACAGCC TTCCCATTTT 
GAACTGCCTA CTTACCAGAA GTGGATCTCC GCAGCTGCAA AGCTGTATGA GGTGGCTATC 
GATGGGAAGG AAGAGGACTT GGGCTCACCC ACTGGGGAGC TAACATCTAA GATTTTAAGC 
AGTATTAAAG TCTTAGAAGG GTTTTTGGAT ATAGACACAA AGTTCTCAGA AAACCGGTGC 
CAAAAAGCTT TGCCCATGGC TCACAGTGCC TACCAGTCAA ACTTGCCGCA CAATTACACC 
ATGACAGTCC ATAAGAATCA GCTCGCTCAG GCTCTTCGGG TATACAGTCA ACATGCTCGA 
GGTCCAGCCT TTCACAAATA CGCCATGCAG TTGCACGAAG ACTGCTACAA ATTTTGGAGC 
AATGGCCATC AGCTCTGTGA AGAGAGGAGT TTGACCGACC AACACTGTGT ACACAAATTT 
CACTCCTTAC CTAAATCAGG AGAAAAACCA GAGGCAGATA GGAACCCTCC TGTGCTCTAC 
CACAACAGCC GAGCCCGCTC CACTGGAGCC TGCAACTGTG GAAGGAAGCA GGCCCCTCGA 
GACGACCCCT TCGATATCAA GGCTGCTAAC TATGACTTTT ACCAGCTTCT GGAAGAAAAA 
TGTTGTGGGA AATTGGATCA TATCAATTTT CCTGTATTTG AGCCAAGTAC TCCAGACCCT 
GCTCCGGCTA AAAATGAGCC CTGTCCCACC CCAGCAGACT CGGATGCCGA GAAACTGAAA 
GAGAAAGAAC CTCAAACCCA AGGAGAGGGC ACAAGCCTCA GTCTGGCTTT GAGCCTGGGC 
CAGTCAACAG ACAGCCTAGG CACCTACCCA GCTGATCCGC AGGCAGGAGG AGACAACCCA 
GACGTGCATG GGCAAGGAGA AGTAAAATCT GAGAAGAGAC CAAACTTGGT TGACCGGCAG 
GCATCCACAG TTGAATATCT CCCAGGCATG CTACATTCAA ATTGCCCAAA AGGCCTCTTA 
CCCAAATTCT CCAGTTGGTC TTTGGTTAAA CTAGGCCCAG CCAAGTCTTA CAACTTTCAT 
ACAGGTTTGG ACCAACAGGG CTTTGTCCCA GGAACAAACT ATCTTATGCC TTGGGACATT 
GTCATCAGGA CTAGAGCTGA AGACGAAGGA GACTTAGACA CGAACTCCTG GCCTGCTCCA 
AATAAAGCTA TTCCTGGAAA GAGAAGTGCG GTTGTGATGG GCAGAGGAAG GCGGAGGGAT 
GACATAGCTC GAGCTTTTGT TGGCTTTGAG TATGAAGACT CTCGAGGTCG GAGATTCATG 
TGCTCAGGGC CTGACAAAGT AATGAAAGTG ATGGGAAGTG GACCGAAGGA GTCGGCTTTA 
AAAGCCCTCA ACAGCGACAT GCCCCTGTAC ATCCTGTCCT CGTCGCAGGG CAGAGGGCTG 
AAGCCACACT ATGCTCAACT TATGAGGCTT TTTGTTGTAG TTCCTGACGC TCCTTTGCAG 
ATAATACTGA TGCCTCAGGT TCAACCAGGC CCACCACCGT GTCCAGTATT TTACCCAGAA 
AAACAAGAGC TCACTCTCCC ACCTGATGGC CTTTGGGTCT TGAGATTTCC TTATGCATAT 
GTGACTGAGA GAGGACCTTG TTTCCCTCCT AAGGAGAACG TGCAGCTCAT GAGCTACAAG 
GTGCTCCGAG GAGTCCTCAG AGCAGTTACA CAATGA