Rflnb cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rflnb
Entrez Gene ID	287534
Full Name	refilin B
Synonyms	Fam101b,RGD1359691
General protein information	Preferred Names refilin B Names refilin-B family with sequence similarity 101, member B filamin-interacting protein FAM101B protein FAM101B refilinB regulator of filamin protein B
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q24

mRNA and Protein(s)

mRNA	Protein	Name
NM_001007611.1	NP_001007612.1	refilin-B

Pathways from BioSystems

Homologies

Homo sapiens (human)	FAM101B	NP_874364.1
	LOC699332	XP_001083814.2
Canis lupus familiaris (dog)	FAM101B	XP_853944.1
Mus musculus (house mouse)	Fam101b	NP_083934.1
Rattus norvegicus (Norway rat)	Fam101b	NP_001007612.1
Gallus gallus (chicken)	FAM101B	XP_415867.3
Bos taurus (cattle)	FAM101B	XP_002695757.2
Pan troglodytes (chimpanzee)	FAM101B	XP_511242.3
Danio rerio (zebrafish)	fam101b	XP_002664671.1
Xenopus tropicalis (tropical clawed frog)	fam101b	NP_001008059.1

Gene Ontology
Bibliography

Related articles in PubMed

Filamin-interacting proteins, Cfm1 and Cfm2, are essential for the formation of cartilaginous skeletal elements.
Mizuhashi K, Kanamoto T, Moriishi T, Muranishi Y, Miyazaki T, Terada K, Omori Y, Ito M, Komori T, Furukawa T
Human molecular genetics23(11)2953-67(2014 Jun)

RefilinB (FAM101B) targets filamin A to organize perinuclear actin networks and regulates nuclear shape.
Gay O, Gilquin B, Nakamura F, Jenkins ZA, McCartney R, Krakow D, Deshiere A, Assard N, Hartwig JH, Robertson SP, Baudier J
Proceedings of the National Academy of Sciences of the United States of America108(28)11464-9(2011 Jul)

cfm is a novel gene uniquely expressed in developing forebrain and midbrain, but its null mutant exhibits no obvious phenotype.
Hirano M, Murata T, Furushima K, Kiyonari H, Nakamura M, Suda Y, Aizawa S
Gene expression patterns : GEP5(3)439-44(2005 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Rflnb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rflnb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa11121	NM_001007611.1 Latest version!	Rattus norvegicus refilin B (Rflnb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rflnb ( NM_001007611.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001007612.1 Rattus norvegicus refilin B (Rflnb), mRNA.

Price & Availability↑

CloneID	ORa11121
Clone ID Related Accession (Same CDS sequence)	NM_001007611.1
Accession Version	NM_001007611.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 651bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-16
Organism	Rattus norvegicus(Norway rat)
Product	refilin-B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC079330.1. On Nov 29, 2004 this sequence version replaced XM_213390.1. ##Evidence-Data-START## Transcript exon combination :: BC079330.1, EX491010.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGTGGGCC GACTGAGCCT GCAGGATGTC CCCGAGCTCG TGGACACGAA GAAAAAAGGA 
GACGGCGTCC TGGACAGCCC GGACTCGGGG CTGCCCCCAA GCCCTAGCCC CAGCCACTGG 
GGGCTCGCAG CGGCGACGGG CGGCGGTGGG GAGCGCGCTC CGGTCGCAGG GACGCTGGAG 
CCCGATGCGA CGGTCACCTC CGTAGTCCCG AACCCAGCAT CTCTGAGCCA CTCACTGGCT 
GGCATCTGCT CACCACGACT CTGTCCCCTG TCCTTCGGCG AAGGAGTCGA GTTTGACCCC 
TTACCACCAA AGGAAATAAA GTATACCTCC TCAGTCAAGT ACGACTCTGA GCGGCATTTC 
ATCGATGATG TGCAGATGCC CCTGGGCCTG GTGGTGGCTT CCTGCAGCCA GACAGTCACC 
TGTATCCCCA ATTGCACTTG GCGAAACTAT AAGGCTGAAG TGCGCTTCGA GCCTCGCCAC 
AAGCCTGCGC GCTTCCTCAG CACCACCATC ATCTACCCCA AGTACCCGAA GACCGTCTAC 
ACCACCACTC TGGATTACAA CTGCCACAAG AAACTGAGGA GGTTTCTATC CAGTGTGGAG 
CTGGAGGCCA CGGAGTTCCT AGGTAGCGAT GGGCTGTTGG ATGAATGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001007612.1
CDS	121..771
Translation	MVGRLSLQDVPELVDTKKKGDGVLDSPDSGLPPSPSPSHWGLAAATGGGGERAPVAGTLEPDATVTSVVPNPASLSHSLAGICSPRLCPLSFGEGVEFDPLPPKEIKYTSSVKYDSERHFIDDVQMPLGLVVASCSQTVTCIPNCTWRNYKAEVRFEPRHKPARFLSTTIIYPKYPKTVYTTTLDYNCHKKLRRFLSSVELEATEFLGSDGLLDEC

Target ORF information:

RefSeq Version	NM_001007611.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus refilin B (Rflnb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001007611.1

ORF Insert Sequence:

ATGGTGGGCC GACTGAGCCT GCAGGATGTC CCCGAGCTCG TGGACACGAA GAAAAAAGGA 
GACGGCGTCC TGGACAGCCC GGACTCGGGG CTGCCCCCAA GCCCTAGCCC CAGCCACTGG 
GGGCTCGCAG CGGCGACGGG CGGCGGTGGG GAGCGCGCTC CGGTCGCAGG GACGCTGGAG 
CCCGATGCGA CGGTCACCTC CGTAGTCCCG AACCCAGCAT CTCTGAGCCA CTCACTGGCT 
GGCATCTGCT CACCACGACT CTGTCCCCTG TCCTTCGGCG AAGGAGTCGA GTTTGACCCC 
TTACCACCAA AGGAAATAAA GTATACCTCC TCAGTCAAGT ACGACTCTGA GCGGCATTTC 
ATCGATGATG TGCAGATGCC CCTGGGCCTG GTGGTGGCTT CCTGCAGCCA GACAGTCACC 
TGTATCCCCA ATTGCACTTG GCGAAACTAT AAGGCTGAAG TGCGCTTCGA GCCTCGCCAC 
AAGCCTGCGC GCTTCCTCAG CACCACCATC ATCTACCCCA AGTACCCGAA GACCGTCTAC 
ACCACCACTC TGGATTACAA CTGCCACAAG AAACTGAGGA GGTTTCTATC CAGTGTGGAG 
CTGGAGGCCA CGGAGTTCCT AGGTAGCGAT GGGCTGTTGG ATGAATGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Filamin-interacting proteins, Cfm1 and Cfm2, are essential for the formation of cartilaginous skeletal elements.
Human molecular genetics23(11)2953-67(2014 Jun)
Mizuhashi K,Kanamoto T,Moriishi T,Muranishi Y,Miyazaki T,Terada K,Omori Y,Ito M,Komori T,Furukawa T

RefilinB (FAM101B) targets filamin A to organize perinuclear actin networks and regulates nuclear shape.
Proceedings of the National Academy of Sciences of the United States of America108(28)11464-9(2011 Jul)
Gay O,Gilquin B,Nakamura F,Jenkins ZA,McCartney R,Krakow D,Deshiere A,Assard N,Hartwig JH,Robertson SP,Baudier J

cfm is a novel gene uniquely expressed in developing forebrain and midbrain, but its null mutant exhibits no obvious phenotype.
Gene expression patterns : GEP5(3)439-44(2005 Feb)
Hirano M,Murata T,Furushima K,Kiyonari H,Nakamura M,Suda Y,Aizawa S