Dhx33 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dhx33
Entrez Gene ID	287464
Full Name	DEAH-box helicase 33
General protein information	Preferred Names DEAH-box helicase 33 Names putative ATP-dependent RNA helicase DHX33 DEAH (Asp-Glu-Ala-His) box polypeptide 33
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q24

mRNA and Protein(s)

mRNA	Protein	Name
NM_001105802.1	NP_001099272.1	putative ATP-dependent RNA helicase DHX33

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	DHX33	XP_523842.2
Canis lupus familiaris (dog)	DHX33	XP_005619946.1
Rattus norvegicus (Norway rat)	Dhx33	NP_001099272.1
Mus musculus (house mouse)	Dhx33	NP_848144.3
Drosophila melanogaster (fruit fly)	CG4901	NP_609356.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006138	XP_556838.3
Xenopus tropicalis (tropical clawed frog)	dhx33	XP_002932324.1
Danio rerio (zebrafish)	LOC796505	NP_001093527.3
Homo sapiens (human)	DHX33	NP_064547.2
	DHX33	XP_001101396.2
Gallus gallus (chicken)	DHX33	XP_004946516.1

Gene Ontology
Bibliography

Related articles in PubMed

The DHX33 RNA Helicase Promotes mRNA Translation Initiation.
Zhang Y, You J, Wang X, Weber J
Molecular and cellular biology35(17)2918-31(2015 Sep)

The interaction between the helicase DHX33 and IPS-1 as a novel pathway to sense double-stranded RNA and RNA viruses in myeloid dendritic cells.
Liu Y, Lu N, Yuan B, Weng L, Wang F, Liu YJ, Zhang Z
Cellular & molecular immunology11(1)49-57(2014 Jan)

The DHX33 RNA helicase senses cytosolic RNA and activates the NLRP3 inflammasome.
Mitoma H, Hanabuchi S, Kim T, Bao M, Zhang Z, Sugimoto N, Liu YJ
Immunity39(1)123-35(2013 Jul)

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Castello A, Fischer B, Eichelbaum K, Horos R, Beckmann BM, Strein C, Davey NE, Humphreys DT, Preiss T, Steinmetz LM, Krijgsveld J, Hentze MW
Cell149(6)1393-406(2012 Jun)

Identification of DHX33 as a mediator of rRNA synthesis and cell growth.
Zhang Y, Forys JT, Miceli AP, Gwinn AS, Weber JD
Molecular and cellular biology31(23)4676-91(2011 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dhx33 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dhx33 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa11443	NM_001105802.1 Latest version!	Rattus norvegicus DEAH-box helicase 33 (Dhx33), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dhx33 ( NM_001105802.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001099272.1 Rattus norvegicus DEAH-box helicase 33 (Dhx33), mRNA.

Price & Availability↑

CloneID	ORa11443
Clone ID Related Accession (Same CDS sequence)	NM_001105802.1
Accession Version	NM_001105802.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2097bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-18
Organism	Rattus norvegicus(Norway rat)
Product	putative ATP-dependent RNA helicase DHX33
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473948.1. On or before Oct 3, 2007 this sequence version replaced XM_001080073.1, XM_213370.4. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGCCGGAGG AGGCGGGCCT CCCGCCCGCC AAGAGATTCC GGCCGGGCTC CTGCCCTCCC 
GGGAGGCGAG TAGTAATGCT GTTGACGGCT GGCAGCGGTG GCGGAGCAGG AGGAGGCCGG 
AGGCAGACGC CGCCCCTGGC CCAGCCTTCG GCCAGTCCCT ACCGCGAGGC CCTGGAGCTG 
CAGCGCCGGA GTCTGCCCAT CTTCCGGGCG CGGGGGCAGC TATTGGCACA GCTCCGAAAC 
CTGGACAACG CGGTCCTCAT CGGGGAAACT GGCTCTGGGA AGACAACTCA GATCCCACAG 
TACCTCTATG AAGGGGGAAT TAGCCGGCAG GGCATCATTG CTGTGACCCA GCCCCGTCGA 
GTGGCTGCCA TCTCTCTTGC CACTAGAGTC TCAGATGAGA AGAGGACTGA ACTCGGGAAG 
CTGGTTGGCT ATACAGTGCG CTTTGAGGAT GTCACCTCAG AAGATACTAG GATCAAGTTC 
CTGACAGATG GCATGCTTTT GCGTGAAGCA ATTTCAGATT CCTTGCTTCG GAAGTATAGC 
TGTGTCATTT TGGACGAGGC CCATGAGCGG ACTATCCACA CAGATGTGCT CTTTGGAGTA 
GTGAAGACTG CACAGAAGAG GCGAAAGGAG CTAGGGAAGC TGCCTCTCAA AGTGATTGTG 
ATGTCAGCTA CCATGGATGT TGACCTGTTT TCTCAGTATT TCAATGGCGC CCCTGTCCTC 
TACCTTGAGG GTCGGCAGCA TCCCATCCAG ATCTTTTATA CCAAACAGCC TCAGCAGGAT 
TACCTGCATG CAGCTCTTGT CTCAGTCTTC CAGATCCACC AGGAAGCCCC CTCCTCTCAG 
GACATCCTGG TCTTCCTCAC TGGTCAGGAG GAGATCGAAG CAATGAGCAA GACCTGCCGA 
GACATTGCAA AGCACCTCCC AGACGGCTGC CCTTCCATGC TCGTCCTTCC TCTGTACGCC 
TCCTTGCCCT ATTCACAGCA GCTTCGAGTC TTTCAAGGAG CCCCAAAGGG CTATCGCAAA 
GTGATCATTT CAACCAACAT CGCTGAGACC TCCATAACCA TTACAGGAAT AAAATACGTA 
GTTGACACCG GCATGGTTAA AGCAAAGAAG TATAACCCTG ACAGTGGTCT TGAGGTACTC 
GCTGTGCAGC GAGTGTCGAA GACCCAGGCC TGGCAGCGCA CAGGCCGGGC TGGACGGGAG 
GACAGCGGCA TCTGCTACCG GCTCTACACT GAGGACGAGT TTGACAAGTT TGAGAAGATG 
GCAGCACCAG AGATCCAGAG GTGTAACCTG GCAAGTGTGA TGCTTCAGCT CCTGGCAATG 
AAAGTCCCAA ATGTGCTCAC CTTTGACTTC ATGTCCAGAC CATCTCCAGA TCACATTGAG 
GCGGCCATTG CCCAACTGGA CCTGTTAGGT GCTCTTGAAC ATAAGGATGA CCAGCTTGCC 
CTGACTCCAA TCGGAAGAAA GATGGCAGCA TTTCCTTTAG AACCAAAATT TGCCAAAACT 
ATCCTCCTGT CCTCCAAATT CCACTGTACA GAAGAGATTC TGACCATAGT CTCCCTGCTG 
TCTGTAGACA GTGTCCTCTA CAACCCTCCT GCCCGGAGAG ATGAGGTGCA GAGTGTCCGG 
AAGAAATTCA TATCCAGTGA GGGGGATCAT ATCACCCTGC TCAATATCTA TCGGACCTTC 
AAAAACATAG GTGGGAATAA GGACTGGTGC AAAGAGAATT TTGTCAACAG CAAGAACATG 
CTCCTAGTAG CTGAAGTCAG AGCGCAGCTG AGGGACATCT GCTTAAAGAT GTCAATGCCA 
ATCATGTCAT CTCGAGGGGA CATGGAGAGT GTCCGCCGTT GCCTGGCTCA CAGCCTCTTT 
ATGAGTACTG CTGAACTCCA GCCAGATGGC ACCTATGCCA CCACAGACAC ACATCAGCCA 
GTGGCCATCC ACCCATCATC TGTCCTCTTC CACTGCAAGC CTGCCTGCGT GGTATACACT 
TCACTGCTCT ACACCAACAA ATGCTACATG CGTGACCTGT GTGTTGTGGA TGCCGAGTGG 
CTGTACGAGG CTGCCCCTGA CTACTTCAGG AGGAAGCTGA GAACGGCCAG AAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001099272.1
CDS	56..2152
Translation	MPEEAGLPPAKRFRPGSCPPGRRVVMLLTAGSGGGAGGGRRQTPPLAQPSASPYREALELQRRSLPIFRARGQLLAQLRNLDNAVLIGETGSGKTTQIPQYLYEGGISRQGIIAVTQPRRVAAISLATRVSDEKRTELGKLVGYTVRFEDVTSEDTRIKFLTDGMLLREAISDSLLRKYSCVILDEAHERTIHTDVLFGVVKTAQKRRKELGKLPLKVIVMSATMDVDLFSQYFNGAPVLYLEGRQHPIQIFYTKQPQQDYLHAALVSVFQIHQEAPSSQDILVFLTGQEEIEAMSKTCRDIAKHLPDGCPSMLVLPLYASLPYSQQLRVFQGAPKGYRKVIISTNIAETSITITGIKYVVDTGMVKAKKYNPDSGLEVLAVQRVSKTQAWQRTGRAGREDSGICYRLYTEDEFDKFEKMAAPEIQRCNLASVMLQLLAMKVPNVLTFDFMSRPSPDHIEAAIAQLDLLGALEHKDDQLALTPIGRKMAAFPLEPKFAKTILLSSKFHCTEEILTIVSLLSVDSVLYNPPARRDEVQSVRKKFISSEGDHITLLNIYRTFKNIGGNKDWCKENFVNSKNMLLVAEVRAQLRDICLKMSMPIMSSRGDMESVRRCLAHSLFMSTAELQPDGTYATTDTHQPVAIHPSSVLFHCKPACVVYTSLLYTNKCYMRDLCVVDAEWLYEAAPDYFRRKLRTARN

Target ORF information:

RefSeq Version	NM_001105802.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus DEAH-box helicase 33 (Dhx33), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001105802.1

ORF Insert Sequence:

ATGCCGGAGG AGGCGGGCCT CCCGCCCGCC AAGAGATTCC GGCCGGGCTC CTGCCCTCCC 
GGGAGGCGAG TAGTAATGCT GTTGACGGCT GGCAGCGGTG GCGGAGCAGG AGGAGGCCGG 
AGGCAGACGC CGCCCCTGGC CCAGCCTTCG GCCAGTCCCT ACCGCGAGGC CCTGGAGCTG 
CAGCGCCGGA GTCTGCCCAT CTTCCGGGCG CGGGGGCAGC TATTGGCACA GCTCCGAAAC 
CTGGACAACG CGGTCCTCAT CGGGGAAACT GGCTCTGGGA AGACAACTCA GATCCCACAG 
TACCTCTATG AAGGGGGAAT TAGCCGGCAG GGCATCATTG CTGTGACCCA GCCCCGTCGA 
GTGGCTGCCA TCTCTCTTGC CACTAGAGTC TCAGATGAGA AGAGGACTGA ACTCGGGAAG 
CTGGTTGGCT ATACAGTGCG CTTTGAGGAT GTCACCTCAG AAGATACTAG GATCAAGTTC 
CTGACAGATG GCATGCTTTT GCGTGAAGCA ATTTCAGATT CCTTGCTTCG GAAGTATAGC 
TGTGTCATTT TGGACGAGGC CCATGAGCGG ACTATCCACA CAGATGTGCT CTTTGGAGTA 
GTGAAGACTG CACAGAAGAG GCGAAAGGAG CTAGGGAAGC TGCCTCTCAA AGTGATTGTG 
ATGTCAGCTA CCATGGATGT TGACCTGTTT TCTCAGTATT TCAATGGCGC CCCTGTCCTC 
TACCTTGAGG GTCGGCAGCA TCCCATCCAG ATCTTTTATA CCAAACAGCC TCAGCAGGAT 
TACCTGCATG CAGCTCTTGT CTCAGTCTTC CAGATCCACC AGGAAGCCCC CTCCTCTCAG 
GACATCCTGG TCTTCCTCAC TGGTCAGGAG GAGATCGAAG CAATGAGCAA GACCTGCCGA 
GACATTGCAA AGCACCTCCC AGACGGCTGC CCTTCCATGC TCGTCCTTCC TCTGTACGCC 
TCCTTGCCCT ATTCACAGCA GCTTCGAGTC TTTCAAGGAG CCCCAAAGGG CTATCGCAAA 
GTGATCATTT CAACCAACAT CGCTGAGACC TCCATAACCA TTACAGGAAT AAAATACGTA 
GTTGACACCG GCATGGTTAA AGCAAAGAAG TATAACCCTG ACAGTGGTCT TGAGGTACTC 
GCTGTGCAGC GAGTGTCGAA GACCCAGGCC TGGCAGCGCA CAGGCCGGGC TGGACGGGAG 
GACAGCGGCA TCTGCTACCG GCTCTACACT GAGGACGAGT TTGACAAGTT TGAGAAGATG 
GCAGCACCAG AGATCCAGAG GTGTAACCTG GCAAGTGTGA TGCTTCAGCT CCTGGCAATG 
AAAGTCCCAA ATGTGCTCAC CTTTGACTTC ATGTCCAGAC CATCTCCAGA TCACATTGAG 
GCGGCCATTG CCCAACTGGA CCTGTTAGGT GCTCTTGAAC ATAAGGATGA CCAGCTTGCC 
CTGACTCCAA TCGGAAGAAA GATGGCAGCA TTTCCTTTAG AACCAAAATT TGCCAAAACT 
ATCCTCCTGT CCTCCAAATT CCACTGTACA GAAGAGATTC TGACCATAGT CTCCCTGCTG 
TCTGTAGACA GTGTCCTCTA CAACCCTCCT GCCCGGAGAG ATGAGGTGCA GAGTGTCCGG 
AAGAAATTCA TATCCAGTGA GGGGGATCAT ATCACCCTGC TCAATATCTA TCGGACCTTC 
AAAAACATAG GTGGGAATAA GGACTGGTGC AAAGAGAATT TTGTCAACAG CAAGAACATG 
CTCCTAGTAG CTGAAGTCAG AGCGCAGCTG AGGGACATCT GCTTAAAGAT GTCAATGCCA 
ATCATGTCAT CTCGAGGGGA CATGGAGAGT GTCCGCCGTT GCCTGGCTCA CAGCCTCTTT 
ATGAGTACTG CTGAACTCCA GCCAGATGGC ACCTATGCCA CCACAGACAC ACATCAGCCA 
GTGGCCATCC ACCCATCATC TGTCCTCTTC CACTGCAAGC CTGCCTGCGT GGTATACACT 
TCACTGCTCT ACACCAACAA ATGCTACATG CGTGACCTGT GTGTTGTGGA TGCCGAGTGG 
CTGTACGAGG CTGCCCCTGA CTACTTCAGG AGGAAGCTGA GAACGGCCAG AAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The DHX33 RNA Helicase Promotes mRNA Translation Initiation.
Molecular and cellular biology35(17)2918-31(2015 Sep)
Zhang Y,You J,Wang X,Weber J

The interaction between the helicase DHX33 and IPS-1 as a novel pathway to sense double-stranded RNA and RNA viruses in myeloid dendritic cells.
Cellular & molecular immunology11(1)49-57(2014 Jan)
Liu Y,Lu N,Yuan B,Weng L,Wang F,Liu YJ,Zhang Z

The DHX33 RNA helicase senses cytosolic RNA and activates the NLRP3 inflammasome.
Immunity39(1)123-35(2013 Jul)
Mitoma H,Hanabuchi S,Kim T,Bao M,Zhang Z,Sugimoto N,Liu YJ

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Cell149(6)1393-406(2012 Jun)
Castello A,Fischer B,Eichelbaum K,Horos R,Beckmann BM,Strein C,Davey NE,Humphreys DT,Preiss T,Steinmetz LM,Krijgsveld J,Hentze MW

Identification of DHX33 as a mediator of rRNA synthesis and cell growth.
Molecular and cellular biology31(23)4676-91(2011 Dec)
Zhang Y,Forys JT,Miceli AP,Gwinn AS,Weber JD

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Dhx33 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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