Sar1b cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sar1b
Entrez Gene ID	287276
Full Name	secretion associated, Ras related GTPase 1B
Synonyms	SARA1B,Sara2
General protein information	Preferred Names secretion associated, Ras related GTPase 1B Names GTP-binding protein SAR1b SAR1 gene homolog B SAR1 homolog B
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q22

mRNA and Protein(s)

mRNA	Protein	Name
NM_001009622.1	NP_001009622.1	GTP-binding protein SAR1b

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Sar1b	NP_001009622.1
Drosophila melanogaster (fruit fly)	sar1	NP_732718.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004098	XP_003436713.1
Caenorhabditis elegans (roundworm)	sar-1	NP_500582.1
Xenopus tropicalis (tropical clawed frog)	sar1b	NP_001015871.1
Bos taurus (cattle)	SAR1B	NP_001030392.1
Danio rerio (zebrafish)	sar1b	NP_001019548.1
Arabidopsis thaliana (thale cress)	SAR2	NP_192117.1
Pan troglodytes (chimpanzee)	SAR1B	XP_001167618.1
	SAR1B	XP_001109899.1
Homo sapiens (human)	SAR1B	NP_057187.1
Canis lupus familiaris (dog)	SAR1B	XP_538630.2
Oryza sativa (rice)	Os01g0254000	NP_001042615.1
Mus musculus (house mouse)	Sar1b	NP_079811.1
Gallus gallus (chicken)	SAR1B	NP_001025792.1
Arabidopsis thaliana (thale cress)	AT3G62560	NP_191815.1

Gene Ontology
Bibliography

Related articles in PubMed

Sar1 translocation onto the ER-membrane for vesicle budding has different pathways for promotion and suppression of ER-to-Golgi transport mediated through H89-sensitive kinase and ER-resident G protein.
Nakagawa H, Ishizaki M, Miyazaki S, Abe T, Nishimura K, Komori M, Matsuo S
Molecular and cellular biochemistry366(1-2)175-82(2012 Jul)

Phosphorylation of Sar1b protein releases liver fatty acid-binding protein from multiprotein complex in intestinal cytosol enabling it to bind to endoplasmic reticulum (ER) and bud the pre-chylomicron transport vesicle.
Siddiqi S, Mansbach CM 2nd
The Journal of biological chemistry287(13)10178-88(2012 Mar)

ER-resident Gi2 protein controls sar1 translocation onto the ER during budding of transport vesicles.
Nakagawa H, Umadome H, Miyazaki S, Tanaka K, Nishimura K, Komori M, Matsuo S
Journal of cellular biochemistry112(9)2250-6(2011 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Sar1 recruitment to the endoplasmic reticulum-membrane is sensitive to H89-sensitive kinase activation, indicating the first step in transport vesicle formation occurs under kinase regulation
Title: Sar1 translocation onto the ER-membrane for vesicle budding has different pathways for promotion and suppression of ER-to-Golgi transport mediated through H89-sensitive kinase and ER-resident G protein.

Phosphorylation of Sar1b protein releases liver fatty acid-binding protein from multiprotein complex in intestinal cytosol enabling it to bind to endoplasmic reticulum (ER) and bud the pre-chylomicron transport vesicle.
Title: Phosphorylation of Sar1b protein releases liver fatty acid-binding protein from multiprotein complex in intestinal cytosol enabling it to bind to endoplasmic reticulum (ER) and bud the pre-chylomicron transport vesicle.

Sar1 translocation onto the endoplasmic reticulum was modulated by endoplasmic reticulum-resident G(i2)
Title: ER-resident Gi2 protein controls sar1 translocation onto the ER during budding of transport vesicles.

The following Sar1b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sar1b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa13692	NM_001009622.1 Latest version!	Rattus norvegicus secretion associated, Ras related GTPase 1B (Sar1b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sar1b ( NM_001009622.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001009622.1 Rattus norvegicus secretion associated, Ras related GTPase 1B (Sar1b), mRNA.

Price & Availability↑

CloneID	ORa13692
Clone ID Related Accession (Same CDS sequence)	NM_001009622.1
Accession Version	NM_001009622.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 597bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-16
Organism	Rattus norvegicus(Norway rat)
Product	GTP-binding protein SAR1b
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC088842.1. On Jan 13, 2005 this sequence version replaced XM_213283.2. ##Evidence-Data-START## Transcript exon combination :: BC088842.1, FQ217751.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGTCCTTCA TATTTGACTG GATTTACAGT GGTTTCAGCA GTGTGCTGCA GTTTCTAGGA 
TTGTATAAAA AAACTGGTAA ACTGGTATTC CTTGGATTGG ATAATGCCGG GAAAACAACG 
TTGCTACACA TGCTGAAAGA TGACAGGCTT GGCCAGCACG TCCCAACACT ACATCCCACT 
TCAGAGGAGC TCACTATTGC TGGCATGACG TTTACAACTT TTGATCTGGG TGGGCATTTG 
CAAGCCCGAA GAGTATGGAA AAACTACCTT CCTGCTATCA ATGGCATTGT ATTTCTTGTG 
GATTGTGCAG ACCATGAAAG GCTGTTAGAA TCAAAAGAAG AACTTGATTC ACTAATGACA 
GATGAAACCA TTGCCAACGT GCCTATCCTA ATTCTTGGAA ACAAGATCGA CAGGCCTGAA 
GCCATAAGTG AAGAGAGGTT ACGAGAGATG TTTGGTTTAT ATGGTCAGAC AACAGGAAAG 
GGCAGTGTGT CACTGAAGGA GCTGAATGCC CGGCCTCTGG AAGTGTTCAT GTGCAGTGTG 
CTAAAAAGGC AGGGCTACGG GGAAGGCTTC CGCTGGATGG CGCAGTACAT CGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001009622.1
CDS	89..685
Translation	MSFIFDWIYSGFSSVLQFLGLYKKTGKLVFLGLDNAGKTTLLHMLKDDRLGQHVPTLHPTSEELTIAGMTFTTFDLGGHLQARRVWKNYLPAINGIVFLVDCADHERLLESKEELDSLMTDETIANVPILILGNKIDRPEAISEERLREMFGLYGQTTGKGSVSLKELNARPLEVFMCSVLKRQGYGEGFRWMAQYID

Target ORF information:

RefSeq Version	NM_001009622.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus secretion associated, Ras related GTPase 1B (Sar1b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001009622.1

ORF Insert Sequence:

ATGTCCTTCA TATTTGACTG GATTTACAGT GGTTTCAGCA GTGTGCTGCA GTTTCTAGGA 
TTGTATAAAA AAACTGGTAA ACTGGTATTC CTTGGATTGG ATAATGCCGG GAAAACAACG 
TTGCTACACA TGCTGAAAGA TGACAGGCTT GGCCAGCACG TCCCAACACT ACATCCCACT 
TCAGAGGAGC TCACTATTGC TGGCATGACG TTTACAACTT TTGATCTGGG TGGGCATTTG 
CAAGCCCGAA GAGTATGGAA AAACTACCTT CCTGCTATCA ATGGCATTGT ATTTCTTGTG 
GATTGTGCAG ACCATGAAAG GCTGTTAGAA TCAAAAGAAG AACTTGATTC ACTAATGACA 
GATGAAACCA TTGCCAACGT GCCTATCCTA ATTCTTGGAA ACAAGATCGA CAGGCCTGAA 
GCCATAAGTG AAGAGAGGTT ACGAGAGATG TTTGGTTTAT ATGGTCAGAC AACAGGAAAG 
GGCAGTGTGT CACTGAAGGA GCTGAATGCC CGGCCTCTGG AAGTGTTCAT GTGCAGTGTG 
CTAAAAAGGC AGGGCTACGG GGAAGGCTTC CGCTGGATGG CGCAGTACAT CGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sar1 translocation onto the ER-membrane for vesicle budding has different pathways for promotion and suppression of ER-to-Golgi transport mediated through H89-sensitive kinase and ER-resident G protein.
Molecular and cellular biochemistry366(1-2)175-82(2012 Jul)
Nakagawa H,Ishizaki M,Miyazaki S,Abe T,Nishimura K,Komori M,Matsuo S

Phosphorylation of Sar1b protein releases liver fatty acid-binding protein from multiprotein complex in intestinal cytosol enabling it to bind to endoplasmic reticulum (ER) and bud the pre-chylomicron transport vesicle.
The Journal of biological chemistry287(13)10178-88(2012 Mar)
Siddiqi S,Mansbach CM 2nd

ER-resident Gi2 protein controls sar1 translocation onto the ER during budding of transport vesicles.
Journal of cellular biochemistry112(9)2250-6(2011 Sep)
Nakagawa H,Umadome H,Miyazaki S,Tanaka K,Nishimura K,Komori M,Matsuo S

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

Sar1b cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報